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Oxidative stress in Microcystis aeruginosa as a consequence of global climate change

BIOCELL 2016年第1期40卷 23-25页

作者： MARCELO HERNANDO CHRISTIAN HOUGHTON leda giannuzzi BERND KROCK DARIO ANDRINOLO GABRIELA MALANGA Departamento de Radiobiología Comisión Nacional de Energía AtómicaBuenos AiresArgentina Area de Toxicología Dpto.de Cs BiológicasFacultad de Ciencias Exactas.UNLP Alfred Wegener Institute BremerhavenGermany Instituto de Bioquímica y Medicina Molecular(IBIMOL)-Universidad de Buenos Aires(UBA) CONICETFisicoquímicaFacultad de Farmacia y BioquímicaJunín 956(C1113AAD)Buenos Aires.Argentina

Cyanobacteria are phototrophic organisms with great ecological and economical *** of the genus Microcystis are known for their potential ability to synthesize toxins,notably *** is a growing interest in the evaluation of oxidative stress in relation to the impact of global climate change on natural ecosystems in different trophic *** studies have focused on the analysis of organismal responses to mitigate the damage by controlling the generation of reactive oxygen *** in environ-mental factors caused by climate change generate a situation of oxidative damage in Microcystis aeruginosa as a direct or indirect *** this study we evaluate the effects of ultraviolet radiation and temperature on physiological and biochemical responses of a native ***(strain CAAT 2005-3).The results from the exposure to ultraviolet radiation doses and temperature changes suggest a high ability of *** to detect a potential stress situation as a consequence of reactive species production and to rapidly initiate antioxidant *** catalase activity is an antioxidant protection mechanism in *** for short and long term exposure to different changes in environmental ***,we found a ultraviolet-B radiation threshold dose above which oxidative stress exceeds the antioxidant protection and damage *** addition our results are in agreement with recent findings suggesting that microcystins may act as protein-modulating metabolites and protection against reactive oxygen *** is concluded that cyanobacteria have adaptative mechanisms that could lead to the replacement of species highly susceptible to oxidative stress by others with a higher system of antioxidant protection.

关键词： oxidative stress Cyanobacteria UVR temperature

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The Use of Chloride, Citric and Ascorbic Acid Dip and Packaged Film to Extend the Shelf Life of Pejerrey (<i>Odonthested bonaerensis</i>) during Storage at Different Temperatures

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Food and Nutrition Sciences 2014年第15期5卷 1506-1520页

作者： Copes Julio Pellicer Karina Bigeon Giselda giannuzzi leda Cátedra de Tecnología y Sanidad de los Alimentos Facultad de Ciencias Veterinarias Universidad Nacional de La Plata La Plata Argentina Centro de Investigación y Desarrollo en Criotecnología de Alimentos (CIDCA) CONICET Universidad Nacional de La Plata La Plata Argentina

The objective of this study was to analyze the effect of chloride, citric and ascorbic acid dip treatments in conjunction with two types of packaging films (with high and low gas permeability) on microbial growth in samples of pejerrey fillets (Odonthestes bonaerensis) at three storage temperatures (4°C, 0°C and -1.5°C). Colour, pH, texture modification and chemical changes were also studied. Psychrotrophic microorganisms, sp. were modelled by the Gompertz’ equation. Lag phase duration, specific growth rate and maximum population density were calculated. The activation energy (Eµ) was calculated reaching values of 147 and 177 kJ/mol when the low permeability packaging films were employed. We used psychrotrophic microorganisms, sp., as a predictor of the shelf life of product. The application of treatment with chloride, citric and ascorbic acids, extended 1 - 2 days the storage life of the product (time to reach 106 CFU/g) when the packaging material used was polyethylene. The use of chloride, citric and ascorbic acids and vacuum packaging produced shelf life values of 7.7, >25 and >25 days at 4°C, 0°C and -1.5°C respectively. The products had very good organoleptic characteristics, maintained their colour and texture appropriate, and absence of pathogenic microorganisms. These values indicated that the growth models were acceptable for expressing the growth of microorganisms on pejerrey fillets, which can be applied to ensure the safety of fish and to establish standards for avoiding microbial contamination.

关键词： Mathematical Modeling Preservatives Vacuum-Packaging Fish Spoilage Storage Life Chill and Superchilling Storage

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Application of Whey Permeate Fermented with Kefir Grains for the Shelf-Life Improvement of Food and Feed

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Advances in Microbiology 2016年第9期6卷 650-661页

作者： Raúl Ricardo Gamba Candela Moure Gabriela Diosma leda giannuzzi Graciela Liliana De Antoni Ángela María León Peláez Cátedra de Microbiología Facultad de Ciencias Exactas Universidad Nacional de La Plata La Plata Argentina CIDCA (Centro de Investigación y Desarrollo en Criotecnología de los Alimentos) La Plata Argentina CONICET (Consejo Nacional de Investigaciones Científicas y Tecnológicas) CCT-La Plata La Plata Argentina Cátedra de Toxicología Facultad de Ciencias Exactas Universidad Nacional de La Plata La Plata Argentina CIC-PBA (Comisión de Investigaciones Científicas-Provincia de Buenas Aires) Buenas Aires Argentina

Fungal contamination by Aspergillus parasiticus and A. flavus causes negative effects on the production of food cereals. Kefir is an ancient fermented beverage obtained by the fermentation of different substrates with kefir grains. An important waste produced by the dairy cheese industry is the whey permeate, which nowadays is a strong ambient contaminant. The aim of this work was the standardization of the whey permeate fermenting conditions with kefir grains, the assessment of the antifungal activity of the cell-free-supernatants (CFS) from these fermentations and to compare it with that obtained with CFS of milk fermented with kefir grains. Finally, we studied if the addition of kefir fermented whey permeate to food (bread) and feed (poultry) could produce shelf life improvement. The optimal condition to obtain CFS with fungicidal effect was a fermentation with 10% w/v of kefir grains, at 30°C, for 24 hours until a pH 3.7. We found that CFS from whey permeate caused fungal inhibition, whereas CFS of kefir grains grown in milk showed lower antifungal activity. Additionally, the addition of kefir-fermented whey permeates in food (bread) and feed (poultry) improved their resistance to fungal contamination. This is the first report about the application of kefir-fermented whey permeate to improve the shelf life, suggesting its potential use as a biopreservative.

关键词： Aspergillus parasiticus Whey Permeate Kefir Supernatants Bread Poultry Feed

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Microcystis aeruginos strain[D-Leu^(1)]Mcyst-LR producer,from Buenos Aires province,Argentina

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Journal of Coastal Life Medicine 2014年第4期 287-296页

作者： Lorena Rosso Daniela Sedan Maria Kolman Josep Caixach Cintia Flores Juan Manuel Oteiza Graciela Salerno Ricardo Echenique leda giannuzzi Darío Andrinolo Cátedra de Toxicología Facultad de Ciencias ExactasUniversidad Nacional de La Plata48 y 115(1900)La PlataArgentina CEBB-CIB Fundación para Investigaciones Biológicas Aplicadas(FIBA-Mar del Plata) Mar del PlataArgentina Laboratori d'Espectrometria de Masses/Contaminants Orgànics Institut de Diagnosi Ambiental i Estudis de l'Aigua(IDAEA)CSIC.J.Girona18-2608034-BarcelonaSpain División Ficología Facultad de Ciencias Naturales y MuseoUniversidad Nacional de La PlataPaseo del Bosque s/n°(1900)La PlataArgentina

Objective:To show the toxicological and phylogenetic characterization of a native Microcystis ceruginosa(***)strain(named CAAT 2005-3)isolated from a water body of Buenos Aires province,***:A *** strain was isolated from the drainage canal of the sewage treatment in the town of Pila,Buenos Aires province,Argentina and acclimated to laboratory *** amplification of cpcBA-IGS Phcocyanin(PC,intergenic spacer and flanking regions)was carried out in order to build a phylogenetic *** exactive/orbitrap mass spectrometer equipped with an electrospray ionization source(Thermo Fisher Scientific,Bremen,Germany)was used for the LC/ESI-HRMS microcystins *** number of cell/mL and[D-Leu^(1)]Mcyst-LR production obtained as a function of time was modelled using the Gompertz ***:The phylogenetic analysis showed that the sequence clustered with others *** sequences obtained from *** first Argentinian strain of ***(CAAT 2005-3)growing under culture conditions maintains the typical colonial architecture of *** with profuse *** CAAT 2005-3 expresses a toxin variant,that was identified by LC-HRMs/Orbitrapas as[D-Leu^(1)]microcystin-LR([M+H]+=1037.8 m/z).Conclusions:[D-Leu^(1)]microcystin-LR has been also detected in *** samples from Canada,Brazil and *** work provides the basis for technological development and production of analytical standards of toxins present in our region.

关键词： Cyanobacteria Microcystis aeruginosa [D-Leu^(1)]microcystin-LR[M+H]+ Harmful algal blooms

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Practical Implementation and Operational Experience of Dynamic Mode Decomposition in Wide-area Monitoring Systems of Italian Power System

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Journal of Modern Power Systems and Clean Energy 2023年第3期11卷 793-802页

作者： Andrea Vicario Alberto Berizzi Giorgio Maria giannuzzi Cosimo Pisani Department of Energy at Politecnico di Milano MilanItaly Terna S.p.A. RomeItaly

This study presents the assumptions and strategies for the practical implementation of the dynamic mode decomposition approach in the wide-area monitoring system of the Italian transmission system operator,*** procedure setup aims to detect poorly damped interarea oscillations of power *** mode decomposition is a data-driven technique that has gained increasing attention in different fields;the proposed implementation can both characterize the oscillatory modes and identify the most influenced *** study presents the results of its practical implementation and operational experience in power system *** focuses on the main characteristics and solutions identified to reliably monitor the interarea electromechanical modes of the interconnected European power ***,conditions to issue an appropriate alarm in case of critical operating conditions are *** effectiveness of the proposed approach is validated by its application in three case studies:a critical oscillatory event and a short-circuit event that occurred in the Italian power system in the previous years,and a 15-min time interval of normal grid operation recorded in March 2021.

关键词： Power system control power system dynamics wide-area monitoring system(WAMS) dynamic mode decomposition

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Transcriptome‑wide mapping of milk somatic cells upon subclinical mastitis infection in dairy cattle

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Journal of Animal Science and Biotechnology 2023年第6期14卷 2630-2648页

作者： Vittoria Bisutti Nuria Mach Diana giannuzzi Alice Vanzin Emanuele Capra Riccardo Negrini Maria Elena Gelain Alessio Cecchinato Paolo Ajmone‑Marsan Sara Pegolo DAFNAE University of PadovaViale Dell’Universita 16LegnaroPD 35020Italy. IHAP Universite de ToulouseINRAEENVT23 Chemin Des CapellesToulouse 31300France. IBBA National Research CouncilVia EinsteinLodi 26900Italy. DIANA Universita Cattolica del Sacro CuoreVia E.Parmense 84Piacenza 29122Italy. BCA University of PadovaViale Dell’Universita 16LegnaroPD 35020Italy.

Background Subclinical intramammary infection(IMI)represents a significant problem in maintaining dairy cows’*** severity and extent depend on the interaction between the causative agent,environment,and *** investigate the molecular mechanisms behind the host immune response,we used RNA-Seq for the milk somatic cells(SC)transcriptome profiling in healthy cows(n=9),and cows naturally affected by subclinical IMI from Proto-theca spp.(n=11)and Streptococcus agalactiae(***;n=11).Data Integration Analysis for Biomarker discov-ery using Latent Components(DIABLO)was used to integrate transcriptomic data and host phenotypic traits related to milk composition,SC composition,and udder health to identify hub variables for subclinical IMI *** A total of 1,682 and 2,427 differentially expressed genes(DEGs)were identified when comparing Prototheca *** *** to healthy animals,***-specific pathway analyses evidenced that Proto-theca’s infection upregulated antigen processing and lymphocyte proliferation pathways while *** induced a reduction of energy-related pathways like the tricarboxylic acid cycle,and carbohydrate and lipid *** integrative analysis of commonly shared DEGs between the two pathogens(n=681)referred to the core-mastitis response genes,and phenotypic data evidenced a strong covariation between those genes and the flow cytometry immune cells(r2=0.72),followed by the udder health(r2=0.64)and milk quality parameters(r2=0.64).Variables with r≥0.90 were used to build a network in which the top 20 hub variables were identified with the Cytoscape cyto-hubba *** genes in common between DIABLO and cytohubba(n=10)were submitted to a ROC analysis which showed they had excellent predictive performances in terms of discriminating healthy and mastitis-affected animals(sensitivity>0.89,specificity>0.81,accuracy>0.87,and precision>0.69).Among these genes,CIITA could play a key role in re

关键词： Data integration Immune response Milk somatic cells RNA-sequencing Subclinical mastitis

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The“A”of FAIR-As Open as Possible,as Closed as Necessary

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Data Intelligence 2020年第1期2卷 47-55,303,304页

作者： Annalisa Landi Mark Thompson Viviana giannuzzi Fedele Bonifazi Ignasi Labastida Luiz Olavo Bonino da Silva Santos Marco Roos Fondazione per la Ricerca Farmacologica Gianni Benzi Onlus 30-70010 Valenzano(BA)Italy Leiden University Medical Center Leiden2333 ZAThe Netherlands Learning and Research Resources Centre(CRAI) Universitat de BarcelonaCatalunya 08007Spain GO FAIR International Support&Coordination Office(GFISCO) LeidenThe Netherlands

In order to provide responsible access to health data by reconciling benefits of data sharing with privacy rights and ethical and regulatory requirements,Findable,Accessible,Interoperable and Reusable(FAIR)metadata should be *** to the H2020 Program Guidelines on FAIR Data,data should be"as open as possible and as closed as necessary","open"in order to foster the reusability and to accelerate research,but at the same time they should be"closed"to safeguard the privacy of the *** provisions on the protection of natural persons with regard to the processing of personal data have been endorsed by the European General Data Protection Regulation(GDPR),Reg(EU)2016/679,that came into force in May *** work aims to solve accessibility problems related to the protection of personal data in the digital era and to achieve a responsible access to and responsible use of health *** strongly suggest associating each data set with FAIR metadata describing both the type of data collected and the accessibility conditions by considering data protection obligations and ethical and regulatory ***,an existing FAIR infrastructure component has been used as an example to explain how FAIR metadata could facilitate data sharing while ensuring protection of individuals.

关键词： Accessibility of health data FAIR metadata GDPR Data sharing ELSI

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墨西哥能否抓佳投资热潮

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商业周刊（中文版） 2023年第17期 30-33页

作者： Maya Averbuch leda Alvim Felipe Hernandez Heitor Caixeta 晴川(译) 不详

●墨西哥已成为对美出口最多的国家,如何将投资热潮对经济的推动作用维持下去是个挑战墨西哥刚刚超越中国,成为美国这个庞大邻国的最大商品供应商。除了出口再次升温,墨西哥今年汇率走势也是全球最强,且股市表现强劲。2023年,其外商直... 详细信息

●墨西哥已成为对美出口最多的国家,如何将投资热潮对经济的推动作用维持下去是个挑战墨西哥刚刚超越中国,成为美国这个庞大邻国的最大商品供应商。除了出口再次升温,墨西哥今年汇率走势也是全球最强,且股市表现强劲。2023年,其外商直接投资额增长40%以上,这还是在特斯拉拟议修建的50亿美元工厂开工之前的数据。自上世纪90年代签署《北美自由贸易协定》以来,墨西哥对投资者的吸引力从未像现在这样大过。

关键词：外商直接投资额汇率走势特斯拉《北美自由贸易协定》对美出口供应商股市表现墨西哥

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Calculation of the Zeeman-Fine Energies and the Spectrum with Doppler-Shift Correction of Atomic Lithium

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Journal of Modern Physics 2011年第7期2卷 752-758页

作者： Laila Babsail leda Bousiakou Salwa Alsaleh Mesude Saglam 不详

We have calculated the Zeeman-fine energies of atomic Lithium (Li) by using the varying effective Landé g-factor method. We take the principle quantum number in the range;(2 ≤n ≤10 ). For this range we find 26 different energy values and 325 wavelengths some of which are the same. The Doppler shift is found to be Δλ=±0.004λ. The Doppler shift-corrected wavelengths are in perfect agreement with the observed (NIST) values for atomic Li.

关键词： Hydrogen-Like Atoms Effective Landé G-Factor Quantum Entanglement Zeeman-Fine Energies Photonic Transitions Quantum Flux Of Photon

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Non-celiac gluten sensitivity:Time for sifting the grain

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世界胃肠病学杂志：英文版 2015年第27期21卷 8221-8226页

作者： Luca Elli leda Roncoroni Maria Teresa Bardella Center for Prevention and Diagnosis of Celiac Disease Gastroenterology and Endoscopy Unit Department of Pathophysiology and Transplantation Università degli Studi di Milano. Fondazione IRCCS Ca’ Granda Ospedale Maggiore Policlinico

In the last few years, a new nomenclature has been proposed for the disease induced by the ingestion of gluten, a protein present in wheat, rice, barley and oats. Besides celiac disease and wheat allergy, the most studied forms of gluten-related disorders characterized by an evident immune mechanism(autoimmune in celiac disease and Ig E-mediated in wheat allergy), a new entity has been included, apparently not driven by an aberrant immune response: the non-celiac gluten sensitivity(NCGS). NCGS is characterized by a heterogeneous clinical picture with intestinal and extraintestinal symptoms arising after gluten ingestion and rapidly improving after its withdrawal from the diet. The pathogenesis of NCGS is largely unknown, but a mixture of factors such as the stimulation of the innate immune system, the direct cytotoxic effects of gluten, and probably the synergy with other wheat molecules, are clues for the complicated puzzle. In addition, the diagnostic procedures still remain problematic due to the absence of efficient diagnostic markers; thus, diagnosis is based upon the symptomatic response to a gluten-free diet and the recurrence of symptoms after gluten reintroduction with the possibility of an important involvement of a placebo effect. The temporary withdrawal of gluten seems a reasonable therapy, but the timing of gluten reintroduction and the correct patient management approach are have not yet been determined.

关键词： Celiac disease Gluten Gluten-relateddisorders Gluten sensitivity Non-celiac gluten sensitivity

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