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基于国内外大豆品种比较的中国大豆品种改良路径探索

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大豆科学 2024年第4期43卷 395-401页

作者：郭勇姜思琦于莉莉徐江源洪慧龙邱丽娟中国农业科学院作物科学研究所/作物基因资源与育种全国重点实验室/农作物基因资源与遗传改良国家重大科学工程/农业农村部北京大豆生物学重点实验室北京100081

我国的大豆生产落后于世界大豆主产国,从品种角度看,存在着单产水平低、油分含量低等问题。本文对主产国大豆品种研发应用情况以及美国大豆品种发展趋势进行分析,明确了美国在大豆种质的资源研究、育种技术研发、品种特性鉴定以及环境... 详细信息

我国的大豆生产落后于世界大豆主产国,从品种角度看,存在着单产水平低、油分含量低等问题。本文对主产国大豆品种研发应用情况以及美国大豆品种发展趋势进行分析,明确了美国在大豆种质的资源研究、育种技术研发、品种特性鉴定以及环境因素研究等方面对于大豆品种研发的促进作用。通过对中美大豆品种特性的全面比较分析,明确我国大豆品种在单产、含油量、种植密度等方面的差距,提出了快速提升我国大豆品种产量和品质的路径,即通过对高油大豆资源进行表型精准鉴定,研发全基因组选择、转基因等高效育种技术,聚合产量和其他优异性状,创制优异大豆种质,设计培育高油高产突破性品种,以期为中国大豆品种改良研究提供参考。

关键词：大豆品种改良高油高产路径

Ideal type 1 is caused by a point mutation in the a-tubulin gene that affects microtubule arrangement in soybean

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The crop Journal 2023年第3期11卷 713-722页

作者： Bohong Su Yong Guo Zhongfeng Li Huawei Gao Zhongyan Wei lijuan qiu College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina national key facility for crop gene resources and genetic improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China College of Agronomy Henan Agricultural UniversityZhengzhou 450002HenanChina State key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Key Laboratory of Biotechnology in Plant Protection of MARA of China and Zhejiang ProvinceInstitute of Plant VirologyNingbo UniversityNingbo 315211ZhejiangChina

Plant architecture is a target of crop *** soybean mutant ideal type 1(it1)displays a pleiotropic phenotype characterized by compact plant architecture,reduced plant height,shortened petioles,wrinkled leaves,and indented *** analysis revealed that the pleiotropic phenotype was controlled by an incomplete dominant *** characterized the cellular phenotypes of it1 and positionally cloned the it1 *** morphogenetic analysis of the it1 mutant revealed an excess of xylem cells and expanded phloem,and polygonal pavement *** cloning showed that the phenotype was caused by a G-to-A mutation in the second exon of the a-tubulin gene(Glyma.05G157300).The mutation altered microtubule arrangement in pavement cells,changing their *** of Gmit1 resulted in an it1-like phenotype and polygonal pavement cells and microtubules of overexpressors were parallel or slightly *** suppressor mutants able to suppress the phenotype of it1 were obtained by EMS mutagenesis in the it1 *** these mutants carried an additional mutation in the it1 *** results suggest that the pleiotropic phenotype of it1 is caused by the mutation in the atubulin gene.

关键词： Soybean Plant architecture a-Tubulin Microtubules Intragenic mutations

Molecular characterization of GmNHX2,a Na^+/H^+ antiporter gene homolog from soybean,and its heterologous expression to improve salt tolerance in Arabidopsis

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chinese science Bulletin 2009年第19期54卷 3536-3545页

作者： ZHOU GuoAn GUAN RongXia LI YingHui CHANG RuZhen qiu lijuan national key facility for crop gene resources and genetic improvement (NFCRI)/key Lab of Germplasm & Biotechnology (MOA) Institute of Crop Science Chinese Academy of Agricultural Sciences Beijing 100081 China

Na+/H+ antiporters have been well documented to enhance plant salt tolerance by regulating cellular ion homeostasis. Here, a putative Na+/H+ antiporter gene homolog GmNHX2 from soybean was cloned and predicted to encode a protein of 534 amino acids with 10 putative transmembrane domains. GmNHX2 was expressed in all soybean plant tissues but enriched in roots and its expression was induced by NaCl and polyethylene glycol (PEG) treatments. GmNHX2 exhibits greater sequence similarity with LeNHX2 and AtNHX6 than that of AtNHX1 and AtSOS1. Although phylogenetic analysis clustered GmNHX2 with organellar (tonoplast and vesicles) antiporters, the GmNHX2-EGFP (enhanced green fluorescent protein) fusion protein was possibly localized in the plasma membrane or organelle membrane of transgenic plant cells. Furthermore, transgenic Arabidopsis plants expressing GmNHX2 were more tolerant to high NaCl concentrations during germination and seedling stages when compared with wild-type plants. These results suggest that GmNHX2 is a membrane Na+/H+ antiporter and may function to regulate ion homeostasis under salt stress.

关键词：植物耐盐基因工程盐胁迫离子分布植物组织 GmNHX2

QTL mapping of qSCN3-1 for resistance to soybean cyst nematode in soybean line Zhongpin 03-5373

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The crop Journal 2021年第2期9卷 351-359页

作者： Lei Yang Yu Tian Yulin Liu Jochen C.Reif Yinghui Li lijuan qiu The national key facility for crop gene resources and genetic improvement(NFCRI)/key Lab of Germplasm Utilization(MOA) Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China College of Forestry Northwest A&F UniversityYangling 712100ShaanxiChina Department of Breeding Research Leibniz Institute of Plant Genetics and Crop Plant Research(IPK)GaterslebenGermany

Soybean cyst nematode(SCN,Heterodera glycines Ichinohe)is one of the most economically destructive *** soybean line Zhongpin03-5373(ZP),which combines resistance genes from several donors,is highly resistant to SCN race 3(SCN3).In our previous study,two QTL(rhg1 and GmSNAP11)were identified in a population of recombinant inbred lines derived from a cross between ZP and the susceptible parent Zhonghuang *** two QTL explained around one-third of the resistance,suggesting the presence of further QTL contributing to SCN *** the present study,we used an improved version of the geneticmap comprising the previously applied 1062 molecular markers and 47 newly developed InDel(insertion-deletion)*** improved map revealed a novel locus contributing to SCN3 resistance:qSCN3-1,flanked by InDelmarker InDel1-7 and SNPmarker Map-0047,explained 4.55%of the phenotypic variance for resistance to SCN3 and was not involved in digenic epistatic interaction with rhg1 and *** of Map-0047_CAPS(a CAPS marker developed for Map-0047)and InDel1-7 were significantly associated with SCN3 resistance in a panel of 209 resistant and susceptible *** further allele-combination analysis for three functional markers representing three cloned resistance genes(rhg1,Rhg4,andGmSNAP11)and twomarkers flanking qSCN3-1,we found that adding the resistance allele of qSCN3-1 greatly increased soybean resistance to SCN,even in diverse genetic *** qSCN3-1 locus will be useful for marker-assisted polygene pyramid breeding and should be targeted for the future identification of candidate genes.

关键词： Allelic combination InDel Pyramid Soybean cyst nematode

Development and utilization of a new chemically-induced soybean library with a high mutation density

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Journal of Integrative Plant Biology 2017年第1期59卷 60-74页

作者： Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu jinhui Lei Ying Liu Rongxia Guan Yong Guo longguo jin lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang lijuan qiu national key facility for gene resources and genetic improvement/key Laboratory of crop Germplasm Utilization Ministry of AgricultureInstitute of Crop ScienceChinese Academy of Agricultural Science Soybean Research institute Heilongjiang Academy of Agricultural Sciences College of Agriculture Yangtze University

Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate（EMS）-induced soybean（Glycine max） population,consisting of 21,600 independent M_2 lines, was *** 1,000 M_（4（5））families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll（Chl） content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene（ChlI1a） results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean.

关键词： soybean mutant yellow chemically golden phenotype genomics mutation Glycine chloroplast

SPP-extractor:Automatic phenotype extraction for densely grown soybean plants

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作物学报:英文版 2023年第5期11卷 1569-1578页

作者： Wan Zhou Yijie Chen Weihao Li Cong Zhang Yajun Xiong Wei Zhan Lan Huang Jun Wang lijuan qiu College of Computer science Yangtze UniversityJingzhou 434023HubeiChina College of Agriculture Yangtze UniversityJingzhou 434025HubeiChina national key facility for gene resources and genetic improvement/institute of crop sciences Chinese Academy of Agricultural SciencesBeijing 100081China MARA key Laboratory of Sustainable crop Production in the Middle Reaches of the Yangtze River(Co-construction by Ministry and Province) College of AgricultureYangtze UniversityJingzhou 434025HubeiChina

Automatic collecting of phenotypic information from plants has become a trend in breeding and smart *** mature soybean plants at the harvesting stage,which are dense and overlapping,we have proposed the SPP-extractor(soybean plant phenotype extractor)algorithm to acquire phenotypic ***,to address the mutual occultation of pods,we augmented the standard YOLOv5s model for target detection with an additional attention *** resulting model could accurately identify pods and stems and could count the entire pod set of a plant in a single ***,considering that mature branches are usually bent and covered with pods,we designed a branch recognition and measurement module combining image processing,target detection,semantic segmentation,and heuristic *** results on real plants showed that SPP-extractor achieved respective R^(2) scores of 0.93–0.99 for four phenotypic traits,based on regression on manual measurements.

关键词： Soybean phenotype Branch length Computer vision A*algorithm Phenotype acquisition

Gm Ms1 encodes a kinesin-like protein essential for male fertility in soybean(Glycine max L.)

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Journal of Integrative Plant Biology 2021年第6期63卷 1054-1064页

作者： Muhammad Nadeem Andong Chen Huilong Hong Dongdong Li Jiajia Li Duo Zhao Wei Wang Xiaobo Wang lijuan qiu School of Agronomy Anhui Agricultural UniversityHefei 230036China The national key facility for crop gene resources and genetic improvement(NFCRI)/key Laboratory of crop gene Resource and Germplasm Enhancement(MOA) Institute of Crop SciencesThe Chinese Academy of Agricultural SciencesBeijing 100081China

The application of heterosis is a promising approach for greatly increasing yield in soybean(Glycine max L.). Nuclear male sterility is essential for hybrid seed production and the utilization of heterosis. Here we report the cloning of the gene underlying the soybean male-sterile mutant ms-1,which has been widely used for recurrent selection in soybean breeding programs. We initially delimited the ms1 locus to a 16.15 kb region on chromosome13, based on SLAF_BSA sequencing followed by genotyping of an F_(2) population segregating for the locus. Compared with the same region in fertile plants, the mutant region lacks a sequence of approximately 38.7 kb containing five protein-coding genes, including an ortholog of the kinesin-like protein gene NACK2, named Gm Ms1. The Gm Ms1 knockout plants generated via CRISPR/Casmediated gene editing displayed a complete malesterile phenotype. Metabolic profiling showed that fertile anthers accumulated starch and sucrose normally, whereas sterile anthers had higher anthocyanin levels and lower flavonoid levels and lower antioxidant enzyme activities. These results provide insights into the molecular mechanisms governing male sterility and demonstrate that Gm Ms1 could be used to create male-sterile lines through targeted mutagenesis. These findings pave the way for designing seed production technology and an intelligent male-sterile line system to utilize heterosis in soybean.

关键词： metabolome sequencing nuclear male sterility soybean third generation sequencing transcriptome sequencing

Involvement of sulfur assimilation in the low β subunit content of soybean seed storage protein revealed by comparative transcriptome analysis

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The crop Journal 2019年第4期7卷 504-515页

作者： Xi Zhang Ruixin Xu Wei Hu Wan Wang Dezhi Han Fan Zhang Yongzhe Gu Yong Guo Jun Wang lijuan qiu College of Agriculture Yangtze University Jingzhou 434025 Hubei China national key facility for gene resources and genetic improvement Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China Soybean Research Center Heihe Institute Heilongjiang Academy of Agricultural Sciences Heihe 100142 Helongjiang China

The β subunit of soybean [Glycine max (L.) Merr.] seed storage protein is of great significance in sulfur-containing amino acid balance and soybean processing properties. The objective of this study was to elucidate the relationship between the β subunit and sulfur-containing amino acid composition, and the potential regulatory mechanism. The β subunit was independently accumulated in comparison with other major subunits (α/α′, acidic, basic, and A3) during seed filling, and a low level of β subunit content (BSC) was formed during the accumulation process. In low-BSC mature seeds, crude protein, oil content, and fatty acid composition were not changed, but sulfur-containing amino acids (Cys + Met) in the low- BSC seeds increased significantly (by 31.5%), suggesting that an internal regulatory mechanism within seed might be responsible for the rebalance of seed protein composition and that sulfur assimilation might be deeply involved in β subunit accumulation. Transcriptomic analysis revealed that genes involved in anabolism of cysteine, methionine, and glutathione were up-regulated but those involved in the catabolism of these compounds were down-regulated, suggesting a relationship between the elevation of methionine and glutathione and low BSC. Our study sheds light on seed composition in low BSC lines and on the potential molecular regulatory mechanism of β subunit accumulation, broadening our understanding of soybean seed protein synthesis and its regulation.

关键词： Soybean β subunit Transcriptome analysis Sulfur-containing amino acids Sulfur assimilation

Identification of genes for drought resistance and prediction of gene candidates in soybean seedlings based on linkage and association mapping

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The crop Journal 2022年第3期10卷 830-839页

作者： Yanjun Zhang Zhangxiong Liu Xingrong Wang Yue Li Yongsheng Li Zuowang Gou Xingzhen Zhao Huilong Hong Honglei Ren Xusheng Qi lijuan qiu institute of crop Gansu Academy of Agricultural SciencesLanzhou 730070GansuChina national key facility for gene resources and genetic improvement/key Laboratory of crop Germplasm Utilization Ministry of Agriculture/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Maize Research institute Heilongjiang Academy of Agricultural SciencesHarbin 150086HeilongjiangChina

Drought is one of the primary abiotic stress factors affecting the yield,growth,and development of *** extreme cases,drought can reduce yield by more than 50%.The seedling stage is an important determinant of soybean growth:the number and vigor of seedlings will affect growth and yield at ***,it is important to study the drought resistance of soybean *** this study,a recombinant inbred line(RIL)population comprising 234 F_(6:10)lines(derived from Zhonghuang35×jindou 21)and a panel of 259 soybean accessions was subjected to drought conditions to identify the effects on phenotypic traits under these *** a genetic map constructed by single nucleotide polymorphism(SNPs)markers,18 quantitative trait loci(QTL)on 7 soybean chromosomes were identified in two *** included 9 QTL clusters identified in the RIL ***-three QTL were identified in 19 soybean chromosomes by genome-wide association analysis(GWAS)in the panel of accessions,including 69 significant SNPs(-log_(10)(P)≥3.97).A combination of the two populations revealed that two SNPs(-log_(10)(P)≥3.0)fell within two of the QTL(qPH7-4 and qPH7-6)confidence *** not only re-located several previously reported drought-resistance genes in soybean and other crops but also identified several non-synonymous stress-related mutation site differences between the two parents,involving Glyma.07 g093000,Glyma.07 g093200,Glyma.07 g094100 and Glyma.07 *** previously unreported new gene related to drought stress,Glyma.07 g094200,was found by regional association *** significant SNP CHR7-17619(G/T)was within an exon of the Glyma.07 g094200 *** the RIL population,the DSP value of the"T"allele of CHR7-17619 was significantly(Pgenes in soybeans at the seedling stage.

关键词： Candidate genes Drought tolerance Glycine max QTL SNP