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On-chip detection of a single nucleotide polymorphism without polymerase amplification

Nano Research 2014年第9期7卷 1302-1310页

作者： Jinhee Han Matthew Tan lakshmana sudheendra Robert H. Weiss Ian M. Kennedy Department of Mechanical and Aerospace Engineering University of California Davis California 95616 USA Division of Nephrology Department of Internal Medicine University of California Davis California 95616 USA Medical Service Sacramento VA Medical Center Sacramento California 95655 USA

A nanoparticle-assembled photonic crystal （PC） array was used to detect single nucleotide polymorphism （SNP）. The assay platform with PC nanostructure enhanced the fluorescent signal from nanoparticle-hybridized DNA complexes due to phase matching of excitation and emission. Nanoparticles coupled with probe DNA were trapped into nanowells in an array by using an electrophoretic particle entrapment system. The PC/DNA assay platform was able to identify a 1 base pair （bp） difference in synthesized nucleotide sequences that mimicked the mutation seen in a feline model of human autosomal dominant polycystic kidney disease （PKD） with a sensitivity of 0.9 fg/mL （50 aM）-sensitivity, which corresponds to 30 oligos/array. The reliability of the PC/DNA assay platform to detect SNP in a real sample was demonstrated by using genomic DNA （gDNA） extracted from the urine and blood of two PKD-wild type and three PKD positive cats. The standard curves for PKD positive （PKD＋） and negative （PKD-） DNA were created using two feline-urine samples. An additional three urine samples were analyzed in a similar fashion and showed satisfactory agreement with the standard curve, confirming the presence of the mutation in affected urine. The limit of detection （LOD） was 0.005 ng/mL which corresponds to 6 fg per array for gDNA in urine and blood. The PC system demonstrated the ability to detect a number of genome equivalents for the PKD SNP that was very similar to the results reported with real time polymerase chain reaction （PCR）. The favorable comparison with quantitative PCR suggests that the PC technology may find application well beyond the detection of the PKD SNP, into areas where a simple, cheap and portable nucleic acid analvsis is desirable.

关键词： photonic crystal array single nucleotidepolymorphisms DNA polycystic kidney disease real time polymerasechain reaction (PCR)

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Cooperation for Common Prosperity

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ChinAfrica 2023年第8期15卷 18-19页

作者： sudheendra KULKARNI the Forum for a New South Asia

Few saw BRICS emerging as one of the most successful and resilient multilateral platforms it has *** global order is currently undergoing tremendous *** old world order dominated by the West,the United States in particular,has been *** a new world order has yet to *** transitional period of turbulence,turmoil and uncertainty calls for multilateral forums like BRICS to raise voices throughout Asia,Africa and Latin America to join peace-loving people in the West,advocating for a world order based on peace,equitable development,and global security for all.

关键词： peace Latin turbulence

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New Year Prospects for China-India Relations

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中印对话（英文版） 2023年第1期 14-15页

作者： sudheendra Kulkarni Forum for a New South Asia

China and India need more than trade and *** most important are bilateral cooperation,people-to-people exchange,cultural exchange,and media exchange.

关键词： exchange exchange. India

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BRICS Cooperationn for Common Prosperity

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中印对话（英文版） 2023年第3期 14-15页

作者： sudheendra Kulkarni Forum for a New South

BRICs is in the interests of all people who love peace and equitable development,favor global cooperation,and advocate *** has emerged as one of the most successful and resilient multilateral platforms in the *** glob... 详细信息

关键词： peace Prosper emerged

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BRI:Bringing People and Countries Together

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中印对话（英文版） 2023年第5期37卷 6-8页

作者： sudheendra Kulkarni 不详

The Belt and Road Initiative(BRI)is the most important and ambitious connectivity and cooperation initiative in the entire history of *** initiative of this kind or scale had been attempted by any other country *** it progresses,the BRI will clearly change the destinies of many participating countriesaswellasthe destiny of all *** the dawn of history,whenever roads were built,theyfostered prosperity and development.

关键词： Together whenever BRI

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LEARNING FROM CPC'S SUCCESS

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China Report ASEAN 2021年第12期6卷 52-54页

作者： sudheendra Kulkarni the Forum for a New South Asia

The Communist Party of China(CPC)marked its 100th anniversary on July 1,*** the thousands of celebratory events taking place was a ballet by the National Ballet of China depicting two famous Chinese *** one,a 90-year-old“foolish man”named Yugong achieves the impossible mission of removing two huge mountains to create a pathway for villagers to get connected and also to increase land under *** the other myth.

关键词： removing CPC mountains

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China's Poverty Eradication Lessonss for India and Other Developing Countries

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中印对话（英文版） 2023年第4期36卷 40-42页

作者： sudheendra Kulkarni the Forumfora New South Asia

Anyone seeking to eradicate poverty in India should know all about China's ***'s approach to overcoming poverty is multi-dimensional and *** the beginning of the reform and opening up in the late 1970s,China has lifted nearly 800 million people out of extreme *** reached the poverty eradication target in the United Nations'2030 Agenda for Sustainable Development 10 years ahead of schedule.

关键词： Sustainable seeking India

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P* CHIRAL AMINOPHOSPHINE COMPLEXES OF COPPER （Ⅰ）:SYNTHESIS AND X-RAY STRUCTURAL CHARACTERIZATION

P* CHIRAL AMINOPHOSPHINE COMPLEXES OF COPPER （Ⅰ）:SYNTHESI...

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第17届国际磷化学大会

作者： T.Arun Luiz Babu Varghese M.N.sudheendra Rao Department of Chemistry Indian Institute of Technology MadrasChennai-600036 （India） Sophisticated Analytical Instruments Facility Indian Institute of Technology MadrasChennai-600036 （India） Department of Chemistry Indian Institute of Technology MadrasChennai-600036 （India）

＜正＞Chemistry of phosphine complexes with copper （Ⅰ） is an actively investigated area. Different structural types with varying coordination number at copper have been realized.

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应用支气管钳技术取出尖端嵌入的下腔静脉滤器:单中心的经验

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国际医学放射学杂志 2015年第4期 387-387页

作者： S.W.Stavropoulos B.H.Ge J.I.Mondschein R.D.Shlansky-Goldberg D.sudheendra S.O.Trerotola 孙超 Division of Interventional Radiology Department of Radiology Hospital of the University of Pennsylvania Perelman

摘要目的评估应用支气管钳取出尖端嵌入的下腔静脉(IVC)滤器的可行性。材料与方法本研究经机构伦理委员会批准并符合HIPAA。回顾性纳入2005年1月—2014年4

关键词：腔静脉滤器 IVC 支气管气管钳

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