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molecular evolution of stress-response gene Leptin in high-altitude chinese snub-nosed monkeys (Rhinopithecus genus)

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chinese Science Bulletin 2010年第36期55卷 4132-4135页

作者： WANG XiaoPing1,2, JIN Wei1,2, YU Li1,2 & ZHANG YaPing1,2 1 laboratory for Conservation and Utilization of Bio-resource, Yunnan University, kunming 650091, china 2 State Key laboratory of Genetic Resources and evolution, kunming institute of zoology, chinese academy of sciences, kunming 650223. china laboratory for Conservation and Utilization of Bio-resource Yunnan University Kunming 650091 China State Key laboratory of Genetic Resources and evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China

chinese snub-nosed monkeys (genus Rhinopithecus, subfamily Colobinae), including R. bieti, R. brelichi and R. roxellana, are well-known as the non-human primates with the highest known altitudinal distribution. They represent an interesting model organism of adaptation to the extreme environmental stresses. However, no study at the molecular level has yet been reported for the high-altitude adaptation in chinese snub-nosed monkeys. Leptin, as an adipocyte-derived hormone, is believed to play an important role in energy homeostasis in adaptation to high altitude environments. In the present study, we sequenced and compared leptin sequences of the chinese snub-nosed monkeys (R. bieti and R. roxellana) with their lowland close relative R. avunculus and other Colobines. Unexpectedly, no amino acid changes were observed in the 7 Colobinae species examined, including the 2 chinese snub-nosed monkeys, indicating no difference in the evolutionary pattern of the Leptin gene between high-altitude monkeys and their lowland counterparts. In contrast to a previous finding of adaptive evolution of Leptin gene in plateau pikas, our study suggests that this gene may not have an important role in high-altitude adaptation of chinese snub-nosed monkeys. Other nuclear genes associated with energy metabolism, or mitochondrial genes, are most likely to be involved the molecular mechanism underlying adaptation of these monkeys to cold and hypoxia associated with the highland environment.

关键词： Leptin基因金丝猴属瘦素基因压力反应分子进化高空鼻极端环境

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酵母基因中转录正调控内含子序列特征的统计分析

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生物化学与生物物理进展 2003年第2期30卷 231-238页

作者：张静石秀凡云南大学应用统计中心昆明650091 中国科学院昆明动物研究所

大量实验研究显示 ,真核基因的许多内含子具有调控转录的功能 ,但是对此问题尚缺乏全面的研究 .观察一些酵母基因的转录频率与基因的内含子序列后 ,发现一个值得注意的现象 :转录频率高的基因内含子序列一般都比较长 ,而转录频率低的基... 详细信息

大量实验研究显示 ,真核基因的许多内含子具有调控转录的功能 ,但是对此问题尚缺乏全面的研究 .观察一些酵母基因的转录频率与基因的内含子序列后 ,发现一个值得注意的现象 :转录频率高的基因内含子序列一般都比较长 ,而转录频率低的基因内含子一般都比较短 .这提示高效转录基因的较长内含子中可能含有某些增强基因转录的特征性结构 .于是选取两组酵母基因的内含子进行详细研究 ,第一组的基因具有较高的转录频率 (>30 ) ,第二组的基因转录频率较低 (≤ 10 ) .对寡核苷酸 (主要是四核苷酸、五核苷酸 )的出现频率进行统计比较分析 ,探测到一批寡核苷酸 ,它们在第一组内含子中出现的频率显著高于在第二组内含子中出现的频率 ,同时也显著高于与第一组内含子相邻的外显子中的出现频率 .其中一些寡核苷酸与实验研究得到的转录调控元件相同 .从这批寡核苷酸在内含子和外显子序列中的分布看。

关键词：酵母基因转录正调控内含子序列特征基因转录频率寡核苷酸出现频率

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evolution of cd59 gene in mammals

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Science china(Life sciences) 2007年第6期50卷 773-779页

作者： GONG YuanYing1,2, PENG MinSheng1,2, ZHOU WeiPing1,3,4 & ZHANG YaPing1,4 1 State Key laboratory of Genetic Resources and evolution, kunming institute of zoology, chinese academy of sciences, kunming 650223. china 2 Graduate University of the chinese academy of sciences, Beijing 100049, china 3 molecular and Cell Biology, University of Science and Technology of china, Hefei 230026, china 4 laboratory for Conservation and Utilization of Bio-resources, Yunnan University, kunming 650091, china State Key laboratory of Genetic Resources evolution Kunming Institute of Zoology Chinese Academy of Sciences Kunming 650223 China Graduate University of the chinese academy of sciences Beijing 100049 China molecular Cell Biology University of Science Technology of china Hefei 230026 China laboratory for Conservation Utilization of Bio-resources Yunnan University Kunming 650091 China

The CD59-coding sequences were obtained from 5 mammals by PCR and BLAST, and combined with the available sequences in GenBank, the nucleotide substitution rates of mammalian cd59 were calcu- lated. Results of synonymous and nonsynonymous substitution rates revealed that cd59 experienced negative selection in mammals overall. Four sites experiencing positive selection were found by using "site-specific" model in PAML software. These sites were distributed on the molecular surface, of which 2 sites located in the key functional domain. Furthermore, "branch-site-specific" model detected 1 positive site in cd59a and cd59b lineages which underwent accelerated evolution caused by positive selection after gene duplication in mouse.

关键词： cd59, evolution, positive selection

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Lamins and their genes

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Progress in Natural Science:Materials International 2002年第8期 3-9页

作者： WEN Jianfan and CHEN Wanqun(1. Key laboratory of cellular and molecular evolution, kunming institute of zoology, chinese academy of sciences, kunming 650223. china 2. Graduate School of chinese academy of sciences, Beijing 100039, china) Key laboratory of cellular and molecular evolution，kunming institute of zoology，chinese academy of sciences，kunming 650223.china Key laboratory of cellular and molecular evolution，kunming institute of zoology，chinese academy of sciences，kunming 650223.china Graduate School of chinese academy of sciences，Beijing 100039，china

The nuclear lamina is an important nuclear structure. The studies on its peptides lamins and genes have made substantial progress in recent years. In this paper, the new achievements in studies of new lamin members and their functions, lamin genes and their origin and differentiation, are reviewed and discussed. Some research areas that deserve to be investigated further are put forward.

关键词： lamin lamin gene origin differentiation.

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苯丙氨酰-tRNA合成酶的进化与结构域丢失(英文)

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生物化学与生物物理学报 2003年第12期35卷 1061-1065页

作者：林军黄京飞中国科学院昆明动物研究所细胞与分子进化重点实验室中国科学院昆明动物研究所细胞与分子进化重点实验室昆明650223 中国科学院研究生院北京100039 云南省畜禽分子生物学重点实验室

基因的复制、融合以及基因的水平转移是许多蛋白质包括氨酰 tRNA合成酶 (aminoacyl tRNAsynthetase ,AARS)进化过程中的常见事件。然而作者研究的结果显示 ,苯丙氨酰 tRNA合成酶 (phenylalanyl tRNAsynthetase,PheRS)的进化主要表现为... 详细信息

基因的复制、融合以及基因的水平转移是许多蛋白质包括氨酰 tRNA合成酶 (aminoacyl tRNAsynthetase ,AARS)进化过程中的常见事件。然而作者研究的结果显示 ,苯丙氨酰 tRNA合成酶 (phenylalanyl tRNAsynthetase,PheRS)的进化主要表现为一些结构域的丢失 ;并且这种结构域的丢失不影响PheRS的功能或活性。通常在生物从细菌到真核生物的进化过程中 ,其基因组的大小和基因的数目都有所增加 ,然而有趣的是 ,真核生物中PheRS的结构域类型和数目都明显少于细菌的PheRS。PheRS通过结构域的丢失而进化的现象 ,似乎与某些AARS功能由多重专一性向单一专一性的演化有着“异曲同工”之妙。

关键词：苯丙氨酰-tRNA合成酶结构域丢失分子进化

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A preliminary molecular historical biogeography of Caragana (Leguminosae) based on ITS and trnL-F DNA sequence data

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Journal of Arid Land 2009年第1期1卷 64-73页

作者： MingLi ZHANG1,2, Yun KANG3 , JunBo YANG4 1 laboratory of evolution and Biodiversity Conservation in Arid Region, Xinjiang institute of Ecology and Geography, chinese academy of sciences, Urumqi 830011, china 2 institute of Botany, chinese academy of sciences, Beijing 100093, china 3 School of Pharmacy, Fudan University, Shanghai 200032, china 4 kunming institute of Botany, chinese academy of sciences, kunming 650204, china chinese Acad Sci Lab Evolut & Biodivers Conservat Arid Reg Xinjiang Inst Ecol & Geog Urumqi 830011 Peoples R China chinese Acad Sci Inst Bot Beijing 100093 Peoples R China Fudan Univ Sch Pharm Shanghai 200032 Peoples R China chinese Acad Sci Kunming Inst Bot Kunming 650204 Peoples R China

The nrDNA internal transcribed spacer (ITS) and cpDNA trnL-F internal spacer (IGS) sequence data of Caragana eight species and one outgroup Halimodendron halodendron, was employed to reconstruct a phylogenetic tree, then the area relationship was analyzed by means of component analysis (CA), Brooks parsimony analysis (BPA), and dispersal-vicariance analysis (DIVA), six areas were selected from two divided distributions of East Asia and Tethys in Caragana. The phylogenetic tree indicated that there were three distinctive groups, which were attributed to some morphological characters, first with pinnate foliage and deciduous rachis, second with palmate foliage and persistent sclerotic stick rachis, and third with pinnate foliage and persistent sclerotic stick rachis. The results of CA and BPA illustrated general area relationships. An explicit area relationship should be Altai-Sayan, Far East NE china and North china (Hengduan Mountains). DIVA recognized several explanatory vicariance and dispersal events. As the scenario of Caragana distribution pattern, it looks like the vicariance versus dispersal plays more important role. In vicaraince, there are not only the isolated far-distance vicariance, but also the adjacent vicariance especially a vicariance between Hengduan Mountains and North china.

关键词： Caragana ITS trnL-F phylogeny cladistic biogeography DIVA distribution pattern

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Frequency of the mtDNA 9-bp deletion in chinese ethnic groups

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Progress in Natural Science 2001年第5期11卷 40-46页

作者：姚永刚袁志刚周曾娣耿排力李庆伟张亚平 laboratory of cellular and molecular evolution kunming institute of zoology chinese academy of sciences kunming 650223 china Guangxi Medical University Nanning 530021 Yunnan Family Planning institute kunming 650021 Qinghai Medical College Xining 810001 Liaoning Normal University Dalian 116000

The 9-bp deletion in the COII/tRNALys intergenic region (region V) of human mitochondrial DNA was screened in 1521 chinese from 16 ethnic groups and 9 Han geographic groups. The highest frequency was found in populations of Miao (32.4%) and Bouyei (30.8%) from Cuizhou Province, whereas no deletion was found in Kazak population in Xinjiang. In the populations of Wa and Lahu from Yunnan Province, Uygur and Mongolian from Xinjiang, the deletion frequency was relatively low (≤4%), while in the remaining 18 groups, the frequency was moderate (6% - 24 %). Except those Hans in Xinjiang, Guizhou and that reported in Taiwan, the deletion frequency in the Han geographic groups did not show a substantial difference. However, the deletion frequency in some ethnic groups from the same geographic region or with similar ethnohistory did not show similarity. A general decrease tendency in the deletion frequency was found from south to north and from coastal to inland. The frequency of the 9-bp deletion was approximate

关键词： mtDNA chinese ethnic group 9-bp deletion.

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Yeast基因下游二级结构与多聚腺苷作用信号

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生物化学与生物物理进展 2001年第4期28卷 523-527页

作者：张静石秀凡刘次全云南大学应用统计中心昆明650091 中国科学院昆明动物研究所昆明650223 云南大学现代生物中心昆明650091

形成真核生物mRNA 3′末端的多聚腺苷 (poly (A) )作用涉及前体mRNA下游的三个元件 :效率元件(EE)、定位元件 (PE)以及实际的剪切和 poly (A)作用位点 ,实验研究提出了一些EE和PE的碱基序列组成 .对 180个Yeast基因下游 (终止密码子后 2... 详细信息

形成真核生物mRNA 3′末端的多聚腺苷 (poly (A) )作用涉及前体mRNA下游的三个元件 :效率元件(EE)、定位元件 (PE)以及实际的剪切和 poly (A)作用位点 ,实验研究提出了一些EE和PE的碱基序列组成 .对 180个Yeast基因下游 (终止密码子后 2 0 0个碱基 )二级结构进行的详细分析显示 ,约 86 %的EE、 89%的PE与二级结构中碱基非配对的环 (发夹环、膨胀环、内环或多分支环 )区或连接单链区有关 .这个结果提示 ,反式因子对EE和PE的识别和作用在一定程度上有赖于EE和PE的二级结构特征 .

关键词： Yeast基因多聚腺苷作用信号 mRNA二级结构蛋白质合成

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Down-regulated expression of atypical PKC-binding domain deleted asip isoforms in human hepatocellular carcinomas

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Cell Research 2001年第3期11卷 223-229页

作者： FANG CHANG MINC, YONG HUA XU laboratory of molecular and cellular Oncology, institute of Biochemistry and Cell Biology, Shanghai institutes for Biological sciences, chinese academy of sciences, Shanghai 200031, china 1laboratory of molecular and cellular Oncology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China. 1laboratory of molecular and cellular Oncology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China.

Asip is a mammalian homologue of polarity protein Par-3 of Caenorhabditis elegans and Bazooka of Drosophila melanogaster. Asip/Par-3/Bazooka are PDZ-motif containing proteins that localize asymmetrically to the cell periphery and play a pivotal role in cell polarity and asymmetric cell division. In the present study, we have cloned human asip cDNA and its splicing variants by 5’-RACE and RT-PCR using candidate human EST clones which have a high homology to rat asip cDNA. The full-length cDNA of human asip encodes a 1,353 aa protein exhibiting 88% similarity to the rat one. Human asip is a single copy gene consisting of at least 26 exons and localizing in human chromosome 10, band p11.2, with some extraordinarily long introns. All exon/intron boundary nucleotides conform to the "gt-ag" rule. Three main transcripts were detected by Northern blot analysis, and at least five variants, from alternative splicing and polyadenylation, have been identified by RT-PCR and liver cDNA library screening. Exon 17b deleted asip mRNAs expressed ubiquitously in normal human tissues, including liver, on RT-PCR analysis. However, they were absent from most human liver cancer cell lines examined. More interestingly, the expression of exon 1 7b deleted variants was down regulated in 52.6% (10/19) clinic specimens of human hepatocellular carcinomas (HCCs), compared with the surrounding nontumorous liver tissues from the same patients. The presence of various splicing transcripts, the variation of their distribution among different tissues and cells, and their differential expressions in human HCCs suggest that human Asip isoforms may function in different context.

关键词： Alternative splicing and polyadenylation A sip/Par-3/Bazooka PDZ-motif protein Polarity protein hepatocellular carcinomas.

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Does the ’Anucleolate’ Giardia Have a Ribosomebiogenesis System Typical to Eukaryotic?

Does the ’Anucleolate’ Giardia Have a Ribosomebiogenesis S...

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12th International Congress of Protozoology

作者： Dedong Xin Jun Sun Jianfan Wen Key laboratory of cellular and molecular evolution kunming institute of zoology chinese academy of sciences kunming Yunnan 650223.P. R. china

＜正＞Nucleolus is the most prominent feature in the interphase nuclei of the eukaryotic cells. The nucleolus is organized around the tendemly repeated genes of ribosomal RNA. Being a subcellular organelle without membrane, nucleolus is the site of ribosomal RNA gene transcription and ribosomal subunits assembly. Giardia lamblia is medically important as a cause of diarrhea and malabsorption throughout the world. It is also an important organism for the evolutionary studies. Previous reports of others and ours revealed that Giardia does not have a nucleolus structure. Then, is there any difference of the mechanism of ribosome biogenesis between Giardia and other typical eukaryotes?

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