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Phylogeny and Geographical Derivation of Genus Hildenbrandia Based on Morphological and Molecular Evidence

Journal of Ocean University of China 2023年第6期22卷 1649-1659页

作者： NAN Fangru ZHAO Huiying LI Juan LIU Xudong LIU Qi lv junping FENG Jia XIE Shulian School of Life Science Shanxi UniversityTaiyuan 030006China

The marine and freshwater Hildenbrandia samples were collected from diverse locations of China and Japan,and mor-phological characterization and molecular phylogenetic analysis were conducted on these samples.Morphological measurements of freshwater specimens were consistent with the results of H.jigongshanensis,whereas cell dimensions of marine specimens were slightly larger than those of widely distributed H.rubra and H.crouanii.Phylogenetic trees based on rbcL,psbA and UPA sequences were consistent.Freshwater specimens collected in this study formed an independent clade with H.jigongshanensis supported by high values.Species attribution was confirmed further by the similar ITS2 secondary structures among the samples of H.jigongshan-ensis.It was found that the intraspecific divergence of rbcL gene in H.jigongshanensis was smaller than other two freshwater species H.rivularis and H.angolensis.Phylogenetic trees showed that marine specimens in this study grouped together with H.rubra samples of North America,and were different with the H.rubra samples of Europe.Combining the comparison results of CBC numbers in ITS2 secondary structure,we propose the marine samples collected in this study to be a new species Hildenbrandia qingdaoensis sp.nov.The separation of the marine and freshwater specimens was supported by the rbcL,UPA and psbA phylogeny as well as ITS2 se-condary structure inference.Biogeographical reconstruction showed the ancestor of freshwater Hildenbrandia might derive from South America and dispersed from North America to Europe and then to Asian countries,which needs to be verified further with more sam-ples and molecular evidences from South America.

关键词： Hildenbrandia morphology molecular phylogeny ITS secondary structure biogeography

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The biological effect of metal ions on the granulation of aerobic granular activated sludge

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Journal of Environmental Sciences 2016年第6期28卷 252-259页

作者： Wen Hao Yaochen Li junping lv Lisha Chen Jianrong Zhu School of Environment Beijing Normal University Beijing 100875 China State Key Laboratory of Water Simulation Beijing 100875 China

As a special biofilm structure,microbial attachment is believed to play an important role in the granulation of aerobic granular activated sludge（AGAS）.This experiment was to investigate the biological effect of Ca^2＋,Mg^2＋,Cu^2＋,Fe^2＋,Zn^2＋,and K＋which are the most common ions present in biological wastewater treatment systems,on the microbial attachment of AGAS and flocculent activated sludge（FAS）,from which AGAS is always derived,in order to provide a new strategy for the rapid cultivation and stability control of AGAS.The result showed that attachment biomass of AGAS was about 300%higher than that of FAS without the addition of metal ions.Different metal ions had different effects on the process of microbial attachment.FAS and AGAS reacted differently to the metal ions as well,and in fact,AGAS was more sensitive to the metal ions.Specifically,Ca^2＋,Mg^2＋,and K＋could increase the microbial attachment ability of both AGAS and FAS under appropriate concentrations,Cu^2＋,Fe^2＋,and Zn^2＋were also beneficial to the microbial attachment of FAS at low concentrations,but Cu^2＋,Fe^2＋,and Zn^2＋greatly inhibited the attachment process of AGAS even at extremely low concentrations.In addition,the acylated homoserine lactone（AHL）-based quorum sensing system,the content of extracellular polymeric substances and the relative hydrophobicity of the sludges were greatly influenced by metal ions.As all these parameters had close relationships with the microbial attachment process,the microbial attachment may be affected by changes of these parameters.

关键词： Microbial attachment Metal ions Aerobic granular activated sludge Flocculent activated sludge

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Performance and role of N-acyl-homoserine lactone(AHL)-based quorum sensing(QS) in aerobic granules

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Journal of Environmental Sciences 2014年第8期26卷 1615-1621页

作者： Yaochen Li junping lv Chen Zhong Wen Hao Yaqin Wang Jianrong Zhu School of Environment Beijing Normal University School of Water Resource and Environment Shijiazhuang University of Economics

The present study investigated the relationship between N-acyl-homoserine lactone（AHL）-based quorum sensing（QS） and the physico-chemical properties of aerobic granules.Stable mature granules were observed in SBR2 and SBR3 with average diameters of 0.96,and1.49 mm,respectively. The sludge densities of aerobic granules in SBR2 and SBR3 were1.0246,and 1.0201 g/mL,respectively,which were higher than that of flocculent sludge in SBR1（1.0065 g/mL）. The results showed that the activity of AHL-based QS in SBR2 and SBR3 amounted to 2.4- and 2.1-fold induction,however,that in SBR1 with flocculent sludge was1.6-fold induction. In addition,the results also showed that the activity of AHL-based QS in the three reactors rose in the feast condition,and then dropped with the consumption of substrate. However,the activity of AHL-based QS in these three reactors recovered again in prolonged starvation. Furthermore,the results showed that the enhancement of AHL-based QS favored the extracellular polymeric substance production of microorganisms in activated sludge. Thus,it could be concluded that aerobic granules showed higher AHL-based QS than flocculent sludge,which resulted from the higher sludge density of aerobic granules than flocculent sludge. AHL-based QS was related to the metabolism energy in the feast condition; however,in prolonged starvation,microorganisms would emit more AHL-like molecules to protect themselves to resist starvation. Moreover,the enhancement of AHL-based QS favored the EPS component productivity of the microorganisms in activated sludge,which contributed to maintain the aerobic granular structure.

关键词： Aerobic granules,N-acyl-homoserine lactone,Quorum sensing,Extracellular polymeric substances

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Fast Blood Oxygenation through Hemocompatible Asymmetric Polymer of Intrinsic Microporosity Membranes

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Research 2024年第1期2023卷 261-270页

作者： Xinxi Huang junping Huang Pengcheng Su Wanbin Li Guangdong Key Laboratory of Environmental Pollution and Health School of EnvironmentJinan UniversityGuangzhou 511443China

Membrane technology has attracted considerable attention for chemical and medical applications,among others.Artificial organs play important roles in medical science.A membrane oxygenator,also known as artificial lung,can replenish O_(2) and remove CO_(2) of blood to maintain the metabolism of patients with cardiopulmonary failure.However,the membrane,a key component,is subjected to inferior gas transport property,leakage propensity,and insufficient hemocompatibility.In this study,we report efficient blood oxygenation by using an asymmetric nanoporous membrane that is fabricated using the classic nonsolvent-induced phase separation method for polymer of intrinsic microporosity-1.The intrinsic superhydrophobic nanopores and asymmetric configuration endow the membrane with water impermeability and gas ultrapermeability,up to 3,500 and 1,100 gas permeation units for CO_(2) and O_(2),respectively.Moreover,the rational hydrophobic–hydrophilic nature,electronegativity,and smoothness of the surface enable the substantially restricted protein adsorption,platelet adhesion and activation,hemolysis,and thrombosis for the membrane.Importantly,during blood oxygenation,the asymmetric nanoporous membrane shows no thrombus formation and plasma leakage and exhibits fast O_(2) and CO_(2) transport processes with exchange rates of 20 to 60 and 100 to 350 ml m^(−2) min^(−1),respectively,which are 2 to 6 times higher than those of conventional membranes.The concepts reported here offer an alternative route to fabricate high-performance membranes and expand the possibilities of nanoporous materials for membrane-based artificial organs.

关键词： porosity asymmetric Oxygen

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Metal complex catalysts broaden bioorthogonal reactions

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Science China Chemistry 2024年第2期67卷 428-449页

作者： Hanjie Zhang Xiaoyu Qin junping Wang Li Ma Tianfeng Chen Department of Chemistry Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and ApplicationsJinan UniversityGuangzhou510632China

Bioorthogonal reactions involving transition metals have diversified applications in imaging,drug development,chemical catalysis and other fields.Transition metals used to catalyze the bioorthogonal reaction mainly include ruthenium,palladium,copper,and gold.However,the great potential for translational applications of bioorthogonal reaction needs to be further expanded and their reaction efficiency should be improved.Therefore,it is an urgent need for the development of this field to find more suitable catalysts to efficiently catalyze existing biological orthogonal reactions and expand the types of biological orthogonal reactions.Thus,this review not only summarizes those transition metal complexes-based catalysts participating in bioorthogonal reaction and some bioorthogonal reactions involving transition metals inside the cells,but also sheds light into the discovery of new transition metal complexes and their future development in applications.

关键词： transition metal complexes bioorthogonal reactions biological applications

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Affinities offreshwater“Chantransia”stage algae(Rhodophyta)from China based on molecular and morphological analyses

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Journal of Oceanology and Limnology 2021年第3期39卷 1063-1076页

作者： Jinfen HAN Fangru NAN Jia FENG junping lv Qi LIU Xudong LIU Shulian XIE School of Life Science Shanxi Key Laboratory for Research and Development of Regional PlantsShanxi UniversityTaiyuan 030006China

Twelve putative"Chantransia"isolates were collected from six locations across Hubei,Shanxi,Guizhou,and Henan Provinces in China.Their morphological characters were evaluated via the Ward’s method of hierarchical cluster analysis in SPSS software to classify phylogenetic relationships among the isolates.The morphological data revealed that isolate HN31(called group"MALL")was distinct from the other specimens being macroscopic with large vegetative cells and monosporangia(>20μm in diameter).The 11 other isolates were grouped into three clusters,corresponding to the species Audouinella tenella,A.hermannii,and A.pygmaea.The morphological"Chantransia"stage of the species,reportedly belonging to the orders Batrachospermales,Thoreales,and the phylogenetically distant genus Audouinella,are very similar,resulting in the di fficulty to distinguish them based just on morphology.Therefore,molecular analysis of rbc L,UPA,and COI-5 P was conducted to infer the phylogenetic position of all isolates in this study.All"A.tenella"and"A.hermannii"sequences belong to order Batrachospermales,while the isolate HN31 was the"Chantransia"of Thorea hispida.The six other specimens varied morphologically,but they were within the circumscription of"A.pygmaea".All three molecular markers show that all"pygmaea"isolates also represent the"Chantransia"of T.hispida.Furthermore,results from this investigation proposed a new species—Sheathia qinyuanensis,corresponding to the isolates QY1 and QY2.

关键词： Batrachospermales COI-5P rbcL Thoreales UPA

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ChatGPT: potential, prospects, and limitations

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Frontiers of Information Technology & Electronic Engineering 2024年第1期25卷 6-11页

作者： Jie ZHOU Pei KE Xipeng QIU Minlie HUANG junping ZHANG School of Computer Science Fudan UniversityShanghai 200433China Department of Computer Science and Technology Tsinghua UniversityBeijing 100084China Shanghai Key Laboratory of Intelligent Information Processing Shanghai 200433China

Recently,OpenAI released Chat Generative Pre-trained Transformer(ChatGPT)(Schulman et al.,2022)(https://***),which has attracted considerable attention from the industry and academia because of its impressive abilities.This is the first time that such a variety of open tasks can be well solved within one large language model.To better understand ChatGPT,we briefly introduce its history,discuss its advantages and disadvantages,and point out several potential applications.Finally,we analyze its impact on the development of trustworthy artificial intelligence,conversational search engine,and artificial general intelligence.

关键词： artificial limitations intelligence

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On the superconvergence of a WG method for the elliptic problem with variable coefficients

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Science China Mathematics 2024年第8期67卷 1899-1910页

作者： junping Wang Xiaoshen Wang Xiu Ye Shangyou Zhang Peng Zhu Division of Mathematical Sciences National Science Foundation Department of Mathematics University of Arkansas at Little Rock Department of Mathematical Sciences University of Delaware College of Data Science Jiaxing University

This article extends a recently developed superconvergence result for weak Galerkin（WG） approximations for modeling partial differential equations from constant coefficients to variable coefficients.This superconvergence features a rate that is two orders higher than the optimal-order error estimates in the usual energy and L2norms. The extension from constant to variable coefficients for the modeling equations is highly non-trivial. The underlying technical analysis is based on a sequence of projections and decompositions. Numerical results confirm the superconvergence theory for second-order elliptic problems with variable coefficients.

关键词： weak Galerkin finite element methods superconvergence second-orderelliptic problems stabilizer-free

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End-to-End Paired Ambisonic-Binaural Audio Rendering

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IEEE/CAA Journal of Automatica Sinica 2024年第2期11卷 502-513页

作者： Yin Zhu Qiuqiang Kong Junjie Shi Shilei Liu Xuzhou Ye Ju-Chiang Wang Hongming Shan junping Zhang Shanghai Key Laboratory of Intelligent Information Processing School of Computer ScienceFudan UniversityShanghai 200433China Beijing ByteDance Technology Co.Ltd. Shanghai 201102China. Chinese University of Hong Kong Hong KongChina IEEE Institute of Science and Technology for Brain-Inspired Intelligence and MOE Frontiers Center for Brain Science Fudan UniversityShanghai 200433 Shanghai Center for Brain Science and Brain-Inspired Technology Shanghai 200031China

Binaural rendering is of great interest to virtual reality and immersive media. Although humans can naturally use their two ears to perceive the spatial information contained in sounds, it is a challenging task for machines to achieve binaural rendering since the description of a sound field often requires multiple channels and even the metadata of the sound sources. In addition, the perceived sound varies from person to person even in the same sound field. Previous methods generally rely on individual-dependent head-related transferred function(HRTF)datasets and optimization algorithms that act on HRTFs. In practical applications, there are two major drawbacks to existing methods. The first is a high personalization cost, as traditional methods achieve personalized needs by measuring HRTFs. The second is insufficient accuracy because the optimization goal of traditional methods is to retain another part of information that is more important in perception at the cost of discarding a part of the information. Therefore, it is desirable to develop novel techniques to achieve personalization and accuracy at a low cost. To this end, we focus on the binaural rendering of ambisonic and propose 1) channel-shared encoder and channel-compared attention integrated into neural networks and 2) a loss function quantifying interaural level differences to deal with spatial information. To verify the proposed method, we collect and release the first paired ambisonic-binaural dataset and introduce three metrics to evaluate the content information and spatial information accuracy of the end-to-end methods. Extensive experimental results on the collected dataset demonstrate the superior performance of the proposed method and the shortcomings of previous methods.

关键词： Ambisonic attention binaural rendering neural network

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MiR-30a-5p alleviates LPS-induced HPMEC injury through regulation of autophagy via Beclin-1

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BIOCELL 2024年第3期48卷 431-441页

作者： RAN PAN JIAYAN MAO YUELIANG ZHENG WEI CHEN junping GUO LIJUN WANG Rainbowfish Rehabilitation and Nursing School Hangzhou Vocational&Technical CollegeHangzhou310018China Cancer Institute of Integrated Traditional Chinese and Western Medicine Key Laboratory of Cancer Prevention and Therapy Combining Traditional Chinese and Western MedicineZhejiang Academy of Traditional Chinese MedicineHangzhou310012China Geriatric Medicine Center Department of EndocrinologyZhejiang Provincial People’s Hospital(Affiliated People’s Hospital)Hangzhou Medical CollegeHangzhou310015China

Background:Sepsis,a type of systemic disease,can impact nearly all organs,tissues and cells.Among them,endothelial cells are amongst theﬁrst to be affected and respond to the insult.In this study,we investigated the protective effects of microRNA-30a-5p(miR-30a-5p)on human pulmonary microvascular endothelial cells(HPMECs)treated with lipolysaccharide(LPS).Methods:An in vitro model of sepsis was established in HPMECs with the use of LPS.Transfecting with different tools(mimetic and inhibitor)to modify miR-30a-5p expression.Cell viability,proliferation and apoptosis were detected by the CCK-8 assay,the EdU kit andﬂuorescence staining,respectively.The autophagy-related protein and mRNA expression,the number of autophagosomes were separately examined through Western blot analysis,qPT-PCR and confocal microscopy.TargetScan and the luciferase reporter assays were used to probe target genes interacting with miR-30a-5p.Results:LPS caused a reduction in the viability and proliferation of HPMECs,as well as an elevation in the number of apoptotic cells.Subsequently,we observed that miR-30a-5p might play a role in preventing LPS-induced inhibition of cell damage and decreasing HPMEC apoptosis,suggesting the potential function of miR-30a-5p in this injury process.Finally,we conﬁrmed that miR-30a-5p exerts its protective effect by regulating cell autophagy,possibly by targeting Beclin-1.Conclusion:Our study provided evidence that autophagy is a crucial aspect in the protective role of miR-30a-5p against LPS-induced HPMEC injury,identifying a promising target for sepsis-related endothelial cell injury.

关键词： Sepsis Endothelial cells MicroRNA Systemic inflammatory response syndrome

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