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Transferability of Microsatellite Markers from Brachypodium distachyon to Miscanthus sinensis,a Potential Biomass Crop

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Journal of Integrative Plant Biology 2011年第3期53卷 232-245页

作者： Hua Zhao Jiangyan Yu Frank M. You mingcheng luo Junhua Peng Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture Wuhan Botanical Garden the Chinese Academy of Sciences Wuhan 430074 China Department of Plant Sciences University of California Davis CA 95616 USA Department of Soil and Crop Sciences Colorado State University Fort Collins CO 80523-1170 USA

Miscanthus sinensis has high biomass yield and contributed two of the three genomes in M. x giganteus, a bioenergy crop widely studied in Europe and North America, and thus is a potential biomass crop and an important germplasm for Miscanthus breeding. Molecular markers are essential for germplasm evaluation, genetic analyses and new cultivar development in M. sinensis. In the present study, we reported transferability of simple sequence repeat （SSR） markers from Brachypodium distachyon to M. sinensis. A set of 57 SSR markers evenly distributed across the B. distachyon genome were deliberately designed. Out of these B. distachyon SSR markers, 86.0% are transferable to M. sinensis. The SSR loci amplified in M. sinensis were validated by re-sequencing the amplicons. The polymorphism information content （PIC） of the transferable SSR markers varied from 0.073 to 0.375 with a mean of 0.263, assessed based on 21 M. sinensis genotypes. Phylogenetic tree based on 162 alleles detected by 49 SSR markers could unambiguously distinguish B. distachyon from M. sinensis, and cluster 21 M. sinensis genotypes into three groups that are basically in coincidence with their geographical distribution and ecotype classifications. The markers developed by the comparative genomic approach could be useful for germplasm evaluation, genetic analysis, and marker-assisted breeding in Miscanthus.

关键词： BRACHYPODIUM MISCANTHUS MICROSATELLITES (Genetics) GENETIC markers ENERGY crops GENETIC transformation BIOMASS production

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Valence electronic engineering of superhydrophilic Dy-evoked Ni-MOF outperforming RuO_(2) for highly efficient electrocatalytic oxygen evolution

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Journal of Energy Chemistry 2024年第3期90卷 244-252,I0007页

作者： Zhiyang Huang Miao Liao Shifan Zhang Lixia Wang mingcheng Gao Zuyang luo Tayirjan Taylor Isimjan Bao Wang Xiulin Yang Guangxi Key Laboratory of Low Carbon Energy Materials School of Chemistry and Pharmaceutical SciencesGuangxi Normal UniversityGulin 541004GuangxiChina State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Saudi Arabia Basic Industries Corporation(SABIC)at King Abdullah University of Science and Technology(KAUST) Thuwal 23955-6900Saudi Arabia

Tackling the problem of poor conductivity and catalytic stability of pristine metal-organic frameworks(MOFs) is crucial to improve their oxygen evolution reaction(OER) ***,we introduce a novel strategy of dysprosium(Dy) doping,using the unique 4f orbitals of this rare earth element to enhance electrocatalytic activity of *** method involves constructing Dy-doped Ni-MOF(Dy@Ni-MOF) nanoneedles on carbon cloth via a Dy-induced valence electronic perturbation *** and density functional theory(DFT) calculations reveal that Dy doping can effectively modify the electronic structure of the Ni active centers and foster a strong electronic interaction between Ni and *** resulting benefits include a reduced work function and a closer proximity of the d-band center to the Fermi level,which is conducive to improving electrical conductivity and promoting the adsorption of oxygen-containing ***,the Dy@Ni-MOF achieves superhydrophilicity,ensuring effective electrolyte contact and thus accelerating reaction kinetics,Ex-situ and in-situ analysis results manifest Dy_(2)O_(3)/NiOOH as the actual active ***,Dy@Ni-MOF shows impressive OER performance,significantly surpassing ***,the overall water splitting device with Dy@NiMOF as an anode delivers a low cell voltage of 1.51 V at 10 mA cm^(-2) and demonstrates long-term stability for 100 h,positioning it as a promising substitute for precious metal catalysts.

关键词： Dy@Ni-MOF Dy incorporation Electronic interaction Superhydrophilicity Oxygen evolution reaction

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A bacterial artificial chromosome-based physical map of Manihot esculenta ssp. flabellifolia

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Frontiers of Agricultural Science and Engineering 2016年第4期3卷 321-329页

作者： Yuhua FU Zhiqiang XIA Shujuan WANG Xin CHEN Cheng LU mingcheng luo Hongbin ZHANG Wenquan WANG Institute of tropical Biosciences & Biotechnology Chinese Academy of Tropical Agricultural Sciences Guizhou Institute of Subtropical Crops Guizhou Academy of Agricultural Sciences Department of Plant Sciences UC Davis Department of Soil and Crop Sciences Texas A&M University College Station

Cassava(Manihot esculenta) is known as the third most important food crop in the tropics and also used for industrial feedstock for biofuels. Two new bacterial artificial chromosome(BAC) libraries were constructed for W14(M. Esculenta ssp. flabellifolia), a wild ancestor of domesticated cassava. The libraries were constructed with EcoR I and Hind III insertion vectors, *** EcoR I library has 29952 clones with an average insert size of 115 kb, while the Hind III library consists of 29952 clones with an average insert of 129 kb. The combined libraries contain a total of 59904 clones with an average insert size of 125 kb, representing approximately 10 ?haploid genome equivalents. A total of 29952 clones were fingerprinted and resulted in a cassava physical map composed of 2485 contigs with an average physical length of 336 kb and 2909 singletons, representing approximately762 Mb of the cassava genome. 5000 clones located at the ends of BAC contigs were selected and sequenced. A total of 6077 SNPs and 231 indels were identified, that covered459 gene sequences, of which 6 genes were associated with starch and sucrose metabolism. This BAC-based physical map provides valuable tools to understand the genetics and evolution of cassava.

关键词： cassava BAC library physical map

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CYTOMIXIS AND CELL FUSION OF POLLEN CELLS IN F1 HYBRID OF WHEAT CROSSED WITH RYE, WITH REFERENCE TO THE FORMATION OF ANEUPLOIDS

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四川农业大学学报 1992年第4期 616-622页

作者： mingcheng luo, Chi Yen and Junliang Yang (Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan, China, Zip Code 611830)

Cytomixis refers to the migration of chromatin from one cell to an adjacent one. The cytological investigation demonstrated that cytomixis and direct fusion of two cells occurred in early stage of pollen development, and resulted from genetic controlled behaviour in hybrid plants of wheat (Triticum aestivum L. cv. Xiaoyuer mai) crossed with rye (Secale cereale L. cv. Winter rye). The frequency of occurrences of cytomixis and cell fusion was about 10％. Spontaneous seed settings in the hybrid plants were obtained.

关键词： Cytomixis: Meiosis Aneuploid: Wheat Rye

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THE STUDY ON ESTERASE AND PEROXIDASE ISOZYMES OF WHEAT LANDRACES FROM SHAANXI, CHINA

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四川农业大学学报 1992年第4期 640-645页

作者： Zuli Yang, mingcheng luo and Chi Yen (Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan City, Sichuan, China, Zip Code 611830)

The esterase and peroxidase isozymes of 63 landraces of wheat (Triticum aestivum L.) from Shaanxi province, China have been studied. The results showed as follows:Significant differences of either esterase or peroxidase isozymes were detected in both roots and shoots of seedling among landraces. Eand Ewere special for roots; E, E, E, Eand Eonly appeared in shoots. 2 types of isozyme patterns of esterase were involved in roots, but 4 types in shoots. No variation of peroxidase isozymes appeared in roots, but 3 variations arose in shoots. The roots showed more zymograms and more activity than the shoots in peroxidase isozyme.

关键词： Isozyme Esterase peroxidase Wheat Electrophoresis

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QFP芯片外观视觉检测系统及检测方法

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中国机械工程 2013年第3期24卷 290-294,301页

作者：郑金驹李文龙王瑜辉罗明成华中科技大学数字制造装备与技术国家重点实验室武汉430074

基于QFP芯片的管脚外观检测的需要,开发了基于机器视觉的QFP芯片外观检测系统,并简要介绍了该系统的硬件系统和软件系统。为实现管脚外观检测要求,采用Canny算子边缘检测算法针对QFP管脚的图像进行处理,着重论述了管脚栈高的检测方法及... 详细信息

基于QFP芯片的管脚外观检测的需要,开发了基于机器视觉的QFP芯片外观检测系统,并简要介绍了该系统的硬件系统和软件系统。为实现管脚外观检测要求,采用Canny算子边缘检测算法针对QFP管脚的图像进行处理,着重论述了管脚栈高的检测方法及基于三点法的管脚共面度的检测方法。测试结果表明,该外观检测系统能达到在线生产的工艺要求。

关键词： QFP外观检测机器视觉 Canny算子三点法

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Switching Behavior of Cascode GaN Under Influence of Gate Driver

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CSEE Journal of Power and Energy Systems 2024年第4期10卷 1816-1833页

作者： Bin luo Guangzhao luo Sihai Li School of Automation Northwestern Polytechnical UniversityXi’an 710072China

With high-frequency,low power dissipation and high-efficiency characteristics,Gallium nitride(GaN)power devices are of significant benefit in designing high-speed motor drives,as they improve performance and reduce ***,due to the cascode structure,coupling with the parasitics in gate driver and power circuits,power converters based on cascode GaN are prone to overshoot and oscillate on switching waveforms,which may lead to serious EMC problems,or even device *** complicated structure of cascode GaN device makes the gate driver design comparatively *** analytical model of the switching process considering gate driver parameters is proposed in this *** influence of gate driver parameters on switching behavior is investigated from the perspective of switching speed,waveform overshoot,and power ***-offs among overshoot,switching speed,and power loss are discussed;guidelines to design gate driver parameters are given.

关键词： Gallium nitride(GaN) gate driver oscillation and overshoot switching characteristics

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Precision regulation of plant aromatic amino acid homeostasis

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Science China(Life Sciences) 2024年第6期67卷 1316-1317页

作者： Jie luo Yazhouwan National Laboratory(YNL) Sanya 572025China School of Breeding and Multiplication(Sanya Institute of Breeding and Multiplication) Hainan UniversitySanya 572025China

The aromatic amino acids(AAAs)phenylalanine(Phe),tyrosine(Tyr),and tryptophan(Trp)are not only building blocks of proteins,but also precursors of numerous primary and specialized metabolites(Schenck and Maeda,2018).For instance,Trp is a precursor of the phytohormone auxin and the plant defense compounds indole glucosinolates,while Phe serves as a precursor of lignin,coumarin and *** plant natural products such as morphine and vitamin E are derived from Tyr,and have been employed in human medicine and nutrition(Schenck and Maeda,2018;Wu et al.,2022;Yokoyama et al.,2021).

关键词： aromatic instance coumarin

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In Situ Polymer Gel Electrolyte in Boosting Scalable Fibre Lithium Battery Applications

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Nano-Micro Letters 2024年第11期16卷 170-173页

作者： Jie luo Qichong Zhang Key Laboratory of Multifunctional Nanomaterials and Smart Systems Chinese Academy of SciencesSuzhou Institute of Nano-Tech and Nano-Bionics398 Ruoshui RoadSuzhou 215123People’s Republic of China

The poor interfacial stability not only deteriorates fibre lithium-ion batteries(FLBs)performance but also impacts their scalable *** efficiently address these challenges,*** Peng team proposed a generalized channel structures strategy with optimized in situ polymerization technology in their recent *** resultant FLBs can be woven into different-sized powering textiles,providing a high energy density output of 128 Wh kg^(-1) and simultaneously demonstrating good durability even under harsh *** a promising strategy expands the horizon in developing FLB with particular polymer gel electrolytes,and significantly ever-deepening understanding of the scaled wearable energy textile system toward a sustainable future.

关键词： High-performance fibre lithium batteries Gel electrolytes Channel structures Stable interface Scalable application

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Catalyst design and structure control for photocatalytic refineries of cellulosic biomass to fuels and chemicals

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Journal of Energy Chemistry 2024年第7期94卷 102-127页

作者： Lulu Sun Nengchao luo State Key Laboratory of Catalysis Dalian National Laboratory for Clean EnergyDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian 116023LiaoningChina

Lignocellulosic biomass is the largest renewable hydrocarbon resource on *** cellulose,one of the major components of lignocellulose,powered by solar energy is a promising way of providing lowcarbon-footprint energy chemicals such as H_(2),HCOOH,CO,and transportation ***-of-the-art biorefineries target the full use of biomass feedstocks as they have a maximum collection radius of 75-100 km,requesting efficient and selective photocatalysts that significantly influence the outcome of photocatalytic ***-performed photocatalysts can harvest a broad solar spectrum and are active in breaking the chemical bonds of cellulose,decreasing the capital investments of ***,photocatalysts should control the selectivity of cellulose conversion,originating target products to level down separation *** separation in photocatalysts and interfacial charge transfer between photocatalysts and cellulose affect the activity and selectivity of cellulose refineries to H2 and carbonaceous *** account for the challenges above,this review summarizes photocatalysts for the refineries of cellulose and downstream platform molecules based on the types of products,with the structure features of different types of photocatalysts discussed in relation to the targets of either improving the activity or product *** addition,this review also sheds light on the methods for designing and regulating photocatalyst structures to facilitate photocatalytic refineries of cellulose and platform molecules,meanwhile summarizing proposed future research challenges and opportunities for designing efficient photocatalysts.

关键词： Photocatalysts Biorefineries Cellulose Fuels Hydrogen

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