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Effect of HSPB9 on Apoptosis of DF-1 Cells

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Biomedical and Environmental Sciences 2019年第2期32卷 107-120页

作者： XU Yong Jie HU Mei Ling ZHOU Liang Hui WANG Qi ZHANG Xi Quan luo qing bin Department of Animal Genetics Breeding and Reproduction College of Animal Science/Key Laboratory of Chicken Genetics Breeding and Reproduction Ministry of Agriculture South China Agricultural University

Objective Our aim was to explore whether heat stress protein(HSP) 9 preferentially expresses under heat stress and affects the expression of other heat stress proteins as well as to explore the effect of HSPB9 overexpression and knockdown on apoptosis in DF-1. Methods We used gene cloning to construct an overexpression vector of the target gene, and synthesized the target gene interference fragment to transfect the chicken fibroblast cell line. Gene and protein expression, as well as apoptosis, were detected by RT-qPCR, Western blot, and flow cytometry. Results Chicken DF-1 cells showed an early state of apoptosis in the early stages of HSPB9 overexpression. In the later stages, as HSPB9 expression increased, the cells showed inhibition of apoptosis. When the cells were under heat stress, HSPB9 expression was much higher and earlier than the expression of HSPB1 and HSPA2. In addition, high expression of HSPB9 had a negative effect on HSPB1 and HSPA2 expression. This negative feedback decreased the percentage of early stages of apoptotic cells and promoted cell survival. Conclusion HSPB9 expression, although rapid, is detrimental to cell survival early during its overexpression. In heat stress, HSPB9 overexpression, while inhibiting the expression of HSPA2 and HSPB1, is beneficial to cell survival.

关键词： Heat stress HSPB9 HSPB1 HSPA2 Apoptosis

The SNPs C.513A>T in the MHC B-F gene and rs15001532 in the SPOCK1 gene are associated with Salmonella pullorum disease resistance in chickens

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Journal of Integrative Agriculture 2016年第8期15卷 1856-1862页

作者： ZHANG Ze-tang GAN Jian-kang ZHANG Wen-wu ZHANG De-xiang ZHANG Xi-quan luo qing-bin College of Animal Science South China Agricultural University Key Lab of Chicken Genetics Breeding and Reproduction Ministry of Agriculture Xinguang Agriculture & Animal Husbandry Co. Ltd.

supported by the Earmarked Fund for the Modern Agroindustry Technology Research System, China （CARS-41）;the National High Technology Research and Development Program of China （2011AA100301）

关键词： MHC B-F gene SPOCK1 gene SNPs Salmonella pullorum partridge chicken

Effect of the C.–1388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

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Journal of Integrative Agriculture 2015年第9期14卷 1808-1815页

作者： ZHANG Wen-wu KONG Li-na ZHANG De-xiang JI Cong-liang ZHANG Xi-quan luo qing-bin College of Animal Science South China Agricultural University Key Laboary of Chicken Genetics Breeding and ReproductionMinistry of Agriculture/South China Agricultural University Guangdong Enterprise Laboary of Healthy Animal Husbandry and Environment Control South China Agricultural University Guangdong Wens Food Group Limited Company

Heat stress is one of the main factors that inlfuence poultry production. Heat shock proteins (HSPs) are known to affect heat tolerance. The formation of HSPs is regulated by heat shock transcription factor 3 (HSF3) in chicken. A DNA pool was established for identifying single nucleotide polymorphisms (SNPs) of the chicken HSF3, and 13 SNPs were detected. The bioinformatic analysis showed that 8 SNPs had the capacity to alter the transcription activity of HSF3. The dual luciferase report gene assay showed that there was a signiifcant difference (PG (S1) and C.–1 388 A>G (S4) sites at the 5′-untranslated region (UTR) of chicken HSF3. The elec-trophoretic mobility shift assay showed that the S4 site was a transcription binding factor. The analysis of the association of the S1 and S4 sites with heat tolerance index revealed that the S4 site was signiifcantly correlated with the CD3+T cel , corticosterone, and T3 levels in Lingshan chickens and with the heterophil/lymphocyte value in White Recessive Rock. These results showed that the S4 site at the 5′ UTR of chicken HSF3 might have an impact on heat tolerance in summer and could be used as a potential marker for the selection of chicken with heat tolerance in the future.

关键词： chicken heat shock factor 3 dual luciferase report gene heat tolerance

Adhesion Performances Between Two Orthotropic Solids Influenced by Temperature Increment

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Acta Mechanica Solida Sinica 2024年第3期37卷 430-443页

作者： qing-Hui luo Yue-Ting Zhou School of Aerospace Engineering and Applied Mechanics Tongji UniversityShanghai200092China

The classical adhesive contact models belong to isothermal adhesion theories,where the effect of temperature on adhesion was ***,a number of experimental results indicated that the adhesion behaviors can be significantly affected by *** this paper,the two-dimensional non-slipping anisothermal adhesion behaviors between two orthotropic elastic cylinders are investigated within the framework of the Johnson-Kendall-Roberts *** stated problem is reduced to the coupled singular integral equations by virtue of the Fourier integral transform,which are solved analytically with the analytical function *** closed-form solutions for the stress fields in the presence of thermoelastic effect are *** stable equilibrium state of contact system is determined by virtue of the Griffith energy *** effect of temperature difference on adhesion behaviors between orthotropic solids is *** is found that the difference between the oscillatory and non-oscillatory solutions increases with increasing the degree of anisotropy of orthotropic *** oscillatory solution cannot be well approximated by the non-oscillatory solution for the orthotropic materials with relatively high anisotropy.

关键词： Adhesive contact Non-slipping JKR model Temperature Griffith energy balance

Switching Behavior of Cascode GaN Under Influence of Gate Driver

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CSEE Journal of Power and Energy Systems 2024年第4期10卷 1816-1833页

作者： bin luo Guangzhao luo Sihai Li School of Automation Northwestern Polytechnical UniversityXi’an 710072China

With high-frequency,low power dissipation and high-efficiency characteristics,Gallium nitride(GaN)power devices are of significant benefit in designing high-speed motor drives,as they improve performance and reduce ***,due to the cascode structure,coupling with the parasitics in gate driver and power circuits,power converters based on cascode GaN are prone to overshoot and oscillate on switching waveforms,which may lead to serious EMC problems,or even device *** complicated structure of cascode GaN device makes the gate driver design comparatively *** analytical model of the switching process considering gate driver parameters is proposed in this *** influence of gate driver parameters on switching behavior is investigated from the perspective of switching speed,waveform overshoot,and power ***-offs among overshoot,switching speed,and power loss are discussed;guidelines to design gate driver parameters are given.

关键词： Gallium nitride(GaN) gate driver oscillation and overshoot switching characteristics

Detecting APT-Exploited Processes through Semantic Fusion and Interaction Prediction

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Computers, Materials & Continua 2024年第2期78卷 1731-1754页

作者： bin luo Liangguo Chen Shuhua Ruan Yonggang luo School of Cyber Science and Engineering Sichuan UniversityChengdu610065China Key Laboratory of Data Protection and Intelligent Management(Sichuan University) Ministry of EducationChengdu610065China Cyber Science Research Institute Sichuan UniversityChengdu610065China

Considering the stealthiness and persistence of Advanced Persistent Threats(APTs),system audit logs are leveraged in recent studies to construct system entity interaction provenance graphs to unveil threats in a ***-based provenance graph APT detection approaches require elaborate rules and cannot detect unknown attacks,and existing learning-based approaches are limited by the lack of available APT attack samples or generally only perform graph-level anomaly detection,which requires lots of manual efforts to locate attack *** paper proposes an APT-exploited process detection approach called ThreatSniffer,which constructs the benign provenance graph from attack-free audit logs,fits normal system entity interactions and then detects APT-exploited processes by predicting the rationality of entity ***,ThreatSniffer understands system entities in terms of their file paths,interaction sequences,and the number distribution of interaction types and uses the multi-head self-attention mechanism to fuse these ***,based on the insight that APT-exploited processes interact with system entities they should not invoke,ThreatSniffer performs negative sampling on the benign provenance graph to generate non-existent edges,thus characterizing irrational entity interactions without requiring APT attack *** last,it employs a heterogeneous graph neural network as the interaction prediction model to aggregate the contextual information of entity interactions,and locate processes exploited by attackers,thereby achieving fine-grained APT *** results demonstrate that anomaly-based detection enables ThreatSniffer to identify all attack *** to the node-level APT detection method APT-KGL,ThreatSniffer achieves a 6.1%precision improvement because of its comprehensive understanding of entity semantics.

关键词： Advanced persistent threat provenance graph multi-head self-attention graph neural network

Gear walk instability caused by brake-disc friction characteristics

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Chinese Journal of Aeronautics 2024年第2期37卷 343-359页

作者： Xiaoqiong DU bin LI Wen CAI Linyin luo School of Aeronautics Northwestern Polytechnical UniversityXi’an 710072China Department of Mechanical Engineering University of MichiganAnn Arbor 48109USA AVIC General Huanan Aircraft Industry Co.Ltd. Zhuhai 519040China

A multi-body dynamic rigid-flexible coupling model of landing gear is established to study the gear walk instability caused by the friction characteristics of the brake *** validating the model with the experimental results,the influence of the landing gear structure and braking system parameters on gear walk is further *** the above factors,the slope of the graph for the friction coefficient of the brake disc and the relative velocity of brake stators and rotors is the most influential factor on gear walk *** trajectory analysis verifies that gear walk occurs when the coupling of multiple factors causes the system to exhibit an equivalent negative damping *** consider a more realistic braking case,a back propagation neural network method is employed to describe the nonlinear behavior of the friction coefficient of the brake *** the realistic nonlinear model of the friction coefficient,the maximum error in predicting the braking torque is less than 10%and the effect of the brake disc temperature on gear walk is *** results reveal that a more negative friction slope may contribute to a more severe unstable gear walk,and reducing the braking pressure is an effective approach to avoid gear walk,which provides help for future braking system design.

关键词： Gear walk Friction characteristics of brake disc Brake-induced vibration Negative damping Rigid-flexible coupling Negative slope of braking torque

Reducing Operation Emissions and Improving Work Efficiency Using a Pure Electric Wheel Drive Tractor

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Engineering 2024年第6期37卷 230-245页

作者： Chang-Kai Wen Wen Ren qing-Zhen Zhu Chun-Jiang Zhao Zhen-Hao luo Sheng-Li Zhang bin Xie Zhi-Jun Meng Research Center of Intelligent Equipment Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China State Key Laboratory of Intelligent Agricultural Power Equipment Beijing 100097China BYD Company Limited Shenzhen 518118China College of Agricultural Engineering Jiangsu UniversityZhenjiang 212013China College of Engineering China Agricultural UniversityBeijing 100083China

In response to the problems of excessive greenhouse-gas and particulate emissions and the low traction efficiency of conventional diesel tractors in the field,a purely electric wheel-side drive tractor was studied,including an electric motor drive system,a battery ballast system,and an electro–hydraulic suspension *** paper develops a dynamics model of an electric tractor-ploughing unit under complex soil conditions,leading to the proposal of an active control method for drive wheel torque and a joint control method for the traction force of the suspension system and the front-and rear-axle loads of a ***,the tractor is prototyped and assembled,and ploughing tests are carried *** ploughing results show that the active torque-distribution control method proposed in this study reduces the tractor slip by 14.83%and increases the traction efficiency by 10.28%compared with the average torquedistribution *** with the conventional traction control mode,the joint control method for traction and ballast proposed in this paper results in a 3.7%increase in traction efficiency,a 15.05%decrease in slip,and a 4.9%reduction in total drive motor energy *** study will help to improve the operation quality and traction efficiency of electric tractors in complex soil conditions.

关键词： Electric tractor Ploughing unit Torque distribution Active ballast Traction performance

Effect of Al_(2)O_(3)fiber on twin intersections-induced dynamic recrystallization in fine-grained TiAl matrix composite

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Journal of Materials Science & Technology 2024年第5期172卷 1-14页

作者： Yaofeng luo Yan Wang Li Wang bin Liu Yong Liu State Key Laboratory of Powder Metallurgy Central South UniversityChangsha 410083China

Dynamic recrystallization(DRX)is of great significance for the thermomechanical processing and microstructural regulation of TiAl ***,the underlying DRX mechanism remains poorly *** this study,an Avrami kinetics model for DRX was established,which was capable of predicting the DRX fraction *** addition,the effect of Al_(2)O_(3)short fiber on the DRX mechanisms of TiAl matrix composite during the isothermal compression was investigated for the first *** re-sults showed that other than inhibiting DRX by particles in the TiAl matrix composites,the addition of Al_(2)O_(3)short fiber accelerated a novel DRX process,which was induced by twinning and twin intersec-tions(TDRX).Thus,this composite exhibited a higher DRX rate than that of the as-cast TiAl monolithic *** origin of the twin intersection and TDRX for the composite was *** stress concentration near the Al_(2)O_(3)fiber was above the critical shear stress for twinning and thus was favorable for the formation of twinning and twin *** high stored strain energy at the regions of twins and twin intersections provided the driving force for *** accelerated the grain refinement in the TiAl matrix near the Al_(2)O_(3)*** present findings would provide a new perspective on DRX mechanisms,and provide the scientific guidance for optimizing the microstructures of TiAl matrix composites.

关键词： TiAl matrix composite Al_(2)O_(3)fiber Twin intersection Dynamic recrystallization