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Effects of N-acetylcysteine on growth,viability and reactive oxygen species levels in small antral follicles cultured in vitro

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Asian pacific Journal of reproduction 2023年第1期12卷 42-48页

作者： Efigênia B.Cordeiro Bianca r.silva laís r.f.m.paulino Pedro A.A.Barroso laryssa G.Barrozo miguel f.de Lima Neto Josér.V.silva laboratory of Biotechnology and Physiology of reproduction Federal University of CearaAv.Comandante Maurocélio Rocha Ponte 10062041-040SobralCEBrazil

Objective:To investigate the effects of different concentrations of N-acetylcysteine on follicular growth and morphology,as well as on viability,levels of reactive oxygen species(rOs)and meiotic progression of oocytes from in vitro cultured bovine early antral ***:Isolated early antral follicles(about 500μm)were cultured in TCm-199+alone or supplemented with 1.0,5.0 or 25.0 mm N-acetylcysteine at 38.5℃with 5%CO_(2) for 8 *** diameters were evaluated at day 0,4 and 8 of *** the end of culture,the levels of rOs,chromatin configuration and viability(calcein-Am and ethidium homodimer-1 staining)were investigated in the cumulus-oocyte *** of follicle diameters between treatments were *** on percentages of morphologically normal follicles,growth rates and chromatin configuration in different treatments were ***:An increase in follicular diameters after culture in all treatments was observed,except for follicles cultured with 25.0 mm *** microscopy showed that oocytes cultured in all treatments were stained positively with calcein Am,and that 5.0 mm N-acetylcysteine reduced fluorescence for ethidium *** levels of rOs in oocytes from follicles cultured with 1.0 mm N-acetylcysteine showed a significant reduction compared to other *** presence of N-acetylcysteine in culture medium did not influence the rates of oocyte at the germinal vesicle ***:N-acetylcysteine at concentrations of 1.0 and 5.0 mm reduces rOs levels and staining for ethidium homodimer-1 in in vitro cultured follicles,respectively,while 25.0 mm N-acetylcysteine decreases follicular growth and the percentages of continuously growing follicles.

关键词： Antioxidant N-acetylcysteine Antral follicles Bovine reactive oxygen species

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Effect of biochar on the metabolome of soybean seedlings

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Oil Crop science 2024年第1期9卷 60-68页

作者： Nathalia E.silva mariana G.Aguilar Osania E.ferreira Gleicia m.paulino Jaqueline C.L.Carvalho Lúcia P.s.Pimenta Alan r.T.machado Programa de Pos-graduaçao em Ciencias Ambientais Universidade do Estado de Minas GeraisFrutalMinas Gerais38202-436Brazil Departamento de Química Universidade Federal de Minas GeraisMinas GeraisBelo Horizonte31270-901Brazil Departamento de Engenharia Aplicada e Tecnologias Ambientais Universidade do Estado de Minas GeraisJoao MonlevadeMinas Gerais35930-314Brazil Departamento de Ciencias Exatas Universidade do Estado de Minas GeraisJoao MonlevadeMinas Gerais35930-314Brazil

The use of biochar can have several effects on plant germination,depending on raw material,preparation method and application ***,the molecular mechanisms that lead to those results have yet to be *** aim of this research was to improve the understanding of these mechanisms by characterizing the metabolic effects of sugarcane bagasse biochar on soybean *** types of biochars were prepared by pyrolysis at 300℃(sCB300),400℃(sCB400)and 600℃(sCB600).Then,each one was mixed into sand at 1%,3%,5%(w/w)dose,*** experiment was performed in 8 days of incubation,when the number of germinated seeds and the average radicle length were *** evaluate the metabolome,the dry biomass(DB)was subjected to extraction with a mixture of methanol-d4 and D2O(1:1 v/v).The extracts were submitted to metabolomics analysis by Proton Nuclear magnetic *** relative Germination,relative Average radicle Growth and Germination Index increased in all treatments compared to *** the other hand,the DB increased in all treatments,except for sCB300,at doses of 1%and 3%w/*** metabolites(alanine,asparagine,acetic acid,citric acid,glycerol,fatty acids and sucrose)were identified and quantified in DB extracts as the most influential finding for the separation of *** together,these results strongly suggested that biochars accelerated the catabolism of triacylglycerols to sucrose and induced a slight osmotic stress.

关键词： Germination metabolomics 1H Nmr Pyrolysis sugarcane bagasse

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Effect of solidification cooling rate on microstructure and tribology characteristics of Zn-4si alloy

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International Journal of minerals,metallurgy and materials 2024年第2期31卷 362-373页

作者： f.Akbari m.Golkaram s.Beyrami G.shirazi K.mantashloo r.Taghiabadi m.saghafi Yazdi I.Ansarian Department of metallurgy and material science Imam Khomeini International University(IKIU)Qazvin ***Iran

The main objective of this work was to modify the microstructure and enhance the tribological properties of a new Zn-4si al-loy through a high solidification cooling rate(sCr).According to the results,by increasing the sCr from 2.0 to 59.5℃/s the average size of primary si particles and that of the grains reduced from 76.1 and 3780μm to less than about 14.6 and 460μm,***-ing the sCr also enhanced the microstructural homogeneity,decreased the porosity content(by 50%),and increased the matrix hardness(by 36%).These microstructural changes enhanced the tribological *** instance,under the applied pressure of 0.5 mPa,an in-crease in the sCr from 2.0 to 59.5℃/s decreased the wear rate and the average friction coefficient of the alloy by 57%and 23%,*** wear mechanism was also changed from the severe delamination,adhesion,and abrasion in the slowly-cooled alloy to the mild tribolayer delamination/abrasion in the high-cooling-rate-solidified sample.

关键词： zinc-silicon alloy primary silicon solidification cooling rate tribology sliding wear

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fast efficient photon deceleration in plasmas by using two laser pulses at different frequencies

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matter and radiation at Extremes 2024年第3期9卷 11-18页

作者： Y.X.Wang X.L.Zhu s.m.Weng P.Li X.f.Li H.Ai H.r.Pan Z.m.sheng Key laboratory for laser Plasmas(moe) School of Physics and AstronomyShanghai Jiao Tong UniversityShanghai 200240China Collaborative Innovation Center of IfsA Shanghai Jiao Tong UniversityShanghai 200240China research Center of laser fusion of China Academy of Engineering Physics MianyangSichuan 621900China state key laboratory of High field laser Physics Shanghai Institute of Optics and Fine MechanicsChinese Academy of SciencesShanghai 201800China Tsung-dao Lee Institute Shanghai Jiao Tong UniversityShanghai 200240China

The generation of ultrashort high-power light sources in the mid-infrared(mid-Ir)to terahertz(THz)range is of interest for applications in a number of fields,from fundamental research to biology and *** conventional laser technology,photon deceleration in plasma wakes provides an alternative approach to the generation of ultrashort mid-Ir or THz ***,we present a photon deceleration scheme for the efficient generation of ultrashort mid-Ir or THz pulses by using an intense driver laser pulse with a relatively short wavelength and a signal laser pulse with a relatively long *** signal pulse trails the driver pulse with an appropriate time delay such that it sits at the front of the second wake bubble that is driven by the driver *** to its relatively long wavelength,the signal pulse will be subjected to a large gradient of the refractive index in the plasma wake ***,the photon deceleration in the plasma wake becomes faster and more efficient for signal pulses with longer *** greatly enhances the capacity and efficiency of photon deceleration in the generation of ultrashort high-power light sources in the long-wavelength Ir and THz spectral ranges.

关键词： laser ultrashort photon

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Comparative analysis of microstructure,mechanical,and corrosion properties of biodegradable mg-3Y alloy prepared by selective laser melting and spark plasma sintering

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Journal of magnesium and Alloys 2024年第4期12卷 1496-1510页

作者： P.minárik m.Zemková s.Šašek J.Dittrich m.Knapek f.Lukáˇc D.Koutný J.Jaroš r.Král faculty of mathematics and Physics Charles UniversityKe Karlovu 5Praha 12116Czech Republic research Centre University ofŽilinaUniverzitná8215/1Žilina 01026Slovakia Institute of Plasma Physics of the Czech Academy of sciences Za Slovankou 1782/318200 Praha 8Czech Republic Institute of machine and Industrial Design Brno University of TechnologyTechnická2896/2Brno 61669Czech Republic

This work explored possibilities of biodegradable magnesium alloy mg-3Y preparation by two modern powder metallurgy techniques–spark plasma sintering(sPs)and selective laser melting(sLm).The powder material was consolidated by both methods utilising optimised parameters,which led to very low porosity(∼0.3%)in the sLm material and unmeasurably low porosity in the sPs *** main aim of the study was the thorough microstructure characterisation and interrelation between the microstructure and the functional properties,such as mechanical strength,deformability,and corrosion *** materials showed comparable strength of∼110 mPa in tension and compression and relatively good deformability of∼9%and∼21%for the sLm and sPs materials,*** corrosion resistance of the sPs material in 0.1 m NaCl solution was superior to the sLm one and comparable to the conventional extruded *** digital image correlation during loading and the cross-section analysis of the corrosion layers revealed that the residual porosity and large strained grains have the dominant negative effect on the functional properties of the sLm *** the other hand,one of the primary outcomes of this study is that the sPs consolidation method is very effective in the preparation of the W3 biodegradable alloy,resulting in material with convenient mechanical and degradation properties that might find practical applications.

关键词： magnesium Yttrium Powder metallurgy microstructure mechanical strength Corrosion resistance

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One-neutron stripping process in the^(209)Bi(^(6)Li,^(5)Li)^(210)Bi*reaction reaction

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Nuclear science and Techniques 2024年第6期35卷 120-129页

作者： Gao-Long Zhang Zhen-Wei Jiao Guang-Xin Zhang E.N.Cardozo B.Paes shi-Peng Hu Jian-Qiang Qian Daniele mengoni Wei-Wei Qu Cong-Bo Li Yun Zheng Huan-Qiao Zhang Hui-Bin sun Nan Wang Chun-Lei Zhang J.J.Valiente-Dobón D.Testov m.mazzocco A.Gozzelino C.Parascandolo D.Pierroutsakou m.la Commara A.Goasduff D.Bazzacco D.r.Napoli f.Galtarossa f.recchia A.Illana s.Bakes I.Zanon s.Aydin Gde Angelis m.siciliano r.menegazzo s.m.Lenzi s.Akkoyun L.f.Canto J.Lubian school of Physics Beihang UniversityBeijing 100191China sino-french Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China Instituto de física Universidade Federal Fluminense24210-340 NiteróiRio de JaneiroBrazil laboratorio TANDAr Comisión Nacional de Energía AtómicaBKNA1650 San MartíınArgentina Institute for Advanced study in Nuclear Energy and safety Shenzhen UniversityShenzhen 518060China shenzhen Key laboratory of research and manufacture of High Purity Germanium materials and fetectors Shenzhen UniversityShenzhen 518060China INfN Sezione di PadovaPaduaItaly Dipartimento di fisica Astronomia dell’Universita di padova PaduaItaly state Key laboratory of radiation medicine and Protection School of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China China Institute of Atomic Energy Beijing 102413China The Key laboratory of Beam Technology and material modification of ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China laboratori Nazionali di Legnaro INFN35020 LegnaroItaly INfN Sezione di Napoli80126 NaplesItaly Dipartimento di farmacia Universitàdi Napoli“Federico II”80131 NaplesItaly Department of Natural and mathematical sciences Faculty of EngineeringTarsus University33480 MersinTurkey Physics Division Argonne National LaboratoryLemontILUSA Department of Physics Sivas Cumhuriyet UniversitySivasTurkey Instituto de física Universidade Federal do Rio de JaneiroRio de Janeiro CP 6852821941-972Brazil

One-neutron stripping process between^(6)Li and^(209)Bi was studied at 28,30,and 34 meV using the in-beamγ-ray spectroscopy ***γ-γcoincident analysis clearly identified twoγ-rays feeding the ground and long-lived isomeric states,which were employed to determine the cross *** one-neutron stripping cross sections were similar to the cross sections of complete fusion in the^(6)Li+^(209)Bi system,but the one-neutron stripping cross sections decreased more gradually at the sub-barrier region.A coupled-reaction-channel calculation was performed to study the detailed reaction mechanism of the one-neutron stripping process in^(6)*** calculations indicated that the first excited state of 5 Li is critical in the actual one-neutron transfer mechanism,and the valence proton of 209Bi can be excited to the low-lying excited state in(^(6)Li,^(5)Li)reaction,unlike in the(d,p)reaction.

关键词： Transfer reaction Weakly bound nuclei Cross section

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further investigation on the fusion of ^(6)Li with ^(209)Bi target at near-barrier energies

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Chinese Physics C 2024年第7期48卷 106-117页

作者：张高龙焦振威张广鑫 m.r.Cortes 胡世鹏钱建强 D.mengoni 屈卫卫李聪博郑云张焕乔孙慧斌王楠张春雷 J.J.Valiente-Dobón D.Testov m.mazzocco A.Gozzelino C.Parascandolo D.Pierroutsakou m.la Commara A.Goasduff D.Bazzacco D.r.Napoli f.Galtarossa f.recchia A.Illana s.Bakes I.Zanon s.Aydin G.de Angelis m.siciliano r.menegazzo s.m.Lenzi J.L.ferreira J.rangel serkan Akkoyun L.f.Canto J.Lubian school of Physics Beihang UniversityBeijing 100191China sino-french Institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China Instituto de física Universidade Federal Fluminense24210-340NiteróiRio de JaneiroBrazil Institute for Advanced study in Nuclear Energy&safety Shenzhen UniversityShenzhen 518060China shenzhen Key laboratory of research and manufacture of High Purity Germanium materials and fetectors Shenzhen UniversityShenzhen 518060China INfN Sezione di PadovaPadovaItaly Dipartimento di fisica Astronomia dell'Universita di padova PadovaItaly state Key laboratory of radiation medicine and Protection School of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China China Institute of Atomic Energy Beijing 102413China The Key laboratory of Beam Technology and material modification of ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China INfN Laboratori Nazionali di LegnaroI-35020 LegnaroItaly INfN Sezione di NapoliI-80126 NapoliItaly Dipartimento di farmacia Universitàdi Napoli"Federico II"I-80131 NapoliItaly Department of Natural and mathematical sciences Faculty of EngineeringTarsus University33480 MersinTurkiye Physics Division Argonne National LaboratoryLemont-ILUSA Departamento de matemaética Física e Computaç˜ao Universidade do Estado do Rio de JaneiroFaculdade de Tecnologia27537-000ResendeRio de JaneiroBrazil Department of Physics Sivas Cumhuriyet UniversitySivasTurkey Instituto de física Universidade Federal do Rio de JaneiroCP 6852821941-972Rio de JaneiroBrazil

The complete and incomplete fusion cross sections for ^(6)Li+^(209)Bi were measured using the in-beamγ-ray method around the Coulomb *** cross sections of(deuteron captured)incomplete fusion(ICf)products were re-quantified experimentally for this reaction *** results reveal that the ICf cross section is equivalent to that of complete fusion(Cf)above the Coulomb barrier and dominant near or below the Coulomb barrier.A theoretical calculation based on the continuum discretized coupled channel(CDCC)method was performed for the aforementioned Cf and ICf cross sections;the result is consistent with the *** universal fusion function(Uff)was also compared with the measured Cf cross section for different barrier parameters,demonstrating that the Cf suppression factor is significantly influenced by the choice of potential,which can reflect both dynamic and static effects of breakup on the fusion process.

关键词： cross section incomplete fusion suppression factor weakly bound nuclei

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mgO-attached graphene nanosheet(mgO@GNs)reinforced magnesium matrix nanocomposite with superior mechanical,corrosion and biological performance

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International Journal of minerals,metallurgy and materials 2024年第9期31卷 2062-2076页

作者： s.Abazari A.shamsipur H.r.Bakhsheshi-rad m.s.soheilirad f.Khorashadizade s.s.mirhosseini Department of materials and metallurgical Engineering Amirkabir University of TechnologyTehran ***Iran Iranian National standards Organization of Hormozgan HormozganIran Advanced materials research Center Department of Materials EngineeringNajafabad BranchIslamic Azad UniversityNajafabadIran Department of materials and metallurgical Engineering Iran University of Science and TechnologyTehranIran

magnesium(mg)alloys are gaining great consideration as body implant materials due to their high biodegradability and ***,they suffer from low corrosion resistance and antibacterial *** this research,semi-powder metallurgy followed by hot extrusion was utilized to produce the magnesium oxide@graphene nanosheets/magnesium(mgO@GNs/mg)composite to improve mechanical,corrosion and cytocompatibility *** have revealed that the incorporation of mgO@GNs nanohybrids into mg-based composite enhanced microhardness and compressive *** vitro,osteoblast cell culture tests show that using mgO@GNs nanohybrid fillers enhances osteoblast adhesion and apatite *** presence of mgO@GNs nanoparticles in the composites decreased the opening defects,micro-cracks and micro-pores of the composites thus preventing the penetration of the corrosive solution into the *** demonstrated that the mgO@GNs/mg composite possesses excellent antibacterial properties because of the combination of the release of mgO and physical damage to bacterium membranes caused by the sharp edges of graphene nanosheets that can effectively damage the cell wall thereby facilitating penetration into the bacterial lipid ***,the mgO@GNs/mg composite with high mechanical strength,antibacterial activity and corrosion resistance is considered to be a promising material for load-bearing implant applications.

关键词： metal matrix composites mgO@GNs nanohybrid strengthening mechanisms antibacterial activity biocompatibility

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The viscous strip approach to simplify the calculation of the surface acoustic wave generated streaming

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Applied mathematics and mechanics(English Edition) 2024年第4期45卷 711-724页

作者： f.JAZINI DOrCHEH m.GHAssEmI fuel Cells and Nano systems(fCNs)laboratory Department of Mechanical EngineeringK.N.Toosi University of TechnologyTehran 19697Iran

In recent decades,the importance of surface acoustic waves,as a biocompatible tool to integrate with microfluidics,has been proven in various medical and biological *** numerical modeling of acoustic streaming caused by surface acoustic waves in microchannels requires the effect of viscosity to be considered in the equations which complicates the *** this paper,it is shown that the major contribution of viscosity and the horizontal component of actuation is concentrated in a narrow region alongside the actuation *** the inviscid equations are considerably easier to solve,a division into the viscous and inviscid domains would alleviate the computational load *** particles'traces calculated by this approximation are excellently alongside their counterparts from the completely viscous *** is also shown that the optimum thickness for the viscous strip is about 9-fold the acoustic boundary layer thickness for various flow patterns and amplitudes of actuation.

关键词： surface acoustic wave microfluidics numerical simulation particle tracing acoustic streaming

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radioisotope production using lasers:from basic science to applications

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matter and radiation at Extremes 2024年第3期9卷 31-44页

作者： m.r.D.rodrigues A.Bonasera m.scisciò J.A.Pérez-Hernández m.Ehret f.filippi P.L.Andreoli m.Huault H.larreur D.singappuli D.molloy D.raffestin m.Alonzo G.G.rapisarda D.lattuada G.L.Guardo C.Verona fe.Consoli G.Petringa A.mcNamee m.la Cognata s.Palmerini T.Carriere m.Cipriani G.Di Giorgio G.Cristofari r.De Angelis G.A.P.Cirrone D.margarone L.Giuffrida D.Batani P.Nicolai K.Batani r.Lera L.Volpe D.Giulietti s.Agarwal m.Krupka s.singh fa.Consoli Cyclotron Institute Texas A&M UniversityCollege StationTexas 77840USA laboratori Nazionali del sud Istituto Nazionale di Fisica Nucleare(LNS-INFN)CataniaItaly ENEA Fusion and Technologies for Nuclear Safety and Security Department-CFrascatiItaly Centro de Láseres Pulsados(clpu) VillamayorSpain Universidad de salamanca SalamancaSpain Centre lasers Intenses et Applications(CELIA) Universitéde BordeauxCNRSCEATalenceFrance HB11 Energy Holdings Pty FreshwaterNew South WalesAustralia school of mathematics and Physics Queen’s University BelfastBelfastUnited Kingdom Dipartimento di fisica e Astronomia“E.majorana ”Università di CataniaCataniaItaly facoltàdi Ingegneria e Architettura Universitàdegli Studi di Enna“Kore”EnnaItaly Dipartimento di Ingegneria Industriale Universitàdi Roma“Tor Vergata”RomaItaly Dipartimento di fisica e Geologia Universitàdegli Studi di PerugiaPerugiaItaly Istituto Nazionale di fisica Nucleare Sezione di PerugiaPerugiaItaly ELI Beamlines facility the Extreme Light Infrastructure ERICDolni BrezanyCzech Republic ETsIA Universidad Politécnica de MadridMadridSpain Dipartimento fisica “E.Fermi”Universitàdi Pisa and INFNPisaItaly fZU–Institute of Physics of Czech Academy of sciences PragueCzech Republic Institute of Plasma Physics of Czech Academy of sciences PragueCzech Republic

The discovery of chirped pulse amplification has led to great improvements in laser technology,enabling energetic laser beams to be compressed to pulse durations of tens of femtoseconds and focused to a few *** with energies of tens of meV can be accelerated using,for instance,target normal sheath acceleration and focused on secondary *** such conditions,nuclear reactions can occur,with the production of radioisotopes suitable for medical *** use of high-repetition lasers to produce such isotopes is competitive with conventional methods mostly based on *** this paper,we study the production of^(67)Cu,^(63)Zn,^(18)f,and^(11)C,which are currently used in positron emission tomography and other *** the same time,we study the reactions^(10)B(p,α)^(7)Be and^(70)Zn(p,4n)^(67)Ga to put further constraints on the proton distributions at different angles,as well as the reaction^(11)B(p,α)^(8)Be relevant for energy *** experiment was performed at the 1 PW laser facility at VegaⅢin salamanca,*** distributions of radioisotopes in the forward(with respect to the laser direction)and backward directions were measured using a high purity germanium *** results are in reasonable agreement with numerical estimates obtained following the approach of Kimura and Bonasera[*** ***.,sect.A 637,164–170(2011)].

关键词： purity estimates backward

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