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In vitro induced pluripotency from urine-derived cells in porcine

World Journal of stem Cells 2022年第3期14卷 231-244页

作者： Kaiana Recchia Lucas simões Machado Ramon Cesar Botigelli Naira Caroline Godoy Pieri Gabriela Barbosa Raquel Vasconcelos Guimarães de Castro Mariana Groke Marques laís vicari de figueiredo pessôa Paulo Fantinato Neto Flávio Vieira Meirelles Aline Fernanda de souza simone Maria Massami Kitamura Martins Fabiana Fernandes Bressan department of surgery Faculty of Veterinary Medicine and Animal SciencesUniversity of São PauloPirassununga 13635-000São PauloBrazil department of Pharmacology and Biotechnology Institute of BioscienceSão Paulo State UniversityBotucatu 18618-689São PauloBrazil department of Veterinary Medicine Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil Embrapa suínos e Aves Empresa Brasileira de Pesquisa AgropecuáriaConcordia 89715-899Santa CatarinaBrazil department of Animal sciences Faculty of Animal Sciences and Food EngineeringUniversity of São PauloPirassununga 13635-000São PauloBrazil

BACKGROUND The generation of induced pluripotent stem cells(iPsC)has been a game-changer in translational and regenerative medicine;however,their large-scale applicability is still hampered by the scarcity of accessible,safe,and reproducible *** porcine model is a large biomedical model that enables translational applications,including gene editing,long term in vivo and offspring analysis;therefore,suitable for both medicine and animal *** To reprogramme in vitro into pluripotency,and herein urine-derived cells(UDCs)were isolated from porcine *** The UDCs were reprogrammed in vitro using human or murine octamer-binding transcription factor 4(OCT4),sRY-box2(sOX2),Kruppel-like factor 4(KLF4),and C-MYC,and cultured with basic fibroblast growth factor(bFGF)*** characterize the putative porcine iPsCs three clonal lineages were submitted to immunocytochemistry for alkaline phosphatase(AP),OCT4,sOX2,NANOG,TRA181 and ssEA 1 *** transcripts related to the pluripotency(OCT4,sOX2 and NANOG)were analyzed via reverse transcription quantitative realtime polymerase chain reaction in different time points during the culture,and all three lineages formed embryoid bodies(EBs)when cultured in suspension without bFGF *** The UDCs were isolated from swine urine samples and when at passage 2 submitted to in vitro *** of putative iPsCs were obtained only from UDCs transduced with the murine factors(mOsKM),but not from human factors(hOsKM).Three clonal lineages were isolated and further cultured for at least 28 passages,all the lineages were positive for AP detection,the OCT4,sOX2,NANOG markers,albeit the immunocytochemical analysis also revealed heterogeneous phenotypic profiles among lineages and passages for NANOG and ssEA1,similar results were observed in the abundance of the endogenous transcripts related to pluripotent *** the clonal lineages when cultured in suspension without

关键词： Induced pluripotent stem cells Noninvasive Pluripotency Reprogramming Urine Porcine

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矿渣固化泥浆水泥塞的可钻性和钻头性能评价

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国外钻井技术 1995年第6期10卷 57-63页

作者： pess.,RC 吴达华

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不同页岩要求采用不同的钻井对策

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国外钻井技术 1996年第3期11卷 13-16,44页

作者： pess.,RC 袁永嵩

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Ab Initio study on the Reaction of la￣+ + CH_4→la+-CH￣2 + H_2

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Chinese Chemical Letters 1996年第11期7卷 1043-1046页

作者： Zhu R.s. Dai s.s. department of Chemistry Yunnan University Kunming 650091

Ab initio study on the reaction or the ground state (a3F) and excited state (a1D) or la+with methane has been Performed. performed. channel on the singlet and triplet potential energysurface (PEs) has been examined and the reaction mechanism are discussed. Comparisons betweensinglet and triplet pess show that the ex￣ state (aiD) of la+ is more r￣ than its groundstate (a3F) due tO the sPin quantum number conservation with the more stable insertion intermediate for excited state (1D), which is in good agreement with the experiment.

关键词： CH~2 Ab Initio study on the Reaction of la H2

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Ab initio studies on the Reaction of M￣++CH_4→M￣+CH_2+H_2(M=sc,Y,la)

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Chemical Research in Chinese Universities 1996年第4期12卷 368-373页

作者： Zhu, Rs Dai, ss Huang, JH Ye, s YUNNAN UNIV DEPT CHEMKUNMING 650091PEOPLES R CHINA

The present paper covers the reactions of M￣+ +CH_4→M￣+CH_2+H_2(M=sc,Y,la)investigated theoretically using ab initio MO methods and reaction channels onthe singlet potential energy surfaces (pess) examined and discusses the reactionmechanism. The results indicate that the formation of M￣+CH_2 is primarily via a sin-glet H M￣+ CH_3 intermediate,followed by a four-center elimination of H_2,all ofthese reactions have negative activation energies and the trend of reactivity is la￣+>sc￣+>Y￣+.

关键词： s:Ab initio calculation Transition metalion Methane Reaction mechaNism

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Direct ab initio dynamics study of radical C4H（Χ~2 Σ+）+ CH4 reaction

Direct ab initio dynamics study of radical C4H（Χ~2 Σ+）+ ...

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中国化学会第28届学术年会

作者： Rui-Ping Huo,Xiang Zhang,Xu-Ri Huang,Ji-lai Li,Chia-Chung sun state Key laboratory of Theoretical and Computational Chemistry,Institute of Theoretical Chemistry,Jilin University,Changchun 130023,People''s Republic of China school of Chemistry and Materials science,shanxi Normal University,Linfen 041004,People''s Republic of China

The methane（CH4）hydrogen abstraction reaction by linear butadiynyl radical C4H（CCCCH）has been investigated by direct ab initio dynamics over a wide temperature range 100-3000K *** potential energy surfaces（pess）have been constructed at the CCsD（T）/aug-cc-pVTZ//BB1K/6-311G（d,p）levels of *** different hydrogen abstraction channels by C1 and C4 of C4H（C1C2C3C4H）have been *** results indicate that C1 position of C4H is a more reactive *** electron transfer behaviors of two possible channels are also analyzed by quasi-restricted orbital（QRO）in *** rate constants calculated by canonical variational transition-state theory（CVT）with the small-curvature tunneling correction（sCT）are in excellent agreement with available experimental *** normal and three-parameter expressions of Arrhenius rate constants are also provided within *** is expected to be helpful for further studies on the reaction dynamics behaviors over a wide temperature range where no experimental data is available so far.

关键词： C4H CH4 rate constants VTsT temperature dependence

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