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Blood meal analysis of tabanid fly after it biting the rare Sumatran rhinoceros

Asian Pacific Journal of Tropical Biomedicine 2013年第2期3卷 95-99页

作者： Jeffrine Japning Rovie-Ryan Zainal Zahari Zainuddin Wahap Marni Abdul Hamid Ahmad laurentius n.ambu Junaidi Payne Ex-Situ Conservation Division Department of Wildlife and National Parks(DWNP)Peninsular MalaysiaMinistry of Natural Resources and Environment Malaysia(NRE) Department of Zoology Faculty of Resource Science and TechnologyUniversiti Malaysia Sarawak(UNIMAS) Borneo Rhino Alliance(BORA) Locked Bag 207388999 Kota KinabaluSabahMalaysia Institute for Tropical Biology and Conservation(ITBC) Universiti Malaysia Sabah(UMS) Sabah Wildlife Department

Objective:To demonstrate a noninvasive large mammalian genetic sampling method using blood meal obtained from a tabanid ***:Blood meal was recovered from the abdomen of an engorged tabanid fly(Haematopota sp.)which was captured immediately after biting a Sumatran rhino in *** blood was applied on to a Whatman FTAblood *** laboratory work was conducted to extract,amplify and sequence the DnA from the *** was done by sampling the hair follicles and blood samples from the rhinoceros and subjecting it to the same laboratory ***:BLAST search and constructed phylogenetic trees confirmed the blood meal samples were indeed from the ***:This method could be used in the field application to noninvasively collect genetic *** of tabanids and other haematophagous arthropods(*** and ticks)and other blood-sucking parasites(*** and worms)could also provide information on vector-borne diseases.

关键词： noninvasive DnA sampling Blood meal Tabanid fly Sumatran rhino

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The Puzzle of the La I Lines 6520.644 A and 6519.869 A

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Spectral Analysis Review 2014年第3期2卷 10-18页

作者： laurentius Windholz Bettina Gamper Przemyslaw Glowacki Jerzy Dembczyński Institut für Experimentalphysik Technische Universitat GrazGrazOsterreich Laboratory of Quantum Engineering and Metrology Faculty of Technical PhysicsPoznań University of TechnologyPoznańPoland Institute of Control and Information Engineering Faculty of Electrical EngineeringPoznań University of TechnologyPoznańPoland

The strong La line 6520.644 ? is present in a Fourier Transform Spectrum (signal to noise ratio of 240), but its wavelength in commonly used tables (e.g. [1]) is given as 6520.770 ?, while in [2] the wavelength is given as 6520.74 ?, unclassified, with the remark “h” (hazy). The line could not be classified using known energy levels of the La atom (La I) and its first ion (La II). It appears as a single broadened peak. By a combination of laser optogalvanic spectroscopy, laser-induced fluorescence and Doppler-reduced saturation spectroscopy we could introduce a new even parity La I energy level, 35449.041 cm -1, J = 13/2, with hyperfine (hf) constants A = ?8.0(5) MHz, B = 10(10) MHz. For a second, up to now unknown neighbouring La I line (wavelength 6519.869 ?) we introduced another even parity energy level involved in the formation of the line, 41207.994 cm?-1, J = 13/2, A = 91.6(5) MHz, B = 170(50) MHz. We tried also to explain why in old tables the wavelength given was so different.

关键词： Lanthanum Energy Levels Laser Spectroscopy

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Hepatitis B virus subgenotypes and basal core promoter mutations in Indonesia

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World Journal of Gastroenterology 2009年第32期15卷 4028-4036页

作者： Andi Utama Sigit Purwantomo Marlinang Diarta Siburian Rama Dhenni Rino Alvani Gani Irsan Hasan Andri Sanityoso Upik Anderiani Miskad Fardah Akil Irawan Yusuf Wenny Astuti Achwan Soewignjo Soemohardjo Syafruddin AR Lelosutan Ruswhandi Martamala Benyamin Lukito Unggul Budihusodo laurentius Adrianus Lesmana Ali Sulaiman Susan Tai Molecular Epidemiology Division Mochtar Riady Institute for NanotechnologyLippo KarawaciTangerangBanten 15810Indonesia Hepatology Division Department of Internal MedicineFaculty of MedicineUniversity of IndonesiaJakarta 10430Indonesia Center of Gastroentero-Hepatology Department of Internal MedicineFaculty of MedicineHasanuddin UniversityMakassar 90245Indonesia Department of Internal Medicine Mataram General HospitalMataram 83127Indonesia Gastroentero-Hepatology Division Department of Internal MedicineGatot Soebroto HospitalJakarta 10410Indonesia Department of Internal Medicine Siloam Hospital Lippo KarawaciTangerang Banten 15810Indonesia

AIM： To identify the distribution of hepatitis B virus （HBV） subgenotype and basal core promoter （BCP） mutations among patients with HBV-associated liver disease in Indonesia.METHODS： Patients with chronic hepatitis （CH, n =61）, liver cirrhosis （LC, n = 62）, and hepatocellular carcinoma （HCC, n = 48） were included in this study. HBV subgenotype was identified based on S or preS gene sequence, and mutations in the HBx gene including the overlapping BCP region were examined by direct sequencing.RESULTS： HBV genotype B （subgenotypes B2, B3, B4, 85 and B7） the major genotype in the samples, accounted for 75.4%, 71.0% and 75.0% of CH, LC and HCC patients, respectively, while the genotype C （subgenotypes C1, C2 and C3） was detected in 24.6%, 29.0%, and 25.0% of CH, LC, and HCC patients, respectively. Subgenotypes B3 （84.9%） and C1 （82.2%） were the main subgenotype in HBV genotype B and C, respectively. Serotype adw2 （84.9%） and adrq＋（89.4%） were the most prevalent in HBV genotype B and C, respectively. Double mutation （A1762T/G1764A） in the BCP was significantly higher in LC （59.7%） and HCC （54.2%） than in CH （19.7%）, suggesting that this mutation was associated with severity of liver disease. The T1753V was also higher in LC （46.8%）, but lower in HCC （22.9%） and CH （18.0%）, suggesting that this mutation may be an indicator of cirrhosis.COnCLUSIOn： HBV genotype B/B3 and C/C1 are the major genotypes in Indonesia. Mutations in BCP, such as A1762T/G1764A and T1753V, might have an association with manifestations of liver disease.

关键词： Basal core promoter mutation Hepatitis Bvirus Indonesia Liver disease Subgenotype

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