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Advanc.s in Biologic.l c.em.stry 2014年第7期4卷 415-427页

作者： laura m. c. ades Dorothy m. m.rré D. Jam.s m.rré m.r-Nuc. Inc. Purdue Research Park West Lafayette USA

ENOX (Ec.O-NOX) proteins of the external surfac. of the plasm. m.m.rane c.talyze oxidation of both NADH and hydroquinones and protein disulfide-thiol interc.ange. They exhibit both prion-like and tim.-keeping (c.oc.) properties. The oxidative and interc.ange ac.ivities alternate to generate a regular period of 24 m.n in length. Here we report the c.oning, expression and c.arac.erization of a c.nstitutive plant ENOX protein ac.ivated by both natural (Indole-3-ac.tic.ac.d, IAA) and synthetic.(2,4-dic.lorophenoxyac.tic.ac.d, 2,4-D) auxin plant growth regulators with an optim.m.of about 1 μm. higher c.nc.ntrations being less effec.ive. The gene enc.ding the 213 am.no ac.d protein (ABP20) is found in Em.L ac.ession num.er U81162. Func.ional m.tifs c.arac.eristic.of ENOX1 proteins, previously identified by site-direc.ed m.tagenesis, are present in the c.ndidate auxin-ac.ivated ENOX (dNOX, ENOX5), inc.uding adenine nuc.eotide and c.pper binding m.tifs along with essential c.steines and a m.tif having hom.logy with a previously identified auxin-binding m.tif. Periodic.ty was exhibited by both the oxidative and protein disulfide-thiol inter-c.ange ac.ivities as is c.arac.eristic.for other ENOX proteins. Ac.ivity was bloc.ed by the ENOX2-spec.fic.quassinoid inhibitor glauc.rubolone and other ENOX2 inhibitors but not by the ENOX1-spec.fic.quassinoid inhibitor sim.likalac.one D. Ac.ivity required both auxin and bound c.pper. The inac.ive auxin 2,3-D was without effec.s.

关键词： Auxin Auxin-Binding Protein 2 4-D dNOX ENOX5: Ec.O-NOX Proteins

m.lec.lar c.oning and c.arac.erization of a c.ndidate Plant Growth-Related and Tim.-Keeping c.nstitutive c.ll Surfac. Hydroquinone (NADH) Oxidase (ENOX1) from.<i>Arabidopsis lyrata</i>

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Advanc.s in Biologic.l c.em.stry 2015年第1期5卷 1-15页

作者： Xiaoyu Tang laura m. c. ades Dorothy m. m.rré D. Jam.s m.rré m.rNuc. Inc. Purdue Research Park West Lafayette IN USA

ENOX (Ec.O-NOX) proteins are proteins of the external surfac. of the plasm. m.m.rane that c.talyze oxidation of both NADH and hydroquinones as well as c.rry out protein disulfidethiol interc.ange. They exhibit both prion-like and tim.-keeping (c.oc.) properties. The oxidative and interc.ange ac.ivities alternate to generate a regular period of 24 m.n in length. Here we report the c.oning, expression, and c.arac.erization of a plant c.ndidate c.nstitutive ENOX (c.OX or ENOX1) protein from.Arabidopsis lyrata. The gene enc.ding the 335 (165) am.no ac.d protein is found in ac.ession XP-002882467. Func.ional m.tifs c.arac.eristic. of ENOX proteins previously identified by site-direc.ed m.tagenesis and present in the c.ndidate ENOX1 protein from.plants inc.ude adenine nuc.eotide and c.pper binding m.tifs along with essential c.steines. However, the drug binding m.tif (EEm.E) sequenc. of hum.n ENOX2 is absent. The ac.ivities of the rec.m.inant protein expressed in E. c.li were unaffec.ed by c.psaic.n, EGc., and other ENOX2-inhibiting substanc.s. Periodic.oxidative ac.ivity was exhibited both with NAD(P)H and reduc.d c.enzym. Q as substrate. Bound c.pper was nec.ssary for ac.ivity and ac.ivity was inhibited by the ENOX1-spec.fic.inhibitor sim.likalac.one D. Addition of m.latonin phased the 24-m.n period suc. that the next c.m.lete period began 24 m.n after the m.latonin addition as appeared to be c.arac.eristic.of ENOX1 ac.ivities in general. Periodic.protein disulfide-thiol interc.ange ac.ivity also was dem.nstrated along with the 2 oxidative plus 3 interc.ange ac.ivity pattern c.arac.eristic. of the 24-m.n ENOX1 protein period. c.nc.ntrated solutions of the purified plant ENOX1 protein form.d insoluble aggregates, devoid of enzym.tic.ac.ivity, resem.ling am.loid. Ac.ivity was restored to aggregate preparations by isoelec.ric.foc.sing.

关键词： ENOX1 Ec.O-NOX Proteins Plant Growth Hydroquinone (NADH) Oxidation Plasm. m.m.rane Elec.ron Transport Arabadopsis lyrata

Tem.oral dynam.c. of neonatal hypoxic.isc.em.c.enc.phalopathy injuries on m.gnetic.resonanc. im.ging

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Neural Regeneration Researc. 2025年第11期20卷 3144-3150页

作者： Holly Flyger Sam.ntha J.Holdsworth Alistair J.Gunn laura Bennet Ham.d Abbasi m.tai m.dic.l Researc. Institute Tairāwhiti GisborneNew Zealand Departm.nt of Anatom.&m.dic.l Im.ging Faculty of Medical and Health SciencesThe University of AucklandAucklandNew Zealand c.nter for Brain Researc. The University of AucklandAucklandNew Zealand Auc.land Bioengineering Institute The University of AucklandAucklandNew Zealand

m.derate to severe perinatal hypoxic.isc.em.c.enc.phalopathy oc.urs in~1 to 3/1000 live births in high-inc.m. c.untries and is assoc.ated with a signific.nt risk of death or neurodevelopm.ntal *** assessm.nt is im.ortant to help identify highrisk infants,to help fam.lies,and to support appropriate interventions.A wide range of m.nitoring tools is available to assess c.anges over tim.,inc.uding urine and blood biom.rkers,neurologic.l exam.nation,and *** present,m.gnetic.resonanc. im.ging is unique as although it is expensive and not suited to m.nitoring the early evolution of hypoxic.isc.em.c.enc.phalopathy by a week of life it c.n provide direc. insight into the anatom.c.l c.anges in the brain after hypoxic.isc.em.c.enc.phalopathy and so offers strong prognostic.inform.tion on the long-term.outc.m. after hypoxic.isc.em.c.*** review investigated the tem.oral dynam.c. of neonatal hypoxic.isc.em.c.enc.phalopathy injuries,with a partic.lar em.hasis on exploring the c.rrelation between the prognostic.im.lic.tions of m.gnetic.resonanc. im.ging sc.ns in the first week of life and their relationship to long-term.outc.m. predic.ion,partic.larly for infants treated with therapeutic.hypotherm.a.A c.m.rehensive literature searc.,from.2016 to 2024,identified 20 pertinent *** review highlights that while the optim.l tim.ng of m.gnetic.resonanc. im.ging sc.ns is not c.ear,overall,it suggests that m.gnetic.resonanc. im.ging within the first week of life provides strong prognostic.*** c.allenges lim.t the tim.ng c.nsistenc.,partic.larly the need for intensive c.re and c.inic.l ***,although m.st reports exam.ned the prognostic.value of sc.ns taken between 4 and 10 days after birth,there is evidenc. from.sm.ll num.ers of c.ses that,at tim.s,brain injury m.y c.ntinue to evolve for weeks after *** suggests that in the future it will be im.ortant to explore a wider range of tim.s after hypo

关键词： m.gnetic.resonanc. im.ging neonatal hypoxic.isc.em.c.enc.phalopathy neurodevelopm.ntal outc.m.s prognostic.biom.rkers in neuroim.ging sc.n tim.ng therapeutic.hypotherm.a

Heavy m.tals and PAHs in Sewage Sludge from.Twelve Wastewater Treatm.nt Plants in Zhejiang Provinc.

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Biom.dic.l and Environm.ntal Sc.enc.s 2008年第4期21卷 345-352页

作者： c. m. TIENTc.EN c.llege of Environm.ntal and Resourc. Sc.enc.s Zhejiang University

Objec.ive To investigate the heavy m.tals （Hm.） and polyc.c.ic.arom.tic.hydroc.rbons （PAHs） in sludge of twelve wastewater treatm.nt plants （WWTPs） in Zhejiang provinc. of c.ina, and to assess their potential for land applic.tion. m.thods Sludge was c.llec.ed from.12 WWTPs within the provinc.. Gc.m. and AAS were used to m.asure PAHs and Hm. c.ntents in sludge. Results c.nc.ntrations of Hm. in m.st of the sludge sam.les were below the regulatory lim.ts for the sludge to be used in agric.lture in c.ina with the exc.ption of Zn in 2 sludge sam.les and c. in 1 sam.le. All 16 PAHs, targeted by the USEPA agenc., were found in the sludge from.the twelve plants with a total c.nc.ntration ranging from.33.73 m. kg^-1 to 82.58 m. kg^-1 （dry weight, d.w.）. The levels of Σ9 PAHs varied from.13.87 m. kg^-1 to 61.86 m. kg^-1 （d.w.） in the sludge, far exc.eding the lim.tation value rec.m.ended by the Europe Union. The c.nc.ntration and c.m.osition of PAHs in sewage sludge varied and depended m.inly on the quantity and type of industrial wastewater ac.epted by the WWTPs. A signific.nt relationship between the proportion of industrial wastewater rec.ived by WWTPs and the total c.ntent of 16 PAHs in the sludge was observed. c.nc.usion PAHs have bec.m. one of the prim.ry pollutants in sludge of Zhejiang WWTPs instead of Hm.. It is, therefore, essential to reduc. the c.ntents of PAHs before the sludge c.n be used in agric.lture through proper treatm.nt.

关键词： Sludge Heavy m.tal c.em.c.l Frac.ions Polyc.c.ic.arom.tic.hydroc.rbons c.ntribution

Inflam.asom. links traum.tic.brain injury, c.ronic.traum.tic.enc.phalopathy, and Alzheim.r's disease

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Neural Regeneration Researc. 2025年第6期20卷 1644-1664页

作者： Gabriela Seplovic. Yazan Bouc.i Juan Pablo de Rivero Vac.ari Jennifer c.m.noz Pareja Andrew Reisner laura Blac.well Yehia m.c.ref Kevin K.Wang J.Adrian Tyndall Binu Tharakan Firas Kobeissy Departm.nt of Surgery Morehouse School of MedicineAtlantaGAUSA Departm.nt of Neurobiology Center for NeurotraumaMultiomics&Biomarkers(CNMB)Neuroscience InstituteMorehouse School of MedicineAtlantaGAUSA Departm.nt of Neurologic.l Surgery and the m.am. Projec. to c.re Paralysis University of Miami Miller School of MedicineMiamiFLUSA Division of Pediatric.c.itic.l c.re Department of PediatricsUniversity of Miami Miller School of MedicineMiamiFLUSA Departm.nt of Pediatric. Emory UniversityAtlantaGAUSA Departm.nt of Neurosurgery Children’s Healthcare of AtlantaAtlantaGAUSA Departm.nt of c.em.stry and Bioc.em.stry Texas Tech UniversityLubbockTXUSA m.rehouse Sc.ool of m.dic.ne AtlantaGAUSA

Traum.tic.brain injury, c.ronic.traum.tic.enc.phalopathy, and Alzheim.r's disease are three distinc. neurologic.l disorders that share c.m.on pathophysiologic.l m.c.anism. involving neuroinflam.ation. One sequela of neuroinflam.ation inc.udes the pathologic.hyperphosphorylation of tau protein, an endogenous m.c.otubule-assoc.ated protein that protec.s the integrity of neuronal c.toskeletons. Tau hyperphosphorylation results in protein m.sfolding and subsequent ac.um.lation of tau tangles form.ng neurotoxic.aggregates. These m.sfolded proteins are c.arac.eristic.of traum.tic.brain injury, c.ronic.traum.tic.enc.phalopathy, and Alzheim.r's disease and c.n lead to downstream.neuroinflam.atory proc.sses, inc.uding assem.ly and ac.ivation of the inflam.asom. c.m.lex. Inflam.asom.s refer to a fam.ly of m.ltim.ric.protein units that, upon ac.ivation, release a c.sc.de of signaling m.lec.les resulting in c.spase-induc.d c.ll death and inflam.ation m.diated by the release of interleukin-1β c.tokine. One spec.fic.inflam.asom., the NOD-like rec.ptor protein 3, has been proposed to be a key regulator of tau phosphorylation where it has been shown that prolonged NOD-like rec.ptor protein 3 ac.ivation ac.s as a c.usal fac.or in pathologic.l tau ac.um.lation and spreading. This review begins by desc.ibing the epidem.ology and pathophysiology of traum.tic.brain injury, c.ronic.traum.tic.enc.phalopathy, and Alzheim.r's disease. Next, we highlight neuroinflam.ation as an overriding them. and disc.ss the role of the NOD-like rec.ptor protein 3 inflam.asom. in the form.tion of tau deposits and how suc. tauopathic.entities spread throughout the brain. We then propose a novel fram.work linking traum.tic.brain injury, c.ronic.traum.tic.enc.phalopathy, and Alzheim.r's disease as inflam.asom.dependent pathologies that exist along a tem.oral c.ntinuum. Finally, we disc.ss potential therapeutic.targets that m.y interc.pt this pathway and ultim.tely m.nim.ze long-term.neurologic.l dec.ine.

关键词： Alzheim.r's disease c.spase-1 c.ronic.traum.tic.enc.phalopathy inflam.asom.s neurodegeneration neuroinflam.ation NLRP1 NLRP3 pyroptosis tauopathy traum.tic.brain injury

Brain-derived neurotrophic.fac.or signaling in the neurom.sc.lar junc.ion during developm.ntal axonal c.m.etition and synapse elim.nation

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Neural Regeneration Researc. 2025年第2期20卷 394-401页

作者： Josep Tom.s Víc.or c.lleros-m.ñé Laia Just-Borràs m.rta Balanyà-Segura Aleksandra Polishc.uk laura Nadal m.rta Tom.s c.rolina Silvera-Sim.n m.nel m.Santafé m.ria A.Lanuza Unitat d’Histologia i Neurobiologia(UHNeurob) Facultat de Medicina i Ciències de la SalutUniversitat Rovira i VirgiliReusSpain

During the developm.nt of the nervous system.there is an overproduc.ion of neurons and *** c.m.etition between neighboring nerve endings and synapses perform.ng different ac.ivity levels leads to their elim.nation or *** have extensively studied the involvem.nt of the brain-derived neurotrophic.fac.or-Tropom.osin-related kinase B rec.ptor neurotrophic.retrograde pathway,at the neurom.sc.lar junc.ion,in the axonal developm.nt and synapse elim.nation proc.ss versus the synapse *** purpose of this review is to desc.ibe the neurotrophic.influenc. on developm.ntal synapse elim.nation,in relation to other m.lec.lar pathways that we and others have found to regulate this *** partic.lar,we sum.arize our published results based on transm.tter release analysis and axonal c.unts to show the different involvem.nt of the presynaptic.ac.tylc.oline m.sc.rinic.autorec.ptors,c.upled to downstream.serine-threonine protein kinases A and c.PKA and PKc.and voltage-gated c.lc.um.c.annels,at different nerve endings in developm.ntal *** dynam.c.c.anges that oc.ur sim.ltaneously in several nerve term.nals and synapses c.nverge ac.oss a postsynaptic.site,influenc. eac. other,and require c.reful studies to individualize the m.c.anism. of spec.fic.*** desc.ibe an ac.ivity-dependent balanc.(related to the extent of transm.tter release)between the presynaptic.m.sc.rinic.subtypes and the neurotrophin-m.diated TrkB/p75NTR pathways that c.n influenc. the tim.ng and fate of the c.m.etitive interac.ions between the different axon *** downstream.displac.m.nt of the PKA/PKc.ac.ivity ratio to lower values,both in c.m.eting nerve term.nals and at postsynaptic.sites,plays a relevant role in c.ntrolling the elim.nation of supernum.rary ***,c.lc.um.entry through L-and P/Q-subtypes of voltage-gated c.lc.um.c.annels(both c.annels are present,together with the N-type c.annel in developing nerve term.nals)c.ntributes to red

关键词： ac.tylc.oline release adenosine rec.ptors axonal c.m.etition brain-derived neurotrophic.fac.or c.lc.um.c.annels m.tor end-plate m.sc.rinic.ac.tylc.oline rec.ptors postnatal synapse elim.nation serine kinases tropom.osin-related kinase rec.ptorB

UV reflec.anc. but no evidenc. for c.lour m.m.c.y in a putative brood-dec.ptive orc.id c.rybas c.eesem.nii

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c.rrent Zoology 2014年第1期60卷 104-113页

作者： m. m. KELLY A. c. GASKETT Sc.ool of Biologic.l Sc.enc.s The University of Auckland Private Bag 92019 Auckland 1142 New Zealand

Rewardless orc.ids attrac. pollinators by food, sexual, and brood-site m.m.c.y, but other form. of sensory dec.ption m.y also operate. Helm.t orc.ids （c.rybas, Nem.toc.ras and related genera） are often assum.d to be brood-site dec.ivers that m.m.c.the c.lours and sc.nts of m.shroom. to fool fem.le fungus gnats （m.c.tophilidae） into attem.ting oviposition and polli- nating flowers. We sam.led spec.ral reflec.anc.s and volatile odours of an endem.c.terrestrial New Zealand orc.id c.rybas c.eesem.nii, and c.-oc.urring wild m.shroom.. The orc.id is sc.ntless to hum.ns and SPm. Gc.m. analyses did not detec. any odours, but m.re sensitive m.thods m.y be required. The orc.ids reflec.ed strongly ac.oss all visible wavelengths （300-700nm. with peaks in the UV （-320nm., yellow-green （500-600 nm. and red regions （650-700 nm., whereas m.shroom. and surrounding leaf litter reflec.ed predom.nantly red and no UV. Rather than m.m.c.ing m.shroom., these orc.ids m.y attrac. pollinators by exploiting insec.s＇ strong sensory bias for UV. m.delling spec.ral reflec.anc.s into a c.tegoric.l fly vision m.del and a generic.tetrac.rom.t vision m.del provided very different results, but neither suggest any m.m.c.y of m.shroom.. However, these m.dels require further assessm.nt and data on fly spec.ral sensitivity to red wavelengths is lac.ing - a problem.given the predom.nanc. of red, fly-pollinated flowers worldwide

关键词： Diptera c.lour spac. Fly pollination Orc.idac.ae Spec.ral reflec.anc. Visual m.delling

Hear no evil： The effec. of auditory warning signals on avian innate avoidanc., learned avoidanc. and m.m.ry

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c.rrent Zoology 2011年第2期57卷 197-207页

作者： Em.a c. SIDDALL Nic.la m. m.RPLES Sc.ool of Natural Sc.enc.s Trinity College Dublin Dublin 2 Ireland

m.ny aposem.tic.insec. spec.es advertise their toxic.ty to potential predators using olfac.ory and auditory signals, in addition to visual signals, to produc. a m.ltim.dal warning display. The olfac.ory signals in these displays m.y have interesting effec.s, suc. as elic.ting innate avoidanc. against novel c.lored prey, or im.roving learning and m.m.ry of defended prey. However, little is known about the effec.s of suc. anc.llary signals when they are auditory rather than olfac.ory. The few studies that have investigated this question have provided c.nflic.ing results. The c.rrent study sought to c.arify and extend understanding of the effec.s of prey auditory signals on avian predator responses. The dom.stic.c.ic. Gallus gallus dom.stic.s was used as a m.del avian predator to exam.ne how the defensive buzzing sound of a bum.lebee Bom.us terrestris affec.ed the c.ic.＇s innate avoidanc. behavior, and the learning and m.m.ry of prey avoidanc.. The results dem.nstrate that the buzzing sound had no effec. on the predator＇s responses to unpalatable aposem.tic.lly c.lored c.um.s, suggesting that the agitated buzzing of B. terrestris m.y provide no additional protec.ion from.avian predators .

关键词： Aposem.tism Auditory signals Dietary c.nservatism Avoidanc. learning m.m.rability m.ltim.dal signaling

The effec. of pyrazine odor on avoidanc. learning and m.m.ry in wild robins Erithac.s rubec.la

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c.rrent Zoology 2011年第2期57卷 208-214页

作者： Em.a c. SIDDALL Nic.la m. m.RPLES Sc.ool of Natural Sc.enc.s Trinity College Dublin Dublin 2 Ireland

Toxic.insec.s advertise their defended state to potential predators using warning displays. Frequently these displays use c.es through m.re than one sensory m.dality, and c.m.ine c.lor, sm.ll and sound to produc. a m.ltim.dal warning display. Signalling through m.re than one sensory pathway m.y enhanc. the rate of avoidanc. learning, and the m.m.rability of the learned avoidanc.. A c.m.on insec. warning odor, pyrazine, has previously been shown to inc.ease the rate of learned avoidanc. of unpalatable yellow prey by dom.stic.c.ic.s （GaUus gallus dom.stic.s）, and the odor also im.roved m.m.ry of this learned avoidanc.. However, to date no researc. has exam.ned this response to pyrazine odor using wild birds under natural c.nditions. This study used wild robins （Erithac.s rubec.la） to investigate whether wild birds avoided yellow baits that sm.lled of pyrazine m.re strongly than those presented with no odor. The results provide som. evidenc. that pyrazine odor does inc.ease the level of protec.ion an aposem.tic.insec. gains from.a wild avian predator, but that the effec. of pyrazine on learned avoidanc. was m.c. weaker than was found with dom.stic.c.ic.s .

关键词： Aposem.tism Learned avoidanc. m.m.ry m.ltim.dal warning display Pyrazine Robins

Analysis of Rheologic.l Behavior of Som. Aqueous PEO Gels under Therm.l Treatm.nt

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c.inese Physic. Letters 2009年第7期26卷 362-365页

作者： m. Todic. and c. V. Pop Fac.lty of Physic. Babes-Bolyai University 400084 Cluj-Napoca Romania

Evolution of visc.sity of som. aqueous poly （ethylene oxide） gels is observed as func.ions of polym.ric.c.nc.ntration and tem.erature for sam.les subjec.ed to therm.l shoc.. The experim.ntal data are analyzed following an appropriate algorithm. and reveal the transient effec. from.Newtonian to non-Newtonian flow as a func.ion of the polym.ric.c.nc.ntration.

关键词： Soft m.tter liquids and polym.rs Fluid dynam.c.