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The concentration game: differential effects of bioactive signaling in 2D and3D culture

Neural Regeneration Research 2016年第1期11卷 66-68页

作者： laura a.smith callahan The Vivian L.smith Department of Neurosurgery & Center for Stem Cell and Regenerative Medicine University of Texas Health Science Center at Houston

Traumatic injuries to the central nervous system,such as traumatic brain injury,spinal cord injury and stroke,have a high prevalence,enormous financial costs and lack clinical treatments that restore neurological function（Ma et al.,2014）These injuries trigger a series of secondary biochemical and cellular responses that ultimately lead to cellular death and themaintenance of an unsupportive extracellular matrix （ECM） for tissue regeneration （Silva et al., 2014）. Artificial ECM or scaf- folds represent a way to alter this unsupportive environment to improve the efficacy of stem cell therapies and enhance neural tissue regeneration （Figure 1）.

关键词： bioactive injuries restore biochemical neurological traumatic signaling scaffold ultimately likely

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Erratum:Rates of readmission and reoperation after operative management of midshaft clavicle fractures in adolescents

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World Journal of Orthopedics 2023年第6期14卷 502-504页

作者： laura A Carrillo Hao Hua Wu Matt callahan Aman Chopra Toshali Katyal Ishaan Swarup School of Medicine Medical College of WisconsinMilwaukeeWI 53226United States Department of Orthopaedic Surgery UCSFSan FranciscoCA 94143United States School of Medicine Georgetown UniversityWashingtonDC 20007United States

This is an erratum to an already published *** found an error in the results section and Table ***,we have revised results with n≤10 to be reflected as such,which is consistent with the reporting instructions by the Agency for Healthcare Research and *** note,these changes do not affect our results,and we had previously listed this requirement in the results *** apologize for our unintentional mistake.

关键词： Adolescent Clavicle fracture Reoperation Readmission Correction

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Rates of readmission and reoperation after operative management of midshaft clavicle fractures in adolescents

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World Journal of Orthopedics 2021年第12期12卷 1001-1007页

作者： laura A Carrillo Hao-Hua Wu Aman Chopra Matt callahan Toshali Katyal Ishaan Swarup School of Medicine Medical College of WisconsinMilwaukeeWI 53226United States Department of Orthopaedic Surgery UCSFSan FranciscoCA 94143United States School of Medicine Georgetown University School of MedicineWashington D.C.WA 20007United States

BACKGROUND The national rates of readmission and reoperation after open reduction internal fixation(ORIF)of midshaft clavicle fractures in adolescents is *** To determine rates of and risk factors for readmission and reoperation after ORIF of midshaft clavicle fractures in *** This retrospective study utilized data from the Healthcare Cost and Utilization Project State Inpatient Database for California and Florida and included 11728 patients 10–18 years of age that underwent ORIF of midshaft clavicle fracture between 2005 and *** within ninety days,reoperations within two years,and differences in patient demographic factors were determined through descriptive,univariate,and multivariate *** In total,3.29%(n=11)of patients were readmitted within 90 d to a hospital at an average of 18.91±18 d after discharge,while 15.87%(n=53)of patients underwent a reoperation within two years at an average of 209.53±151 d since the index *** most common reason for readmission was a postoperative infection(n<10).Reasons for reoperation included implant removal(n=49)at an average time of 202.39±138 d after surgery,and revision ORIF(n<10)with an average time of 297±289 d after index *** odds of reoperation were higher for females(P<0.01)and outpatients(P<0.01),while the odds of reoperation were lower for patients who underwent surgery in California(P=0.02).CONCLUSION There is a low rate of readmission and a high rate of reoperation after ORIF for midshaft clavicle fractures in *** are significant differences for reoperation based on patient sex,location,and hospital type.

关键词： Adolescent Clavicle fracture Reoperation Readmission

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Defining the‘HoneySweet’insertion event utilizing NextGen sequencing and a de novo genome assembly of plum(Prunus domestica)

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Horticulture Research 2021年第1期8卷 25-37页

作者： Ann M.callahan Tetyana N.Zhebentyayeva Jodi L.Humann Christopher A.Saski Kelsey D.Galimba laura L.Georgi Ralph Scorza Dorrie Main Christopher D.Dardick USDA-ARS Appalachian Fruit Research StationKearneysvilleWV 25430USA The Schatz Center for Tree Molecular Genetics Department of Ecosystem Science and ManagementThe Pennsylvania State UniversityUniversity ParkPA 16802USA Department of Horticulture Washington State UniversityPullmanWA 99164USA Plant and Environmental Sciences Department Clemson UniversityClemsonSC 29634USA Department of Genetics and Biochemistry Clemson UniversityClemsonSC 29634USA

HoneySweet’plum(Prunus domestica)is resistant to Plum pox potyvirus,through an RNAi-triggered *** the precise nature of the transgene insertion event has been complicated due to the hexaploid genome of *** blots previously indicated an unintended hairpin arrangement of the Plum pox potyvirus coat protein gene as well as a multicopy insertion *** confirm the transgene arrangement of the insertion event,‘HoneySweet’DNA was subjected to whole genome sequencing using Illumina short-read *** indicated two different insertion events,one containing seven partial copies flanked by putative plum DNA sequence and a second with the predicted inverted repeat of the coat protein gene driven by a double 35S promoter on each side,flanked by plum *** determine the locations of the two transgene insertions,a phased plum genome assembly was developed from the commercial plum‘Improved French’.A subset of the scaffolds(2447)that were>10 kb in length and representing,>95%of the genome were annotated and used for alignment against the‘HoneySweet’transgene *** of eight matching scaffolds spanned both insertion sites ranging from 157,704 to 654,883 bp apart,however we were unable to identify which scaffold(s)represented the actual location of the insertion sites due to potential sequence differences between the two plum ***,there was no evidence of any gene(s)being interrupted as a result of the ***,RNA-seq data verified that the insertions created no new transcriptional units and no dramatic expression changes of neighboring genes.

关键词： plum insertion Sweet

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