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Automated Detection and Quantification of Prostate Cancer in Needle Biopsies by Digital Image Analysis

Open Journal of Pathology 2014年第3期4卷 138-150页

作者： Vamsi Parimi laurie J. Eisengart Ximing J. Yang Department of Pathology Northwestern University Chicago IL USA Pathology Core Facility Robert H. Lurie Comprehensive Cancer Center Chicago IL USA

Introduction: Triple immunohistochemical (IHC) stains including antibodies specific for alpha-methylacyl-CoA-racemase and basal cell markers have been a valuable aid in accurate identification of prostate carcinoma. However, accurate quantification of minuscule areas of prostate carcinoma in biopsy specimens can often be a challenge. Here we assessed the diagnostic value and quantitative use of automated digital image analysis on triple IHC stained prostate needle biopsies. Methods: Twelve cases of prostate needle biopsy material including 75 needle cores were stained with triple-antibody cocktail (P504S + 34βE12 + p63). Slides were digitally scanned with the APERIO digital image analyzer and evaluated with the GENIE pattern and color recognition digital image analysis that we developed. A slide with known areas of adenocarcinoma, high grade prostatic intraepithelial neoplasia (PIN), benign glands and stroma was used as a training set for the automated digital image analysis platform. Results: Among 75 needle biopsy cores, 19 (25.33%) contained adenocarcinoma by histology. Digital image analysis recognized adenocarcinoma in 95% of these needle biopsies. The average area of the needle biopsy was 7.63 mm2 and overall the average area of tumor was 0.196 mm2. The smallest area of tumor recognized by the program was 0.0022 mm2 (0.0363% of the core) and the largest was 0.62 mm2 (8.17% of the core) among needle core biopsies. False positives resulted from areas of high grade PIN with patchy basal cells. The false negative was caused by uneven AMACR staining in one area of adenocarcinoma. Digital recognition of areas of interest was improved by three successive image analysis training which increased the sensitivity and specificity from 83% and 89% to 90% and 93%, respectively. Conclusions: Digital image analysis in concert with IHC triple staining may be useful for accurate detection and quantitative analysis of small foci of prostatic adenocarcinoma. Defining methods to i

关键词： Prostatic Adenocarcinoma Needle Biopsy Alpha-Methylacyl-CoA-Racemase P504S Basal Cell Immunohistochemistry Automated Digital Image Analysis

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中文版Excel 97 轻松入门

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丛书名：当代计算机职业培训系列教程

1997年

作者：（美）（L.佩里）laurie Perry 焦爱泉等译

本书介绍了Excel 97的基本知识及基本操作技术。

ISBN: (纸本)7111057562

本书介绍了Excel 97的基本知识及基本操作技术。

关键词：电子表格系统职业教育窗口软件教材

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投资经济学

投资经济学

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1977年

作者： J.laurie Carr 赖德良译

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Remote Sensing of Aquatic Coastal Ecosystem Processes 2006

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丛书名： Remote Sensing and Digital Image Processing

2006年

作者： laurie L. Richardson Ellsworth F. LeDrew

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Life after stroke in Appalachia

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International Journal of Nursing Sciences 2017年第2期4卷 105-111页

作者： laurie Theeke A.Noelle Lucke-Wold Jennifer Mallow Patricia Horstman West Virginia University School of Nursing Health Sciences SouthPO Box 9620MorgantownWV 26506-9620United States West Virginia University HealthCare 1 Medical Center DriveMorgantownWV 26506United States

Objective: The purpose of this study was to describe the structure of meaning in the experience of surviving stroke for adults living in ***: This qualitative phenomenological study includes a sample of 6 adult survivors of ischemic stroke who were discharged from either a community or university hospital to home in the Appalachian region. Data was collected through semi-structured interviews, transcribed, and analyzed thematically by two investigators. The explicated themes were verified by the survivors as representative of their experience. Results: Five main themes emerged: 1) Frustration with new physical and functional impairment, 2) Negative emotions including anger, guilt, loneliness, and depression 3) Need for accessible support, 4) Longing for home during recovery and, 5) Stepping forward after stroke which included sub-themes of perseverance, acceptance, and retraining. Anger was described as contributing to delayed recover and emotional lability was described as a source of anger. The familiarity of home was viewed as key to reestablishing control over one's life. Survivors described how they developed perseverance to move forward and emphasized that willingness to participate in retraining led to adapting to impairments. Acceptance was described as letting go of prior expectations of self and others so one could live in the present.

关键词： qualitative establishing stroke

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Simple, Reliable Isolation, Purification and Cultivation of Murine Skeletal Muscle Microvascular Endothelial Cells

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Journal of Biomedical Science and Engineering 2020年第12期13卷 290-299页

作者： Jianjie Wang Joseph Harvey Richard Garrad Virginia Huxley laurie Erb Gary Weisman Department of Biomedical Sciences Missouri State University Springfield USA Department of Cell Biology and Physiology Washington University St. Louis USA Department of Medical Pharmacology and Physiology Dalton Cardiovascular Research Center University of Missouri Columbia USA Department of Biochemistry Life Sciences Center University of Missouri Columbia USA

Objectives: Microvascular dysfunction in skeletal muscle is involved in metabolic and vascular diseases. Microvascular endothelial cells (MEC) are poorly characterized in the progression of associated diseases in part due to lack of availability of MEC from various animal models. The objective was to provide a fast, simple, and efficient method to isolate murine MEC derived from skeletal muscle. Methods: Dissected abdominal skeletal muscles from C57BL/6J mice at 8 - 12 weeks of age were enzymatically dissociated. MEC were isolated using a modified two-step Dynabeads™-based purification method. With a combination of Dynabeads™ - Griffonia simplicifolia lectin-I and Dynabeads™ - monoclonal antibody against CD31/PECAM-1, MEC were isolated and purified twice followed by cultivation. Results: Isolated and purified cells were viable and cultured. MEC were characterized by using immunofluorescence to identify CD31/PECAM-1, an EC marker, and two specific functional assays, which include a capillary-like tube formation and the uptake of Dil-Ac-LDL. The purity of isolated cell populations from skeletal muscle microvessels, which was assessed by flow cytometry, was 88.02% ± 2.99% (n = 6). Conclusions: This method is simple, fast, and highly reproducible for isolating MEC from murine skeletal muscle. The method will enable us to obtain primary cultured MEC from various genetic or diseased murine models, contributing to insightful knowledge of diseases associated with the dysfunction of microvessels.

关键词： Microvascular Endothelial Cells Isolation Primary Cultured Skeletal Muscle Mouse

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IR和教育

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首席财务官 2014年第7期12卷 50-55页

作者： laurie Havelock

要在IR工作中获得持续成功,哪些天赋和资质最重要？这份报告研究了IRO是们如何走到今天的位置的,以及他们最看重的教育和素质。众所周知，要成为IRO，没有固定路线。事实表明，很多职业IRO来自不同的起点，他们可能拥有财务资格、在职... 详细信息

要在IR工作中获得持续成功,哪些天赋和资质最重要？这份报告研究了IRO是们如何走到今天的位置的,以及他们最看重的教育和素质。众所周知，要成为IRO，没有固定路线。事实表明，很多职业IRO来自不同的起点，他们可能拥有财务资格、在职培训或特定行业的大学学位。虽然起点不同，但总有一些技能、个性和其他更为有用的特质决定了他们能从事这一职业。

关键词：教育 IR 资质

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Development of a Real-Time PCR Approach for the Specific Detection of Prunus mahaleb

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Food and Nutrition Sciences 2016年第8期7卷 703-710页

作者： Malcolm Burns Michael Walker Timothy Wilkes laurie Hall Kirstin Gray Gavin Nixon Science and Innovation LGC Ltd. Teddington England

In 2015 a number of cumin spice products were withdrawn from the international market because of the suspected presence of almond, a known allergen from the Prunus genus. However, immunoassay results were unable to provide unequivocal evidence of the Prunus species present, because of significant cross-reactivity with other species within the Prunus genus. A novel real-time PCR assay was developed for the specific detection of Prunus mahaleb DNA, a species known to be capable of causing false positives in almond immunoassays. The assay was developed based on available DNA sequence information from the Internal Transcribed Spacer (ITS) region, and tested against representative species within the Prunus genus to ensure no cross-reactivity. Results showed that mahaleb DNA was detected in a cumin spice product subject to the earlier international recalls, which could not be unequivocally identified using immunoassay approaches alone. This short report details preliminary results from the application of this assay, and will be of interest to analytical laboratories involved in trace detection of ingredients in support of relevant food labelling legislation.

关键词： Prunus Almond Mahaleb Herbs and Spices Real-Time PCR

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上海未婚青少年性相关行为影响因素结构方程模型分析

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中国卫生统计 2011年第2期28卷 139-141页

作者：张鹏楼超华 laurie 高尔生复旦大学公共卫生学院上海市计划生育科学研究所流行病学室 200032 美国约翰霍普金斯大学彭博公共卫生学院

目的在健康促进知信行理论的基础上构建青少年性相关行为影响因素的结构方程模型,探讨其影响因素的直接作用和间接作用。方法用计算机辅助调查6299名15～24岁未婚青少年性与生殖健康知识、性相关态度和性相关行为,对调查数据进行结构方... 详细信息

目的在健康促进知信行理论的基础上构建青少年性相关行为影响因素的结构方程模型,探讨其影响因素的直接作用和间接作用。方法用计算机辅助调查6299名15～24岁未婚青少年性与生殖健康知识、性相关态度和性相关行为,对调查数据进行结构方程模型拟合,再根据模型参数检验,通过评价修正确定最终模型。结果青少年生殖避孕知识得分较低,及格率为28.6%。大约75%的男性和55%的女性接受婚前性行为。结构方程模型拟合较好,性与生殖健康知识对性相关行为的直接作用为0.2,间接作用为0.1176,总作用为0.3176。开放的性相关态度对性相关行为起促进作用(0.28)。结论加强对青少年进行正规的、全面的性健康和生殖避孕教育,促使其性态度转变,降低性相关行为风险。

关键词：青少年性相关行为结构方程模型

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亚洲三地未婚青少年避孕状况及其影响因素分析

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中国学校卫生 2011年第7期32卷 811-813页

作者：张鹏楼超华 laurie 高尔生上海市浦东新区疾病预防控制中心 200032 上海市计划生育科学研究所流行病学室美国约翰霍普金斯大学彭博公共卫生学院

目的比较亚洲三地(河内、上海和台北)青少年婚前避孕措施使用情况及其影响因素,为制定青少年生殖健康干预措施提供参考依据。方法对三地共计17 016名15～24岁未婚青少年进行Computer-assisted Self Interview(CASI)问卷调查。结果河内... 详细信息

目的比较亚洲三地(河内、上海和台北)青少年婚前避孕措施使用情况及其影响因素,为制定青少年生殖健康干预措施提供参考依据。方法对三地共计17 016名15～24岁未婚青少年进行Computer-assisted Self Interview(CASI)问卷调查。结果河内、上海、台北三地未婚青少年有性经历者分别为4.4%(272/6 204)1,2.7%(765/6 023),31.2%(1 349/4 327),首次性行为中避孕措施使用率分别为57.0%,44.8%6,8.7%,最近一次性行为中避孕措施使用率分别为27.6%,23.5%,33.2%,每次都坚持采取避孕措施的比例分别为40.0%,27.7%5,1.7%。三地未婚青少年最常采用的避孕方法相同,前3位为避孕套、体外排精和安全期,但各种方法所占比例不同。多因素分析结果显示,性别和文化程度是影响三地未婚青少年避孕措施使用率的共同因素。在河内,避孕措施采取与否男女差异最大;在上海,家庭经济状况和避孕知识得分与避孕措施使用率成正相关;在台北,居住在城市、避孕知识得分越高的青少年避孕措施使用率越高。结论应加强未婚青少年的避孕教育,尤其是对男性和文化程度较低的青少年,同时提高避孕服务的可及性和可得性。

关键词：避孕行为因素分析,统计学青少年亚洲

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