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Ornithine aspartate effects on bacterial composition and metabolic pathways in a rat model of steatotic liver disease

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World Journal of Hepatology 2024年第5期16卷 832-842页

作者： Elisa Carolina Lange Pabulo Henrique Rampelotto Larisse Longo laura bainy rodrigues de freitas Carolina Uribe-Cruz Mario Reis Alvares-da-Silva Experimental Laboratory of Hepatology and Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Bioinformatics and Biostatistics Core Facility Universidade Federal do Rio Grande do SulPorto Alegre 91501-970Brazil Graduate Program in Biological Sciences:Pharmacology and Therapeutics Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Facultad de Ciencias de la Salud Universidad Católica de las MisionesPosadasMisiones 3300Argentina Division of Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007RSBrazil Conselho Nacional de desenvolvimento Científico e Tecnológico(CNPq)researcher Brasília 71605-001Brazil

BACKGROUND Metabolic-dysfunction associated steatotic liver disease(MASLD)is a hepatic manifestation of metabolic *** suggest ornithine aspartate(LOLA)as drug *** To analyze the influence of LOLA intake on gut microbiota using a nutritional model of *** Adult male Sprague Dawley rats were randomized into three groups:Control(10 rats fed with a standard diet),MASLD(10 rats fed with a high-fat and choline-deficient diet),and LOLA(10 rats receiving 200 mg/kg/d LOLA,after the 16th week receiving high-fat and choline-deficient diet).After 28 wk of the experiment,animals were euthanized,and feces present in the intestine were *** fecal DNA extraction,the V4 region of the 16S rRNA gene was amplified followed by sequencing in an Ion S5™*** Alpha and beta diversity metrics were comparable between MASLD and LOLA.3 OTUs were differentially abundant between MASLD and LOLA,which belong to the species Helicobacter rodentium,Parabacteroides goldsteinii,and Parabacteroides *** functional prediction provided two different metabolic profiles between MASLD and *** 9 pathways differentially abundant in MASLD are related to a change in energy source,adenosine/purine nucleotides degradation as well as guanosine and adenosine deoxyribonucleotides *** 14 pathways differentially abundant in LOLA are associated with four major metabolic functions primarily influenced by L-aspartate,including tricarboxylic acid cycle pathways,purine/guanosine nucleotides biosynthesis,pyrimidine ribonucleotides biosynthesis and salvage as well as lipid IVA *** Although LOLA had no influence on alpha and beta diversity in this nutritional model of MASLD,it was associated with changes in specific gut microbes and their related metabolic pathways.

关键词： Animal model Gut microbiota Metabolic-associated steatotic liver disease Metabolic prediction Ornithine aspartate

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Hepatocellular carcinoma staging systems: Hong Kong liver cancer vs Barcelona clinic liver cancer in a Western population

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World Journal of Hepatology 2019年第9期11卷 678-688页

作者： laura bainy rodrigues de freitas Larisse Longo deivid Santos Ivana Grivicich Mário Reis Alvares-da-Silva Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto AlegreRS 90035-003Brazil Experimental Hepatology and Gastroenterology Laboratory Center for Experimental ResearchHospital de Clínicas de Porto AlegrePorto AlegreRS 90035-003Brazil School of Medicine Universidade Federal do Rio Grande do SulPorto AlegreRS 90035-003Brazil Graduate Program in Health-Applied Cellular and Molecular Biology ULBRA.CanoasRS 92425-900Brazil department of Gastroenterology Hospital de Clínicas de Porto AlegrePorto AlegreRS 90035-003Brazil

BACKGROUND despite being the world’s most widely used system for staging and therapeutic guidance in hepatocellular carcinoma(HCC)treatment,the Barcelona clinic liver cancer(BCLC)system has limitations,especially regarding intermediate-grade(BCLC-B)*** recently proposed Hong Kong liver cancer(HKLC)staging system appears useful but requires validation in Western *** To evaluate the agreement between BCLC and HKLC staging on the management of HCC in a Western population,estimating the overall patient *** This was a retrospective study of HCC patients treated at a university hospital in southern Brazil between 2011 and ***,clinical,and laboratory data were *** staging was carried out according to the HKLC and BCLC systems to assess treatment *** survival was estimated based on the treatment proposed in each *** A total of 519 HCC patients were *** these,178(34.3%)were HKLC-I;95(18.3%)HKLC-IIA;47(9.1%)HKLC-IIB;29(5.6%)HKLC-IIIA;30(5.8%)HKLCIIIB;75(14.4%)HKLC-IV;and 65(12.5%)*** to the BCLC,25(4.9%)were BCLC-0;246(47.4%)BCLC-A;107(20.6%)BCLC-B;76(14.6%)BCLCC;and 65(12.5%)*** general agreement between the two systems was 80.0%-BCLC-0 and HKLC-I(100%);BCLC-A and HKLC-I/HKLC-II(96.7%);BCLC-B and HKLC-III(46.7%);BCLC-C and HKLC-IV(98.7%);BCLC-D and HKLC-V(41.5%).When sub-classifying BCLC-A,HKLC-IIB,HKLC-IIIA and HKLC-IIIB stages according to the up-to-7 in/out criterion,13.4,66.0,100 and 36.7%,respectively,of the cases were classified as up-to-7 *** In a Western population,the general agreement between the two systems was 80.0%,although in BCLC-B cases the agreement was low,suggesting that some individuals could be candidates for the curative treatment recommended by the *** authors suggest that the BCLC system should be routinely employed,although for BCLC-B cases it should be associated with the HKLC system.

关键词： Barcelona clinic liver cancer staging system Hepatocellular carcinoma Hong Kong liver cancer staging system

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The initial stages of Li_(2)O_(2) formation during oxygen reduction reaction in Li-O_(2) batteries:The significance of Li_(2)O_(2) in charge-transfer reactions within devices

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Journal of Energy Chemistry 2024年第1期88卷 223-231,I0006页

作者： Daniela M.Josepetti Bianca P.Sousa Simone A.J.rodrigues Renato G.freitas Gustavo Doubek Advanced Energy Storage Division LABCenter for Innovation on New EnergiesSchool of Chemical EngineeringUniversity of CampinasAv Albert Einstein 50013083-852CampinasSPBrazil Institute of Physics&department of Chemistry Laboratory of Computational MaterialsFederal University of Mato Grosso78060-900CuiabáMTBrazil

Lithium-oxygen batteries are a promising technology because they can greatly surpass the energy density of lithium-ion ***,this theoretical characteristic has not yet been converted into a real device with high *** with air contamination,metallic lithium reactivity,and complex discharge and charge reactions are the main issues for this technology.A fast and reversible oxygen reduction reaction(ORR)is crucial for good performance of secondary batteries',but the partial knowledge of its mechanisms,especially when devices are concerned,hinders further *** this perspective,the present work uses operando Raman experiments and electrochemical impedance spectroscopy(EIS)to assess the first stages of the discharge processes in porous carbon electrodes,following their changes cycle by cycle at initial operation.A growth kinetic formation of the discharge product signal(Li_(2)O_(2))was observed with operando Raman,indicating a first-order reaction and enabling an analysis by a microkinetic *** solution mechanism in the evaluated system was ascribed for an equivalent circuit with three time *** the time constant for the anode interface reveals to remain relatively constant after the first discharge,its surface seemed to be more *** model indicated that the reaction occurs at the Li_(2)O_(2) surface,decreasing the associated resistance during the initial discharge ***,the growth of Li_(2)O_(2) forms a hetero-phase between Li_(2)O_(2)/electrolyte,while creating a more compact and homogeneous on the Li_(2)O_(2)/cathode *** methodology here described thus offers a way of directly probing changes in surface chemistry evolution during cycling from a device through EIS analysis.

关键词： Li-O_(2)battery Operando Raman analysis Equivalent circuit modeling Time-constant distribution

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Recycling process and proto-kimberlite melt metasomatism in the lithosphere-asthenosphere boundary beneath the Amazonian Craton recorded by garnet xenocrysts and mantle xenoliths from the Carolina kimberlite

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Geoscience Frontiers 2022年第5期13卷 89-105页

作者： Fernanda Gervasoni Tiago Jalowitzki Marcelo Peres Rocha Ricardo Kalikowski Weska Eduardo Novais-rodrigues Rodrigo Antonio de freitas rodrigues Yannick Bussweiler Elisa Soares Rocha Barbosa Jasper Berndt Elton Luiz Dantas Valmir da Silva Souza Stephan Klemme Centro de Engenharias Universidade Federal de Pelotas(UFPel)PelotasBrazil Programa de Pós-graduação em Geociências Universidade Federal do Rio Grande do SulPorto AlegreBrazil Programa de Pós-graduação em Geologia Instituto de GeociênciasUniversidade de Brasília(UnB)BrasíliaBrazil Faculdade de Geociências Universidade Federal do Mato Grosso(UFMT)CuiabáBrazil Institut für Geologie und Mineralogie Universität zu KölnGermany Faculdade de Ciências e Tecnologia Universidade Federal de Goiás(UFG)Aparecida de GoiâniaBrazil Institut für Mineralogie Universität MünsterMünsterGermany

Here we present new data on the major and trace element compositions of silicate and oxide minerals from mantle xenoliths brought to the surface by the Carolina kimberlite,Pimenta Bueno Kimberlitic Field,which is located on the southwestern border of the Amazonian *** also present Sr-Nd isotopic data of garnet xenocrysts and whole-rocks from the Carolina *** xenoliths are mainly clinopyroxenites and *** of the clinopyroxenites were classified as GPP–PP–PKP(garnet-phlogopite peridotite,phlogopite-peridotite,phlogopite-K-richterite peridotite)suites,and two clinopyroxenites(eclogites)and two garnetites are relicts of an ancient subducted *** and pressure estimates yield 855–1102℃ and 3.6–7.0 GPa,*** are enriched in light rare earth elements(LREE)(La_(N)/Yb_(N)=5–62;Ce_(N)/Sm_(N)=1–3;where N=primitive mantle normalized values),they have high Ca/Al ratios(10–410),low to medium Ti/Eu ratios(742–2840),and low Zr/Hf ratios(13–26),which suggest they were formed by metasomatic reactions with CO_(2)-rich silicate *** with high TiO_(2)(>2.0 wt.%),Al_(2)O_(3)(>12.0 wt.%),and FeOt(5.0–13.0 wt.%)resemble those found in the groundmass of kimberlites,lamproites and ***,phlogopite with low TiO_(2)(de)and PIC(phlogopite-ilmenite-clinopyorxene)*** GPP-PP-PKP suite of xenoliths,together with the clinopyroxene and phlogopite major and trace element signatures suggests that an intense proto-kimberlite melt metasomatism occurred in the deep cratonic lithosphere beneath the Amazonian *** Sr-Nd isotopic ratios of pyrope xenocrysts(G3,G9 and G11)from the Carolina kimberlite are characterized by high ^(143)Nd/^(144)Nd(0.51287–0.51371)and eNd(+4.55 to+20.85)accompanied with enriched ^(87)Sr/^(86)Sr(0.70405–0.71098).These results suggest intera

关键词： Carolina kimberlite K-rich mantle xenoliths Ancient subducted slab Proto-kimberlite melt metasomatism

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Risk factors for suicidal behaviour in late-life depression:A systematic review

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World Journal of Psychiatry 2022年第1期12卷 187-203页

作者： Veronica Fernandez-rodrigues Yolanda Sanchez-Carro Luisa Natalia Lagunas laura Alejandra Rico-Uribe Andres Pemau Patricia Diaz-Carracedo Marina Diaz-Marsa Gonzalo Hervas Alejandro de la Torre-Luque department of Psychology Universidad Complutense de MadridMadrid 28223Spain department of Psychiatry Universidad Autonoma de MadridMadrid 28046Spain Centre for Biomedical Research in Mental Health(CIBERSAM) Madrid 28029Spain department of Legal Medicine Psychiatry and PathologyUniversidad Complutense de MadridMadrid 28046Spain of Psychology La Rioja International UniversityLogrono 26006Spain Institute of Psychiatry and Mental Health San Carlos Clinical HospitalMadrid 28040Spain

BACKGROUND Suicide is a leading cause of preventable death worldwide,with its peak of maximum incidence in later *** often puts an individual at higher risk for suicidal *** turn,depression deserves particular interest in old age due to its high prevalence and dramatic impact on health and *** To gather integrated evidence on the potential risk factors for suicide behaviour development in depressive older adults,and to examine the effects of depression treatment to tackle suicide behaviour in this *** A systematic review of empirical studies,published from 2000 onwards,was *** behaviour was addressed considering its varying forms(i.e.,wish to die,ideation,attempt,and completed suicide).RESULTS Thirty-five papers were selected for review,comprising both clinical and epidemiological *** of studies focused on suicidal ideation(60%).The studies consistently pointed out that the risk was related to depressive episode severity,psychiatric comorbidity(anxiety or substance use disorders),poorer health status,and loss of *** social support and loneliness were also associated with suicide behaviour in depressive older ***,the intervention studies showed that suicidal behaviour was a robust predictor of depression treatment *** in suicidal ideation were moderated by reductions in risk factors for suicide *** To sum up,common and age-specific risk factors seem to be involved in suicide development in depressive older adults.A major effort should be made to tackle this serious public health concern so as to promote older people to age healthily and well.

关键词： Late-life depression Suicide behaviour Disability Chronic disease Loneliness

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香椿的树木年轮宽度与超年生殖行为的相互关系:对树轮学研究的警示

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Journal of Plant Ecology 2024年第1期17卷 165-171页

作者： Monique Silva Costa Thaís Jorge de Vasconcellos Claudio Sergio Lisi Arno Fritz Neves Brandes Mario Tomazello-Filho Cátia Henriques Callado Instituto de Aplicação Fernando rodrigues da Silveira Universidade do Estado do Rio de JaneiroRua Barão de Itapagipe96.Rio Comprido 20261-005Rio de JaneiroBrazil Laboratório de Anatomia Vegetal Universidade do Estado do Rio de JaneiroRua São Francisco Xavier524Maracanã20550-013Rio de JaneiroBrazil departamento de Biologia Universidade Federal de SergipeAv.Marechal Rondon s/n-Rosa Elze.São Cristóvão 49100-000SergipeBrazil departamento de Biologia Geral Universidade Federal FluminenseRua Prof.Marcos Waldemar de Freitas ReisS/N.Gragoatá24210-201Rio de JaneiroBrazil Escola Superior de Agricultura Luiz de Queiroz Universidade de São PauloAv.Pádua Dias11.Piracicaba 13418-900São PauloBrazil

资源分配到生殖过程涉及到生态和进化因素,可能会减少植物的营养生长。这种相互关系在自然界中不容易观察到,因为有许多参数可以限制生殖结构的产生或生物量的增加。我们将香椿(Cedrela odorata L.,楝科)在里约热内卢州大西洋森林中的... 详细信息

资源分配到生殖过程涉及到生态和进化因素,可能会减少植物的营养生长。这种相互关系在自然界中不容易观察到,因为有许多参数可以限制生殖结构的产生或生物量的增加。我们将香椿(Cedrela odorata L.,楝科)在里约热内卢州大西洋森林中的树木年轮宽度与超年生殖行为相关联。总体而言,生殖结构的发育发生在湿润的年份,在生长季初期没有出现水分亏缺。然而,在这些年份,树木年轮宽度较小。这些结果可能与树木年轮宽度与当地气候之间缺乏相关性有关。因此,我们强调在径向生长研究中纳入生殖数据的重要性,以扩大对热带森林生长变异性的理解。

关键词：植物生长策略树木年轮学树木生态学生殖权衡

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Effects of perinatal protein deprivation and recovery on esophageal myenteric plexus

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World Journal of Gastroenterology 2010年第5期16卷 563-570页

作者： Flavio M Greggio Ricardo BV Fontes laura B Maifrino Patricia Castelucci Romeu rodrigues de Souza Edson A Liberti Autonomic Nervous System Laboratory (VQM) Department of Anatomy Instituto de Ciencias Biomedicas da Universidade de Sao Paulo 2415 Av Prof Lineu Prestes 05508-900 So Paulo Brazil

AIM:To evaluate effects of preand postnatal protein deprivation and postnatal recovery on the myenteric plexus of the rat esophagus. METHODS: Three groups of young Wistar rats (aged 42 d) were studied: normalfed (N42), proteindeprived (D42), and proteinrecovered (R42). The myenteric neurons of their esophagi were evaluated by histochemical reactions for nicotinamide adenine dinucleotide (NADH), nitrergic neurons (NADPH)diaphorase and acetylcholinesterase (AChE), immunohistochemical reaction for vasoactive intestinal polypeptide (VIP), and ultrastructural analysis by transmission electron ***: The cytoplasms of large and medium neurons from the N42 and R42 groups were intensely reactive for NADH. Only a few large neurons from the D42 group exhibited this aspect. NADPH detected in the D42 group exhibited low reactivity. The AChE reactivity was diffuse in neurons from the D42 and R42 groups. The density of large and small varicosities detected by immunohistochemical staining of VIP was low in ganglia from the D42 group. In many neurons from the D42 group, the double membrane of the nuclear envelope and the perinuclear cisterna were not detectable. NADH and NADPH histochemistry revealed no group differences in the prof ile of nerve cell perikarya (ranging from 200 to 400 μm2).CONCLUSION: Protein deprivation causes a delay in neuronal maturation but postnatal recovery can almost completely restore the normal morphology of myenteric neurons.

关键词： Esophagus Enteric nervous system Myenteric plexus Proteins Light microscopy Transmission electron microscopy

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Epigenetic marks are modulated by gender and time of the day in the hippocampi of adolescent rats:a preliminary study

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Neural Regeneration Research 2018年第12期13卷 2160-2163页

作者： Viviane Rostirola Elsner laura Reck Cechinel Louisiana Carolina Ferreira de Meireles Karine Bertoldi Ionara rodrigues Siqueira Programa de Pós-Graduacao em Ciências Biológicas:Fisiologia Instituto de Ciências Básicas da SaúdeUniversidade Federal do Rio Grande do Sul Programa de Pós-Graduacao em Biociências e Reabilitacao Centro Universitário Metodista-IPA Curso de Fisioterapia Centro Universitário Metodista-IPA

Although the involvement of gender in epigenetic machinery in peripheral tissues during the neonatal period has been suggested, the gender-related epigenetic profile of brain areas during the adolescent period is rarely exploited. Furthermore, the influence of time of day on hippocampal acetylation marks has been demonstrated in young adult and aged rats; however, there are no studies reporting epigenetic changes in the adolescent period. Therefore, this study aimed to investigate the effects of gender on hippocampal DNA methyltransferase 1 content and histone deacetylase（HDAC） activity of adolescent rats at different time points, specifically early morning and afternoon. Both epigenetic markers increased significantly in the hippocampi of female rats compared to the male group, an indicator of reduced transcriptional activity. In addition, HDAC activity during the early morning was higher compared to afternoon groups in both male and female rats, while DNA methyltransferase 1 content was not altered by the time of day. Our findings demonstrate that hippocampal DNA methylation and histone acetylation status can be influenced by gender during the adolescent period, while the time of the day impacts HDAC activity.

关键词： adolescent rats DNA methyltransferase 1 histone deacetylase hippocampus time of the day gender epigenetic marks

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Antioxidant Migration Studies in Chitosan Films Incorporated with Plant Extracts

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Journal of Renewable Materials 2018年第5期6卷 548-558页

作者： Victor Gomes Lauriano Souza Patricia freitas rodrigues Maria Paula Duarte Ana Luisa Fernando MEtRiCS Departamento de Ciências e Tecnologia da BiomassaFaculdade de Ciências e Tecnologia(FCT)Universidade NOVA de LisboaCampus da Caparica2829-516 CaparicaPortugal CENIMAT/I3N Departamento de Ciências dos MateriaisFaculdade de Ciências e Tecnologia(FCT)Universidade NOVA de LisboaCampus da Caparica2829-516 CaparicaPortugal

The aim of this work was to develop an active biopolymer based on chitosan by incorporating natural *** essential oils(ginger,rosemary,sage,tea tree and thyme)and six hydro-alcoholic extracts(from ginger,green and black tea,kenaf leaves,rosemary and sage plants)were *** assays were carried out to evaluate the films’activity,and total phenolic content and antioxidant activity were monitored in the simulant during *** between natural compounds and polymeric matrix was evaluated by FTIR *** diffusion of the phenolic compounds was not detected in the films incorporated with hydro-alcoholic extracts(HAEs),indicating their entrapment in the *** was observed in the films with essential oils(EOs),and biobased films incorporated with ginger,sage or rosemary essential oils,presented the highest diffusion and antioxidant activity in the simulant,highlighting their functionality and potential to be used as food active packaging material.

关键词： Active packaging antioxidant activity biopolymers chitosan phenolic content

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Characterization of porous cobalt hexacyanoferrate and activated carbon electrodes under dynamic polarization conditions in a sodium-ion pseudocapacitor

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Journal of Energy Chemistry 2021年第3期30卷 53-62页

作者： Bruno Morandi Pires Willian Goncalves Nunes Bruno Guilherme freitas Francisca Elenice rodrigues Oliveira Vera Katic Cristiane Barbieri Rodella Leonardo Morais Da Silva Hudson Zanin Advanced Energy Storage Division Manufacturing CroupCarbon Sci-Tech LabsCenter for Innovation on New EnergiesUniversity of CampinasCampinas 13083-852SPBrazil Brazilian Synchrotron Light Laboratory(LNLS/Brazilian Center of Energy and Materiab(CNPEM)) Campinas 13083-970SPBrazil department of Chemistry Laboratory of Fundamental and Applied ElectrochemistryFederal University of Jequitinhonha and Mucuri's VatleyAlto dajacuba 39100-000Diamantina-MGBrazil

We report here the activated carbon and cobalt hexacyanoferrate composite,which is applied as the electrode materials in symmetric supercapacitors containing a 1.0 M Na_(2)SO_(4) aqueous *** novel material combines high specific surface area and electrochemical stability of activated carbon with the redox properties of cobalt hexacyanoferrate,resulting in maximum specific capacitance of 329 F g^(-1) with large voltage working window of 2.0 *** studies indicated that cobalt hexacyanoferrate introduces important pseudocapacitive properties accounting for the overall charge-storage process,especially when Iderstanding the energy storage process we also provided a careful investigation of the electrode materials under dynamic polarization conditions using the in situ Raman spectroscopy and synchrotron light Xray diffraction *** complementary findings were obtained in these *** believe that this novel electrode material is promising for applications regarding the energy-storage process in pseudocapacitors with long lifespan properties.

关键词： Operando studies Raman XRD synchrotron light Cobalt hexacyanoferrate Activated carbon High specific capacitance

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