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DNA Vaccine of SARS-Cov S Gene Induces Antibody Response in Mice

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 37-41页

作者： Ping zhao, Jin-Shan KE, zhao-Lin QIN, Hao REN, lan-juan zhao, Jian-Guo YU, Jun GAO, Shi-Ying ZHU, and Zhong-Tian QI (Department of Microbiology, Second Military Medical University, Shanghai 200433, China Department of Infectious Diseases, PLA Hospital No.88, Tai'an 271000, China ) Department of Microbiology Second Military Medical University Shanghai 200433 China.

The spike (S) protein, a main surface antigen of SARS-coronavirus (SARS-CoV), is one of themost important antigen candidates for vaccine design. In the present study, three fragments of the truncated S protein were expressed in E. coli, and analyzed with pooled sera of convalescence phase of SARS patients. The full length S gene DNA vaccine was constructed and used to immunize BALB/c mice. The mouse serum IgG antibody against SARS-CoV was measured by ELISA with E. coli expressed truncated S protein or SARS-CoV lysate as diagnostic antigen. The results showed that all the three fragments of S protein expressed by E. coli was able to react with sera of SARS patients and the S gene DNA candidate vaccine could induce the production of specific IgG antibody against SARS-CoV efficiently in mice with seroconversion ratio of 75% after 3 times of immunization. These findings lay some foundations for further understanding the immunology of SARS-CoV and developing SARS vaccines.

关键词： severe acute respiratory syndrome-coronavirus (SARS-CoV) spike protein DNA vaccine antibody

Phage display selection on whole cells yields a small peptide specific for HCV receptor human CD81

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Cell Research 2003年第6期13卷 473-479页

作者： JIE CAO, PING zhao, XIAO HUI MIAO2, lan juan zhao, LI JUN XUE, ZHONG TIAN QIDepartment of Microbiology, Second Military Medical University, Shanghai 200433, ChinaE-mail: qizt@*** Department of Infectious Diseases, Changzheng Hospital, Second Military Medical University, Shanghai200003, China Department of Microbiology Second Military Medical University Shanghai 200433 China.

The human CD81 (hCD81), the most recently proposed receptor of hepatitis C virus (HCV), can especifically bind to HCV envelope glycoprotein 2 (E2). In this study, hCD81-expressing murine NIH/3T3 cells were used to select hCD81-binding peptides from a phage displayed nonapeptide library (PVIII9aaCys). Eighteen of the 75 clones selected from the library showed specific binding to the hCD/81-expressing NIH/3T3 cells by enzyme linked immunosorbent assay (ELISA) and competitive inhibition test. Twelve out of the 18 clones shared the amino acid motif SPQYWTGPA. Sequence comparison of the motif showed no amino acid homology with the native HCV E2. The motif-containing phages could competitively inhibit the ability of HCV E2 binding to native hCD81-expressing MOLT-4 cells, and induce HCV E2 specific immune response in vivo. These results suggest that the selected motif SPQYWTGPA should be a mimotope of HCV E2 to bind to hCD81 molecules. Our findings cast new light on developing HCV receptor antagonists.

关键词： viral receptor hepatitis C virus cell-based selection hCD81-binding peptide phage display.

Function of nonstructural 5A protein of genotype 2a in replication and infection of HCV with gene substitution

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World Journal of Gastroenterology 2011年第29期17卷 3398-3406页

作者： Yong-Zhi Wang Wen-Bo Wang Ming-Mei Cao Wen Wang lan-juan zhao Gang Xu Hao Ren Zhong-Tian Qi Department of Cardiology No.254 Hospital PLATianjin 300142China Department of Microbiology Shanghai Key Laboratory of Medical BiodefenseSecond Military Medical UniversityShanghai 200433China

AIM:To explore the function of Nonstructural 5A(NS5A) protein of genotype 2a(JFH1)in the replication and infection of hepatitis C virus(HCV).METHODS:Intergenotypic chimera FL-J6JFH/J4NS5A was constructed by inserting NS5A gene from 1b stain HC-J4 by the overlapping polymerase chain reaction (PCR)method and the restriction enzyme *** vitro RNA transcripts of chimera,prototype J6JFH and negative control J6JFH1(GND)were prepared and transfected into Huh-7.5 cells with *** assay(IFA),fluorescence quantitative PCR and infection assay were performed to determine the protein expression and gene replication in Huh-7.5 ***:The HCV RNA levels in FL-J6JFH/J4NS5A chimera RNA transfected cells were significantly lower than the wild type at any indicated time point(2.58 ±5.97×106 vs 4.27±1.72×104,P=0.032).The maximal level of HCV RNA in chimera was 5.6±1.8× 104 GE/μg RNA at day 34 after transfection,while the wild type reached a peak level at day 13 which was 126 folds higher(70.65±14.11×105 vs 0.56±0.90 ×105,P=0.028).HCV proteins could also be detected by IFA in chimera-transfected cells with an obviously low *** assay showed that FL-J6JFH/J4NS5A chimera could produce infectious virus particles,ranging from 10±5 ffu/mL to 78.3±23.6 ffu/mL,while that of FL-J6JFH1 ranged from 5.8±1.5×102 ffu/mL to 2.5±1.4×104 ffu/***:JFH1 NS5A might play an important role in the robust replication of *** establishment of FL-J6JFH/J4NS5A provided a useful platform for studying the function of other proteins of HCV.

关键词： Hepatitis C virus Nonstructural 5A Chimera Cell culture-produced virus Replication Infection

Diagnostic Value of 18F-FDG PET/CT in Patients with Fever of Unknown Origin

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Diagnostic Value of 18F-FDG PET/CT in Patients with Fever of...

Silencing of SARS-CoV spike gene by small interfering RNA in HEK 293T cells

第二届传染病诊治高峰论坛暨2009年浙江省感染病、肝病学术年会

作者： Ji-Fang Sheng Zi-Ke Sheng Guo-Ping Sheng Hai-Ying Yu Hai-Jun Huang Jun Liu Dai-Rong Xiang lan-juan Li Kui zhao

Purpose Fever of unknown origin(FUO) is still a challenging problem in clinical *** present work is to evaluate fluorine-18 fluorodeoxyglucose(18F-FDG) positron emission tomography/computed tomography (PET/CT) in the ... 详细信息

关键词： FDG PET Diagnostic Value of 18F-FDG PET/CT in Patients with Fever of Unknown Origin

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Silencing of SARS-CoV spike gene by small interfering RNA in...

Comprehensive analysis of the association of EGFR,CALM3 and SMARCD1 gene polymorphisms with BMD in white women

第四届全国RNA进展研讨会

作者： zhao-ling Qin, Ping zhao, Xiao-lian Zhang, Jian-guo Yu, Ming-mei Cao, lan-juan zhao, Jie Luan, and Zhong-tian Qi. Department of Microbiology, Second Military Medical University, 800 Xiang-Yin Road, Shanghai, 200433

Two candidate small interfering RNAs (siRNAs) corresponding to severe acute respiratory syndrome-associated coronavirus (SARS-CoV) spike gene were designed and in vitro transcribed to explore the possibility of silencing SARS-CoV S gene. The plasmid pEGFP-optS, which contains the codon-optimized SARS-CoV S gene and expresses spike-EGFP fusion protein (S-EGFP) as silencing target and expressing reporter, was transfected with siRNAs into HEK 293T cells. At various time points of posttransfection, the levels of S-EGFP expression and amounts of spike mRNA transcript were detected by fluorescence microscopy, flow cytometry, western blot and real-time quantitative PCR, respectively. The results showed that the cells transfected with pEGFP-optS expressed S-EGFP fusion protein at a higher level

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Comprehensive analysis of the association of EGFR,CALM3 and ...

Salsolinol as an RNA m~6A methylation inducer mediates dopaminergic neuronal death by regulating YAP1 and autophagy

第十二届全国青年药学工作者最新科研成果交流会

作者： Qiu-Hong Zhou lan-juan zhao Ping Wang Rhamee Badr Xiao-Jing Xu Feng-Xiao Bu Joan Lappe Robert Recker Yu Zhou An Ye Bo-Ting Zhou Department of Endocrinology Xiangya HospitalCentral South University Osteoporosis Research Center Creighton University Medical Center Creighton University 601 N3Oth STSuite 4820OmahaNE 68131USA Department of Biostatistics & Bioinformatics School of Public Health and Tropical MedicineTulane UniversityNew OrleansLA 70112USA Department of Pharmacy Xiangya HospitalCentral South University Tulane University School of Medicine New OrleansLA 70112USA

Three genes EGFR（epidermal growth factor receptor）,CALM3（calmodulin 3,calcium-modulated protein 3,） and SMARCD1（SWI/SNF related,matrix associated,actin dependent regulator of charomatin,subfamily d,member 1） in white women played different roles in bone and/or fat *** the population-based investigation of 870 unrelated postmenopausal white women,CALM3 polymorphisms were significantly associated with femoral neck bone mineral density（FNK BMD）,hip BMD and spine *** and tobacco status also affected these BMD levels and hence were corrected for in our statistical ***:EGFR,CALM3 and SMARCD1 play their part in bone and/or fat metabolism,however,the correlation between polymorphisms of these three genes and body composition levels including BMD is yet *** and Methods:A population-based investigation of 870 white women was conducted.44 SNPs（Single Nucleotide Polymorphisms） in EGFR,CALM3 and SMARCD1 were chosen by the software of Haploview version 4.2,including those with potential functional *** candidate SNPs were genotyped by KASPar assay for association analysis with body composition levels at different *** correlation analysis was performed by the Pearson’s product-moment and Spearman rank-order test,and the family-wise error was corrected using the Wald test implement in ***:rsl2461917 in the 3’-flanking region of CALM3 gene was significantly associated wim FNK BMD（P=0.001）,hip BMD（PO.001） and spine BMD（P=0.001）;rsll083838 in the 5’-flanking region of CALM3 gene was associated with spine BMD（P=0.009）.After adjusting for multiple comparisons,rsl2461917 remained significant（Padjusted=0.033 for FNK BMD,Padjusted=0.006 for hip BMD and Padjusted=0.018 for spine BMD）.Conclusions:Our data shows that polymorphisms of the CALM3 gene in white women could contribute to variation in BMD at the hip,spine,and femoral neck.

关键词： osteoporosis bone mineral density genetics single nucleotide polymorphisms EGFR CALM3 SMARCDl

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Neural Regeneration Research 2025年第3期20卷 887-899页

作者： Jianan Wang Yuanyuan Ran Zihan Li Tianyuan zhao Fangfang Zhang juan Wang Zongjian Liu Xuechai Chen Beijing International Science and Technology Cooperation Base for Antiviral Drugs College of Chemistry and LifeBeijing University of TechnologyBeijingChina Department of Rehabilitation Beijing Rehabilitation HospitalCapital Medical UniversityBeijingChina

Salsolinol(1-methyl-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline,Sal)is a catechol isoquinoline that causes neurotoxicity and shares structural similarity with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,an environmental toxin that causes Parkinson's ***,the mechanism by which Sal mediates dopaminergic neuronal death remains *** this study,we found that Sal significantly enhanced the global level of N~6-methyladenosine(m~6A)RNA methylation in PC12 cells,mainly by inducing the downregulation of the expression of m~6A demethylases fat mass and obesity-associated protein(FTO)and alk B homolog 5(ALKBH5).RNA sequencing analysis showed that Sal downregulated the Hippo signaling *** m~6A reader YTH domain-containing family protein 2(YTHDF2)promoted the degradation of m~6A-containing Yes-associated protein 1(YAP1)mRNA,which is a downstream key effector in the Hippo signaling ***,downregulation of YAP1 promoted autophagy,indicating that the mutual regulation between YAP1 and autophagy can lead to *** findings reveal the role of Sal on m~6A RNA methylation and suggest that Sal may act as an RNA methylation inducer mediating dopaminergic neuronal death through YAP1 and *** results provide greater insights into the neurotoxic effects of catechol isoquinolines compared with other studies and may be a reference for assessing the involvement of RNA methylation in the pathogenesis of Parkinson's disease.

关键词： ALKBH5 autophagy FTO Hippo pathway m~6A Parkinson's disease RNA methylation salsolinol YAP1 YTHDF2

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Return to work in young and middle-aged colorectal cancer survivors:Factors influencing self-efficacy,fear,resilience,and financial toxicity

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World Journal of Gastroenterology 2025年第1期31卷 79-92页

作者： Dan Hu Yue Li Hua Zhang Lian-Lian Wang Wen-Wen Liu Xin Yang Ming-zhao Xiao Hao-Ling Zhang juan Li Department of Nursing The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Nursing National Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChildren’s Hospital of Chongqing Medical UniversityChongqing 400014China Department of Gastrointestinal Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Biomedical Science Advanced Medical and Dental InstituteUniversity Sains MalaysiaPenang 13200Malaysia Academic Affairs Office The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

BACKGROUND Return to work(RTW)serves as an indication for young and middle-aged colorectal cancer(CRC)survivors to resume their normal social ***,these survivors encounter significant challenges during their RTW ***,scientific research is necessary to explore the barriers and facilitating factors of returning to work for young and middle-aged CRC *** To examine the current RTW status among young and middle-aged CRC survivors and to analyze the impact of RTW self-efficacy(RTW-SE),fear of progression(FoP),eHealth literacy(eHL),family resilience(FR),and financial toxicity(FT)on their RTW *** A cross-sectional investigation was adopted in this *** September 2022 to February 2023,a total of 209 participants were recruited through a convenience sampling method from the gastrointestinal surgery department of a class A tertiary hospital in *** investigation utilized a general information questionnaire alongside scales assessing RTW-SE,FoP,eHL,FR,and *** analyze the factors that influence RTW outcomes among young and middle-aged CRC survivors,Cox regression modeling and Kaplan-Meier survival analysis were *** A total of 43.54%of the participants successfully returned to work,with an average RTW time of 100 *** regression univariate analysis revealed that RTW-SE,FoP,eHL,FR,and FT were significantly different between the non-RTW and RTW groups(P<0.05).Furthermore,Cox regression multivariate analysis identified per capita family monthly income,job type,RTW-SE,and FR as independent influencing factors for RTW(P<0.05).CONCLUSION The RTW rate requires further *** levels of RTW-SE and FR were found to significantly increase RTW among young and middle-aged CRC *** professionals should focus on modifiable factors,such as RTW-SE and FR,to design targeted RTW support programs,thereby facilitating their timely reintegration into mainstream society.

关键词： Return to work Colorectal neoplasms Return-to-work self-efficacy Fear of progression Family resilience Financial toxicity

Mediating role of health literacy in the relationship between social isolation and psychological distress among pre-frail older adults

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World Journal of Psychiatry 2025年第1期15卷 137-144页

作者： juan Fang Li-Hui Li Ming-Qian He Yue Ji Dong-Yan Lu Li-Bing Zhang Jin-lan Yao School of Medicine Huzhou UniversityHuzhou 313000Zhejiang ProvinceChina

BACKGROUND Frailty has become a significant public health *** recent increase in the number of frail older adults has led to increased attention being paid to psycho-logical care services in *** social isolation of pre-frail older adults can impact their psychological *** To explore the mediating effect of health literacy between social isolation and psychological distress among communitydwelling older adults with *** This descriptive cross-sectional study was conducted with 254 pre-frail older individuals aged 60 years and *** isolation,health literacy,and psycholo-gical distress were comprehensively measured using the Lubben Social Network Scale-6,12-item Short-Form Health Literacy Questionnaire,and the Kessler Psy-chological Distress *** were evaluated using the SPSS 27.0 package program and the PROCESS macro *** statistical analyses,correlation analyses,and bootstrap mediation tests were used to assess associations between the *** The results showed that social isolation had an effect on health literacy among pre-frail older adults(β=0.240,P<0.001),social isolation impact on psycho-logical distress pre-frail older adults(β=-0.415,P<0.001);health literacy was identified effect on psychological distress among pre-frail older persons(β=-0.307,P<0.001).Health literacy partially mediated the relationship between social isolation and psychological distress among community-dwelling older adults with pre-frailty,with a mediation effect of-0.074,accounting for 17.83%of the total effect.

关键词： Frail Ageing Psychological distress Health literacy Social isolation