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Isolation and characterization of marine-derived actinomycetes with cytotoxic activity from the Red Sea coast

Asian Pacific Journal of Tropical Biomedicine 2016年第8期6卷 651-657页

作者： Mohamed Saleh Abdelfattah Mohammed Ismail Youssef elmallah Usama Wahid Hawas lamia taha abou el-kassema Mennat Allah Gamal Eid Marine Natural Products Research Unit Faculty of Science Helwan University Marine Chemistry Department Faculty of Marine Sciences King Abdulaziz University Pharmacognosy Department National Research Centre

Objective: To isolate and evaluate the cytotoxic activity of different actinomycetes species isolated from the Red Sea coast in Sharm el-Sheikh, ***: Forty actinomycetes strains were isolated from different sediments and seawater samples collected from the Red Sea coast in Egypt. Actinomycetes were recognized by morphological and microscopic examinations. Cell viability and cytotoxicity induced by the crude extracts on breast cancer cell lines MDA-MB-231 were assessed using methylene blue assay. The strains with promising cytotoxic activity were identified by sequencing and amplifying the 16 S r RNA genes. The antibacterial activities of the crude extracts were performed using Kirby–Bauer disc diffusion ***: The results indicated that five ethyl acetate extracts exhibited cytotoxicity towards breast cancer cell lines MDA-MB-231. The highest cytotoxic activity was found for the ethyl acetate extracts of EGY2 and EGY39. The isolate EGY3 was identified as a new Streptomyces species, while the actinomycete EGY22 was found to be a member of the genus Nocardiopsis sp. The crude extract of the isolate EGY8 showed slightly high antimicrobial activity against different test ***: The results of the present study reveal that marine sediments of the Red Sea are a potent source of novel species of actinomycetes. The isolates may be useful in discovery of novel bioactive compounds and an important step in the development of microbial natural product research.

关键词： Red Sea Breast cancer Actinomycetes 16S rRNA Cytotoxicity

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Clinical and Radiological Localization of Language Dysfunctions in Acute Ischemic Stroke

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Open Journal of Medical Imaging 2014年第2期4卷 80-85页

作者： Amira Ahmed Zaki Dwidar taha Kamel Alloush Mahmoud Youssef abou el-ella Nahed Salah el-Din Ahmed Karima Moustafa Maher Maha Ali Mohamed Nada Mohamed Hamdy Ibrahim Faculty of Medicine Ain Shams University Cairo Egypt

Objective: To study the effect of cerebral ischemic vascular insult on the localization of language areas as regard to automatic speech, initiation, and mutism. Materials and Methods: Seventy ischemic stroke patients were selected within the first 48 h of the onset of stroke with left hemispheric stroke with aphasia. Inclusion criteria: any age above seven years, both sexes, first ever clinical stroke, admitted within the first 2 days of stroke insult. Patients underwent careful history taking, neurological examination, routine laboratory investigations, echo cardiography, and carotid duplex and language assessments. Results: Automatic speech disorders were mainly seen along left insular, left supramarginal and left frontal inferior orbital lesions. Initiation difficulty is seen mainly along as left insular, left temporal superior and left periventricular lesions. Mutism is seen along left external capsule, left internal capsule, left pallidum, left frontal inferior orbital and left inferior trigone lesions. Conclusions: Deep periventricular white matter and insular area in the dominant hemisphere play an important role in many language tasks. Thus aphasia is not a mere cortical function.

关键词： Clinicoradiological Localization Aphasia Stroke White Matter Perventricular

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Effect of Three Different Growth Media on Yield and Oil Constituents of Rosemary （Rosmarinus officinalis L.） under Protected Agriculture Conditions

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Journal of Agricultural Science and Technology(A) 2014年第5期4卷 395-403页

作者： Kholod F. abou Seedo Ahmed A. Salih Ahmed A. taha Arid Zones Sciences Programme Graduate College Arabian Gulf University Manama Kingdom of Bahrain Department of Chemistry Faculty of Science University of Bahrain Manama Kingdom of Bahrain

Medicinal plants provide an important source of cure since ancient time. Poor soil resources, scarce and saline water and the harsh environment limited the production of plants in the Arabian Gulf countries. This study aimed to investigate the production potential of rosemary (Rosmarinus officinalis L.) grown on different growth media under greenhouse conditions. Three growth media agricultural soil, compost and hydroponic system were used, whereas tuff (inert volcanic material) was used as substrate. The result indicated that the high salinity of the agricultural soil limited growth and oil yield in rosemary. Shoot height increased in 11 weeks, by 62%, 65% and 114% in plants grown in agricultural soil, hydroponic system and compost, respectively. Na content in plants grown in agricultural soil was significantly higher than in plants grown in the other treatment. Essential oil yield in plants grown in compost exceeded those in agricultural soil by 114%. Essential oil content (0.66%-1.5% w/w) and chemical constituents concentrations did not change significantly with growth media. The main constituents, more or less, are comparable to essential oils constituents reported from other countries. In comparison, better yields are obtained for individual components of the oils of plants grown under our green house conditions. This study demonstrated the great potential of commercial production of rosemary in the greenhouse without compromising the oil quality and oil yield.

关键词：生长介质农业土壤石油产量化学成分迷迭香药用植物保护温室条件

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