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Phosphorylation of Cofilin-1 Enhances Paclitaxel Resistance of Epithelial Ovarian Cancer Cells by Inhibiting Apoptosis

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Biomedical and Environmental Sciences 2021年第6期34卷 465-477页

作者： LI Min DONG Xu Dong lyu qiu bo ZHANG Wei HUANG Shuai YANG Chun Xue CUI Di LAI Hui Ying Department of Obstetrics and Gynecology Beijing Hospital National Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Department of Obstetrics the First People's Hospital of Yunnan ProvinceKunming 650032YunnanChina Department of Pathology Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China Peking University Health Science Center Beijing 100069China Clinical Laboratory Beijing HospitalNational Center of GerontologyInstitute of Geriatric MedicineChinese Academy of Medical SciencesBeijing 100730China

Objective To investigate the molecular mechanism of high phosphorylation levels of cofilin-1(p-CFL-1)associated with paclitaxel resistance in epithelial ovarian cancer(EOC)*** Cells displaying varying levels of p-CFL-1 and CFL-1 were created by plasmid transfection and shRNA *** inhibition rate indicating paclitaxel efficacy was assessed by Cell Counting Kit-8(CCK-8)*** was assessed by flow cytometry and protein levels were detected by western *** real-time polymerase chain reaction(qRT-PCR)was used to measure the expression levels of phosphokinases and phosphatases of *** analysis evaluated the correlation between the prognosis of EOC patients and the levels of p-CFL-1 and slingshot-1(SSH-1).Results High levels of p-CFL-1 were observed in EOC cells that survived treatment with high doses of ***3 cell mutants with upregulated p-CFL-1 showed impaired paclitaxel efficacy,as well as decreased apoptosis rates and pro-survival patterns of apoptosis-specific protein *** accumulation of p-CFL-1 inhibited paclitaxel-induced mitochondrial ***-1 silencing mediated CFL-1 phosphorylation in paclitaxel-resistant SKOV3 ***,the high level of p-CFL-1 and the low level of SSH-1 in EOC tissues were closely related to chemotherapy resistance and poor prognosis in EOC *** The SSH-1/p-CFL-1 signaling pathway mediates paclitaxel resistance by apoptosis inhibition in EOC and is expected to be a potential prognostic predictor.

关键词： Cofilin-1 Slingshot-1 Epithelial ovarian cancer Chemo-resistance Apoptosis

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Urodynamic assessment of bladder storage function after radical hysterectomy for cervical cancer

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Chinese Medical Journal 2020年第19期133卷 2274-2280页

作者： Ting-Ting Cao Hong-Wu Wen Yu-Nong Gao qiu-bo lyu Hui-Xin Liu Sha Wang Shi-Yan Wang Hua-Xin Sun Na Yu Hai-bo Wang Yi Li Zhi-Qi Wang Olivia H.Chang Xiu-Li Sun Jian-Liu Wang Department of Obstetrics and Gynecology Peking University People’s HospitalBeijing 100044China The Key Laboratory of Female Pelvic Floor Disorders Disease Beijing 100044China The Research Center of Female Pelvic Floor Disorders Disease of Peking University Beijing 100044China Department of Obstetrics and Gynecology Peking University First HospitalBeijing 100034China Department of Gynecology Peking University Cancer Hospital&InstituteBeijing 100142China Department of Gynecology Beijing HospitalBeijing 100005China Department of Clinical Epidemiology and Biostatistics Peking University People’s HospitalBeijing 100044China Department of Peking University Medical Information Center Peking UniversityBeijing 100191China Department of Peking University Clinical Research Institute Peking UniversityBeijing 100191China Center for Urogynecology and Pelvic Reconstructive Surgery Cleveland ClinicClevelandOH 44195USA.

Background:After radical hysterectomy for cervical cancer,the most common complication is lower urinary tract ***-operatively,bladder capacity can alter bladder function for a prolonged *** study aimed to identify factors affecting bladder storage ***:A multicenter,retrospective cohort study was *** of patients with stages IA2 to IIB cervical cancer with urodynamic study results were retrospectively collected from nine hospitals between June 2013 and June 2018 according to the inclusion ***,surgical,and oncological data were *** univariate and multivariate logistic regression was used to identify clinical factors associated with bladder storage ***:Two hundred and three patients with cervical cancer had urodynamic testing ***-five(46.8%)patients were diagnosed with stress urinary incontinence(SUI).The incidence of low bladder compliance(LBC)was 23.2%.Twenty-seven(13.3%)patients showed detrusor overactivity(DO).Fifty-seven patients(28.1%)presented with a decreased maximum cystometric capacity(DMCC).The probability of composite bladder storage dysfunction was 68.0%.Multivariate analysis confirmed that laparoscopy represents a protective factor for SUI with an odds ratio of 0.498(P=0.034).Patients who underwent a nerve-sparing procedure were less odds to experience SUI(P=0.014).A significant positive correlation between LBC and DO was observed(P<0.001).A greater length of the resected vagina and chemoradiotherapy were common risk factors for LBC and DO,while radiotherapy exerted a stronger effect than ***,patients who received chemoradiotherapy frequently developed a *** follow-up time was not correlated with bladder storage ***:A nerve-sparing procedure without longer resected vagina is recommended for protecting the bladder storage function.

关键词： Radical hysterectomy Cervical cancer Urodynamic Bladder storage function

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经阴道自然腔道单孔腹腔镜行卵巢囊肿剥除术的可行性和安全性研究

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中国实用妇科与产科杂志 2023年第4期39卷 452-456页

作者：闫瑾博文周丹张烁张波孟庆伟吕秋波脱勋元北京医院妇产科国家老年医学中心中国医学科学院老年医学研究院北京100730 甘肃省妇幼保健院甘肃省中心医院甘肃兰州730050

目的探讨经阴道自然腔道单孔腹腔镜(transvaginal natural orific transluminal endoscopic surgery,vNOTES)行卵巢囊肿剥除术的可行性和安全性。方法该研究是基于“经阴道自然腔道单孔腹腔镜手术(vNOTES)在妇产科良性附件手术中应用的... 详细信息

目的探讨经阴道自然腔道单孔腹腔镜(transvaginal natural orific transluminal endoscopic surgery,vNOTES)行卵巢囊肿剥除术的可行性和安全性。方法该研究是基于“经阴道自然腔道单孔腹腔镜手术(vNOTES)在妇产科良性附件手术中应用的有效性和安全性研究”(临床试验注册号:ChiCTR2100052223)的亚组分析。研究人员于北京医院妇科进行的一项1∶1随机、单中心、非劣效性平行对照试验,非劣效性界值为10%,选取2019年8月至2022年2月在北京医院行卵巢囊肿剥除术的18~50岁符合研究纳入标准的患者,采用分层区组随机方法,根据手术范围分层后,利用随机数字表将纳入的受试者按1∶1随机分入观察组和对照组。观察组通过vNOTES行卵巢囊肿剥除术,对照组通过传统多孔腹腔镜行卵巢囊肿剥除术。主要评价指标为两组通过随机分配的术式完成卵巢囊肿剥除术而未转行其他术式完成手术的比率。次要评价指标为手术时间、术中出血量、术后并发症、术后24 h及1周内视觉模拟(VAS)疼痛评分、术后健康情况和性功能评分等相关数据。结果共有94例受试者纳入研究,观察组和对照组各47例,所有受试者均按照分配的手术方式顺利完成手术,未在术中转行其他手术术式。对手术成功率进行敏感性分析,假设观察组有1例在手术过程中改用其他手术方式,对照组手术全部成功,双侧比例差异的95%置信上限估计为6%,低于10%非劣效性界值。两组受试者术前基线情况、手术时间、术中出血量、健康状况评分及性功能评估差异均无统计学意义(P>0.05)。观察组术后排气时间低于对照组,差异有统计学意义[(18.9±7.7)h vs.(38.2±14.0)h,(P<0.05)];观察组术后7d内每天的VAS疼痛评分均明显低于对照组,差异有统计学意义(P<0.05)。观察组术后3个月、6个月健康情况评分[分别为(129.7±9.1)分和(127.6±10.2)分]较对照组[分别为(119.4±9.8)分和(119.7±10.8)分]高,性功能评估评分[分别为(23.2±8.4)分和(23.2±7.5)分]亦较对照组[分别为(11.1±10.7)分和(10.3±10.7)分]高,差异均有统计学意义(P<0.05)。结论经阴道自然腔道单孔腹腔镜进行卵巢囊肿剥除术是安全可行的,具有疼痛轻、腹部无任何创口、身体恢复快等独特的优势,是一种微创的手术方式,可作为卵巢良性肿瘤治疗的手术方式。

关键词：自然腔道手术经阴道腹腔镜卵巢囊肿

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AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis

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Neural Regeneration Research 2025年第8期20卷 2408-2419页

作者： Ruiqi qiu Mingzhu Yang Xiuxiu Jin Jingyang Liu Weiping Wang Xiaoli Zhang Jinfeng Han bo Lei Henan Eye Hospital Henan Provincial People’s HospitalPeople’s Hospital of Zhengzhou UniversityZhengzhouHenan ProvinceChina Eye Institute Henan Academy of Innovations in Medical ScienceZhengzhouHenan ProvinceChina Branch of National Clinical Research Center for Ocular Disease Henan Provincial People’s HospitalZhengzhouHenan ProvinceChina Academy of Medical Science Zhengzhou UniversityZhengzhouHenan ProvinceChina

Retinitis pigmentosa is a group of inherited diseases that lead to retinal degeneration and photoreceptor cell ***,there is no effective treatment for retinitis pigmentosa caused by PDE6B ***-associated virus(AAV)-mediated gene therapy is a promising strategy for treating retinitis *** aim of this study was to explore the molecular mechanisms by which AAV2-PDE6B rescues retinal *** do this,we injected retinal degeneration 10(rd10)mice subretinally with AAV2-PDE6B and assessed the therapeutic effects on retinal function and structure using dark-and light-adapted electroretinogram,optical coherence tomography,and ***-independent acquisition-mass spectrometry-based proteomic analysis was conducted to investigate protein expression levels and pathway enrichment,and the results from this analysis were verified by real-time polymerase chain reaction and western ***2-PDE6B injection significantly upregulated PDE6βexpression,preserved electroretinogram responses,and preserved outer nuclear layer thickness in rd10 *** expressed proteins between wild-type and rd10 mice were closely related to visual perception,and treating rd10 mice with AAV2-PDE6B restored differentially expressed protein expression to levels similar to those seen in wild-type *** Encyclopedia of Genes and Genome analysis showed that the differentially expressed proteins whose expression was most significantly altered by AAV2-PDE6B injection were enriched in phototransduction ***,the phototransductionrelated proteins Pde6α,Rom1,Rho,Aldh1a1,and Rbp1 exhibited opposite expression patterns in rd10 mice with or without AAV2-PDE6B ***,Bax/Bcl-2,p-ERK/ERK,and p-c-Fos/c-Fos expression levels decreased in rd10 mice following AAV2-PDE6B *** data suggest that AAV2-PDE6B-mediated gene therapy promotes phototransduction and inhibits apoptosis by inhibiting the ERK signaling pathway and upr

关键词： apoptosis AAV2-PDE6B ERK1/2 gene therapy phototransduction proteomics rd10 retinitis pigmentosa

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多种教学实践在妇产科学临床实习中的作用

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西北医学教育 2016年第2期24卷 311-313页

作者：邓文慧吕秋波北京医院妇产科北京100730

妇产科学是一门理论与实践紧密结合的临床学科,操作复杂多样,实践性强,传统教学短期很难达到教学目的。随着近年来临床教学方法的不断积累与改进,我们摸索出一套妇产科学实习的具体教学方法,那就是PBL为主,联合多种方法相互渗透的临床... 详细信息

妇产科学是一门理论与实践紧密结合的临床学科,操作复杂多样,实践性强,传统教学短期很难达到教学目的。随着近年来临床教学方法的不断积累与改进,我们摸索出一套妇产科学实习的具体教学方法,那就是PBL为主,联合多种方法相互渗透的临床教学模式,即在PBL教学基础上采用追踪典型病例、结合病例讨论、结合标本及多媒体教学等。本文对多种方法的实践进行了分析。

关键词：以问题为基础的教学法(PBL) 妇产科学临床实习教学

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人文关怀在胸外科护理管理中的应用

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中国卫生产业 2020年第7期17卷 45-46,49页

作者：罗紫嘉汪洋洋吕秋波吉林省一汽总医院胸外科吉林长春130011

目的探讨人文关怀在胸外科护理管理中的应用。方法抽取2017年5月—2018年5月94例胸外科患者,按照入院顺序分为两组,组名为观察组、对照组,每组47例,观察组给予人文关怀护理管理,对照组实施常规护理管理,对比干预效果。结果观察组服务态... 详细信息

目的探讨人文关怀在胸外科护理管理中的应用。方法抽取2017年5月—2018年5月94例胸外科患者,按照入院顺序分为两组,组名为观察组、对照组,每组47例,观察组给予人文关怀护理管理,对照组实施常规护理管理,对比干预效果。结果观察组服务态度(98.25±0.36)分、护理文书书写(97.25±0.34)分、实践技能(97.25±0.33)分、病区管理(97.25±0.36)分均高于对照组(P<0.05);观察组风险事件发生率2.13%,低于对照组12.77%(P<0.05);观察组护理满意度97.87%高于对照组85.11%(P<0.05)。结论人文关怀在胸外科护理管理中的应用效果显著,可提升护理质量,减低护理风险事件发生率,提高护理满意度。

关键词：人文关怀护理管理胸外科

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2018年FIGO子宫颈癌新分期中ⅡA2期治疗策略的探讨

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中国实用妇科与产科杂志 2021年第11期37卷 1147-1151页

作者：孙立新付佳鑫王莉赵宏伟王丹波林丽红吕秋波郝敏卢逸嘉宾晓农郎景和刘萍陈春林山西省肿瘤医院妇科山西太原030013 南方医科大学南方医院妇产科广东广州510515 河南省肿瘤医院妇科河南郑州450008 中国医科大学肿瘤医院/辽宁省肿瘤医院妇科辽宁沈阳110042 安阳市肿瘤医院妇瘤科河南安阳455000 北京医院妇产科北京100005 山西医科大学附属第二医院妇产科山西太原030001 广州医科大学公共卫生学院广东广州511436 中国医学科学院北京协和医院妇产科北京100730

目的探讨2018年FIGO子宫颈癌新分期中ⅡA2期治疗策略。方法基于中国子宫颈癌临床诊疗项目大数据库,对47家医院于2004—2018年期间收治的1112例FIGO 2018ⅡA2期子宫颈癌病例,在真实世界研究条件下比较开腹广泛性子宫切除手术(ARH组)、新... 详细信息

目的探讨2018年FIGO子宫颈癌新分期中ⅡA2期治疗策略。方法基于中国子宫颈癌临床诊疗项目大数据库,对47家医院于2004—2018年期间收治的1112例FIGO 2018ⅡA2期子宫颈癌病例,在真实世界研究条件下比较开腹广泛性子宫切除手术(ARH组)、新辅助化疗后行手术治疗(NACT组)、根治性放化疗(R-CT组)3种治疗方式的5年总体生存率(OS)及无瘤生存率(DFS)。结果(1)从数据库中共筛选出ARH组471例,NACT组342例,R-CT组299例,中位随访时间45个月。(2)单因素分析结果,ARH组与NACT组5年OS和5年DFS差异无统计学意义(93.6%vs.90.3%,P=0.068;90.0%vs.84.5%,P=0.123);ARH组、NACT组肿瘤学结局均优于R-CT组(ARH vs.R-CT 5年OS:93.6%vs.71.8%,P<0.001;5年DFS:90.0%vs.69.4%,P<0.001;NACT vs.R-CT 5年OS:90.3%vs.71.8%,P<0.001;5年DFS:84.5%vs.69.4%,P<0.001)。(3)多因素分析结果,R-CT组是影响子宫颈癌患者死亡及复发/死亡的独立危险因素。结论对于FIGO 2018ⅡA2期子宫颈癌患者,直接开腹手术或新辅助化疗后手术可能是更好的选择。

关键词：子宫颈肿瘤国际妇产科联盟治疗策略

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A symplectic finite element method based on Galerkin discretization for solving linear systems

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Applied Mathematics and Mechanics(English Edition) 2023年第8期44卷 1305-1316页

作者： Zhiping qiu Zhao WANG bo ZHU Institute of Solid Mechanics School of Aeronautic Science and EngineeringBeihang UniversityBeijing 100083China

We propose a novel symplectic finite element method to solve the structural dynamic responses of linear elastic *** the dynamic responses of continuous medium structures,the traditional numerical algorithm is the dissipative algorithm and cannot maintain long-term energy ***,a symplectic finite element method with energy conservation is constructed in this paper.A linear elastic system can be discretized into multiple elements,and a Hamiltonian system of each element can be *** single element is discretized by the Galerkin method,and then the Hamiltonian system is constructed into the Birkhoffian ***,all the elements are combined to obtain the vibration equation of the continuous system and solved by the symplectic difference *** the numerical experiments of the vibration response of the Bernoulli-Euler beam and composite plate,it is found that the vibration response solution and energy obtained with the algorithm are superior to those of the Runge-Kutta *** results show that the symplectic finite element method can keep energy conservation for a long time and has higher stability in solving the dynamic responses of linear elastic systems.

关键词： Galerkin finite element method linear system structural dynamic response symplectic difference scheme

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A nurse-led long-term pelvic floor muscle training program in the management of female patients with overactive bladder e A study protocol for a randomized controlled trial

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International Journal of Nursing Sciences 2015年第2期2卷 158-166页

作者： Tu-Zhen Xu qiu-Hua Sun Xiao Huang bo-Dong lyu Urology Department Zhejiang Chinese Medical UniversitySecond Affiliated HospitalHangzhouZhejiang 310005China School of Nursing Zhejiang Chinese Medical UniversityHangzhouZhejiang 310053China Urology Department The First Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhouZhejiang 310009China

Background:Previous research has suggested that pelvic floor muscle training(PFMT)offers a therapeutic benefit in patients with overactive ***:Weconducted a single-blind,randomized trial of pelvic floor muscle training(PFMT)as compared with usual *** intervention group(n=54)received a 6-month a nurse-led long-term pelvic floor muscle training program(three sessions a day,15e20 times per session)and the control group(n?53)received usual *** patients received 3-month solifenacin succinate tablets(5 mg e once daily).The treatment outcomes were measured by the Modified Oxford Scale(MOS),Overactive Bladder SymptomScore(OABSS)and the King's Health Questionnaire(KHQ)at baseline,3 months and 6 months ***:Of the 91 randomly assigned patients,46 patients in the PFMT group and 45 patients in the control group completed the *** trial revealed statistically significant differences between groups in pelvic muscle strength at 3 months following the intervention(p0.05).In regards to quality of life,the experimental group showed significant improvements compared to the control group on 6 of 10 domains(p<0.05).At 6 months,there were significant improvements in OABSS scores and quality of life in the experimental group compared to the control group(p<0.05).No adverse events were ***:A nurse-led long-term(6 months)pelvic floor muscle training program may alleviate OAB symptoms effectively and improve the quality of life more than a short term(3 months)pelvic floor muscle training program combined with solifenacin succinate tablets.

关键词： Nurse-led pelvic floor muscle training Symptoms Quality of life Female Overactive bladder

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Towards the Framework of Understanding Autism Spectrum Disorders

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Neuroscience Bulletin 2019年第6期35卷 1110-1112页

作者： Zilong qiu bo Yuan Institute of Neuroscience State Key Laboratory of Neuroscience CAS Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of Sciences

The prevalence of autism spectrum disorders(ASD)has been high worldwide,reaching 1/59 children in the United States as reported by the Centers of Disease Control and *** genetic components play a major role in ASD[1],it is astonishing that the occurrence of ASD would be this high probabily due to genetic *** is worthy to note that autistic phenotypes of ASD patients show great *** severity of autistic symptoms may be correlated with whether genetic mutations affect neural ***,we argue that the prevalence of severe ASD may be much lower than the common ASD usually reported.

关键词： prevalence ed. severity

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