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The Effects of Nutritional Media and Initial Cell Density on the Growth and Development of Spirulina platensis

Journal of Agricultural Science and Technology(A) 2017年第B10期7卷 60-67页

作者： Nguyen Van Khanh Nguyen thi Diem le thi tuyet nhan Phan Van Cu Tran Quang Khanh Van Ngo thi Hoan Institute of Biotechnology Hue UniversityPhu ThuongPhu VangThua Thien Hue 530000Vietnam Faculty of Fisheries University of Agriculture and ForestryHue University102 Phung Hung StreetHue 530000Vietnam Faculty of Fisheries Ha Long University258 Bach Dang StreetUong Bi City 207900Quang NinhVietnam

The main objective of the study was to determine the effects of nutritional media and initial cell density on the growth and development of Spirulina *** study was carried out at the Center for Incubation and Technology Transfer,Hue University from May 2015 to August *** the first experiment,*** was cultured in four different nutritional media,including Zarrouk,Spi-RIA3,Nisole and Thuoc medium(Vietnamese).Resulted showed that *** grew significantly in the Zarrouk medium rather than in other nutritional medium at the same time,and gained the maximum density of 2,736.5 mg/L after 13 d inoculums,with specific growth rate of 4.987%per day and the time to doubling population of 0.139 *** the second experiment,*** was cultured in six different initial cell densities:100,400,700,1,000,1,300 and 1,600 mg/L,and the optimum nutritional medium was Zarrouk medium selected from experiment *** showed at the initial culture density of 400 mg/L,the highest biomass was gained with the maximum density of 3,071.2 mg/L after 21 d inoculums,with specific growth rate of 9.15%per day and time to double population of 0.076 *** biomass from culture in Zarrouk medium at initial cell density 400 mg/L obtained total protein of 51.98%,total lipid of 1.75%,total carbohydrate of 0.75%,carotene of 0.36%and total minerals of 5.2%,on a basis of dry matter.

关键词： Spirulina platensis nutrient medium initial density growth rates

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