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Molecular Cloning and Expression of Polyphenoloxidase Genes from the Mushroom, Agaricus bisporus

Agricultural Sciences in China 2011年第2期10卷 185-194页

作者： LI Nan-yi CAI Wei-ming JIN Qun-li qin Qiao-ping RAN Fu-lai College of Agriculture and Food Science Zhejiang Agricultural and Forestry UniversityHangzhou 311300P.R.China Institute of Horticulture Zhejiang Academy of Agricultural SciencesHangzhou 310021P.R.China Odessa College University of AveOdessa 79764USA

The polyphenoloxidase（PPO） is the key enzyme considered to be responsible for mushroom *** using homology cloning and rapid amplification of cDNA ends（RACE）,two new PPO genes and the corresponding cDNA were identified from the fruit bodies of Agaricus bisporus（AbPPO3 and AbPPO4,GenBank accession ***936494 and GU936493,respectively）.The genomic DNA sequences of AbPPO3 and AbPPO4 are 2 080 and 2 189 bp in length,respectively,encoding putative polypeptides of approximately 66 and 68 *** deduced amino acid sequences show characteristic features of two copper-binding domains conserved in the type III copper proteins including fungal polyphenol *** comparisons indicate that AbPPO3 and AbPPO4 present 55.3% similarity to each other（48% identity）.We also obtained more than 1.5-kb long sequences upstream of the start codon of the AbPPO3 and AbPPO4 and recognized them as their respective putative *** of the two PPO promoter regions show that they contain abundant cis-acting elements which are probably responsible for anaerobic induction,light,wound,stress,and auxin ***-quantitative RT-PCR results indicate that AbPPO3 and AbPPO4 were highly expressed in the mature fruit bodies and up-regulated after 2-d storage of *** results suggest that AbPPO3 and AbPPO4 may play roles in *** browning and pigmentation during development and postharvest storage and the elements in promoters may act as regulatory elements for the inducible expression of AbPPO3 and *** successful cloning and expression analysis of AbPPO3 and AbPPO4 warrant a further investigation on the structure and function of *** PPO which points to the possible targets for genetic manipulation.

关键词： polyphenoloxidase Agaricus bisporus tyrosinase promoter browning

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Eu^(3+)和Tb^(3+)掺杂硅酸钇纳米粉体的合成与表征

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发光学报 2012年第2期33卷 150-154页

作者：张烨吴琳君秦来顺中国计量学院材料科学与工程学院浙江杭州310018

采用溶胶-凝胶法合成了Y2SiO5∶Eu和Y2SiO5∶Tb纳米粉体,使用X射线衍射仪、扫描电镜等对粉体的结构、形貌和发光性能进行了分析,研究了Eu3+和Tb3+不同浓度掺杂硅酸钇纳米粉体的发光性能。在紫外光激发下,所获得的Y2SiO5∶Eu纳米粉体的... 详细信息

采用溶胶-凝胶法合成了Y2SiO5∶Eu和Y2SiO5∶Tb纳米粉体,使用X射线衍射仪、扫描电镜等对粉体的结构、形貌和发光性能进行了分析,研究了Eu3+和Tb3+不同浓度掺杂硅酸钇纳米粉体的发光性能。在紫外光激发下,所获得的Y2SiO5∶Eu纳米粉体的主发射峰均位于612 nm,对应于Eu3+的5D0→7F2跃迁;Y2SiO5∶Tb纳米粉体的主发射峰均位于540 nm,对应于Tb3+的5D4→7F5跃迁。

关键词：硅酸钇纳米粉体发光性能

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A review of ARGs in WWTPs:Sources,stressors and elimination

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Chinese Chemical Letters 2020年第10期31卷 2603-2613页

作者： Kena qin Liangliang Wei Jianju Li Bo lai Fengyi Zhu Hang Yu qingliang Zhao Kun Wang State Key Laboratory of Urban Water Resources and Environment(SKLUWRE) School of EnvironmentHarbin Institute of TechnologyHarbin 150090China State Key Laboratory of Hydraulics and Mountain River Engineering College of Architecture and EnvironmentSichuan UniversityChengdu 610065China

Antibiotic re sistance genes(ARGs)in aquatic enviro nments,which seriously endanger human health and ecological safety,have become a worldwide concern due to their easy diffusion and *** treatment plants(WWTPs),which receive resistant bacteria and ARGs from a wide variety of sources(i.e.,livestock farms,hospitals,antibiotic manufactures,and households),are regarded as important emission sources of aquatic *** review presents a quantitative profile of the majority sources of ARGs in the influent of WWTPs and discusses the potential factors that affect the concentration distribution of ***,a noteworthy existence of ARGs,which ranged from 1 E+05 to 1 E+11 copies/mL,was detected in livestock breeding wastewater,and household wastewater(caused by the unlimited utilization of antibiotics)was determined to be the predominant contributor of ARGs in *** summarized the selective pressure on ARGs and determined the positive correlation of the concentration of ARGs and the existence of many containments,including antibiotics,heavy metals(Zn and Cu were frequently reported),quaternary ammonium compounds,*** the last section,physical,chemical,and biological treatments for the removal of ARGs and their effluent in WWTPs are discussed and prospective future studies are summarized.

关键词： Antibiotics Antibiotics resistance genes Sources Stressors Artificial treatments

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How high can fracture porosity become in the ultra-deep subsurface?

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Geoscience Frontiers 2023年第5期14卷 233-240页

作者： Jin lai Fei Zhao Mei Zhang Tianyu Bai Yuyue Huang Dong Li Guiwen Wang Ziqiang qin State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum(Beijing)Beijing 102249China College of Geosciences China University of Petroleum(Beijing)102249China Department of Petroleum Engineering University of WyomingLaramieWY 82071USA

Knowledge of how high can fracture porosity become in the ultra-deep burial conditions is important but remains *** aperture and porosity are measured using X-ray computed tomography(CT)at atmospheric pressure and then calculated by image *** attention is paid to how high fracture porosity can become in ultra-deep(>6000 m)settings,and which situations will result in high fracture *** situ stress magnitudes,which can be calculated using well logs,control fracture performances,and dissolution along fracture improve fracture porosity at ultra-deep burial *** horizontal stress difference(Dr45 MPa),a high degree of dissolution along the fracture dramatically increases fracture *** will result in the vuggy fracture planes and improve fracture porosity up to 2.0%.The results provide insights into the detection,characterization,and modeling of subsurface fractures.

关键词： Fracture porosity Image log Ultra-deep In-situ stress Dissolution

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Efficacy of Biejiajian Pill on Intestinal Microbiota in Patients with Hepatitis B Cirrhosis/Liver Fibrosis:A Randomized Double-Blind Controlled Trial

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Chinese Journal of Integrative Medicine 2023年第9期29卷 771-781页

作者： CHI Xin CHENG Dan-ying SUN Xiu LIU shun-ai WANG Rong-bing CHEN qin XING Hui-chun Center of Liver Diseases Division 3 Beijing Ditan HospitalCapital Medical UniversityBeijing100015China Beijing Key Laboratory of Emerging Infectious Diseases Institute of Infectious DiseaseBeijing Ditan HospitalCapital Medical UniversityBeijing100015China National Center for Infectious Diseases Beijing100015China Peking University Ditan Teaching Hospital Beijing100015China Central of Integrated Chinese and Western Medicine Beijing Ditan HospitalCapital Medical UniversityBeijing100015China China Traditional Chinese Medicine Holdings Co.Limited Guangzhou528303China

Objective:To analyze the efficacy of Biejiajian Pill(BJJP)on intestinal microbiota in patients with hepatitis B cirrhosis/liver fibrosis,and explore its relationship with liver ***:This was a prospective,randomized double-blind controlled *** the stratified block randomization method,35 patients with hepatitis B liver cirrhosis/liver fibrosis were randomly assigned(1:1)to receive entecavir(0.5 mg/d)combined with BJJP(3 g/time,3 times a day)or placebo(simulator as control,SC group,simulator 3 g/time,3 times a day)for 48 *** and stool samples were collected from patients at baseline and week 48 of treatment,*** and renal functions as well as hematological indices were *** samples were analyzed by 16S rDNA V3-V4 high-throughput sequencing,and intestinal microbiota changes in both groups before and after treatment were compared,and their correlations with liver fibrosis were ***:Compared with the SC group,there was no significant difference in liver function,renal function and hematology indices in the BJJP group,however,the improvement rate of liver fibrosis was higher in the BJJP group(94.4%vs.64.7%,P=0.041).Principal coordinate analysis(PCoA)based on weighted Unifrac distance showed significant differences in intestinal microbiota community diversity before and after BJJP treatment(P<0.01 and P=0.003),*** 48 weeks'treatment,the abundance levels of beneficial bacteria(Bifidobacteria,Lactobacillus,Faecalibacterium and Blautia)increased,whereas the abundance levels of potential pathogenic bacteria,including Escherichia coli,Bacteroides,Ruminococcus,Parabacteroides and Prevotella decreased,among which Ruminococcus and Parabacteroides were significantly positively correlated with degree of liver fibrosis(r=0.34,P=0.04;r=0.38,P=0.02),*** microbiota in the SC group did not change significantly throughout the whole process of ***:BJJP had a certain regulatory effe

关键词： Biejiajian Pill chronic hepatitis B liver fibrosis liver cirrhosis intestinal microbiota randomized controlled trial Chinese medicine

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Association of MBOAT7 rs641738 polymorphism with hepatocellular carcinoma susceptibility:A systematic review and meta-analysis

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World Journal of Gastrointestinal Oncology 2023年第12期15卷 2225-2236页

作者： Min lai Ya-Lu qin Qiong-Yu Jin Wen-Jing Chen Jia Hu Department of Gastroenterology the Third Affiliated Hospital of Chengdu Medical College/Chengdu Pidu District People's HospitalChengdu 611730Sichuan ProvinceChina Department of Cardiology the Affiliated Third Hospital of Chengdu Traditional Chinese Medicine University/Chengdu Pidu District Hospital of Traditional Chinese MedicineChengdu 611730Sichuan ProvinceChina

BACKGROUND The MBOAT7 rs641738 single-nucleotide polymorphism(SNP)has been proven to influence various liver diseases,but its association with hepatocellular carcinoma(HCC)susceptibility has been *** address this discrepancy,we conducted the current systematic review and *** To perform a systematic review and meta-analysis on association of MBOAT7 SNP and HCC *** We performed a systematic review in PubMed,Web of Science,Scopus,and EMBASE;applied specific inclusion and exclusion criteria;and extracted the ***-analysis was conducted with the meta package in *** and subgroup analyses were also *** meta-analysis was registered in PROSPERO(CRD42023458046).RESULTS Eight studies were included in the systematic review,and 12 cohorts from 6 studies were included in the *** meta-analysis revealed an association between the MBOAT7 SNP and HCC susceptibility in both the dominant[odds ratio(OR):1.14,95%confidence interval(95%CI):1.02-1.26,P=0.020]and recessive(OR:1.21,95%CI:1.05-1.39,P=0.008)*** analysis revealed that stratification of the included patients by geographical origin showed a significant association in Asia(OR:1.20,95%CI:1.03-1.39).CONCLUSION This meta-analysis underscores the contribution of the MBOAT7 rs641738 SNP to hepatocarcinogenesis,especially in Asian populations,which warrants further investigation.

关键词： MBOAT7 Single-nucleotide polymorphisms Hepatocellular carcinoma Systematic review Meta-analysis Asian populations

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Coactivator-associated arginine methyltransferase 1: A versatile player in cell differentiation and development

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Genes & Diseases 2023年第6期10卷 2383-2392页

作者： Zhongrui Ma Xinxing Lyu Ning qin Haoyu Liu Mengrui Zhang Yongchao lai Bo Dong Peiyuan Lu Medical Research Center The First Affiliated Hospital of Shandong First Medical UniversityJinanShandong 250014China Department of Immunology Medical Science and Technology Innovation CenterShandong First Medical University&Shandong Academy of Medical SciencesJinanShandong 250117China Department of Biochemistry and Molecular Biology School of Basic MedicineShandong First Medical University&Shandong Academy of Medical SciencesJinanShandong 250117China Department of Cardiology Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinanShandong 250021China

Protein arginine methylation is a common post-translational modification involved in the regulation of various cellular functions. Coactivator-associated arginine methyltransferase 1 (CARM1) is a protein arginine methyltransferase that asymmetrically dimethylates histone H3 and non-histone proteins to regulate gene transcription. CARM1 has been found to play important roles in cell differentiation and development, cell cycle progression, autophagy, metabolism, pre-mRNA splicing and transportation, and DNA replication. In this review, we describe the molecular characteristics of CARM1 and summarize its roles in the regulation of cell differentiation and development in mammals.

关键词： Arginine methylation CARM1 Cell differentiation Development Epigenetic regulation

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In vivo genome editing thrives with diversified CRISPR technologies

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Zoological Research 2018年第2期39卷 58-71页

作者： Xun Ma Avery Sum-Yu Wong Hei-Yin Tam Samuel Yung-Kin Tsui Dittman lai-shun Chung Bo Feng Key Laboratory for Regenerative Medicine in Ministry of Education School of Biomedical Sciences Faculty of Medicine The Chinese University of Hong Kong Guangzhou Institute of Biomedicine and Health Chinese Academy of Sciences SBS Core Laboratory CUHK Shenzhen Research Institute

Prokaryotic type II adaptive immune systems have been developed into the versatile CRISPR technology, which has been widely applied in site- specific genome editing and has revolutionized biomedical research due to its superior efficiency and flexibility. Recent studies have greatly diversified CRISPR technologies by coupling it with various DNA repair mechanisms and targeting strategies. These new advances have significantly expanded the generation of genetically modified animal models, either by including species in which targeted genetic modification could not be achieved previously, or through introducing complex genetic modifications that take multiple steps and cost years to achieve using traditional methods. Herein, we review the recent developments and applications of CRISPR-based technology in generating various animal models, and discuss the everlasting impact of this new progress on biomedical research.

关键词： CRISPR/Cas9 Genome editing Animal models

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Facile Ionic Liquids-Promoted One-Pot Synthesis of Polyhydroquinoline Derivatives under Solvent Free Conditions

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有机化学 2004年第z1期24卷 257-258页

作者： JIANG,Zhao-qin LU,Jun ZHOU,Min-Feng JI,shun-Jun Jiang Provincial Key Laboratory of Fine Petrochemical Technology College of Chemistry and Chemical Engineering Suzhou University Suzhou 215006

In recent years, much attention has been directed towards the syntheses of 1,4-dihydropyridyl compounds due to the fact that 1,4-dihydropyridyl compounds possess a variety of biological activities.[1] In view of the importance of polyhydroquinoline derivatives, many classical methods for the synthesis of polyhydroquinoline derivatives were reported[2] by conventional heating and refluxing approaches in the presence of organic solvent. These methods, however, involve long reaction time, harsh reaction conditions, the use of a large quantity of organic solvent and unsatisfactory yields. Therefore,improvements in such syntheses have been sought continuously.……

关键词： organic solvent biological activities conventional heating reaction time methods for

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Carboxylesterase genes in nitenpyram-resistant brown planthoppers,Nilaparvata lugens

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Insect Science 2021年第4期28卷 1049-1060页

作者： Kaikai Mao Zhijie Ren Wenhao Li Tingwei Cai Xueying qin Hu Wan Byung Rae Jin shun He Jianhong Li Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina College of Natural Resources and Life Science Dong-A UniversityBusanRepublic of Korea

Carboxylesterases(CarEs)represent one of the major detoxification enzyme families involved in insecticide ***,the function of specific CarE genes in insecticide resistance is still unclear in the insect Nilaparvata lugens(Stål),a notorious rice crop pest in *** this study,a total of 29 putative CarE genes in *** were identified,and they were divided into seven clades;further,theβ-esterase clade was significantly ***-specific expression analysis found that 17 CarE genes were abundantly distributed in the midgut and fat body,while 12 CarE genes were highly expressed in the *** expression of most CarE genes was significantly induced in response to the challenge of nitenpyram,triflumezopyrim,chlorpyrifos,isoprocarb and *** these,the expression levels of NlCarE2,NlCarE4,NlCarE9,NlCarE17 and NlCarE24 were increased by each ***-time quantitative polymerase chain reaction and RNA interference assays revealed the NlCarE1 gene to be a candidate gene mainly involved in nitenpyram resistance,while simultaneously silencing NlCarE1 and NlCarE19 produced a stronger effect than silencing either one individually,suggesting a cooperative relationship in resistance *** findings lay the foundation for further clarification of insecticide resistance mediated by CarE in ***.

关键词： carboxylesterase expression profiling insecticide induction Nilaparvata lugens nitenpyram resistance

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