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Genetic Diversity and Structure of Tunisian Local Pear Germplasm as Revealed by SSR Markers

Horticultural Plant Journal 2020年第2期6卷 61-70页

作者： Rim Ouni Anna Zborowska Jasna Sehic Sarra Choulak JInaki Hormaza larisa garkava-gustavsson Messaoud Mars Research Unit on Agrobiodiversity(UR13AGR05) Higher Agronomic InstituteIRESA-University of SousseChott-Mariem 4042Tunisia Department of Plant Breeding Swedish University of Agricultural Sciences23053 AlnarpSweden Department of Plant Breeding-Balsgd Swedish University of Agricultural SciencesFjkestadsven 459SE-29194 KristianstadSweden Laboratory of Genetics Biodiversity and Bioresources Valorisation(LR11ES41)ISBMUniversity of MonastirMonastir 5000Tunisia Instituto de Hortofruticultura Subtropical Mediterránea La Mayora(IHSM-UMA-CSIC)Estaci on Experimental La MayoraAlgarrobo-CostaMálaga 29750Spain

Growing pear has a long tradition in Tunisia, and numerous local cultivars possessing an excellent adaptability and resilience potential to climatic variation are present. This large adaptability is associated with an important genetic diversity, which is threatened to *** measures have to be taken in order to properly evaluate and conserve this local material. Microsatellite markers were used to assess the level of genetic diversity among Tunisian pear germplasm, and compare it with some European varieties and wild pear species. 61 pear accessions representing eight groups(six groups from Tunisia, one from Northern Europe and another group composed of wild pear) have been genotyped using SSR markers derived from apple and pear. The pear accessions showed a significant polymorphism and 95 polymorphic alleles were found. The number of alleles per locus varied from 5 for CH04e03 locus to 14 for CH01d09 locus with an average of 9.4 alleles per ***, the mean gene diversity(H_e) per locus ranged from 0.192 to 0.752. Genetic distance values and cluster analyses revealed high genetic similarities among the Tunisian groups. Factorial correspondence analysis(FCA) categorized the accessions into three independent groups where Tunisian local accessions agglomerated together distantly from European and wild pear accessions. Additionally, UPGMA dendrogram grouped accessions into two clusters, confirmed thereafter by the Bayesian model-based Structure analysis. The results showed 16 putative triploid accessions found in the local germplasm. This study provides valuable information to develop strategies of local pear conservation and use.

关键词： Pyrus communis molecular marker genetic diversity local accession

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Molecular Characterisation Reveals the Existence of a Hybridogenous Intermediary Form between Sweet Watermelon and Cow Melon Forms of Watermelon

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American Journal of Plant Sciences 2018年第6期9卷 1245-1259页

作者： Claid Mujaju Rudo Musango larisa garkava-gustavsson Department of Research and Specialist Services Research Services Division Seed Services InstituteHarare Zimbabwe Department of Research and Specialist Services Research Services Division Genetic Resources and Biotechnology InstituteHarare Zimbabwe Department of Plant Breeding Swedish University of Agricultural Sciences Uppsala Sweden

Watermelon research in Southern Africa, has predominantly observed the clear existence of the sweet watermelon and cow melon forms of watermelon, cultivated on farm and even some occurring in the wild. Molecular characterization of 48 watermelon accessions collected from National Genebank of Zimbabwe using 9 SSR markers generated a total of 49 putative alleles. The average number of alleles detected by each primer was 5.4. Analysis of molecular variance within and among accessions of watermelons revealed that only 39% of the total variation resides between these two groups (cow-melons and sweet watermelons), 24% between accession within groups and 37% within accessions. Multivariate analyses employed provide evidence of the existence of introgression between sweet water melons and cow melons, as reflected by some accessions of cow melons, clustering into a hybridogenous group. Most of watermelon accessions within the hybridogenous group [A (II)] were collected from drier communal areas, while those accessions within the cow melon group [A (I)] are mostly from research centers. The separation of cow melons into distinct groups could be indicative of a possible formation of an isolated evolutionary unit.

关键词： Sweet Watermelon Cow Melons SSR Hybridogenous Characterization Genetic Diversity

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A texture-modified dessert with high nutritional value designed for people with dysphagia:effect of refrigeration and frozen storage

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Food Science and Human Wellness 2024年第1期13卷 462-471页

作者： María Cartagena larisa Giura Diana Ansorena Iciar Astiasaran Department of Nutrition Food Science and PhysiologyFaculty of Pharmacy and NutritionUniversidad de NavarraC/Irunlarrea s/nPamplona 31008Spain Center for Nutrition Research University of Navarrac/Irunlarrea 1Pamplona 31008Spain Instituto de Investigación Sanitaria de Navarra IDISNAPamplona 31008Spain National Centre for Food Technology and Safety(CNTA)—Technology and Knowledge for Food Sector Competitiveness NavarreCrta-Na 134-km 53San Adrian 31570Spain

Dysphagia is commonly associated with malnutrition and an increased choking *** overcome these complications,food designed for people with dysphagia should have an appropriate texture and a high nutritional *** this study,six formulations of a strawberry dessert enriched in protein(calcium caseinate)and fiber(wheat dextrin or inulin)were developed using different hydrocolloids(xanthan gum,carboxymethyl cellulose or modified starch)to provide desirable texture and *** value was calculated and total phenolic content and antioxidant activity of the samples were ***-extrusion test,rheological measurements and sensory analysis were performed in refrigerated and frozen samples to characterize their textural and viscoelastic *** high content in protein(14.7 g/100 g)and fiber(7.9-8.7 g/100 g)made possible to use the claims“high protein”and“high fiber”.Phytochemicals supplied by strawberries contributed to the antioxidant properties of the *** tangent ranged 0.28-0.35 for all the formulations,indicating a weak gel behavior,which could be considered safe to *** formulations with dextrin in combination with carboxymethyl cellulose or xanthan gum seemed to be less susceptible to structural changes during frozen *** work provides insights for the development of a nutrient-dense dessert that meets the requirements of people with dysphagia.

关键词： Malnutrition Texture-modified-food Hydrocolloids Viscoelastic properties Food texture

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Age and geochemical evolution of granite magmatism in Olkhon region from Caledonian syncollisional ore-free granite to the rare metal granite and pegmatite of Middle Paleozoic intraplate setting

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China Geology 2024年第1期7卷 63-79页

作者： Viktor Antipin Valentina Makrygina larisa Kushch Nataliya Sheptyakova Vinogradov Institute of Geochemistry SB RAS664033 IrkutskRussia

The detailed description of two granite complexes in the Olkhon subterrane is *** Early Paleozoic Sharanur complex was formed by granitization of gneisses of the Olkhon *** includes migmatites,granite-gneisses,granites and pegmatites of normal alkalinity;they belong to the type of syncollisional *** Middle Paleozoic Aya granite complex includes mother Aya massif of amazonite-bearing granites and several types of rare-metal *** have elevated alkalinity,low of Ba,Sr,and high LILE and HFSE elements *** Aya pegmatites lie in northwest cracks of stretching and associated with the rise of the territory under the influence of the North Asian *** cracks and pegmatites mark the beginning of a new intraplate geodynamic *** geochemical types are distinguished among the pegmatites of this *** are amazonite pegmatites of Li-F type with Ta mineralization and complex type pegmatite with Be-Rb-Nb-Ta and Li-F mineralization(the Ilixin vein).The Tashkiney pegmatite vein is similar to Ilixin,but lies in the gneisses of the Olkhon *** shows high concentrations of Be,Nb,Ta,as well as W,Sn,but lacks Li and F,due to a greater depth and higher temperature of the melt crystallization of this pegmatite.

关键词： Pegmatite Geochemistry Submantle plume Magmatism Caledonian collision Middle Paleozoic intraplate setting Be-Rb-Nb-Ta-Li-F mineralization Olkhon region Russia

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Prognostic and predictive role of immune microenvironment in colorectal cancer

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World Journal of Gastrointestinal Oncology 2024年第3期16卷 643-652页

作者： Olesya Kuznetsova Mikhail Fedyanin larisa Zavalishina larisa Moskvina Olga Kuznetsova Alexandra Lebedeva Alexey Tryakin Galina Kireeva Gleb Borshchev Sergei Tjulandin Ekaterina Ignatova Department of Chemotherapy Federal State Budgetary Institution(N.N.Blokhin National Medical Research Center of Oncology)of the Ministry of Health of the Russian FederationMoscow 115478Russia Department of Pathology Russian Medical Academy of Continuous Professional EducationMoscow 123242Russia OncoAtlas LCC Moscow 115478Russia Federal State Budgetary Institution“National Medical and Surgical Center named after N.I.Pirogov”of the Ministry of Health of the Russian Federation Moscow 105203Russia STOONCO:Science and Technology in Oncology Moscow 115478Russia

Colorectal cancer(CRC)represents a molecularly heterogeneous disease and one of the most frequent causes of cancer-related death *** traditional classification of CRC is based on pathomorphological and molecular character-istics of tumor cells(mucinous,ring-cell carcinomas,etc.),analysis of mechanisms of carcinogenesis involved(chromosomal instability,microsatellite instability,CpG island methylator phenotype)and mutational statuses of commonly altered genes(KRAS,NRAS,BRAF,APC,etc.),as well as expression signatures(CMS 1-4).It is also suggested that the tumor microenvironment is a key player in tumor progression and metastasis in *** to the latest data,the immune microenvironment can also be predictive of the response to immune checkpoint *** this review,we highlight how the immune environment influences CRC prognosis and sensitivity to systemic therapy.

关键词： Immunoscore Immune microenvironment Colorectal cancer Gastrointestinal cancers Predictive biomarkers Digital pathology

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A high-density, multi-parental SNP genetic map on apple validates a new mapping approach for outcrossing species

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Horticulture Research 2016年第1期3卷 30-42页

作者： Erica A Di Pierro Luca Gianfranceschi Mario Di Guardo Herma JJ Koehorst-van Putten Johannes W Kruisselbrink Sara Longhi Michela Troggio Luca Bianco Hélène Muranty Giulia Pagliarani Stefano Tartarini Thomas Letschka Lidia Lozano Luis larisa garkava-gustavsson Diego Micheletti Marco CAM Bink Roeland E Voorrips Ebrahimi Aziz Riccardo Velasco François Laurens W Eric van de Weg Department of Biosciences University of MilanMilan20133Italy Plant Breeding Wageningen University and ResearchWageningen6700AJThe Netherlands Research and Innovation Centre Edmund Mach FoundationSan Michele all’Adige38010Italy Biometris Wageningen University and ResearchWageningen6700AAThe Netherlands IRHS INRAAGROCAMPUS-OuestUniversitéd’AngersSFR 4207 QUASAVBeaucouzé49071France Department of Agricultural Sciences University of BolognaBologna40127Italy Department of Molecular Biology Laimburg Research Centre for Agriculture and ForestryOra39040Italy Department of Plant Breeding Swedish University of Agricultural SciencesAlnarp23053Sweden Hendrix Genetics Research Technology&ServicesBoxmeer 5830 ACThe Netherlands

Quantitative trait loci(QTL)mapping approaches rely on the correct ordering of molecular markers along the chromosomes,which can be obtained from genetic linkage maps or a reference genome *** apple(Malus domestica Borkh),the genome sequence v1 and v2 could not meet this need;therefore,a novel approach was devised to develop a dense genetic linkage map,providing the most reliable marker-loci order for the highest possible number of *** approach was based on four strategies:(i)the use of multiple full-sib families,(ii)the reduction of missing information through the use of HaploBlocks and alternative calling procedures for single-nucleotide polymorphism(SNP)markers,(iii)the construction of a single backcross-type data set including all families,and(iv)a two-step map generation procedure based on the sequential inclusion of *** map comprises 15417 SNP markers,clustered in 3 K HaploBlock markers spanning 1267 cM,with an average distance between adjacent markers of 0.37 cM and a maximum distance of 3.29 ***,chromosome 5 was oriented according to its homoeologous chromosome *** map was useful to improve the apple genome sequence,design the Axiom Apple 480 K SNP array and perform multifamily-based QTL *** collinearity with the genome sequences v1 and v3 are *** our knowledge,this is the shortest published SNP map in apple,while including the largest number of markers,families and *** result validates our methodology,proving its value for the construction of integrated linkage maps for any outbreeding species.

关键词： breeding linkage mapping

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全球英语框架下语言信念与二语动机的相关性实证研究

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语言教育 2024年第1期12卷 44-54页

作者：顾慧媛 larisa NIKITINA 广州商学院国际交流学院广东广州马来亚大学语言学院吉隆坡

本研究在全球英语的框架下,探索英语专业学生的英语语言信念和二语学习动机的维度,并探讨语言信念如何影响学生的二语学习动机。研究数据来自460名英语专业大学生。探索性因子分析的结果表明,学生的语言信念形成了四个不同的维度,即标... 详细信息

本研究在全球英语的框架下,探索英语专业学生的英语语言信念和二语学习动机的维度,并探讨语言信念如何影响学生的二语学习动机。研究数据来自460名英语专业大学生。探索性因子分析的结果表明,学生的语言信念形成了四个不同的维度,即标准英语信念、全球英语信念、英语教育信念及中国英语信念。学生的二语动机则确定了三个维度,即国际姿态、融合定向和工具定向。回归分析的结果表明,学生的一些信念与他们的二语动机之间存在着积极的、在统计意义上有显著意义的关系。结果显示英语教育信念对学生二语动机的三个维度影响最大。研究结果具有一定的意义。首先探索性因子分析的结果支持语言信念具有不同维度的观点,并从实证角度支持全球英语概念的有效性。从教学角度看,研究结果强调了采用全球英语教学方式的必要性。

关键词：全球英语语言信念二语学习动机国际姿态

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Brain Urea as a Potential Biomarker of Neoplasm Progression

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Journal of Biosciences and Medicines 2024年第4期12卷 1-13页

作者： larisa Mikhailovna Obukhova Elena Ivanovna Erlykina Igor Aleksandrovich Medyanik Artem Sergeevich Grishin Angelina Mikhailovna Shutova Federal State Budgetary Educational Institution of Higher Education The &#8220Privolzhsky Research Medical University&#8221 of the Ministry of Health of the Russian Federation Nizhny Novgorod Russia National Research Lobachevsky State University of Nizhny Novgorod Nizhny Novgorod Russia

Metabolic reprogramming is a key feature driving oncogenesis in cancers. Recent studies have revealed that protein metabolism is largely altered in gliomas facilitating its malignant growth. Urea is the end product of nitrogen metabolism which is mainly produced by arginase. The interdependence of arginase and other biochemical mechanisms triggered scientific research interest. This research aimed to investigate the relationships between the urea as the main parameter of protein metabolism and glioma progression. It was also the most pronounced relationship between urea and the level of the nuclear protein Ki-67 as a marker of proliferative activity and O-6-methylguanine-DNA methyltransferase (MGMT), which performs DNA repair. Postoperative material from 20 patients with gliomas of different grades of anaplasia was analyzed.

关键词： Glioma Peritumoral Zone Urea Gliomal Molecular Genetic Markers Ki-67 MGMT

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Prospective study of hepatitis B and D epidemiology and risk factors in Romania:A 10-year update

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World Journal of Hepatology 2024年第4期16卷 640-649页

作者： Speranta Iacob Liana Gheorghe Mirela Onica Laura Huiban Corina Silvia Pop Ciprian Brisc Roxana Sirli Carmen Ester Cristina Mihaela Brisc Sorina Diaconu Ion Rogoveanu larisa Sandulescu Deiana Vuletici Anca Trifan Department of Gastroenterology Carol Davila University of Medicine and PharmacyBucharest 050474Romania Department of Gastroenterology and Hepatology Fundeni Clinical InstituteBucharest 022328Romania Department of Gastroenterology Grigore T Popa University of Medicine and PharmacyIasi 700115Romania Institute of Gastroenterology and Hepatology Saint Spiridon County HospitalIasi 700111Romania Department of Gastroenterology and Medical Oncology University Emergency Clinical HospitalBucharest 050098Romania Faculty of Medicine and Pharmacy University of OradeaOradea 410087Romania Department of Gastroenterology Emergency County HospitalOradea 410169Romania Center for Advanced Research in Gastroenterology and Hepatology Department of Gastroenterology and Hepatology“Victor Babeş”University of Medicine and PharmacyTimisoara 300041Romania Department of Gastroenterology and Hepatology TimişCounty Emergency Clinical Hospital"Pius Branzeu"Timisoara 300723Romania Department of Internal Medicine II and Gastroenterology University Emergency Clinical HospitalBucharest 050098Romania Department of Gastroenterology University of Medicine and PharmacyCraiova 200349Romania Department of Cardiology Emergency County HospitalCraiova 200642Romania Department of Gastroenterology Research Center of Gastroenterology and HepatologyUniversity of Medicine and PharmacyCraiova 200349Romania Department of Gastroenterology Emergency County HospitalCraiova 200642Romania Department of Gastroenterology Faculty of MedicineGrigore T Popa University of Medicine and PharmacyIasi 700115Romania

BACKGROUND The global burden of hepatitis D virus(HDV)infection represents a major medical challenge and a public health crisis ***,there is a lack of accurate data on the epidemiology and risk factors for *** B virus(HBV)and HDV coinfection causes the most severe form of viral hepatitis,leading to a higher cumulative incidence of liver-related events compared with HBV monoinfection,including the need for liver transplantation and *** To investigate the epidemiology,natural history,risk factors and clinical management of HBV and HDV coinfection in Romanian *** This prospective study was conducted between January and July 2022 in six tertiary gastroenterology and hepatology referral centres in *** consecutive adults admitted for any gastroenterology diagnosis who were HBV-positive were *** with acute hepatitis or incomplete data were *** the 25390 individuals who presented with any type of gastroenterology diagnosis during the study period,963 met the inclusion *** for anti-HDV antibodies and HDV RNA was performed for all *** and risk factor data were collected by investigators using medical charts and patient *** data were stored in an anonymized online database during the *** The prevalence of HBV was 3.8%;among these patients,the prevalence of HBV/HDV coinfection was 33.1%.The median age of the study population was 54.0 years,and it consisted of 55.1%men.A higher prevalence of HBV/HDV coinfection was observed in patients 50–69 years *** with HBV/HDV coinfection were significantly older than those with HBV monoinfection(P=0.03).Multivariate multiple regression analysis identified female gender(P=0.0006),imprisonment(P<0.0001),older age at diagnosis(P=0.01)and sexual contact with persons with known viral hepatitis(P=0.0003)as significant risk factors for *** This study shows that HDV infection among those with HBV re

关键词： Epidemiology Hepatitis B Hepatitis D Natural history Risk factors Romania

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长江口滨海湿地有机碳循环过程及影响因素研究进展

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同济大学学报（自然科学版） 2024年第2期52卷 303-312页

作者：杨中元娄厦陈仕哲 Irina Fedorova Viktorovna Dorzhievna Radnaeva larisa Elena Nikitina 同济大学土木工程学院上海200092 同济大学长江水环境教育部重点实验室上海200092 Institute of Earth Sciences Saint Petersburg State University1990347-9 Universitetskaya EmbankmentSt PetersburgRussia Laboratory of Chemistry of Natural Systems Baikal Institute of Nature Management of Siberian Branch of the Russian Academy of SciencesUlan-Ude 670047Republic of BuryatiaRussia

滨海湿地是“蓝碳生态系统”的重要组成部分。综述了长江口滨海湿地土壤有机碳时空分布及含量、碳汇速率、有机碳横向输入输出通量及量化方法、有机碳循环定量分析模型以及有机碳储量和组分对不同影响因素所做出的动态响应规律,发现在... 详细信息

滨海湿地是“蓝碳生态系统”的重要组成部分。综述了长江口滨海湿地土壤有机碳时空分布及含量、碳汇速率、有机碳横向输入输出通量及量化方法、有机碳循环定量分析模型以及有机碳储量和组分对不同影响因素所做出的动态响应规律,发现在土壤有机碳水平空间分布上,崇西湿地>崇明东滩>九段沙>南汇潮滩;有机碳通量和浓度变化主要受到植物生物量和结构、水和土壤的理化性质、陆源输入和潮汐动力、间隙水交换以及人类活动和全球气候变化的影响。未来应加强长江口湿地土壤碳库和有机碳输运通量统一观测,准确量化各主要因素对有机碳的贡献,这对研究盐沼湿地的碳循环机理和碳汇评估具有重要意义。

关键词：长江口湿地有机碳时空分布垂向埋藏速率横向输送通量影响因素碳汇评估

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