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Pachytene piRNAs Instruct Massive mRNA Elimination during LateSpermiogenesis

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Pachytene piRNAs Instruct Massive mRNA Elimination during La...

Different outcome of the PIWI-APC/C interplay during spermatogenesis and tumorigenesis

中国生物化学与分子生物学会第十一次会员代表大会暨2014年全国学术会议

作者： lan-tao gou Peng Dai Jian-Hua Yang En-Duo Wang Mo-Fang Liu Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences State Key Laboratory of Biocontrol Sun Yat-sen University

Spermatogenesis in mammals is characterized by two waves of piRNA expression:one correspondsto classic piRNAs responsible for silencing retrotransponsons and the secondwave is predominantly derived from non-transposon intergenic regions in pachytene spermatocytes,but the function of these pachytene piRNAs is largely ***,we reportthe involvement of pachytene piRNAs in instructing massive mRNA elimination inmouse elongating *** demonstrate that a piRNA-induced silencing complex(pi-RISC)containing murine PIWI(MIWI)and deadenylase CAF1 is selectively assembled inelongating spermatids,which is responsible for inducing mRNA deadenylation and decayvia a mechanism that resembles the action of miRNAs in somatic *** a highly orchestratedprogram appears to take full advantage of the enormous repertoire of diversifiedtargeting capacity of pachytene piRNAs derived from non-transposon intergenic *** findings suggest that pachytene piRNAs are responsible for inactivating vastcellular programs in preparation for sperm production from elongating spermatids.

关键词： pachytene piRNAs MIWI CAF1 pi-RISC mRNA deadenylation elongatings permatids

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Different outcome of the PIWI-APC/C interplay during spermat...

The PIWI-specific insertion module helps load longer piRNAs for translational activation essential for male fertility

中国生物化学与分子生物学会第十二届全国会员代表大会暨2018年全国学术会议

作者： Shuang Zhao lan-tao gou Feng Li Jun-Yan Kang Peng Yuan Mo-Fang Liu Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences-University of Chinese Academy of Sciences

PIWI proteins are known to enlist germline-specific Piwi-interacting RNAs(piRNAs) to suppress transposable elements and protect the integrity of the genome in germ *** recent studies showed that mouse PIWI(MIWI) interacts to APC/C E3 complex and is ubiquitinated by APC/C in late spermatids(Zhao et al.,Dev Cell 2013,24:13–25).Intriguingly,piRNA loading is essential for MIWI ubiquitination by APC/*** further showed that APC/C-mediated MIWI degradation releases RNF8,a histone E3 ligase,into the nucleus to mediate histone ubiquitination and subsequent histone-to-protamine exchange in late spermatids,while ubiquitination-deficient mutations in human Piwi(Hiwi) cause male infertility(gou et al.,Cell 2017,169:1090-104).Interestingly,we found that the APC/C co-activator signature elements,i.e.C-box element and IR tail,are conserved in mammalian PIWI,and that both MIWI and HIWI can activate APC/C in vitro and in ***,Miwi C-box mutant male mice showed no detectable defects in fertility,suggesting that MIWI-mediated APC/C activation is not essential for *** note,HIWI is ectopically expressed in various types of human cancers,including pancreatic ductal adenocarcinomas(PDAC).Our data showed that HIWI acts as an oncoprotein in PDAC cells,whereas HIWI-mediated APC/C activation is required for HIWI in promoting PDAC metastasis but not tumor ***,ubiquitination and degradation of PNN,a cell adhesion-related molecule,by APC/CHIWI is largely responsible for HIWI in promoting PDAC *** together,our findings suggest that piRNA loading determines the outcome of PIWI-APC/C ***-loaded PIWI in late spermatids is subjected to ubiquitination and degradation by APC/C,while apo-PIWI without piRNAs in cancer cells functions as a co-activator to promote the degradation of a class of proteins by APC/C-ubiquitin pathway.

关键词： PIWI MIWI HIWI APC/C spermatogenesis tumorigenesis

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Science China(Life Sciences) 2023年第7期66卷 1459-1481页

作者： Xin Wang Di-Hang Lin Yue Yan An-Hui Wang Jiaoyang Liao Qian Meng Wen-Qing Yang Heng Zuo Min-Min Hua Fengjuan Zhang Hongwen Zhu Hu Zhou Tian-Yu Huang Rui He Guangyong Li Yue-Qiu Tan Hui-Juan Shi lan-tao gou Dangsheng Li Ligang Wu Yonggang Zheng Xiang-Dong Fu Jinsong Li Rujuan Liu Guo-Hui Li Mo-Fang Liu Key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou310024China State Key Laboratory of Molecular Biology State Key Laboratory of Cell BiologyShanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai200031China Laboratory of Molecular Modeling and Design State Key Laboratory of Molecular Reaction DynamicsDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai201203China School of Life Science and Technology ShanghaiTech UniversityShanghai201210China NHC Key Lab of Reproduction Regulation(Shanghai Institute for Biomedical and Pharmaceutical Technologies) Fudan UniversityShanghai200032China Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education School of Basic Medical SciencesNingxia Medical UniversityYinchuan750004China Department of Urology General Hospital of Ningxia Medical UniversityNingxia Medical UniversityYinchuan750004China Institute of Reproductive and Stem Cell Engineering College of Basic of MedicineCentral South UniversityChangsha410000China Department of Physiology Howard Hughes Medical InstituteUniversity of Texas Southwestern Medical CenterDallasTX75390USA Westlake Laboratory of Life Sciences and Biomedicine School of Life SciencesWestlake UniversityHangzhou310024China

PIWI-clade proteins harness pi RNAs of 24–33 nt in *** great puzzles are how PIWI-clade proteins incorporate pi RNAs of different sizes and whether the size matters to PIWI/pi RNA *** we report that a PIWI-Ins module unique in PIWIclade proteins helps define the length of pi *** of PIWI-Ins in Miwi shifts MIWI to load with shorter pi RNAs and causes spermiogenic failure in mice,demonstrating the functional importance of this regulatory ***,we show that longer pi RNAs provide additional complementarity to target m RNAs,thereby enhancing the assembly of the MIWI/e IF3f/Hu R super-complex for translational ***,we identify a c.1108C>T(p.R370W)mutation of HIWI(human PIWIL1)in infertile men and demonstrate in Miwi knock-in mice that this genetic mutation impairs male fertility by altering the property of PIWI-Ins in selecting longer pi *** findings reveal a critical role of PIWI-Ins-ensured longer pi RNAs in fine-tuning MIWI/pi RNA targeting capacity,proven essential for spermatid development and male fertility.

关键词： PIWI piRNAs PIWI-Ins translational activation spermatid development male fertility

Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice

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Asian Journal of Andrology 2020年第6期22卷 590-601页

作者： Chen Chu Lu Yu Joelle Henry-Berger Yan-Fei Ru Ayhan Kocer Alexandre Champroux Zhi-Tong Li Miao He Sheng-Song Xie Wu-Bin Ma Min-Jie Ni Zi-Mei Ni Yun-Li Guo Zhao-Liang Fei lan-tao gou Qiang Liu Samanta Sharma Yu Zhou Mo-Fang Liu Charlie Degui Chen Andrew L Eamens Brett Nixon Yu-Chuan Zhou Joel R Drevet Yong-Lian Zhang State Key Laboratory of Molecular Biology Shanghai Key Laboratory of Molecular AndrologyCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China Genetics Reproduction and Development Laboratory CNRS UMR 6293-INSERM U1103-Universite Clermont AuvergneClermont-Ferrand 63001France Institute of Neuroscience State Key Laboratory of NeuroscieneeShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Key Laboratory of Agricultural Animal Genetics Breedingand Reproduction of the Ministry of Education and Key Laboratory of Swine Genetics and Breeding of the Ministry of AgricultureHuazhong Agricultural UniversityWuhan 430070China Department of Cellular and Molecular Medicine University of CaliforniaSan DiegoLa JollaCA 92093-0651USA Department of Anatomy Histology and EmbryologyShanghai Jiao long University School of MedicineShanghai Key Laboratory of Reproductive MedicineShanghai 200025China department of Cancer Biology Dana-Farber Cancer InstituteBostonMA 02215USA Department of Genetics Harvard Medical SchoolBostonMA 02115USA Priority Research Centre for Reproductive Sciences School of Environmental and Life SciencesThe University of NewcastleCallaghanNSW 2308Australia.

The mammalian epididymis not only plays a fun dame ntal role in the maturati on of spermatozoa,but also provides protecti on agai nst various *** foremost among these is the threat posed by oxidative stress,which arises from an imbalance in reactive oxygen species and can elicit damage to cellular lipids,proteins,and nucleic *** mice,the risk of oxidative damage to spermatozoa is mitigated through the expression and secretion of glutathione peroxidase 5(GPX5)as a major luminal scavenger in the proximal caput epididymidal ***,the loss of GPX5^-/-mediated protection leads to impaired DNA integrity in the spermatozoa of aged Gpx57 *** explore the underlying mechanism,we have conducted transcriptomic analysis of caput epididymidal epithelial cells from aged(13 months old)Gpx5^-/-m *** analysis revealed the dysregulation of several thousand epididymal mRNA transcripts,in eluding the downregulation of a subgroup of piRNA pathway gen es,in aged Gpx5^-/-*** agreeme nt with these fin dings,we also observed the loss of piRNAs,which potentially bind to the P-element-induced wimpy testis(PlWI)-like proteins PIWIL1 and *** absence of these piRNAs was correlated with the elevated mRNA levels of their putative gene targets in the caput epididymidis of Gpx5^-/-***,the oxidative stress response genes tend to have more targeting piRNAs,and many of them were among the top increased genes upon the loss of GPX5^-/-.Taken together,our findings suggest the existence of a previously uncharacterized somatic piRNA pathway in the mammalian epididymis and its possible invoIvement in the aging and oxidative stress-mediated responses.

关键词： epididymis GPX5 oxidative stress piRNA PIWI-interacting RNA small noncoding RNA

Sine oculis homeobox homolog family function in gastrointestinal cancer:Progression and comprehensive analysis

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World Journal of Clinical Oncology 2025年第1期16卷 10-24页

作者： Yang-Zheng lan Zheng Wu Wen-Jia Chen Xin-Ning Yu Hua-tao Wu Jing Liu Department of The Breast Center Cancer Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina Department of General Surgery First Affiliated Hospital of Shantou University Medical CollegeShantou 515041Guangdong ProvinceChina

The sine oculis homeobox homolog(SIX)family,a group of transcription factors characterized by a conserved DNA-binding homology domain,plays a critical role in orchestrating embryonic development and organogenesis across various organisms,including *** six distinct members,from SIX1 to SIX6,each member contributes uniquely to the development and differentiation of diverse tissues and organs,underscoring the versatility of the SIX *** or mutations in SIX genes have been implicated in a spectrum of developmental disorders,as well as in tumor initiation and progression,highlighting their pivotal role in maintaining normal developmental trajectories and cellular *** to target the transcriptional complex of the SIX gene family have emerged as a promising strategy to inhibit tumor *** the development of inhibitors targeting this gene family is still in its early stages,the significant potential of such interventions holds promise for future therapeutic ***,this review aimed to comprehensively explore the advancements in understanding the SIX family within gastrointestinal cancers,focusing on its critical role in normal organ development and its implications in gastrointestinal cancers,including gastric,pancreatic,colorectal cancer,and hepatocellular *** conclusion,this review deepened the understanding of the functional roles of the SIX family and explored the potential of utilizing this gene family for the diagnosis,prognosis,and treatment of gastrointestinal cancers.

关键词： Sine oculis homeobox homolog Gastrointestinal cancer Transcription factor Development Regulation Diagnosis Therapeutics

Luminescent Properties of Terbium（Ⅲ） and Europium（Ⅲ）Complexes with Six 4-Acetyl-bispyrazolones

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Chemical Research in Chinese Universities 2014年第2期30卷 190-193页

作者： gou Ruhu WANG Yaling YANG Rudong lan Yan Department of Chemistry and Life Science Gansu Normal University for Nationalities Hezuo 747000 P.R.China College of Chemistry and Chemical Engineering Lanzhou University Lanzhou 730000 P.R.China

The lowest triplet energy levels of the six ligands（T） were determined to be 22989 cm-1[1,3-bis-（1＇-phenyl-3＇-methyl-5＇-pyrazolon-4＇）-1,3-propanedione,BPMPTD],23148 cm-1[1,4-bis-（1＇-phenyl-3＇-methyl-5＇-pyrazolon-4＇）-1,4-butanedione,BPMPBD],23419 cm-1[1,5-bis-（1＇-phenyl-3＇-methyl-5＇-pyrazolon-4＇）-1,5-pentane-dione,BPMPPD],23310 cm-1[1,6-bis-（1＇-phenyl-3＇-methyl-5＇-pyrazolon-4＇）-1,6-hexanedione,BPMPHD],21978 cm-1[1,9-bis-（1＇-phenyl-3＇-methyl-5＇-pyrazolon-4＇）-l,9-nonanedione,BPMPND] and 21930 cm-1[1,10-bis-（1＇-phenyl-3＇-methyl-5＇-pyrazoion-4＇）-1,10-decanedione,BPMPDD],*** was explained satisfactorily that the six ligands are more efficient for sensitizing the luminescence of Tb3＋ than that of Eu3＋ at room temperature,and the order of the luminescent intensities for the Tb3＋ complexes is explained by the relative energy gap between T and 5DJ of Tb3＋ or Eu3＋.As a conclusion,when 2700 cm-1＜△E（T-5D4）＜3000 cm-1,the luminescent intensity of the Tb3＋ complex is the *** means that the lowest triplet energy level of the ligand is a chief factor to dominate RE3＋luminescence.

关键词： Rare earth complex Optical property Luminescence

Transient Performance Improvement in Tracking Control for a Class of Nonlinear Systems with Input Saturation

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Journal of Systems Science & Complexity 2018年第1期31卷 200-214页

作者： LU tao lan Weiyao LI Zhibin Department of Automation Xiamen University Xiamen 361005 China Academy of Opto-Electronics Chinese Academy of Sciences Beijing 100094 China

This paper addresses the composite nonlinear feedback（CNF） control for a class of singleinput single-output nonlinear systems with input saturation to track a time varying reference target with good transient performance. The CNF control law consists of a tracking control law and a performance compensator. The tracking control law is designed to drive the output of the system to track the time varying reference target rapidly, while the performance compensator is used to reduce the overshoot caused by the tracking control law. The stability of the closed-loop system is established. The design procedure and the improvement of transient performance of the closed-loop system are illustrated with a numerical example and the controlled Van del Pol oscillator.

关键词： Composite nonlinear feedback input saturation nonlinear systems tracking control

GSM Co-Channel and Adjacent Channel Interference Analysis and Optimization

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Tsinghua Science and Technology 2011年第6期16卷 583-588页

作者： Lina lan Xuerong gou Jingli Mao Wenyuan Ke School of Network Education Beijing University of Posts and Telecommunications Beijing 100088 China

Most current Global System for Mobile Communications （GSM） frequency planning methods evaluate the interference and assign frequencies based on measurement reports. Assigning the same or adjacent frequencies to cells close to each other will introduce co-channel and adjacent channel interference which will reduce network performance. Traditionally, man power is used to check and allocate new frequencies which is time consuming and the accuracy is not satisfactory. This paper presents an intelligent analysis method for optimization of co-channel and adjacent channel interference by exploiting cell configuration information. The method defines an interference evaluation model by analyzing various factors such as the base station layer, the azimuth ward relationship, and the cell neighborhood relationships. The interference for each frequency is evaluated and the problem frequencies are optimized. This method is verified by a large number of actual datasets from an in-service GSM network. The results show this method has better intelligence, accuracy, timeliness, and visualization than traditional methods.

关键词： GSM frequency optimization co-channel and adjacent channel interference interference evaluation model base station site layer azimuth ward neighborhood relationship triangulation

Effect of heat treatment on microstructure and mechanical properties of Ti-containing low alloy martensitic wear-resistant steel

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China Foundry 2023年第4期20卷 329-338页

作者： Kai lan Wang Ding Yi-tao Yang School of Materials Science and Engineering Shanghai UniversityShanghai 200444China

Effects of quenching temperature and cooling conditions(water cooling and 10%NaCl cooling)on microstructure and mechanical properties of a 0.2%Ti low alloy martensitic wear-resistant steel used for die casting ejector plate were *** results show that lath martensite can be obtained after austenitizing in the range of 860-980℃and then water *** an increase in austenitizing temperature,the precipitate content gradually *** precipitates are mainly composed of TiC and Ti4C2S2,and their total content is between 1.15wt.%and 1.64wt.%.The precipitate phase concentration by water-cooling is higher than that by10%NaCl cooling due to the lower cooling rate of water *** the austeniting temperature increases,the hardness and tensile strength of both water cooled and 10%NaCl cooled steels firstly increase and then *** experimental steel exhibits the best comprehensive mechanical properties after being austenitized at 900℃,cooled by 10%NaCl,and then tempered at 200℃.Its hardness,ultimate tensile strength,and wear rate reach551.4 HBW,1,438.2 MPa,and 0.48×10^(-2)mg·m^(-1),respectively.

关键词： low alloy wear-resistant steel quenching temperature cooling condition precipitate retained austenite wear resistance