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rePROBe:Workflow for Revised Probe Assignment and Updated Probe-set Annotation in Microarrays

Genomics, Proteomics & Bioinformatics 2021年第6期19卷 1043-1049页

作者： Frieder Hadlich Henry Reyer Michael Oster Nares Trakooljul eduard muráni Siriluck Ponsuksili Klaus Wimmers Research Institute for Farm Animal Biology(FBN) Institute of Genome BiologyD-18196 DummerstorfGermany Faculty of Agricultural and environmental Sciences University of RostockD-18059 RostockGermany

Commercial and customized microarrays are valuable tools for the analysis of holistic expression patterns,but require the integration of the latest genomic *** study provides a comprehensive workflow implemented in an R package(rePROBe)to assign the entire probes and to annotate the probe sets based on up-to-date genomic and transcriptomic *** rePROBe package can be applied to available gene expression microarray platforms and addresses both public and custom *** revised probe assignment and updated probe-set annotation are applied to commercial microarrays available for different livestock species,i.e.,chicken(Gallus gallus;ChiGene-1_0-st:443,579 probes and 18,530 probe sets),pig(Sus scrofa;PorGene-1_1-st:592,005 probes and 25,779 probe sets),and cattle(Bos Taurus;BovGene-1_0-st:530,717 probes and 24,759 probe sets),as well as available for human(Homo sapiens;HuGene-1_0-st)and mouse(Mus musculus HT_MG-430_PM).Using current species-specific transcriptomic information(RefSeq,ensembl,and partially non-redundant nucleotide sequences)and genomic information,the applied workflow reveals 297,574 probes(15,689 probe sets)for chicken,384,715 probes(21,673 probe sets)for pig,363,077 probes(21,238 probe sets)for cattle,481,168 probes(23,495 probe sets)for human,and 324,942 probes(32,494 probe sets)for *** are representative of 12,641,15,758,18,046,20,167,and 16,335 unique genes that are both annotated and positioned for chicken,pig,cattle,human,and mouse,***,the workflow collects information on the number of single nucleotide polymorphisms(SNPs)within respective targeted genomic regions and thus provides a detailed basis for comprehensive analyses such as expression quantitative trait locus(eQTL)studies to identify quantitative and functional *** rePROBe R package is freely available at https://***/friederhadlich/rePROBe.

关键词： Mapping Microarray Probe assignment Probe-set annotation Genomic database

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A Semi-Field Approach to Testing Botanical Insecticides. effects of Natural and Analogues Annonaceous Acetogenins on <i>Spodoptera frugiperda</i>Smith (Lepidoptera: Noctuidae)

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Journal of Agricultural Chemistry and environment 2021年第4期10卷 458-468页

作者： José Ruiz Hidalgo María Gabriela murúa Adriana Neske Instituto de Química Orgánica Facultad de Bioquímica Química y Farmacia UNT Tucumán Argentina Instituto de Tecnologia Agroindustrial del Noroeste Argentino (ITANOA) Estación Experimental Agroindustrial Obispo Colombres (EEAOC)-CONICET Las TalitasTucumán Argentina Facultad de Ciencias Naturales e Instituto Miguel Lillo UNT Miguel Lillo San Miguel de Tucumán

Annonaceous acetogenins enclose a large number of biological activities, among which the insecticidal stands out. Spodoptera frugiperda is a pest that affects corn crops among others and has a great capacity to develo... 详细信息

Annonaceous acetogenins enclose a large number of biological activities, among which the insecticidal stands out. em>Spodoptera frugiperdaem> is a pest that affects corn crops among others and has a great capacity to develop resistance to traditional insecticides, which represents sufficient reasons for the search for new alternatives for their control. The objective of this study was to determine the appropriate concentration and screening new natural insecticides against second instar larvae of em>S. frugiperdaem>, under glasshouse conditions on em>Zea maysem> L. Natural products such as annonaceous acetogenins and some acetylated and methoxy methylated ACG derivatives and the commercial product Lambda-cyhalotrin (LC) were evaluated. The percent mortalities of em>S. frugiperda em>larvae in glasshouse conditions were recorded after 24, 48 and 72 h of treatment application. The results showed that the acetogenins tested were significantly (P erent in relation to pest mortality than untreated check. After 72 h of treatment application the highest percent mortalities were obtained with the mixture of two natural products, rolliniastatin-2 (5) (100 μg/mL) + squamocin (6) (100 μg/mL) + LC (50 μg/mL) that proved the most effective and gave (100%), while rolliniastatin-2 (5) at 100 μg/mL alone, gave lowest percent mortality (65%), followed by squamocin (6) at 100 μg/mL (55%) and LC at 50 μg/mL (30%). The acetylated and methoxy methylated ACGs derivatives caused very low mortality (25% - 35%). It is recommended the mixture as a management option of em>S. frugiperdaem> as a component of integrated pest management. The results allow us to infer that the addition of natural ACGs synergizes the insecticidal activity of the commercial product. Finding a new ecological alternative for insect control.

关键词： Annonaceous Acetogenins Biocides Spodoptera frugiperda

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ANALYSIS OF FOLDS SOUTH OF MALAKAND AND ADJOINING AReAS, NORTH PAKISTAN

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地学前缘 2000年第S1期7卷 69-70页

作者： Irshad Ahmad 1,Joseph A. DiPietro 2(1.nce in Geology, University of Peshawar Pakistan 2.Department of Geology, University of Southern Indiana, evansville,IN 47712,USA)

North of the study area in lower Swat, the rocks have been found to have undergone multiple deformations with at least four periods of folding during a single Paleogene metamorphism (DiPietro and lawr.nce, 1991). The earliest folds are composite W—SW vergent, syn\|metamorphic F 1/F 2 folds associated with the formation of the regional foliation. Late\|metamorphic, N—S trending open upright F 3 folds are associated with local foliation development, and e—W trending open F 4 folds are associated with retrograde metamorphism. These interfere with each other to produce dome/basin structures.

关键词： Paleogene metamorphism the regional foliation Malakand and adjoining areas,North Pakistan

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Promiscous female euxesta eluta derive nutrients for ovarian development by expelling and consuming ejaculates

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Insect Science 2020年第3期27卷 545-557页

作者： Solana Abraham Gisela Castillo Viviana Diaz Guido Van Nieuwenhove Gabriela murua Juan Rull Laboratorio de Investigaciones ecoetologicas de Moscas de la Fruta y sus enemigos Naturales(LIeMeN) PROIMI-BiotecnologiaCONICETTucumanArgentina Fundacion Miguel Ullo Instde EntomologiaDepto ZoologiaMiguel Lillo 251San Miguel de TucumanTucumanArgentina Facultad de Ciencias Naturales e I.M.L Universidad Nacional de TucumanSan Miguel de TucumanTucumanArgentina estacion experimental Agroindustrial Obispo Colombres(eeAOC)_ITANOA CONICeT TucumanArgentina

Seminal gifts range from important material donations to items that provide little direct benefit to ***,female silk corn flies euxesta eluta expel and consume male ejaculates immediately after *** evolution and function of this peculiar behavior are currently *** performed a series of experiments aimed to:determine if females under different dietary regimes derive nutrients or water for survival and/or reproduction from ejaculate consumption,if males suffer a fitness cost from supplying females with ejaculates,and if females prefer to mate and/or are more likely to store sperm from well fed than nutritionally stressed presumably inferior *** revealed that protein deprived *** females derive nutrients for ovarian development through consumption of ejaculates of protein fed *** seminal products affecting survival appear to be transferred in the consumed ***,ovarian development,in contrast to testes growth,occurs in detriment of *** preferred to mate with protein fed males,yet sperm retention in spermathecae was extremely rare after a single *** finding suggests that females could be exerting post copulatory control.A key question that remained to be addressed for the understanding of this puzzling and promiscuous mating system is what ecological factors or male traits drive females to retain sperm from one or several males in order to achieve and/or maximize fertilization potential.

关键词： anautogeny polyandry postcopulatory female control seminal gifts sperm storage

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夏洛克:一个悲剧性人物——重读《威尼斯商人》

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武汉大学学报（人文科学版） 2001年第4期54卷 484-487页

作者：陈俊武汉科技大学城建学院湖北武汉430070

夏洛克是莎士比亚笔下的犹太高利贷者 ,他贪婪、冷酷、毫无人性 ,一直受到人们的嘲笑和唾骂。但夏洛克之所以如此 ,其根源是社会矛盾造成的。他一面虔诚地读着圣经 ,一面拼命攒钱 ,过着清教徒的生活 ,然而他的复仇意识很强 ,但到头来落... 详细信息

夏洛克是莎士比亚笔下的犹太高利贷者 ,他贪婪、冷酷、毫无人性 ,一直受到人们的嘲笑和唾骂。但夏洛克之所以如此 ,其根源是社会矛盾造成的。他一面虔诚地读着圣经 ,一面拼命攒钱 ,过着清教徒的生活 ,然而他的复仇意识很强 ,但到头来落得人财两空。从中我们可以了解当时基督教社会的所谓“仁慈 ,公正”

关键词：基督教文学评论英国莎士比亚《威尼斯商人》悲剧性夏洛克形象人物形象复仇意识

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基于OO4O访问Oracle9iSpatial空间数据

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计算机工程与应用 2003年第33期39卷 194-196页

作者：陆丽珍刘南刘仁义浙江大学地球科学系浙江省GIS重点实验室杭州310028

GIS的数据组织在经历了CAD数据模型和Coverage数据模型两个阶段后,现已发展到对象关系数据模型阶段。该文阐述了采用对象关系模型的Oracle9iSpatial技术;分析了用VC++开发基于Oracle9iSpatial的GIS应用系统时,访问Spatial中空间数据的... 详细信息

GIS的数据组织在经历了CAD数据模型和Coverage数据模型两个阶段后,现已发展到对象关系数据模型阶段。该文阐述了采用对象关系模型的Oracle9iSpatial技术;分析了用VC++开发基于Oracle9iSpatial的GIS应用系统时,访问Spatial中空间数据的关键技术;并给出了实现代码。

关键词： Oracle9i Spatial VC++ OO4O 空间数据数据组织

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西夏文字特征提取的研究

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计算机工程与应用 2002年第13期38卷 38-41页

作者：马希荣王行愚华东理工大学信息学院上海200237 宁夏大学计算中心银川750021

字符的识别研究已取得了许多研究成果。西夏字的识别研究尚属一个待开发的新领域。西夏字识别的主要问题有:它属于表意文字,还是一个大字符集,具有极高的相似度(有许多结构相似的西夏字在西夏字符集中)。西夏文字的特征提取是整个西夏... 详细信息

字符的识别研究已取得了许多研究成果。西夏字的识别研究尚属一个待开发的新领域。西夏字识别的主要问题有:它属于表意文字,还是一个大字符集,具有极高的相似度(有许多结构相似的西夏字在西夏字符集中)。西夏文字的特征提取是整个西夏文字识别系统中最重要的一部分。该文针对西夏文字的特点,提出了能充分反映西夏文字特点的几种特征并加以提取;用这些特征可以有效地识别西夏字。

关键词：西夏文字特征提取字符识别计算机

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Cultivable yeasts associated with demosponges from Puerto Rico

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Studies in Fungi 2019年第1期4卷 153-161页

作者： Rosado-Rodríguez G Sánchez-Martínez e Maldonado-Ramírez SL Otero e University of Puerto Rico at Mayagüez Department of Marine SciencesCall Box 9000Mayagüez PR 00681 University of Puerto Rico at Mayagüez Department of BiologyCall Box 9000Mayagüez PR 00681

Sponges are known for their symbiotic associations with bacteria and to a lesser extent with *** has been argued that the association between fungi and sponges is not truly symbiotic,and fungal presence is ***,a vertically transmitted endosymbiotic yeast has been observed by transmission electron microscopy in sponges of the genus *** work was focused on documenting the presence of yeasts associated with marine sponges from Puerto *** samples were taken from healthy mature colonies of Ircinia strobilina,Tedania ignis,and Chondrilla caribensis.A total of 36 yeast morphotypes were isolated and analysed by sequencing the nuclear ribosomal ITS *** cerevisiae,was isolated from all the samples,comprising the first report of this yeast as a common inhabitant in marine *** yeasts isolated include the halophilic black yeast,Hortaea werneckii,and an unidentified species belongs to the Sporidiobolales(Basidiomycota).The high isolation frequency of *** from our sponge samples supports the possibility that Saccharomyces cerevisiae as an active member of the sponges’microbial community.

关键词： fungi Hortaea marine sponges Porifera Rhodotorula Saccharomyces cerevisiae

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数值密钥分解算法的研究与实现

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计算机工程与应用 2004年第19期40卷 78-81页

作者：刘海峰焦占亚马令坤陕西科技大学计算机与信息工程学院陕西咸阳712081 陕西科技大学电子与电气工程学院陕西咸阳712081

该文由传统的钥匙分配问题入手,从正交投影的角度论述和分析了密钥空间中的向量A(密钥)在若干不同子空间上正交投影形成密钥分存方案的算法及数量,并且用C语言实现了密钥分存方案的算法,简述了编程实现算法的技术要点。算法不仅实现了... 详细信息

该文由传统的钥匙分配问题入手,从正交投影的角度论述和分析了密钥空间中的向量A(密钥)在若干不同子空间上正交投影形成密钥分存方案的算法及数量,并且用C语言实现了密钥分存方案的算法,简述了编程实现算法的技术要点。算法不仅实现了密钥分解分存,而且对信息分解和安全分存给出了一条有效途径。

关键词：密钥子空间正交投影子密钥密钥分存方案的算法

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VXI等效采样示波器模块的设计实现

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数据采集与处理 2001年第3期16卷 358-362页

作者：雷武虎王砚方安琪中国科学技术大学近代物理系合肥230027

介绍了一个基于 VXI的等效采样示波器模块的设计原理与实现方法 ,重点阐述了基于 DSP的等效采样算法和实现方法。该模块设计为消息基 VXI模块。模块按照 VXI Plug & Play规范进行设计 ,即可以单独作为一台示波器工作 ,也可方便地与其他... 详细信息

介绍了一个基于 VXI的等效采样示波器模块的设计原理与实现方法 ,重点阐述了基于 DSP的等效采样算法和实现方法。该模块设计为消息基 VXI模块。模块按照 VXI Plug & Play规范进行设计 ,即可以单独作为一台示波器工作 ,也可方便地与其他测试模块一起集成为所需的测试平台。模块实时采样率设计为 4 0 Msps,采用基于 DSP的等效采样技术 ,最高等效采样率可以达到 10Gsps。基于 L abwindows/ CVI的系统操作软件设计。

关键词：等效采样 DSP 示波器 VXI总线数字存储示波器模块

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