检索结果-南通市图书馆

The Crop Journal 2024年第2期12卷 364-374页

作者： Caihua Qin Xing Fan Qianqian Fang lan ni Mingyi Jiang College of Life Sciences State Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationKey Laboratory of Crop Physiology Ecology and Production ManagementMinistry of AgricultureNanjing Agricultural UniversityNanjing 210095JiangsuChina

SubclassⅢsucrose nonfermenting1-related protein kinase 2s(SnRK2s)function in ABA and abiotic stress responses by unknown mechanisms.We found that osmotic stress/ABA-activated protein kinase 10(SAPK10),a member of rice SnRK2s,physically interacted with CBL-interacting protein kinase 1(OsCIPK1).OsCIPK1 expression was up-regulated by ABA and PEG treatment,and overexpression increased the ABA sensitivity of seed germination and root growth and plant osmotic stress tolerance.Osmotic stress or ABA-induced activation of OsCIPK1 is dependent on SAPK10.SAPK10 phosphorylates Thr-24 of OsCIPK1 in vitro,and this phosphorylation increases the activity of OsCIPK1 and positively regulates the function of OsCIPK1 in ABA responses and plant osmotic stress tolerance.This study suggests that OsCIPK1 is a direct phosphorylated substrate of SAPK10,and SAPK10-mediated phosphorylation of OsCIPK1 functions in ABA signaling and increases rice osmotic stress tolerance.

关键词： Rice ABA Osmotic stress OsCIPK1 SAPK10

Novel MIP gene mutation causes autosomal-dominant congenital cataract

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

International Journal of Ophthalmology(English edition) 2024年第3期17卷 454-465页

作者： Jing-lan ni Hua-Ming Wen Xiao-Sheng Huang Qian-Wen Li Jia-Min Cai Bao-Jian Fan Jun Zhao The Second Clinical Medical College Jinan UniversityShenzhen 518020Guangdong ProvinceChina Department of Ophthalmology Dongguan Chang’an HospitalDongguan 523843Guangdong ProvinceChina Shenzhen Eye Hospital Affiliated to Jinan University Shenzhen Eye InstituteShenzhen 518040Guangdong ProvinceChina Department of Oral&Maxillofacial Surgery Shenzhen Stomatology Hospital Affiliated to Shenzhen UniversityShenzhen 518040Guangdong ProvinceChina Department of Neurosurgery Massachusetts General Hospital and Harvard Medical SchoolBoston 02114MassachusettsUSA Department of Ophthalmology Shenzhen People’s Hospital(the Second Clinical Medical CollegeJinan Universitythe First Affiliated HospitalSouthern University of Science and Technology)Shenzhen 518020Guangdong ProvinceChina

●AIM:To identify disease-causative mutations in families with congenital cataract.●METHODS:Two Chinese families with autosomaldominant congenital cataract(ADCC)were recruited and underwent comprehensive eye examinations.Gene panel next-generation sequencing of common pathogenic genes of congenital cataract was performed in the proband of each family.Sanger sequencing was used to valid the candidate gene mutations and sequence the other family members for co-segregation analysis.The effect of sequence changes on protein structure and function was predicted through bioinformatics analysis.Major intrinsic protein(MIP)-wildtype and MIP-G29R plasmids were constructed and microinjected into zebrafish single-cell stage embryos.Zebrafish embryonic lens phenotypes were screened using confocal microscopy.●RESULTS:A novel heterozygous mutation(c.85G>A;p.G29R)in the MIP gene was identified in the proband of one family.A known heterozygous mutation(c.97C>T;p.R33C;rs864309693)in MIP was found in the proband of another family.In-silico prediction indicated that the novel mutation might affect the MIP protein function.Zebrafish embryonic lens was uniformly transparent in both wild-type PCS2+MIP and mutant PCS2+MIP.●CONCLUSION:Two missense mutations in the MIP gene in Chinese cataract families are identified,and one of which is novel.These findings expand the genetic spectrum of MIP mutations associated with cataracts.The functional studies suggest that the novel MIP mutation might not be a gain-of-function but a loss-of-function mutation.

关键词： congenital cataract major intrinsic protein missense mutation zebrafish model

The NADPH oxidase OsRbohA increases salt tolerance by modulating K^(+)homeostasis in rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The Crop Journal 2022年第6期10卷 1611-1622页

作者： Qingwen Wang lan ni Zhenzhen Cui Jingjing Jiang Chao Chen Mingyi Jiang National Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Crop Physiology Ecology and Production Management Ministry of Agriculture/College of Life SciencesNanjing Agricultural UniversityNanjing 210095JiangsuChina Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China Hunan Agricultural UniversityChangsha 410128HunanChina

Rice(Oryza sativa L.)is a staple cereal for more than two thirds of the world's population.Soil salinity severely limits rice growth,development,and grain yield.It is desirable to elucidate the mechanism of rice's salt-stress response.As the major source of H_(2)O_(2),NADPH oxidase(Rboh)is believed to be involved in salt-stress tolerance.However,the function and mechanism of rice Rboh in salt stress response remain unclear.In this study,we found that the expression of OsRbohA was up-regulated by NaCl treatment in the shoots and roots of rice seedlings.Knockout of OsRbohA reduced the tolerance of rice to salt stress.Knockout of OsRbohA blocked NaCl-induced increases of NADPH activity and H_(2)O_(2) content in roots.OsRboh A knockout inhibited root growth and disrupted K^(+)homeostasis by reducing the expression of K^(+) transporters and channel-associated genes(OsGORK,OsAKT1,OsHAK1,and OsHAK5)in roots under NaCl treatment.Under NaCl treatment,OsRbohA knockout also reduced subcellular K^(+) contents of the plasma membrane and soluble fraction.Overexpression of OsRbohA increased the expression of K^(+) transporters and channel-associated genes and reduced the loss of K^(+) ions in roots.These results indicate that OsRboh A-mediated H_(2)O_(2) accumulation modulates K^(+) homeostasis,thereby increasing salt tolerance in rice.

关键词： Rice OsRbohA H_(2)O_(2) K^(+)homeostasis Salt tolerance

A Review of Artificial Waterfalls in Gardens

在线全文

学校读者我要写书评

暂无评论

Journal of World Architecture 2024年第1期8卷 31-34页

作者： ni lan Tiantian Chen Qiushuang Fan Shiyu Mi Chongqing Institute of Engineering Chongqing 400056China

When artificial waterfalls are built in gardens,leaves from trees or vegetation often fall into the water storage pool and block the pipelines,requiring regular cleaning of pipelines.This not only increases the manpower needed for maintenance but also disrupts the use of the waterfalls.Therefore,it is necessary to design a new type of artificial waterfall that is suitable for gardens.The waterfall should incorporate features that make cleaning easier,including filter plates and water funnels to prevent clogging caused by fallen leaves and debris.

关键词： Intelligence Efficiency improvement Transportation pipeline Technical artificial waterfall

OsDMI3-mediated OsUXS3 phosphorylation improves oxidative stress tolerance by modulating OsCATB protein abundance in rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2022年第5期64卷 1087-1101页

作者： lan ni Qingwen Wang Chao Chen Shuang Wang Tao Shen Jingjing Jiang Zhenzhen Cui Kaiyue Li Qiqing Yang Mingyi Jiang College of Life Sciences National Key Laboratory of Crop Genetics and Germplasm EnhancementKey Laboratory of Crop Physiology Ecology and Production ManagementMinistry of AgricultureNanjing Agricultural UniversityNanjing 210095China Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China Hunan Agricultural UniversityChangsha 410128China

Calcium(Ca^(2+))/calmodulin(CaM)-dependent protein kinase(CCaMK)is an important positive regulator of antioxidant defenses and tolerance against oxidative stress.However,the underlying molecular mechanisms are largely unknown.Here,we report that the rice(Oryza sativa)CCa MK(OsDMI3)physically interacts with and phosphorylates OsUXS3,a cytosol-localized UDP-xylose synthase.Genetic and biochemical evidence demonstrated that OsUXS3 acts downstream of OsDMI3 to enhance the oxidative stress tolerance conferred by higher catalase(CAT)activity.Indeed,OsUXS3 interacted with CAT isozyme B(OsCATB),and this interaction was required to increase OsCATB protein abundance under oxidative stress conditions.Furthermore,we showed that OsDMI3 phosphorylates OsUXS3 on residue Ser-245,thereby further promoting the interaction between OsUXS3 and OsCATB.Our results indicate that OsDMI3 promotes the association of OsUXS3 with OsCATB to enhance CAT activity under oxidative stress.These findings reveal OsUXS3 as a direct target of OsDMI3 and demonstrate its involvement in antioxidant defense.

关键词： Ca2+/calmodulin-dependent protein kinase catalase oxidative stress rice UDP-xylose synthase

A Brief Discussion on the landscape Balconies of New Buildings

在线全文

学校读者我要写书评

暂无评论

Journal of Architectural Research and Development 2024年第1期8卷 38-42页

作者： ni lan Feng Huang Xutong Liu Xin Xiang Junjie Chen Chongqing Institute of Engineering Chongqing 400056China

With the acceleration of urbanization and the improvement of people’s living standards,people have increasingly higher requirements for the living environment,especially in the design of outdoor balconies.However,existing landscape balconies generally suffer from insufficient shading and rainwater drainage problems,which affects people’s living experience.In response to this problem,this article proposes a new design scheme for building landscape balconies to solve the existing problems of landscape balconies.This design plan fully considers the user’s usage needs and scenarios,improves the use value and comfort of the balcony through technical means,and achieves intelligence and functionality.

关键词： landscape balcony Intelligence Functionalization Market analysis

Phosphorylation of OsRbohB by the protein kinase OsDMI3 promotes H_(2)O_(2) production to potentiate ABA responses in rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular Plant 2023年第5期16卷 882-902页

作者： Qingwen Wang Tao Shen lan ni Chao Chen Jingjing Jiang Zhenzhen Cui Shuang Wang Fengjuan Xu Runjiao Yan Mingyi Jiang College of Life Sciences State Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationKey Laboratory of Crop Physiology Ecology and Production ManagementMinistry of AgricultureNanjing Agricultural UniversityNanjing 210095China

In rice, the Ca^(2+)/calmodulin-dependent protein kinase OsDMI3 is an important positive regulator of abscisic acid (ABA) signaling. In ABA signaling, H_(2)O_(2) is required for ABA-induced activation of OsDMI3, which in turn increase H_(2)O_(2) production. However, how OsDMI3 regulates H_(2)O_(2) production in ABA signaling remains unknown. Here we show that OsRbohB is the main NADPH oxidase involved in ABA-induced H_(2)O_(2) production and ABA-mediated physiological responses. OsDMI3 directly interacts with and phosphorylates OsRbohB at Ser-191, which is OsDMI3-mediated site-specific phosphorylation in ABA signaling. Further analyses revealed that OsDMI3-mediated OsRbohB Ser-191 phosphorylation positively regulates the activity of NADPH oxidase and the production of H_(2)O_(2) in ABA signaling, thereby enhancing the sensitivity of seed germination and root growth to ABA and plant tolerance to water stress and oxidative stress. Moreover, we discovered that the OsDMI3-mediated OsRbohB phosphorylation and H_(2)O_(2) production is dependent on the sucrose non-fermenting 1-related protein kinases SAPK8/9/10, which phosphorylate OsRbohB at Ser-140 in ABA signaling. Taken together, these results not only reveal an important regulatory mechanism that directly activates Rboh for ABA-induced H_(2)O_(2) production but also uncover the importance of this regulatory mechanism in ABA signaling.

关键词： abscisic acid Ca^(2+)/calmodulin-dependent protein kinase NADPH oxidases protein phosphorylation rice

Targeting redox-altered plasticity to reactivate synaptic function: A novel therapeutic strategy for cognitive disorder

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2021年第3期11卷 599-608页

作者： Pei Wang Fang Wang lan ni Pengfei Wu Jianguo Chen Department of Pharmacology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Key Laboratory of Neurological Diseases(HUST) Ministry of Education of ChinaWuhan 430030China The Key Laboratory for Drug Target Researches and Pharmacodynamic Evaluation of Hubei Province Wuhan 430030China Laboratory of Neuropsychiatric Diseases the Institute of Brain ResearchHuazhong University of Science and TechnologyWuhan 430030China

Redox-altered plasticity refers to redox-dependent reversible changes in synaptic plasticity via altering functions of key proteins, such as N-methyl-D-aspartate receptor(NMDAR). Age-related cognitive disorders includes Alzheimer’s disease(AD), vascular dementia(VD), and age-associated memory impairment(AAMI). Based on the critical role of NMDAR-dependent long-term potentiation(LTP) in memory, the increase of reactive oxygen species in cognitive disorders, and the sensitivity of NMDAR to the redox status, converging lines have suggested the redox-altered NMDAR-dependent plasticity might underlie the synaptic dysfunctions associated with cognitive disorders. In this review, we summarize the involvement of redox-altered plasticity in cognitive disorders by presenting the available evidence. According to reports from our laboratory and other groups, this "redox-altered plasticity" is Hydrogen sulfidemore similar to functional changes rather than organic injuries, and strategies targeting redox-altered plasticity using pharmacological agents might reverse synaptic dysfunctions and memory abnormalities in the early stage of cognitive disorders. Targeting redox modifications for NMDARs may serve as a novel therapeutic strategy for memory deficits.

关键词： Reactive oxygen species N-Methyl-D-aspartate receptor Oxidative stress Synaptic plasticity Long-term potentiation Cognitive disorder Learning and memory Hydrogen sulfide

S-methyl-L-cysteine Protects against Antimycin A-induced Mitochondrial Dysfunction in Neural Cells via Mimicking Endogenous Methionine-centered Redox Cycle

在线全文

学校读者我要写书评

暂无评论

Current Medical Science 2020年第3期40卷 422-433页

作者： lan ni Xin-lei GUAN Fu-feng CHEN Peng-fei WU Department of Pharmacology School of Basic MedicineTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pharmacy Wuhan Puai HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430033China Tongji-Rongcheng Center for Biomedicine Wuhan 430030China Key Laboratory for Drug Target Researches and Pharmacodynamic Evaluation of Hubei Province Wuhan 430030China

Mitochondrial superoxide overproduction is believed to be responsible for the neurotoxicity associated with neurodegeneration.Mitochondria-targeted antioxidants,such as MitoQ,have emerged as potentially effective antioxidant therapies.Methionine sulfoxide reductase A(MsrA)is a key mitochondrial-localized endogenous antioxidative enzyme and it can scavenge oxidizing species by catalyzing the methionine(Met)-centered redox cycle(MCRC).In this study,we observed that the natural L-Met acted as a good scavenger for antimycin A-induced mitochondrial superoxide overproduction in PC12 cells.This antioxidation was largely dependent on the Met oxidase activity of MsrA.S-methyl-L-cysteine(SMLC),a natural analogue of Met that is abundantly found in garlic and cabbage,could activate the Met oxidase activity of MsrA to scavenge free radicals.Furthermore,SMLC protected against antimycin A-induced mitochondrial membrane depolarization and alleviated 1-methyl-4-phenylpyridinium(MPP+)-induced neurotoxicity.Thus,our data highlighted the possibility for SMLC supplement in the detoxication of mitochondrial damage by activating the Met oxidase activity of MsrA.

关键词： methionine sulfoxide reductase A Met oxidase S-methyl-L-cysteine neurotoxin 1-methyl-4-phenylpyridinium

The C_2H_2 -type Zinc Finger Protein ZFP182 is Involved in Abscisic Acid-Induced Antioxidant Defense in Rice

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2012年第7期54卷 500-510页

作者： Hong Zhang lan ni Yanpei Liu Yunfei Wang Aying Zhang Mingpu Tan Mingyi Jiang College of Life Sciences Nanjing Agricultural UniversityNanjing 210095China National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing 210095China

C2H2-type zinc finger proteins （ZFPs） are thought to play important roles in modulating the responses of plants to drought, salinity and oxidative stress. However, direct evidence is lacking for the involvement of these ZFPs in abscisic acid （ABA）-induced antioxidant defense in plants. In this study, the role of the rice （Oryza sativa L. sub.japonica cv. nipponbare） C2H2-type ZFP ZFP182 in ABA-induced antioxidant defense and the relationship between ZFP182 and two rice MAPKs, OsMPK1 and OsMPK5 in ABA signaling were investigated. ABA treatment induced the increases in the expression of ZFP182, OsMPK1 and OsMPK5, and the activities of superoxide dismutase （SOD） and ascorbate peroxidase （APX） in rice leaves. The transient gene expression analysis and the transient RNA interference （RNAi） analysis in protoplasts showed that ZFP182, OsMPK1 and OsMPK5 are involved in ABA-induced up-regulation in the activities of SOD and APX. Besides, OsMPK1 and OsMPK5 were shown to be required for the up-regulation in the expression of ZFP182 in ABA signaling, but ZFP182 did not mediate the ABA-induced up-regulation in the expression of OsMPK1 and OsMPKS. These results indicate that ZFP182 is required for ABA-induced antioxidant defense and the expression of ZFP182 is regulated by rice MAPKs in ABA signaling.

关键词： Zinc finger protein abscisic acid antioxidant defense rice MAPK.