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Molecular basis of the anti-inflammatory potential of a diarylheptanoid in murine macrophage RAW 264.7 cells

Advances in Biological Chemistry 2013年第6期3卷 541-548页

作者： Bharathi Raja Rajaganapathy Karthikeyan Thirugnanam Muthusamy Velusamy Shanmuganathan Anand Singaravelu lakshmi baddireddi subadhra Tissue Culture and Drug Discovery Lab Centre for Biotechnology Anna University Chennai India Tissue Culture and Drug Discovery Lab Centre for Biotechnology Anna University Chennai India 2National Institute of Nutrition (Indian Council of Medical Research) Hyderabad India National Institute of Nutrition (Indian Council of Medical Research) Hyderabad India

Natural products play a significant role in human health in relation to the prevention and treatment of inflammatory disorders. In this study, we examined the molecular basis of the anti-inflammatory potential of a diarylheptonoid (DAH) isolated from Alpinia officinarum hexane extract (AOHE) with special emphasis on their ability to modulate the nuclear factor-κB (NF-кB) signaling involved in the inflammatory response. Measurement of Nitrite by Griess reaction which revealed the effect of DAH in RAW 264.7 macrophages showed an inhibition in the nitric oxide production through the suppression of inducible nitric oxide synthase (iNOS) gene level expression. NF-кB reporter gene assay suggests inhibition of NF-кB transcriptional activity, thus inhibiting LPS-induced phosphorylation and degradation of IкBα and a downregulation of NF-кB protein expression confirms the immunomodulatory effect of DAH. Furthermore, downregulation in the gene level expression of NF-кB signaling markers such as IL-1β, TNF-α and COX-2 suggests the anti-inflammatory potential of DAH via inhibition of NF-кB activation.

关键词： Diarylheptanoid NF-кB Lipopolysaccharide TNF-α COX-2 iNOS Cytokines

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Use of a naturally occurring codon bias for identifying topoisomerase mutations in ciprofloxacin resistant <i>Escherichia coli</i>using PCR and future prospects with other bacterial genera: A pilot study

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Advances in Biological Chemistry 2012年第4期2卷 366-371页

作者： Subramanian Krishnan Dheepikaa Balasubramanian B. Appala Raju baddireddi subadhra lakshmi Department of Microbiology PSG Institute of Medical Sciences and Research Coimbatore India Division of Infectious Diseases Department of Pediatrics The Saban Research Institute Children’s Hospital Los Angeles University of Southern California School of Medicine Los Angeles USA Tissue Culture and Drug Discovery Laboratory Centre for Biotechnology Anna University Chennai India

We developed a novel PCR method aimed at identi- fying and amplifying native codon sequences of muta- tion-prone amino acids in DNA gyrase implicated in quinolone resistance using a naturally occurring co- don bias in... 详细信息

关键词： Escherichia coli Gyrase A Codon Bias Mutation Ciprofloxacin Resistance PCR

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