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A reproducible and sensitive method for generating high-quality transcriptomes from single whitefly salivary glands and other low-input tissues

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Insect Science 2022年第5期29卷 1318-1328页

作者： Marco Gebiola Brandon h.le Kerry EMauck Department of Entomology University of California RiversideRiversideCalifornia Department of Botany and Plant Sciences University of California RiversideRiversideCalifornia Institute of Integrative Genome Biology University of California RiversideRiversideCaliforniaUSA

Transcriptomic studies are an important tool for understanding the molecular pathways underlying host plant use by agricultural pests,including vectors of damaging plant *** far,bulk RNA-Seq has been the main approach for non-model *** method relies on pooling large numbers of whole organisms or hundreds of individually dissected *** latter approach is logistically challenging,may introduce artifacts of handling and storage,and is not compatible with biological ***,we tested an approach to generate transcriptomes of individual salivary glands and other low-input body tissues from whiteflies(Bemisia tabaci MEAM1),which are major vectors of plant *** comparing our outputs to published bulk RNA-Seq datasets for whole whitefly bodies and pools of salivary glands,we demonstrate that this approach recovers similar numbers of transcripts relative to bulk RNA-Seq in a tissue-specific manner,and for some metrics,exceeds performance of bulk tissue *** generated from individual salivary glands also yielded additional novel transcripts not identified in pooled salivary gland datasets,and had hundreds of enriched transcripts when compared with whole head ***,our study demonstrates that it is feasible to produce high quality,replicated transcriptomes of whitefly salivary glands and other low-input *** anticipate that our approach will expand hypothesis-driven research on salivary glands of whiteflies and other hemiptera,thus enabling novel control strategies to disrupt feeding and virus transmission.

关键词： Aleyrodidae Bemisia tabaci bulk RNA-Seq insect vectors low-input RNA-Seq plant pathogens

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Mathematical modeling and numerical computation of the effective interfacial conditions for Stokes flow on an arbitrarily rough solid surface

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Applied Mathematics and Mechanics(English Edition) 2021年第5期42卷 721-746页

作者： A.T.TRAN h.le QUANG Q.C.hE D.h.NGUYEN Research and Application Center for Technology in Civil Engineering University of Transport and CommunicationsHanoi 10000Vietnam Laboratoire Modelisation et Simulation Multi-Echelle Unite Mixte de Recherche 8208Centre National de la Recherche ScientifiqueUniversite Gustave EiffelMarne-la-Vallee F-77454France School of Mechanical Engineering Southwest Jiaotong UniversityChengdu 610031China

The present work is concerned with a two-dimensional(2D)Stokes flow through a channel bounded by two parallel solid *** distance between the walls may be arbitrary,and the surface of one of the walls can be arbitrarily *** main objective of this work consists in homogenizing the heterogeneous interface between the rough wall and fluid so as to obtain an equivalent smooth slippery fluid/solid interface characterized by an effective slip *** solve the corresponding problem,two efficient numerical approaches are elaborated on the basis of the method of fundamental solution(MFS)and the boundary element methods(BEMs).They are applied to different cases where the fluid/solid interface is periodically or randomly *** results obtained by the proposed two methods are compared with those given by the finite element method and some relevant ones reported in the *** comparison shows that the two proposed methods are particularly efficient and accurate.

关键词： effective slip length method of fundamental solution(MFS) boundary element method(BEM) Stokeslet micro-channel fluid/solid interface

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A Statistical Study on Seismo-Ionospheric Anomalies of the Total Electron Content for the Period of 56 M≥6.0 Earthquakes Occurring in China During 1998—2012

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空间科学学报 2013年第3期33卷 258-269页

作者： Liu J Y Chen C h Tsai h F le h Institute of Space Science National Central University Center for Space and Remote Sensing Research National Central University Department of Earth Science National Cheng Kung University GPS Science and Application Research Center National Central University Key Laboratory of Ionospheric Environment Institute of Geology and GeophysicsChinese Academy of Sciences

This paper reports statistical results of Seismo-Ionospheric Anomalies(SIAs) of the Total Electron Content(TEC) in the Global Ionosphere Map(GIM) associated with 56 M≥6.0 earthquakes in China during 1998—*** detect SIA,a quartile-based(***-based) process is *** anomalies for the period of earthquakes without being led by magnetic storms about 10 days are further isolated and examined to confirm the SIP *** show that SIA is the TEC significantly decrease in the afternoon period 2—9 days before the earthquakes in China,which is in a good agreement with the SIA appearing before the 12 May 2008 M 8.0 Wenchuan earthquake.

关键词：电离层异常地震发生总电子含量中国统计 SIAS TEC GIM

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Giant non-linear susceptibility of hydrogenic donors in silicon and germanium

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Light(Science & Applications) 2019年第1期8卷 624-630页

作者： Nguyen h.le Grigory V.Lanskii Gabriel Aeppli Benedict N.Murdin Advanced Technology Institute and SEPNet University of SurreyGuildford GU27XHUK Institute of Monitoring of Climatic and Ecological Systems SB RAS 10/3 Academical Ave.Tomsk 634055Russia Laboratory for Solid State Physics ETH ZurichZurich CH-8093Switzerland Institut de Physique EPF LausanneLausanne CH-1015Switzerland Paul Scherrer Institut VilligenPSI CH-5232Switzerland

Implicit summation is a technique for the conversion of sums over intermediate states in multiphoton absorption and the high-order susceptibility in hydrogen into simple ***,we derive the equivalent technique for hydrogenic impurities in multi-valley *** the absorption has useful applications,it is primarily a loss process;conversely,the non-linear susceptibility is a crucial parameter for active photonic *** Si:P,we predict the hyperpolarizability ranges from χ^((3))/n_(3D)=2.9 to 580×10^(−38 )m^(5)/V^(2) depending on the frequency,even while avoiding *** samples of a reasonable density,n3D,and thickness,L,to produce thirdharmonic generation at 9 Thz,a frequency that is difficult to produce with existing solid-state sources,we predict that χ^((3)) should exceed that of bulk InSb and χ^((3))L should exceed that of graphene and resonantly enhanced quantum wells.

关键词： susceptibility hydrogenic absorption

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Thrombogenicity of biodegradable metals

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Bioactive Materials 2024年第8期38卷 411-421页

作者： D.E.J.Anderson h.h.le h.Vu J.Johnson J.E.Aslan J.Goldman M.T.hinds Department of Biomedical Engineering Oregon Health&Science UniversityPortlandORUSA Knight Cardiovascular Institute Oregon Health&Science UniversityPortlandORUSA Department of Biomedical Engineering Michigan Technological UniversityHoughtonMIUSA

Biodegradable metals offer a promising means to ameliorate many of the long-term risks associated with vascular devices made of conventional biostable stent *** numerous biodegradable metal alloys have been developed and characterized in animal models,knowledge of their blood reactivity and thrombogenicity remains *** hemocompatibility is particularly valuable because current generation drug-eluting stents pose a significant long-term thrombosis *** this study,four pure metals,widely used as degradable base materials(Fe,Zn,Mg,and Mo),and three alloys commonly used in cardiovascular devices[NiTi,CoCr,and stainless steel(SS)]were *** work examined how each of these metals activate platelets,coagulation factors,and inflammation using in vitro hemocompatibility assays and a clinically relevant ex vivo non-human primate arteriovenous shunt *** found that while all metals promoted a downstream activation of platelets and coagulation in flowing whole blood,platelet and fibrin attachment to Mg was markedly ***,Fe and Mo trended toward higher platelet attachment and contact pathway ***,the results suggest that Mg may delay clot initiation,but not eliminate clot formation,indicating the importance of understanding thrombosis in Mg alloys that are currently being developed for clinical use as biodegradable stents.

关键词： Vascular stents Biodegradable metals Thrombosis Contact pathway Platelets

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准规则生物组织超声背散射信号的仿真

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仪器仪表学报 2008年第9期29卷 1898-1902页

作者：黄凯他得安王威琪 Lawrence h.le 复旦大学电子工程系阿尔伯塔大学放射与诊断图像系

超声背散射信号能提供反映组织微结构的有用信息。本文在原有的生物组织超声背散射信号仿真模型基础上,将影响平均散射元间距(MSS)估计的组织特性具体为4个主要参数,为检测各种平均散射元间距估计方法的性能提供了方便。文中用AR倒谱方... 详细信息

超声背散射信号能提供反映组织微结构的有用信息。本文在原有的生物组织超声背散射信号仿真模型基础上,将影响平均散射元间距(MSS)估计的组织特性具体为4个主要参数,为检测各种平均散射元间距估计方法的性能提供了方便。文中用AR倒谱方法对仿真信号4个参数的合理性进行反向测试,实验结果表明,仿真信号的4个参数能表示影响平均散射元间距估计性能的组织特性。通过仿真信号与人体脾组织的背散射信号以及相关文献中组织信号的比较,证明仿真信号与具有准周期性的生物组织背散射信号基本类似。

关键词：超声背散射信号组织微结构仿真模型平均散射子间距(MSS)

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A review of pain outcomes following targeted muscle reinnervation in lower extremity limb pain

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Plastic and Aesthetic Research 2022年第1期9卷 65-76页

作者： Elliot L.h.le Mark A.Greyson Ryan S.Constantine Matthew L.Iorio Division of Plastic and Reconstructive Surgery University of ColoradoAnschutz Medical CenterAuroraCO 80045USA

Approximately 75%experience phantom(PLP),residual(RLP),or general(GLP)limb pain following lower extremity *** muscle reinnervation(TMR)is a peripheral nerve transfer that reroutes amputated nerves to motor endplates that can prevent or treat limb *** systematic review summarizes pain outcomes following primary and secondary treatment of lower extremity PLP,RLP,and *** literature review of three databases-PubMed,EMBASE,MEDLINE-were used for all articles related to TMR and lower extremity limb pain,querying the same keywords:“targeted muscle reinnervation”AND“pain”.Citations were then reviewed and eliminated if only upper extremities were studied or the study lacked pain *** were categorized as primary or secondary *** outcomes,including Numerical Rating Scales(NRS)and Patient-Reported Outcome Measurement Information System(PROMIS)Pain scores,were aggregated when *** studies met all inclusion and exclusion criteria after formal review for a total of 431 extremities,of which 79.1%(n=341 limbs)were lower *** primary TMR PROMIS scores for PLP and RLP were lower than amputees without primary *** NRS scores and PROMIS Pain scores in secondary TMR demonstrated improvements in PLP,RLP,and *** and Secondary TMR does prevent and improve PLP,RLP,and GLP;however,a minority of studies report quantifiable pain *** future TMR studies should include validated pain outcomes to better quantify the expected pain and quality of life improvements after lower extremity TMR.

关键词： Lower extremity amputation phantom limb pain residual limb pain targeted muscle reinnervation nerve transfer

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Numerical Study of Semidiscrete Penalty Approach for Stabilizing Boussinesq System with Localized Feedback Control

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Annals of Applied Mathematics 2024年第2期40卷 191-218页

作者： Mejdi Azaiez Kevin le Balc'h Universite de Bordeaux Bordeaux-INPI2M UMR5295F-33400TalenceFrance Inria Sorbonne UniversiteUniversite de ParisCNRSLaboratoire Jacques-Louis LionsParisFrance

We investigate the numerical approximation for stabilizing the semidiscrete linearized Boussinesq system around an unstable stationary *** is attained through internal feedback controls applied to the velocity and temperature equations,localized within an arbitrary open *** study follows the framework presented in[14],considering the continuous linearized Boussinesq *** primary objective is to explore the penalizationbased approximation of the free divergence condition in the semidiscrete case and provide a numerical validation of these results in a two-dimensional setting.

关键词： Boussinesq system penalty method stabilization feedback control

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基于简易反向滤波跟踪算法分析松质骨的微结构特征

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中国生物医学工程学报 2008年第2期27卷 218-223页

作者：黄凯他得安王威琪 le Lawrence h 复旦大学电子工程系上海200433 Department of Radiology ＆ Diagnostic Imaging University of Alberta Edmonton Alberta T6G 2B7 Canada

平均散射元间距(MSS)已被众多研究者用于分析准规则组织的微结构特征,平均骨小梁间距(MTBS)是诊断骨质疏松症的重要参数之一。本研究提出一种基于简易反向滤波跟踪(SIFT)的算法,用于估计平均骨小梁间距。对计算机仿真信号和离体牛胫骨... 详细信息

平均散射元间距(MSS)已被众多研究者用于分析准规则组织的微结构特征,平均骨小梁间距(MTBS)是诊断骨质疏松症的重要参数之一。本研究提出一种基于简易反向滤波跟踪(SIFT)的算法,用于估计平均骨小梁间距。对计算机仿真信号和离体牛胫骨松质骨的超声背散射信号分别进行分析处理。实验结果表明,SIFT算法能简单而有效地减小测量系统和传播介质特性的干扰,并且在较强噪声存在的情况下,SIFT算法比AR倒谱法的估计结果更为精确。因此,SIFT算法可用于估计平均骨小梁间距,从而反映松质骨的微结构特征。

关键词：松质骨平均骨小梁间距(MTBS) 超声背散射简易反向滤波跟踪(SIFT)算法

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Multimodal separation for ultrasonic Lamb wave using independent component analysis in the time-frequency domain

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Chinese Journal of Acoustics 2021年第4期40卷 583-596页

作者： LOU Sijia LIU Zhenli XU Kailiang TRAN Tho N.h.T. ZhOU Chang le Lawrence h. TA De'an Center for Biomedical Engineering Fudan UniversityShanghai 200433 Academy for Engineering and Technology Fudan UniversityShanghai 200433 Ningbo Marvoto Intelligent Technology Ningbo 315300 Department of Radiology and Diagnostic Imaging University of Alberta EdmontonCanada T6G2R3

Aiming at facilitating Lamb wave signal analysis and mode characterization,an ultrasonic-Lamb wave niultimodal separation method combining short-time Fourier transform(STFT)and independent component analysis(ICA)was *** signals were projected into the time-frequency domain through *** that the energy trajectory of each Lamb mode is indepenclent in the time-frequency domain,ICA method was then applied to separate tlie superposed *** group velocity curve of each mode was extracted according to its time-frequency energy trajectory,based on which the plate thickness can be further *** finite-difference time-domain(FDTD)simulated signals and experimental signals for a 1 nmi-thick steel plate were used to validate the performance of the proposed *** three fundainental Lamb modes,A0,SO and A1 modes were *** average errors of group velocity estimation in the simulation and experiment were about 1.5%and 2.0%,with that of plate thickness estimation being 0.3%and 2.0%*** simulated results indicate that,when the signal-to-noise ratio(SNR)is larger than 0 dB,the ICA method in the time-frequency domain can be used for ultrasonic Lamb wave multimodal separation,group velocity extraction and plate thickness estimation.

关键词： separation wave analysis

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