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Villin Family Members Associated with Multiple Stress Responses in Cotton

Phyton-International Journal of Experimental Botany 2021年第6期90卷 1645-1660页

作者： fenni lv Sen Wang Ruiping Tian Peng Wang Kang Liu State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing210095China Institute of Botany Jiangsu Province and Chinese Academy of SciencesNanjing210014China Institute of Food Crops Jiangsu Academy of Agricultural SciencesNanjing210014China

Villin(VLN)is considered to be one of the most important actin-binding proteins,participates in modulating the actin cytoskeleton dynamics,plays essential role in plant development and resisting adverse ***,systematic studies of the VLN gene family have not been reported in cotton(Gossypium).In this study,14 GhVLNs were identified in *** GhVLN genes were distributed in 6 A-subgenome chromosomes and 6 D-subgenome chromosomes of the allotetraploid upland cotton and classified into three phylogenetical groups based on the classification model of *** addition,the 14 GhVLN genes have highly conserved gene structure and motif *** number of introns was ranged from 18 to 22 and the length of protein sequences was varied from 901 to *** gelsolin homology domains,G1–G6,and villin headpiece domain,VHP,were existed in all GhVLNs with the exception of two VLNs(GhVLN6 and GhVLN13)which lacked ***-elements analysis revealed that the promoter regions of GhVLNs contained various light related components and also elements responsible for phytohormones and stresses response,indicating that,when subjected to those adverse environments,cotton plants may activate the response system by targeting VLN genes to survive the *** showed that the GhVLN genes exhibited various expression patterns,some were accumulated in certain tissues,root,petal,stamen or elongating fibers,and some were obviously induced by environmental *** GhVLN3 and GhVLN10 were highly and preferentially expressed in elongating fibers and distinctly upregulated by abiotic(salt,PEG,cold and heat)and biotic(Verticillium dahliae V991)*** study may provide useful information for biological function identification of GhVLN genes and gene resources for creating high-quality and various resistant cotton germplasms.

关键词： Gossypium hirsutum villin proteins cis-elements abiotic and biotic stresses

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Transcriptome analysis of Clematis lanuginosa: Novel features of the molecular events occurring under heat-shock stress

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Ornamental Plant Research 2022年第1期2卷 184-192页

作者： Linfang Li Shuan Wang Qiao Li Linghe Sun Xin Han Lulu Gao Peng Wang fenni lv Rutong Yang Ya Li Institute of Botany Jiangsu Province and Chinese Academy of Sciences(Nanjing Botanical Garden Mem.Sun Yat-Sen)Nanjing 210014China

The weak heat tolerance of Clematis ornamental varieties negatively affects their ornamental qualities in the *** elucidate heat resistance mechanisms,Clematis lanuginosa,which is an important original parent of the Clematis large-flowered group of ornamental varieties,was selected for use in this ***,six libraries,including three biological replicates each of control and heat-shock stress samples,were determined using RNA-sequencing *** total,62,050 unigenes were obtained,and 6,439 unigenes exceeded 1 kb in length.A total of 42,377 unigenes were annotated using six *** the two treatments,2,165 differentially expressed genes were identified,with 1,565 being up-regulated and 600 *** addition,51 heat-shock protein-encoding genes were identified,among which the small heat-shock proteins accounted for 68.63%.In total,two heat-shock factors and 12 ribosomal proteins were significantly up-regulated under heatstress *** differential expressions of ethylene-responsive transcription factor,chalcone synthase,cysteine-rich receptor-like kinase and aspartic protease unigenes in guard cells were induced by *** data obtained will assist the elucidation of the molecular events underlying heat-stress responses in ***.

关键词： shock stress ipt

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Sequence-based ultra-dense genetic and physical maps reveal structural variations of allopolyploid cotton genomes

Sequence-based ultra-dense genetic and physical maps reveal ...

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江苏省遗传学会第九次会员代表大会暨学术研讨会

作者： WANG Sen CHEN Jiedan ZHANG Wenpan HU Yan CHANG Lijing FANG Lei WANG Qiong lv fenni WU Huaitong SI Zhanfeng CHEN Shuqi CAO Caiping ZHU Xiefei ZHOU Baoliang GUO Wangzhen ZHANG Tianzhen State Key Laboratory of Crop Genetics and Germplasm Enhancement Cotton Hybrid R ＆ D Engineering Center （the Ministry of Education） Nanjing Agricultural University

SNPs are the most abundant polymorphism type, and have been explored in many crop genomic studies, including rice and maize. SNP discovery in allotetraploid cotton genomes has lagged behind that of other crops due to their complexity and polyploidy. In this study, genome-wide SNPs are detected systematically using next-generation sequencing and efficient SNP genotyping methods, and used to construct a linkage map and characterize the structural variations in polyploid cotton genomes. We construct anultra-denseinterspecificgeneticmap comprising 4,999,048 SNP loci distributed unevenly in 26 allotetraploid cotton linkage groups and covering 4,042 cM. The map is used to order tetraploid cotton genome scaffolds for accurate assembly of G. hirsutum acc. TM-1. Recombination rates and hotspots are identified across the cotton genome by comparing the assembled draft sequence and the genetic map. Using this map, genome rearrangements and centromeric regions are identified in tetraploid cotton by combining information from the publicly-available G. raimondii genome with fluorescent in situ hybridization analysis. We report the genotype-by-sequencing method used to identify millions of SNPs between G. hirsutum and G. barbadense. We construct and use an ultra-dense SNP map to correct sequence mis-assemblies, merge scaffolds into pseudomolecules corresponding to chromosomes, detect genome rearrangements, and identify centromeric regions in allotetraploid cottons. We find that the centromeric retro-element sequence of tetraploid cotton derived from the D subgenome progenitor might have invaded the A subgenome centromeres after allotetrapolyploid formation. This study serves as a valuable genomic resource for genetic research and breeding of cotton.

关键词： F cotton WGS SNPs genotyping linkage map rearrangement centromeric region

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Wearable battery-free theranostic dental patch for wireless intraoral sensing and drug delivery

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npj Flexible Electronics 2022年第1期6卷 518-528页

作者： Zhenghan Shi Yanli Lu Shuying Shen Yi Xu Chang Shu Yue Wu Jingjiang lv Xin Li Zupeng Yan Zijian An Chaobo Dai Lingkai Su fenni Zhang Qingjun Liu Biosensor National Special Laboratory Key Laboratory for Biomedical Engineering of Education MinistryDepartment of Biomedical EngineeringZhejiang UniversityHangzhou 310027P.R.China Stomatology Hospital School of MedicineZhejiang UniversityHangzhou 310016P.R.China

Dental caries caused by oral microbiome dysbiosis with the elevation of acidogenic bacteria is the most prevalent noncommunicable disease *** prevention and timely fluoride treatment are crucial for caries lesion ***,to address the challenges of in situ sensing and topical drug delivery within the oral cavity,a miniaturized,battery-free,and wearable dental patch system was developed for microenvironment monitoring and controlled fluoride *** the integration of near-field communication,the dental patch realizes wireless energy harvesting and data transmission with mobile terminals like smartphones when attached conformally to the tooth *** acidic microenvironment caused by bacterial metabolism are electrochemically detected,while fluorides can be delivered locally from the electric-responsive drug delivery electrode for on-demand *** flexible electronics armed to the teeth,this intraoral theranostic wearable system provides an inspiring platform for point-of-care monitoring and treatment of dental caries and oral diseases.

关键词： metabolism prevention patch

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基于计算机视觉技术的砀山酥梨果梗识别

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安徽农业大学学报 2015年第4期42卷 642-646页

作者：徐旭艳周平吕冬徐冯黎安徽农业大学工学院合肥230036

针对酥梨果梗计算机视觉检测方法的普适性问题,提出了一种利用像素点分析法识别果梗的算法。将酥梨图像进行二值化、滤波、形态学运算等预处理后,用Sobel算子提取酥梨图像边缘,再由一阶矩特征计算图像形心。选定形心为参考原点,建立图... 详细信息

针对酥梨果梗计算机视觉检测方法的普适性问题,提出了一种利用像素点分析法识别果梗的算法。将酥梨图像进行二值化、滤波、形态学运算等预处理后,用Sobel算子提取酥梨图像边缘,再由一阶矩特征计算图像形心。选定形心为参考原点,建立图像的笛卡尔坐标系;从Y轴负方向开始,逆时针分别按15°和10°形心角对轮廓边缘点进行区域分块;统计分块区域内的像素点特征,并依此特征识别果梗。结果表明,对于无果梗的酥梨,15°和10°均能精确识别出来;有果梗的酥梨,15°较10°的识别率高,且含果梗区域的像素特征较其他区域的有很大突变。总体上果梗识别正确率可达97%,一定程度上可以满足酥梨果梗识别的需要。

关键词：酥梨果梗像素畸变像素点分析

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