检索结果-南通市图书馆

Molecular characterization and tissue expression profile of the Dnmts gene family in pig

Journal of Integrative Agriculture 2017年第6期16卷 1367-1374页

作者： luo zong-gang ZHANG Kai CHEN Lei YANG Yuan-xin FU Peng-hui WANG Ke-tian WANG Ling LI Ming-zhou LI Xue-wei ZUO Fu-yuan WANG Jin-yong Chongqing Academy of Animal Science Chongqing 402460 P.R. China Department of Animal Science Southwest University (Rongchang Campus) Chongqing 402460 P.R. China Key Laboratory of Pig Industry Sciences Ministry of Agriculture Chongqing 402460 P.R. China Sichuan Academy of Grassland Sciences Chengdu 610000 P.R.China Institute of Animal Genetics & Breeding College of Animal Science & Technology Sichuan Agricultural University Chengdu 611130 P.R.China

DNA methyltransferases（Dnmts）comprise a family of proteins which involved in the establishment and maintenance of DNA methylation patterns.In pig,the molecular characterization and tissue expression profile of Dnmt gene family are not clear.To solve this problem,reverse transcriptase PCR and rapid amplification of c DNA ends were used to clone the sequences of the porcine Dnmt2 and Dnmt3b genes.Furthermore,the m RNA expression profiles of Dnmt1,Dnmt2,Dnmt3a and Dnmt3b genes from 54 adult tissues and 2 entire fetuses of Rongchang pig were analyzed by quantitative real-time PCR（q RT-PCR）.As a result,the lengths of porcine Dnmt2 and Dnmt3b gene c DNAs were 1 227 and 2 559 bp with cytosine-C5specific DNA methylase domain,respectively.The four Dnmt genes were highly expressed in longissimus dorsi muscle（P〈0.01）.Dnmt1 is highly expressed in heart（P〈0.01）and Dnmt 2 shows its preference in liver and seminal vesicle tissue（P〈0.01）.Dnmt3a and Dnmt3b are highly expressed in the two fetus stages（P〈0.01）.All these results suggested that each gene has its specific expression profile,and deeper study is required to dig more details between the methylation level and Dnmt family m RNA expressions in different tissues.

关键词： pig Dnmt gene family cloning mRNA expression

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Regulating the Electron-Deficient Component in A-DA1D-A Typed Small-Molecule Acceptors for High-Performance Organic Solar Cells

引用

Chinese Journal of Chemistry 2024年第6期42卷 623-630页

作者： Weifei Wei Ruijie Ma Zhanxiang Chen Tongle Xu gang Li Zhenghui luo Shenzhen Key Laboratory of New Information Display and Storage Materials College of Materials Science and EngineeringShenzhen UniversityShenzhenGuangdong518060 China Department of Electronic and Information Engineering Research Institute for Smart Energy (RISE)The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong999077 China

Fine-tuning of the electron-deficient unit in A-DA1D-A typed small-molecule acceptors (SMAs) plays a crucial role in developing efficient SMAs for organic solar cells (OSCs).Here,we developed a SMA based on benzo[4,5]thieno[2,3-b]quinoxaline,designated as QW1,as well as three SMAs based on 1-methylindoline-2,3-dione,identified as QW2,QW3,and QW4.Compared with QW2,QW1 displays slightly blue-shifted absorption spectra and a lower LUMO energy level due to the stronger electron-withdrawing capability of BTQx in contrast to MDO.On the other hand,the introduction of a bromine atom in QW3 and QW4 causes a blue shift in absorption and a reduction in the LUMO energy level compared to QW2.Density functional theory analysis reveals that QW1 exhibits the best molecular planarity,which endows QW1 with larger electron mobility and tighter molecular stacking.Consequently,PM6:QW1 device affords a better efficiency of 15.63% than those of the devices based on QW2 (14.25%),QW3 (13.21%) and QW4 (15.03%).Moreover,the QW4-based device yields the highest open-circuit voltage of 0.933 V,and the PM6:L8-BO:QW4 ternary device realizes a PCE of 19.03%.Overall,our work demonstrates that regulation of electron-deficient central units is an effective strategy to improve the photovoltaic performance of the resulting A-DA1D-A SMAs.

关键词： Organic solar cells Small-molecule acceptor Electron-deficient unit Energy conversion efficiency Renewable resources π-πstacking Green chemistry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A Transient-Pressure-Based Numerical Approach for Interlayer Identification in Sand Reservoirs

引用

Fluid Dynamics & Materials Processing 2024年第3期20卷 641-659页

作者： Hao luo Haibo Deng Honglin Xiao Shaoyang Geng Fu Hou gang luo Yaqi Li Tight Oil and Gas Exploration and Development Project Department PetroChina Southwest Oil and Gas Field CompanyChengdu610051China Energy College Chengdu University of TechnologyChengdu610059China

Almost all sandstone reservoirs contain interlayers. The identification and characterization of these interlayers iscritical for minimizing the uncertainty associated with oilfield development and improving oil and gas recovery.Identifying interlayers outside wells using identification methods based on logging data and machine learning isdifficult and seismic-based identification techniques are expensive. Herein, a numerical model based on seepageand well-testing theories is introduced to identify interlayers using transient pressure data. The proposed modelrelies on the open-source MATLAB Reservoir Simulation Toolbox. The effects of the interlayer thickness, position,and width on the pressure response are thoroughly investigated. A procedure for inverting interlayer parametersin the reservoir using the bottom-hole pressure is also proposed. This method uses only transient pressuredata during well testing and can effectively identify the interlayer distribution near the wellbore at an extremelylow cost. The reliability of the model is verified using effective oilfield examples.

关键词： Sand reservoir interlayer identification transient pressure analysis numerical well test

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Preliminary exploration of animal models of congenital choledochal cysts

引用

World Journal of Gastroenterology 2024年第10期30卷 1420-1430页

作者： Shu-Hao Zhang Yue-Bin Zhang Duo-Te Cai Tao Pan Ken Chen Yi Jin Wen-Juan luo zong-Wei Huang Qing-Jiang Chen Zhi-gang Gao Department of General Surgery Children’s HospitalZhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina

BACKGROUND Various animal models have been used to explore the pathogenesis of choledochal cysts(CCs),but with little convincing results.Current surgical techniques can achieve satisfactory outcomes for treatment of CCs.Consequently,recent studies have focused more on clinical issues rather than basic research.Therefore,we need appropriate animal models to further basic research.AIM To establish an appropriate animal model that may contribute to the investigation of the pathogenesis of CCs.METHODS Eighty-four specific pathogen-free female Sprague-Dawley rats were randomly allocated to a surgical group,sham surgical group,or control group.A rat model of CC was established by partial ligation of the bile duct.The reliability of the model was confirmed by measurements of serum biochemical indices,morpho-logy of common bile ducts of the rats as well as molecular biology experiments in rat and human tissues.RESULTS Dilation classified as mild(diameter,≥1 mm to<3 mm),moderate(≥3 mm to<10 mm),and severe(≥10 mm)was observed in 17,17,and 2 rats in the surgical group,respectively,while no dilation was observed in the control and sham surgical groups.Serum levels of alanine aminotransferase,aspartate aminotrans-ferase,total bilirubin,direct bilirubin,and total bile acids were significantly elevated in the surgical group as compared to the control group 7 d after surgery,while direct bilirubin,total bilirubin,and gamma-glutamyltransferase were further increased 14 d after surgery.Most of the biochemical indices gradually decreased to normal ranges 28 d after surgery.The protein expression trend of signal transducer and activator of transcription 3 in rat model was consistent with the human CC tissues.CONCLUSION The model of partial ligation of the bile duct of juvenile rats could morphologically simulate the cystic or fusiform CC,which may contribute to investigating the pathogenesis of CC.

关键词： Choledochal cyst Animal model Partial ligation Cystic and fusiform dilation Juvenile rats

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Step‑by‑Step Modulation of Crystalline Features and Exciton Kinetics for 19.2%Efficiency Ortho‑Xylene Processed Organic Solar Cells

引用

Nano-Micro Letters 2024年第2期16卷 258-272页

作者： Bosen Zou Weiwei Wu Top Archie Dela Pena Ruijie Ma Yongmin luo Yulong Hai Xiyun Xie Mingjie Li Zhenghui luo Jiaying Wu Chuluo Yang gang Li He Yan Department of Chemistry Department of Chemistry and Hong Kong Branch of Chinese National Engineering Research Center for Tissue Restoration and Reconstruction The Hong Kong University of Science and TechnologyClear Water BayHong KongPeople’s Republic of China Department of Electronic and Information Engineering Research Institute for Smart Energy(RISE)Guangdong‑Hong Kong‑Macao(GHM)Joint Laboratory for Photonic‑Thermal‑Electrical Energy Materials and DevicesThe Hong Kong Polytechnic UniversityHung HomKowloonHong Kong 999077People’s Republic of China The Hong Kong University of Science and Technology Function HubAdvanced Materials ThrustNanshaGuangzhou 511400People’s Republic of China Department of Applied Physics The Hong Kong Polytechnic UniversityHong KongPeople’s Republic of China Shenzhen Key Laboratory of New Information Display and Storage Materials College of Materials Science and EngineeringShenzhen UniversityShenzhen 518060People’s Republic of China

With plenty of popular and effective ternary organic solar cells(OSCs)construction strategies proposed and applied,its power conversion efficiencies(PCEs)have come to a new level of over 19%in single-junction devices.However,previous studies are heavily based in chloroform(CF)leaving behind substantial knowledge deficiencies in understanding the influence of solvent choice when introducing a third component.Herein,we present a case where a newly designed asymmetric small molecular acceptor using fluoro-methoxylated end-group modification strategy,named BTP-BO-3FO with enlarged bandgap,brings different morphological evolution and performance improvement effect on host system PM6:BTP-eC9,processed by CF and ortho-xylene(o-XY).With detailed analyses supported by a series of experiments,the best PCE of 19.24%for green solvent-processed OSCs is found to be a fruit of finely tuned crystalline ordering and general aggregation motif,which furthermore nourishes a favorable charge generation and recombination behavior.Likewise,over 19%PCE can be achieved by replacing spin-coating with blade coating for active layer deposition.This work focuses on understanding the commonly met yet frequently ignored issues when building ternary blends to demonstrate cutting-edge device performance,hence,will be instructive to other ternary OSC works in the future.

关键词： Organic solar cells Ternary design Solvent selection Flouro-methoxylated end group Morphological ordering

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

All-trans retinoic acid alleviates transmissible gastroenteritis virus-induced intestinal inflammation and barrier dysfunction in weaned piglets

引用

Journal of Animal Science and Biotechnology 2024年第3期15卷 1131-1144页

作者： Junning Pu Daiwen Chen gang Tian Jun He Ping Zheng Zhiqing Huang Xiangbing Mao Jie Yu Yuheng luo Junqiu luo Hui Yan Aimin Wu Bing Yu Institute of Animal Nutrition Sichuan Agricultural UniversityChengdu 611130SichuanPeople’s Republic of China Key Laboratory of Animal Disease-Resistance Nutrition Ministry of EducationMinistry of Agriculture and Rural AffairsKey Laboratory of Sichuan Province611130 ChengduSichuanPeople’s Republic of China

Background Transmissible gastroenteritis virus(TGEV)is one of the main pathogens causing severe diarrhea of pig-lets.The pathogenesis of TGEV is closely related to intestinal inflammation.All-trans retinoic acid(ATRA)is the main active metabolite of vitamin A,which has immunomodulatory and anti-inflammatory properties.However,it is unclear whether ATRA can alleviate TGEV-induced intestinal inflammation and barrier dysfunction in piglets.This study aimed to investigate the effects of ATRA on growth performance,diarrhea,intestinal inflammation and intesti-nal barrier integrity of TGEV-challenged piglets.Methods In a 19-d study,32 weaned piglets were randomly divided into 4 treatments:Control group(basal diet),TGEV group(basal diet+TGEV challenge),TGEV+ATRA5 group(basal diet+5 mg/d ATRA+TGEV challenge)and TGEV+ATRA15 group(basal diet+15 mg/d ATRA+TGEV challenge).On d 14,piglets were orally administered TGEV or the sterile medium.Results Feeding piglets with 5 and 15 mg/d ATRA alleviated the growth inhibition and diarrhea induced by TGEV(P<0.05).Feeding piglets with 5 and 15 mg/d ATRA also inhibited the increase of serum diamine oxidase(DAO)activ-ity and the decrease of occludin and claudin-1 protein levels in jejunal mucosa induced by TGEV,and maintained intestinal barrier integrity(P<0.05).Meanwhile,5 mg/d ATRA feeding increased the sucrase activity and the expres-sions of nutrient transporter related genes(GLUT2 and SLC7A1)in jejunal mucosa of TGEV-challenged piglets(P<0.05).Furthermore,5 mg/d ATRA feeding attenuated TGEV-induced intestinal inflammatory response by inhibit-ing the release of interleukin(IL)-1β,IL-8 and tumor necrosis factor-α(TNF-α),and promoting the secretion of IL-10 and secretory immunoglobulin A(sIgA)(P<0.05).Feeding 5 mg/d ATRA also down-regulated the expressions of Toll-like receptors and RIG-I like receptors signaling pathway related genes(TLR3,TLR4,RIG-I,MyD88,TRIF and MAVS)and the phosphorylation level of nuclear factor-κB-p65(NF-κB p65),and up-regulated the inhibitor kappa B alpha(IκBα)p

关键词： All-trans retinoic acid Inflammation Intestinal barrier Piglets Transmissible gastroenteritis virus

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Performance-control-orientated hybrid metal additive manufacturing technologies:state of the art,challenges,and future trends

引用

International Journal of Extreme Manufacturing 2024年第3期6卷 274-328页

作者： Jiming Lv Yuchen Liang Xiang Xu gang Xu Hongmei Zhang Haifei Lu Kaiyu luo Jie Cai Jinzhong Lu School of Mechanical Engineering Jiangsu UniversityZhenjiang 212013People’s Republic of China School of Mechanical Engineering Jiangsu University of Science and TechnologyZhenjiang 212003People’s Republic of China

Metal additive manufacturing(AM)technologies have made significant progress in the basic theoretical field since their invention in the 1970s.However,performance instability during continuous processing,such as thermal history,residual stress accumulation,and columnar grain epitaxial growth,consistently hinders their broad application in standardized industrial production.To overcome these challenges,performance-control-oriented hybrid AM(HAM)technologies have been introduced.These technologies,by leveraging external auxiliary processes,aim to regulate microstructural evolution and mechanical properties during metal AM.This paper provides a systematic and detailed review of performance-control-oriented HAM technology,which is categorized into two main groups:energy field-assisted AM(EFed AM,e.***.ultrasonic,electromagnetic,and heat)technologies and interlayer plastic deformation-assisted AM(IPDed AM,e.***.laser shock peening,rolling,ultrasonic peening,and friction stir process)technologies.This review covers the influence of external energy fields on the melting,flow,and solidification behavior of materials,and the regulatory effects of interlayer plastic deformation on grain refinement,nucleation,and recrystallization.Furthermore,the role of performance-control-oriented HAM technologies in managing residual stress conversion,metallurgical defect closure,mechanical property improvement,and anisotropy regulation is thoroughly reviewed and discussed.The review concludes with an analysis of future development trends in EFed AM and IPDed AM technologies.

关键词： hybrid additive manufacturing in-situ/interlayer plastic deformation auxiliary energy fields microstructure customization mechanical properties enhancement

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Basic Survey Scheduling for the Wide Field Survey Telescope(WFST)

引用

Research in Astronomy and Astrophysics 2024年第1期24卷 59-69页

作者： Yan-Peng Chen Ji-An Jiang Wen-Tao luo Xian-Zhong Zheng Min Fang Chao Yang Yuan-Yu Hong zong-Fei Lü Purple Mountain Observatory Chinese Academy of SciencesNanjing 210023China School of Astronomy and Space Science University of Science and Technology of ChinaHefei 230026China Department of Astronomy University of Science and Technology of ChinaHefei 230026China National Astronomical Observatory of Japan National Institutes of Natural Sciences2-21-1 OsawaMitakaTokyo 181-8588Japan Institute of Deep Space Sciences Deep Space Exploration LaboratoryHefei 230026China

Aiming at improving the survey efficiency of the Wide Field Survey Telescope, we have developed a basic scheduling strategy that takes into account the telescope characteristics, observing conditions, and weather conditions at the Lenghu site. The sky area is divided into rectangular regions, referred to as “tiles,” with a size of2°. 577 × 2°. 634 slightly smaller than the focal area of the mosaic CCDs. These tiles are continuously filled in annulars parallel to the equator. The brightness of the sky background, which varies with the moon phase and distance from the moon, plays a significant role in determining the accessible survey fields. Approximately 50connected tiles are grouped into one block for observation. To optimize the survey schedule, we perform simulations by taking into account the length of exposures, data readout, telescope slewing, and all relevant observing conditions. We utilize the Greedy Algorithm for scheduling optimization. Additionally, we propose a dedicated dithering pattern to cover the gaps between CCDs and the four corners of the mosaic CCD array, which are located outside of the 3° field of view. This dithering pattern helps to achieve relatively uniform exposure maps for the final survey outputs.

关键词： telescopes surveys Moon

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Interface engineering of Co_(9)S_(8)/SnS heterostructure as a high-performance anode for lithium/sodium-ion batteries

引用

Rare Metals 2024年第2期43卷 612-623页

作者： Jia luo Shi-Li Xiang Dan-Yang Han An Liu João Cunha gang-Yong Li Zhao-Hui Hou Hong Yin School of Chemistry and Chemical Engineering Key Laboratory of Hunan Province for Advanced Carbon-based Functional MaterialsHunan Institute of Science and TechnologyYueyang414006China Hubei Jiufengshan Laboratory Wuhan430206China International Iberian Nanotechnology Laboratory(INL) Av.Mestre Jose Veiga4715-330BragaPortugal

Transition metal sulfide(TMS)anodes exhibit the characteristics of phase stability and high capacity for lithium/sodium-ion batteries(LIBs/SIBs).However,the TMS anodes often suffer from poor electronic conductivity,low ionic diffusion and large volume expansion during Li/Na-ion intercalation significantly impairing the Li/Na-storage performance.Herein,a long chain heterostructure composed of the Co_(9)S_(8) and SnS are first reported,which can generate rich phase interfaces,and small crystal domains.The unique structure can facilitate the properties of reactivity,conductivity and ionic diffusion.In addition,the heterostructure surface is modified by the N-doped carbon(N-DC@(CoSn)S),successfully improving the structural stability.The synergistic effects of Co_(9)S_(8)/SnS heterostructure and coated carbon layer effectively increase the capacity and cycling stability.The N-DC@(CoSn)S anode delivers enhanced high specific capacities of 820.6 mAh·g^(−1) at 1.0 A·g^(–1) after 500 cycles for LIBs and 339.2 mAh·g^(–1)at 0.5 A·g^(–1) after 1000 cycles for SIBs,respectively.This work is expected to provide a material design idea for preparing LIBs/SIBs with high capacity and long cycling life.

关键词： Interface engineering Long chain heterostructure High capacity Cycling stability Li Na-ion batteries

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Solid-state atomic hydrogen as a broad-spectrum RONS scavenger for accelerated diabetic wound healing

引用

National Science Review 2024年第2期11卷 196-206页

作者： Man luo Qin Wang gang Zhao Wei Jiang Cici Zeng Qingao Zhang Ruyu Yang Wang Dong Yunxi Zhao Guozhen Zhang Jun Jiang Yucai Wang Qing Zhu Key Laboratory of Precision and Intelligent Chemistry School of Chemistry and Materials Science University of Science and Technology of China Department of Radiology The First Affiliated Hospital of University of Science and Technology of China Division of Life Sciences and Medicine University of Science and Technology of China Shenzhen Senior High School Institute of Intelligent Innovation Henan Academy of Sciences

Hydrogen therapy shows great promise as a versatile treatment method for diseases associated with the overexpression of reactive oxygen and nitrogen species（RONS）. However, developing an advanced hydrogen therapy platform that integrates controllable hydrogen release, efficient RONS elimination, and biodegradability remains a giant technical challenge. In this study, we demonstrate for the first time that the tungsten bronze phase H0.53WO3（HWO） is an exceptionally ideal hydrogen carrier, with salient features including temperature-dependent highly-reductive atomic hydrogen release and broad-spectrum RONS scavenging capability distinct from that of molecular hydrogen. Moreover, its unique p H-responsive biodegradability ensures post-therapeutic clearance at pathological sites. Treatment with HWO of diabetic wounds in an animal model indicates that the solid-state atomic H promotes vascular formation by activating M2-type macrophage polarization and anti-inflammatory cytokine production, resulting in acceleration of chronic wound healing. Our findings significantly expand the basic categories of hydrogen therapeutic materials and pave the way for investigating more physical forms of hydrogen species as efficient RONS scavengers for clinical disease treatment.

关键词： atomic hydrogen RONS scavenger inflammatory regulation diabetic wound healing hydrogen therapy

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：