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Significance of combined detection of LunX mRNA and tumor markers in diagnosis of lung carcinoma

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Chinese Journal of Cancer Research 2014年第1期26卷 89-94页

作者： Hui Yu Xiuying Huang Zhihua Zhu Yi Hu Wei Ou lanjun zhang Ningning Zhou Department of Thoracic Surgery Sun Yat-Sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer Medicine Department of Medical Oncology Sun Yat-Sen University Cancer CenterState Key Laboratory of Oncology in South ChinaCollaborative Innovation Center for Cancer Medicine

Objective：To evaluate the significance of combined detection of LunX mRNA,carcinoembryonic antigen （CEA）,neuron-specific enolase （NSE）,and cytokeratin 21-1 fragment （CYFRA21-1） in clinical diagnosis of lung ***：Based on the quantitative RT-PCR and chemiluminescence immunoassay,the expression levels of LunX mRNA,CEA,NSE,and CYFRA21-1 in 113 patients with lung carcinoma （case group） and 30 healthy participants （control group） were ***,the sensitivity,specificity,and accuracy of the combination detection were also ***：The positive rates of LunX mRNA in peripheral blood and CEA,NSE,and CYFRA21-1 in serum were significandy higher in case group than those in control group （x2=17.295,16.825,19.148,and 17.450; P＜0.05）.There was no statistical significance when positive rate of LunX mRNA was evaluated among different pathological types （x2=0.047,P＞0.05）.The positive rate of LunX mRNA in stage Ⅰ ＋ Ⅱ,Ⅲ,and Ⅳ had a significandy increasing tendency （x2=10.565,32.462,P＜0.05）.The positive rate of CYFRA21-1 was highest in squamous carcinoma （78.5％）,the positive rate of NSE was highest in small cell carcinoma （86.7％）,and the positive rate of CEA wag highest in lung adenocarcinoma （80.4％）.The sensitivity and accuracy of the combination detection were 91.1％ and 88.1％,***：The combined detection of LunX mRNA and tumor markers （TMs） including CEA,NSE,and CYFRA21-1 in peripheral blood is helpful to increase the diagnostic accuracy of lmg ***,it can inform the pathological typing of lung carcinoma.

关键词： Lung cancer reverse transcription-polymerase chain reaction LunX mRNA carcinoembryonic antigen (CEA) neuron-specific enolase (NSE) cytokeratin 21-1 fragment (CYFRA21-1)

First-generation EGFR tyrosine kinase inhibitor therapy in 106 patients with compound EGFR-mutated lung cancer: a single institution’s clinical practice experience

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Cancer Communications 2018年第1期38卷 556-568页

作者： Xiangyang Yu Xuewen zhang Zichen zhang Yongbin Lin Yingsheng Wen Yongqiang Chen Weidong Wang lanjun zhang State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineGuangzhou 510060GuangdongChina Department of Thoracic Surgery Sun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhou 510060GuangdongChina Department of Medical Oncology Sun Yat-sen University Cancer CenterGuangzhou 510060Guang-dongChina Department of Molecular Pathology Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongChina

Background:The antitumour efficacy of tyrosine kinase inhibitors(TKIs)in lung cancer patients with compound epidermal growth factor receptor(EGFR)mutations has not been *** study summarizes a single institutional experience of first-generation TKI therapy for lung cancers with compound EGFR ***:A total of 106 consecutive patients with tumours bearing compound EGFR mutations were identified between January 2012 and May 2016;all patients received first-generation TKI *** in exon 19 and the L858R point mutation in exon 21 were considered common mutations;T790M was considered separately because of its association with TKIs *** other mutation was defined as a rare *** were divided as follows:double common mutations(group A);common plus T790M mutations(group B);common plus rare muta-tions(group C);double rare mutations(group D);and rare plus T790M mutations(group E).A separate group of 115 consecutive patients with a single common mutation was created for comparative analysis(group F).Results:The frequency of patients with compound EGFR was 2.9%(114/3925)and their response rate to first-genera-tion TKIs was 50.9%,which was not significantly different from group F(67.0%,P=0.088).The progression-free survival(PFS)of the 106 patients receiving TKI therapy was worse than that of group F(median,9.1 vs.13.0 months,respec-tively;P<0.001).The PFS of the compound mutation group was shorter than that of the single common mutation group(median,10.1 months in group A,P=0.240;9.1 months in group B,P<0.001;9.6 months in group C,P=0.010;6.5 months in group D,P=0.048;5.4 months in group E,P=0.017).Patients with a co-occurring mutation in exon 20(excluding T790M)exhibited significantly worse PFS than the patients with other compound mutations or with a single common mutation(median,6.5 vs.9.1 vs.13.0 months,respectively,P=0.002).Conclusions:There was significant heterogeneity among the compound EGFR mutations and their respons

关键词： EGFR TKIs Compound mutations

Tumor-derived apoptotic extracellular vesicle-mediated intercellular communication promotes metastasis and stemness of lung adenocarcinoma

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Bioactive Materials 2024年第6期36卷 238-255页

作者： Xiaotian He Yiyang Ma Yingsheng Wen Rusi zhang Dechang Zhao Gongming Wang Weidong Wang Zirui Huang Guangran Guo Xuewen zhang Huayue Lin lanjun zhang State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineGuangdong Provincial Clinical Research Center for CancerSun Yat-sen University Cancer CenterGuangzhouChina Department of Thoracic Surgery Sun Yat-sen University Cancer CenterSun Yat-sen UniversityGuangzhouChina Department of Breast Surgery Sun Yat-sen University Cancer CenterSun Yat-sen UniversityGuangzhouChina Department of Anesthesiology Sun Yat-sen University Cancer CenterSun Yat-sen UniversityGuangzhouChina Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhouChina Breast Tumor Center Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhouChina

Apoptosis has long been recognized as a significant mechanism for inhibiting tumor formation,and a plethora of stimuli can induce apoptosis during the progression and treatment of ***,tumor-derived apoptotic extracellular vesicles(apoEVs)are inevitably phagocytosed by live tumor cells,promoting tumor *** the mechanism by which apoEVs regulate tumor cells is imperative for enhancing our knowledge of tumor metastasis and ***,we conducted a series of in vivo and in vitro experiments,and we report that tumor-derived apoEVs promoted lung adenocarcinoma(LUAD)metastasis,self-renewal and ***,we demonstrated that apoEVs facilitated tumor metastasis and stemness by initiating the epithelial-mesenchymal transition program and upregulating the transcription of the stem cell factor *** addition,we found that ALDH1A1,which was transported by apoEVs,activated the NF-κB signaling pathway by increasing aldehyde dehydrogenase enzyme activity in recipient tumor ***,targeting apoEVs-ALDH1A1 significantly abrogated these ***,our findings elucidate a novel mechanism of apoEV-dependent intercellular communication between apoptotic tumor cells and live tumor cells that promotes the formation of cancer stem cell-like populations,and these findings reveal that apoEVs-ALDH1A1 may be a potential therapeutic target and biomarker for LUAD metastasis and recurrence.

关键词： Lung adenocarcinoma Apoptotic extracellular vesicles Stemness Proteomics SOX2

A solid-state nanopore-based single-molecule approach for label-free characterization of plant polysaccharides

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Plant Communications 2021年第2期2卷 102-110页

作者： Yao Cai Baocai zhang Liyuan Liang Sen Wang lanjun zhang Liang Wang Hong-Liang Cui Yihua Zhou Deqiang Wang College of Instrumentation and Electrical Engineering Jilin UniversityChangchunJilin 130016China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy of Seed DesignChinese Academy of SciencesBeijing 100101China Chongqing Key Laboratory of Multi-scale Manufacturing Technology Chongqing Institute of Green and Intelligent TechnologyChinese Academy of SciencesChongqing 400714China University of the Chinese Academy of Sciences Beijing 100049China

Polysaccharides are important biomacromolecules existing in all plants,most of which are integrated into a fibrillar structure called the cell *** the absence of an effective methodology for polysaccharide analysis that arises from compositional heterogeneity and structural flexibility,our knowledge of cell wall architecture and function is greatly ***,we develop a single-molecule approach for identifying plant polysaccharides with acetylated modification *** designed a solid-state nanopore sensor supported by a free-standing SiNx membrane in fluidic *** device was able to detect cell wall polysaccharide xylans at concentrations as low as 5 ng/mL and discriminate xylans with hyperacetylated and unacetylated *** further demonstrated the capability of this method in distinguishing arabinoxylan and glucuronoxylan in monocot and dicot *** the data for categorizing polysaccharide mixtures,our study establishes a single-molecule platformfor polysaccharide analysis,opening a new avenue for understanding cell wall structures,and expanding polysaccharide applications.

关键词： solid-state nanopore single-molecule polysaccharides acetylation ion current

Preferable background filtering for next-generation sequencing analysis in non-small cell lung cancer:pericarcinomatous tissues or peripheral blood lymphocytes?

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Cancer Communications 2019年第1期39卷 314-317页

作者： Yaxiong zhang Lianpeng Chang Wenfeng Fang Yunpeng Yang lanjun zhang Shaodong Hong Huaqiang Zhou Yanfang Guan Xin Yi Li zhang State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China Department of Medical Oncology Sun Yat-sen University Cancer Center651 Dongfeng Road EastGuangzhou 510060GuangdongP.R.China Geneplus-Beijing Institute Beijing 100000P.R.China Department of Thoracic Surgery Sun Yat-sen University Cancer CenterGuangzhou 510060GuangdongP.R.China

Dear editor,Lung cancer is the leading cause of cancer-related death worldwide,with the predominant pathological type being non-small cell lung cancer(NSCLC)[1,2].Next-gener-ation sequencing(NGS)analysis is increasingly used to help clinicians select appropriate target therapies,such as epidermal growth factor receptor-tyrosine kinase inhibitors(EGFR-TKIs)for EGFR-mutant patients[3].Both pericarcinomatous tissues and peripheral blood lymphocytes are widely used as normal control for NGS ***,whether pericarcinomatous tissue is suitable for background filtering in mutation analysis remains *** to the whole-genome sequencing data from The Cancer Genome Atlas(TCGA)database,there were some genomic variations in peri-carcinomatous tissue from NSCLC patients,but no driver gene mutation was detected[4,5].Therefore,deep sequencing of pericarcinomatous and tumor tissues is necessary to confirm whether pericarcinomatous tissue harbors low-frequency mutations.

关键词： lymphocytes lung filtering

Glycan acetylation patterning control is required for plant growth and stress resistance

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Glycan acetylation patterning control is required for plant ...

2018全国植物生物学大会

作者： lanjun zhang Yihua Zhou Baocai zhang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyChinese Academy of Sciences

Acetylation is a prevalent modification on biomacromolecules,such as proteins,glycan,and some phenolic compounds,and modulates their conformation and biological activities associated with a broad spectrum of biological *** is also a universal substitution presenting on the majority of cell wall polymers,mainly consisted of *** plants and bacteria,the abundance and position of acetyl groups on cell wall polymers are restrained at a certain level and vary across developmental stages and in different ***,this modification has striking impacts on plant growth and biomass utilization and seems to function as a precise regulatory mode to fine tune biological activities of ***,distinct from protein acetylation,which underlying mechanism has been well studied,acetylation on glycan remains ***,our lab has reported a Trichome birefringence-like(TBL) protein that can specially transfer acetyl groups onto hemicellulose xylan backbone and found this addition is important for leaf blight *** importantly,a new family of carbohydrate esterases,GDSL esterases,have been identificied throught characterization of a brittle leaf sheath1(bs1) ***1 is a rice xylan *** in this protein led to impaired secondary wall patterning and abnormal growth *** is the first time to reveal that the acetyl-ester profile control involves the process of deacetylation and occurs in polysaccharide biosynthesis,which outlines a new mechanism—bidirectional regulation mode in glycan acetylation *** TBLs and GDSL esterases constitute a plethora of uncharacterized family *** studies on other GDSL and TBL members reveal varied enzymatic activities and biological functions,which highlight the importance of acetylation pattern on plant growth and stress resistance.

关键词： glycan acetylation GDSL esterase secondary wall patterning bidirectional regulation

来源： cnki会议评论

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纵隔淋巴结清扫在肺癌诊疗中的共识与争议

中国肺癌杂志 2018年第3期21卷 176-179页

作者：张兰军中山大学肿瘤防治中心胸外科广州510060

淋巴结转移是肺癌的重要转移途径,淋巴结清扫术已成为肺癌的标准术式,同时也决定着肺癌的分期、预后及治疗策略。在临床实践中肺癌淋巴结的清扫方式各有不同,从选择性的淋巴结采样到扩大的淋巴结清扫,目前各种清扫方式存在着很大的争议... 详细信息

淋巴结转移是肺癌的重要转移途径,淋巴结清扫术已成为肺癌的标准术式,同时也决定着肺癌的分期、预后及治疗策略。在临床实践中肺癌淋巴结的清扫方式各有不同,从选择性的淋巴结采样到扩大的淋巴结清扫,目前各种清扫方式存在着很大的争议,本文就目前的纵隔淋巴结清扫方式的共识及争议进行综述,为今后开展多中心临床研究提供参考。

关键词：肺肿瘤淋巴结清扫治疗

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贫困大学生焦虑水平与社会支持研究

中国心理卫生杂志 2000年第3期14卷 196-196,195页

作者：张兰君西安邮电学院 710061

对贫困大学生焦虑状况及其影响因素进行调查 ,为高校贫困大学生心理卫生工作提供科学依据。方法 :选用 1— 4年级获学校贫困补助的学生 172名为研究组 ,相对应的 16 8名非贫困生为对照组 ,采用状态—特质焦虑量表和社会支持评定量表测... 详细信息

对贫困大学生焦虑状况及其影响因素进行调查 ,为高校贫困大学生心理卫生工作提供科学依据。方法 :选用 1— 4年级获学校贫困补助的学生 172名为研究组 ,相对应的 16 8名非贫困生为对照组 ,采用状态—特质焦虑量表和社会支持评定量表测评。结果 :两组特质焦虑水平差异不显著 ,而贫困生的状态焦虑水平显著高于非贫困生 ;社会支持量表评分贫困生普遍得分高于非贫困组。结论 :安慰关心、经济支持、倾诉方式 ,朋友支持。

关键词：贫困大学生焦虑社会支持

A Study of Molecular Resection Margins for Esophageal Squamous Cell Carcinoma Using Large Pathologic Sections

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Chinese Journal of Clinical Oncology 2006年第5期3卷 315-321页

作者： Guowei Ma Xu zhang JunyeWang Qiuliang Wu Hao Long Peng Lin Jianhua Fu Richard Malthaner Meiqing Zhao lanjun zhang Zhesheng Wen Tiehua Rong State Key Laboratory of Oncology in Southern China GuangzhouGuangdong 510060 China. Department of Thoracic Surgery Cancer Center Sun Yat-Sen UniversityGuangzhou Guangdong 510060 China. Pathological Department Cancer Center Sun Yat-Sen University GuangzhouGuangdong 510060 China. Division of Thoracic Surgery and Surgical Oncology London Health Sciences Centre University of Western OntarioCanada.

OBJECTIVE It has been shown that application of molecular biological techniques to surgical margins of some cancers could predict risk of local recurrence. However, the optimal length of surgical resection with tumor-free surgical margins for esophageal squamous cell carcinoma （ES- CC） is unknown. This study was conducted to evaluate the optimal length of surgical resection for ESCC with molecularly tumor-free surgical margins marked by p53 and Ki67.METHODS Surgical specimens from 70 patients with ESCC were collected for study. The lengths of the upper margin, tumor, and lower margin of every specimen were measured during the operation. Each specimen was divided into three large pathologic sections, stained with H＆E and immunohistochemically for p53 and Ki67, and examined microscopically. The lengths of the upper and lower resection ends were measured for p53 and Ki67 positive expression. The actual surgical lengths were calculated by the principle of rational shrinkage.RESULTS All surgical margins were histologically tumor-free, while the positive rates of p53 and Ki67 were 66% and 54%. The positive rates of p53 and Ki67 in the upper resecti0n end were 17% and 20%. The mean lengths of the upper resection end showing p53 and Ki67 positive expression were 1.08±1.12 cm and 1.64±1.01 cm, and the maximum lengths were 3.73 cm and 3.26 cm. The positive,rates of p53 and Ki67 in the lower resection end were 20% and 23%. The mean lengths of the lower resection end of p53 and Ki67 with positive expression were 1.11±1.15 cm and 1.34±0.94 cm, and the maximum lengths were 3.73 cm and 3.61 cm.CONCLUSION The optimal length of surgical resection with molecularly tumor-free surgical margins of ESCC is not more than 5 cm.

关键词： esophageal cancer surgery pathology molecular biology.