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Coxsackievirus B3 Infection Triggers Autophagy through 3 Pathways of Endoplasmic Reticulum Stress

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Biomedical and Environmental Sciences 2018年第12期31卷 867-875页

作者： luo xiao nuan YAO Hai Lan SONG Juan SONG Qin Qin SHI Bing Tian XIA Dong HAN Jun State Key Laboratory of Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Molecular Immunology Laboratory Capital Institute of Pediatrics

Objective Autophagy is a highly conserved intracellular degradation pathway. Many picornaviruses induce autophagy to benefit viral replication, but an understanding of how autophagy occurs remains incomplete. In this study, we explored whether coxsackievirus B3(CVB3) infection induced autophagy through endoplasmic reticulum(ER) stress. Methods In CVB3-infected HeLa cells, the specific molecules of ER stress and autophagy were detected using Western blotting, reverse transcription polymerase chain reaction(RT-PCR), and confocal microscopy. Then PKR-like ER protein kinase(PERK) inhibitor, inositol-requiring protein-1(IRE1) inhibitor, or activating transcription factor-6(ATF6) inhibitor worked on CVB3-infected cells, their effect on autophagy was assessed by Western blotting for detecting microtubule-associated protein light chain 3(LC3). Results CVB3 infection induced ER stress, and ER stress sensors PERK/eIF2α, IRE1/XBP1, and ATF6 were activated. CVB3 infection increased the accumulation of green fluorescent protein(GFP)-LC3 punctuation and induced the conversion from LC3-Ⅰ to phosphatidylethanolamine-conjugated LC3-1(LC3-Ⅱ). CVB3 infection still decreased the expression of mammalian target of rapamycin(mTOR) and p-mTOR. Inhibition of PERK, IRE1, or ATF6 significantly decreased the ratio of LC3-Ⅱ to LC3-Ⅰ in CVB3-infected HeLa cells. Conclusion CVB3 infection induced autophagy through ER stress in HeL a cells, and PERK, IRE1, and ATF6 a pathways participated in the regulation of autophagy. Our data suggested that ER stress may inhibit mTOR signaling pathway to induce autophagy during CVB3 infection.

关键词： Coxsackievirus B3(CVB3) Autophagy Endoplasmic reticulum(ER) stress Unfolded protein response(UPR)

Exploration of IRES Elements within the ORF of the Coxsackievirus B3 Genome

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Biomedical and Environmental Sciences 2022年第4期35卷 322-333页

作者： SONG Qin Qin luo xiao nuan SHI Bing Tian LIU Mi SONG Juan XIA Dong XIA Zhi Qiang WANG Wen Jun YAO Hai Lan HAN Jun State Key Laboratory of Infectious Disease Prevention and Control National Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Molecular Immunology Laboratory Capital Institute of PediatricsBeijing 100020China

Objective This study aimed to identify internal ribosome entry sites(IRESs)in the open reading frame(ORF)of the Coxsackievirus B3(CVB3)*** The sequences of P1,P2,or P3 of the CVB3 genome or the truncated sequences from each antithymocyte globulin(ATG)to the end of the P1,P2,or P3 gene were inserted into the *** transfection,possible IRES-dependent green fluorescent protein(GFP)-fused proteins were detected by anti-GFP western *** sequences of possible IRESs were inserted into specific Fluc/Rluc bicistronic vectors,in which the potential IRESs were determined according to the Fluc/Rluc activity *** of Fluc and Rluc mRNA of the bicistronic vector was detected by *** After transfection of full length or truncated sequences of the P1,P2,or P3 plasmids,six GFPfused protein bands in P1,six bands in P2 and nine bands in P3 were detected through western *** IRESs in VP2(1461–1646 nt)and VP1(2784–2983 nt)of P1;one IRES in 2C(4119–4564 nt)of P2;and two IRESs in 3C(5634–5834 nt)and 3D(6870–7087 nt)of P3 were identified according to Fluc/Rluc activity *** cryptic promoter was also excluded by *** Five IRESs are present in the CVB3 coding region.

关键词： CVB3 ORF P1 P2 P3 IRESs

Viral Contamination Source in Clinical Microbiology Laboratory

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Biomedical and Environmental Sciences 2016年第8期29卷 609-611页

作者： WANG Xin Ling SONG Juan SONG Qin Qin YU Jie luo xiao nuan WU Gui Zhen HAN Jun State Key Laboratory for Infectious Disease Prevention and Control Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and Prevention National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

To understand the potential causes of laboratory-acquired infections and to provide possible solutions that would protect laboratory personnel, samples from a viral laboratory were screened to determine the main sources of contamination with six subtypes of Rhinovirus. Rhinovirus contamination was found in the gloves, cuffs of protective wear, inner surface of biological safety cabinet （BSC） windows, and trash handles. Remarkably, high contamination was found on the inner walls of the centrifuge and the inner surface of centrifuge tube casing in the rotor. Spilling infectious medium on the surface of centrifuge tubes was found to contribute to contamination of centrifuge surfaces. Exposure to sodium hypochlorite containing no less than 0.2 g/L available chlorine decontaminated the surface of the centrifuRe tubes from Rhinovirus after 2 min.

关键词： Viral Contamination Source in Clinical Microbiology Laboratory BSC HRV

蓝舌病病毒VP2蛋白展示口蹄疫病毒中和表位的研究

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中国预防兽医学报 2014年第9期36卷 731-734页

作者：罗小暖王芳崔玉东于力黑龙江八一农垦大学生命科学技术学院黑龙江大庆163319 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/牛羊传染病研究创新团队黑龙江哈尔滨150001

为在蓝舌病病毒(BTV)VP2蛋白中鉴定展示表达外源B细胞表位的位点,本研究通过同源建模预测VP2蛋白的结构,采用SOE-PCR方法,将O型口蹄疫病毒编码保守的中和表位(8E8)序列分别插入BTV-8 VP2基因编码的两个不同loop区序列中,并分别构建了两... 详细信息

为在蓝舌病病毒(BTV)VP2蛋白中鉴定展示表达外源B细胞表位的位点,本研究通过同源建模预测VP2蛋白的结构,采用SOE-PCR方法,将O型口蹄疫病毒编码保守的中和表位(8E8)序列分别插入BTV-8 VP2基因编码的两个不同loop区序列中,并分别构建了两种嵌合VP2基因。将嵌合表位编码序列的VP2基因克隆于杆状病毒转移载体pFastBac HTb中,转化感受态细胞DH10Bac,筛选阳性重组杆粒rBacmid-VP2-8E8,转染昆虫细胞Sf9,获得重组杆状病毒rBac-VP2-8E8。经western blot和间接免疫荧光验证,嵌合8E8表位的VP2蛋白在Sf9细胞中正确表达,并且8E8表位能够正确展示在VP2蛋白表面;VP2蛋白三聚体分析表明,嵌合8E8表位的VP2蛋白仍能形成三聚体。以上结果表明,BTV VP2蛋白至少存在两个插入位点,能够容纳12个氨基酸短肽的插入。该结果为BTV病毒样颗粒展示8E8表位的研究提供实验依据。

关键词：蓝舌病病毒 VP2蛋白 O型口蹄疫病毒8E8表位杆状病毒表达

BIBR1532 inhibits proliferation and metastasis of esophageal squamous cancer cells by inducing telomere dysregulation

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 214-225页

作者： Qin Wang Qing-Rong Li Lei Xu Zi-Chun Yuan xiao Liu Mao-Ju Tang Man luo xiao-Wu Zhong Qiang Ma xiao-Lan Guo Department of Clinical Laboratory Affiliated Hospital of North Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina School of Laboratory Medicine&Translational Medicine Research Center North Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina

BACKGROUND Esophageal squamous cell carcinoma(ESCC)is a malignant tumor with high morbidity and mortality,and easy to develop resistance to chemotherapeutic *** are DNA-protein complexes located at the termini of chro-mosomes in eukaryotic cells,which are unreplaceable in maintaining the stability and integrity of ***,an RNA-dependent DNA polymerase,play vital role in telomere length maintain,targeting telomerase is a promising therapeutic strategy for ***150 and KYSE410 cells were cultured and exposed to various concentrations of *** viability was assessed at 48 hours and 72 hours to determine the IC50 *** effects of BIBR1532 on ESCC cell proliferation,migration,and cellular senescence were evaluated using the cell counting kit-8 assay,plate colony formation assay,scratch assay,transwell assay,andβ-galactosidase staining,*** blotting was performed to detect the expression of RESULTS The IC50 values for KYSE150 and KYSE410 cells after 48 hours of BIBR1532 exposure were 48.53μM and 39.59μM,*** values decreased to 37.22μM and 22.71μM,respectively,following a longer exposure of 72 ***1532 exhibited dose-dependent effects on KYSE150 and KYSE410 cells,including decreased hTERT expression,inhibition of proliferation and metastasis,and induction of cellular ***,BIBR1532 upregulated the expression of the DDR protein,γ-H2AX,and activated the ataxia telangiectasia and Rad3-related protein(ATR)/check point kinase 1(CHK-1)and ataxia-telangiectasia mutated gene(ATM)/CHK2 ***1532 downregulated the expression of telomere-binding proteins,including telomeric-repeat binding factor 1(TRF1),TRF2,protection of telomeres 1,and TIN2-interacting protein *** a nude mouse xenograft model,BIBR1532 significantly suppressed tumor growth,reduced hTERT expression,and increasedγ-H2AX protein *** and eosin staining of various organs,including the heart,liver,s

关键词： Esophageal squamous cell carcinoma BIBR1532 Human telomerase reverse transcriptase DNA damage response Telomere-binding proteins

Small cell lung cancer with peripheral neuropathy as the first symptom:Two case reports

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World Journal of Clinical Cases 2025年第9期13卷 30-40页

作者： Man luo xiao-Xi Lu Dan-Yang Meng Jin Hu Department of Neurology Affiliated Hospital of Jiaxing UniversityJiaxing 314000Zhejiang ProvinceChina Department of Information Technology Affiliated Hospital of Jiaxing UniversityJiaxing 314000Zhejiang ProvinceChina

BACKGROUND Small cell lung cancer(SCLC)is the most malignant type of lung *** in the latent period and early stage of the tumor,SCLC is prone to produce distant metastases with complex and diverse clinical *** is most closely related to paraneoplastic syndrome,and some cases present as paraneoplastic peripheral neuropathy(PPN).PPN in SCLC appears early,lacks specificity,and often occurs before diagnosis of the primary *** is easy to be misdiagnosed as a primary disease of the nervous system,leading to missed diagnosis and delayed diagnosis and *** SUMMARY This paper reports two cases of SCLC with limb weakness as the first *** first symptoms of one patient were rash,limb weakness,and abnormal *** patient was repeatedly referred to the hospital for limb weakness and rash for>1 year,during which time,treatment with hormones and immunosuppressants did not lead to significant improvement,and the condition gradually *** patient was later diagnosed with SCLC,and the dyskinesia did not worsen as the dermatomyositis improved after antineoplastic and hormone *** second case presented with limb numbness and weakness as the first symptom,but the patient did not pay attention to ***,the patient was diagnosed with SCLC after facial edema caused by tumor thrombus invading the ***,he was diagnosed with extensive SCLC and died 1 year after *** The two cases had PPN and abnormal electromyography,highlighting its correlation with early clinical indicators of SCLC.

关键词： Paraneoplastic peripheral neuropathy Small cell lung cancer Peripheral neuropathy Electromyography Dermatomyositis Case report

Social function scores and influencing factors in patients with residual depressive symptoms

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World Journal of Psychiatry 2025年第1期15卷 117-127页

作者： Zong-Ling Liao xiao-Li Pu Zhi-Yi Zheng Jie luo Department 1 of Psychiatry Gele Mountain Hospital of Chongqing Mental Health CenterChongqing 400030China Department of Psychology Chongqing Jiangjin District Psychiatric HospitalChongqing 402260China Department of Psychiatry Chongqing Third Social Welfare InstituteChongqing 400036China Women and Children’s Mental Health Center Affiliated Hospital of Chongqing Population and Family Planning Research InstituteChongqing 400020China

BACKGROUND At present,the influencing factors of social function in patients with residual depressive symptoms are still *** depressive symptoms are highly harmful,leading to low mood in patients,affecting work and interpersonal communication,increasing the risk of recurrence,and adding to the burden on *** the influencing factors of their social function is of great *** To explore the social function score and its influencing factors in patients with residual depressive *** This observational study surveyed patients with residual depressive symptoms(case group)and healthy patients undergoing physical examinations(control group).Participants were admitted between January 2022 and December *** functioning was assessed using the Sheehan Disability Scale(SDS),and scores were compared between *** influencing SDS scores in patients with residual depressive symptoms were analyzed by applying multiple linear regression while using the receiver operating characteristic curve,and these RESULTS The SDS scores of the 158 patients with depressive symptoms were 11.48±*** with the control group,the SDS scores and all items in the case group were *** scores were higher in patients with relapse,discon-tinuous medication,drug therapy alone,severe somatic symptoms,obvious residual symptoms,and anxiety scores≥*** history,medication compliance,therapy method,and residual symptoms correlated positively with SDS scores(r=0.354,0.414,0.602,and 0.456,respectively).Independent influencing factors included disease history,medication compliance,therapy method,somatic symptoms,residual symptoms,and anxiety scores(P<0.05).The areas under the curve for predicting social functional impairment using these factors were 0.713,0.559,0.684,0.729,0.668,and 0.628,respectively,with sensitivities of 79.2%,61.8%,76.8%,81.7%,63.6%,and 65.5%and specificities of 83.3%,87.5%,82.6%,83.3%,86.7%,and 92.1%,respectively.C

关键词： Depressive Residual symptoms Social function Influence factors

Prevalence of Helicobacter pylori infection in Chinese military personnel:A cross-sectional,multicenter-based study

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World Journal of Gastroenterology 2025年第3期31卷 42-49页

作者： Han-Chen Min Chun-Yan Zhang Fang-Yu Wang xiao-Hui Yu Shan-Hong Tang Hong-Wu Zhu Ya-Gang Zhao Ji-luo Liu Jian Wang Jing-Han Guo xiao-Mei Zhang Yun-Sheng Yang Chinese People’s Liberation Army Medical School Chinese People’s Liberation Army General HospitalBeijing 100853China Department of Gastroenterology and Hepatology The First Medical CenterChinese People’s Liberation Army General HospitalBeijing 100853China Sixth Healthcare Department The Second Medical CenterChinese People’s Liberation Army General HospitalBeijing 100853China Department of Gastroenterology The 940th Hospital of Joint Logistic Support Force of Chinese People’s Liberation ArmyLanzhou 730050Gansu ProvinceChina Department of Gastroenterology General Hospital of Western Theater CommandChengdu 610011Sichuan ProvinceChina Department of Gastroenterology General Hospital of Southern Theater CommandGuangzhou 510010Guangdong ProvinceChina Department of Epidemiology Naval Medical UniversityShanghai 200433China School of Medicine Nankai UniversityTianjin 300071China National Clinical Research Center for Geriatric Diseases Chinese People’s Liberation Army General HospitalBeijing 100853China

BACKGROUND Helicobacter pylori(***)infection is a prevalent disease encountered in military internal medicine and recognized as the main cause of dyspepsia,gastritis,and peptic ulcer,which are common diseases in military *** guidelines in China state all patients with evidence of active infection with *** are offered ***,the prevalence of *** infection and its regional distribution in the military population remain unclear,which hinders effective prevention and treatment *** the prevalence of *** infection in the military population will aid in the development of customized strategies to better manage this infectious *** To investigate the prevalence of *** infection in the Chinese military population in different geographic *** This multicenter,retrospective study included 22421 individuals from five tertiary hospitals located in north,east,southwest,and northwest cities of *** infection was identified using the urea breath test,which had been performed between January 2020 and December *** Of the 22421 military service members,7416(33.1%)were urea breath *** highest prevalence of *** was in the 30-39 years age group for military personnel,with an infection rate of 34.9%.The majority of infected subjects were younger than 40-years-old,accounting for 70.4%of the infected *** individuals serviced in Lanzhou and Chengdu showed a higher infection prevalence than those in Beijing,Nanjing,and Guangzhou,with prevalence rates of 44.3%,37.9%,29.0%,31.1%,and 32.3%,*** *** infection remains a common infectious disease among military personnel in China and has a relatively high prevalence rate in northwest China.

关键词： Helicobacter pylori Prevalence Military personnel Urea breath test Multicenter study

Antibiotic and glucocorticoid-induced recapitulated hematological remission in acute myeloid leukemia:A case report and review of literature

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World Journal of Clinical Cases 2022年第22期10卷 7890-7898页

作者： xiao-Yun Sun xiao-Dong Yang xiao-Qiu Yang Bo Ju nuan-nuan Xiu Jia Xu Xi-Chen Zhao Department of Hematology The Central Hospital of Qingdao West Coast New AreaQingdao 266555Shandong ProvinceChina Department of Pharmacology The Central Hospital of Qingdao West Coast New AreaQingdao 266555Shandong ProvinceChina

BACKGROUND Leukemic hematopoietic cells acquire enhanced self-renewal capacity and impaired *** emergence of symptomatic leukemia also requires the acquisition of a clonal proliferative *** leukemia patients usually experience an aggressive ***,spontaneous remission occasionally occurs in patients with acute myeloid leukemia(AML),most frequently after recovery from a febrile episode,and this is generally attributed to the triggering of antineoplastic *** may be another explanation for the spontaneous remission as implicated in this *** SUMMARY A 63-year-old Chinese man presented with high fever,abdominal pain and urticaria-like skin *** was diagnosed with AML-M4 with t(8;21)(q22;q22)/RUNX1-RUNX1T1 based on morphological,immunological,cytogenetic and molecular *** had a complex chromosome rearrangement of 48,XY,t(8;21)(q22;q22),+13,+13[9]/49,idem,+mar[9]/49,idem,+8[2].He also had a mutated tyrosine kinase domain in fms-like tyrosine kinase 3 *** was treated with antibiotics and glucocorticoids for gastrointestinal infection and urticaria-like skin *** infection and skin lesions were quickly ***,he achieved hematological remission along with resolution of the febrile episode,gastrointestinal symptoms and skin ***,after relapse,repeating these treatments resulted in a return to hematological ***,he demonstrated strong resistance to antibiotic and glucocorticoid treatment after the second relapse and died of sepsis from bacterial infection with multidrug *** main clinical feature of this patient was that symptomatic AML emerged with flaring of the gut inflammatory disorder and it subsided after resolution of the *** from the present case raises the possibility that in a subgroup of AML patients,the proliferative advantage of leukemia cells may critically require the presence of inflammatory ***

关键词： Acute myeloid leukemia Fms-like tyrosine kinase 3 tyrosine kinase domain Glucocorticoid Antibiotic Spontaneous remission Gastrointestinal infection Case report