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Aβ-Carotene Ketolase Gene NfcrtO from Subaerial Cyanobacteria Confers Drought Tolerance in Rice

Rice science 2024年第1期31卷 62-76页

作者： GAO Ningning YE Shuifeng ZHANG Yu ZHOU Liguo MA Xiaosong YU Hanxi LI Tianfei HAN Jing LIU Zaochang luo lijun College of Plant Science and Technology Huazhong Agricultural UniversityWuhan 430070China Shanghai Agrobiological Gene Center Shanghai 201106China College of Life Sciences Shangrao Normal UniversityShangrao 334001China Institute for Agri-food Standards and Testing Technology Shanghai Academy of Agricultural SciencesShanghai 201403China

Nostoc flagelliforme is a terrestrial cyanobacterium that can resist many types of stressors,including drought,ultraviolet radiation,and extreme temperatures.In this study,we identified the drought tolerance gene NfcrtO,which encodes aβ-carotene ketolase,through screening the transcriptome of N.flagelliforme under water loss stress.Prokaryotic expression of NfcrtO under 0.6 mol/L sorbitol or under 0.3 mol/L NaCl stress significantly increased the growth rate of Escherichia coli.When NfcrtO was heterologously expressed in rice,the seedling height and root length of NfcrtO-overexpressing rice plants were significantly higher than those of the wild type(WT)plants grown on½Murashige and Skoog solid medium with 120 mmol/L mannitol at the seedling stage.Transcriptome analysis revealed that NfcrtO was involved in osmotic stress,antioxidant,and other stress-related pathways.Additionally,the survival rate of the NfcrtO-overexpression lines was significantly higher than that of the WT line under both hydroponic stress(24%PEG and 100 mmol/L H_(2)O_(2))and soil drought treatment at the seedling stage.Physiological traits,including the activity levels of superoxide dismutase,peroxidase,catalase,total antioxidant capacity,and the contents of proline,trehalose,and soluble sugar,were significantly improved in the NfcrtO-overexpression lines relative to those in the WT line under 20%PEG treatment.Furthermore,when water was withheld at the booting stage,the grain yield per plant of NfcrtO-overexpression lines was significantly higher than that of the WT line.Yeast two-hybrid analysis identified interactions between NfcrtO and Dna J protein,E3 ubiquitin-protein ligase,and pyrophosphate-energized vacuolar membrane proton pump.Thus,heterologous expression of NfcrtO in rice could significantly improve the tolerance of rice to osmotic stress,potentially facilitating the development of new rice varieties.

关键词： antioxidant enzyme β-carotene ketolase drought resistance Nostoc flagelliforme osmotic stress rice transcriptome analysis

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Genetic Dissection of Quantitative Trait Loci for Panicle Traits and Heat Tolerance by High-Density Bin Map in Rice

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Rice science 2022年第6期29卷 507-511,I0007-I0020页

作者： LIU Hongyan MA Xiaosong LI Enxi ZENG Xianjun luo lijun Shanghai Agrobiological Gene Center Shanghai 201106China Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization Ministry of Agriculture and Rural AffairsShanghai 201106China Hainan University Haikou 570228China Huazhong Agricultural University Wuhan 430070China

A recombinant inbred line(RIL)with 179 lines derived from a cross between a heat tolerant line Huhan 1B and a heat sensitive line Hanhui 3 was used for QTL mapping for panicle traits and heat tolerance.A total of 52 QTLs were identified with LOD scores from 3.85 to 54.93,each explaining 0.70%to 21.48%of the phenotypic variances,including 11 for panicle length(PL),11 for seed number per panicle(SN),14 for spikelet number per panicle(SNPP),6 for seed-setting rate(SSR)under normal conditions,and 5 for seed-setting rate(SSR_HT)and 5 for relative seed-setting rate(RSSR)under heat stress,respectively.

关键词： panicle Traits Map

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Dissecting Genetic Basis of Deep Rooting in Dongxiang Wild Rice

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Rice science 2022年第3期29卷 277-287页

作者： NIE Yuanyuan XIA Hui MA Xiaosong LOU Qiaojun LIU Yi ZHANG Anling CHENG Liang YAN Longan luo lijun Huazhong Agricultural University Wuhan 430070China Shanghai Agrobiological Gene Center Shanghai 201106China Jiangxi Research and Development Center of Super Rice/Nanchang Branch of Chinese National Center for Rice Improvement Nanchang 330200China

Deep rooting is an important trait in rice drought resistance.Genetic resources of deep-rooting varieties are valuable in breeding of water-saving and drought-resistant rice.In the present study,234BC2F7 backcross introgression lines were derived from a cross of Dongye 80(an accession of Dongxiang wild rice as the donor parent)and R974(an indica restorer line as the recurrent parent).A genetic linkage map containing 1977 bin markers was constructed by ddRADSeq for QTL analysis.Thirty-one QTLs for four root traits(the number of deep roots,the number of shallow roots,the total number of deep roots and the ratio of deep roots)were assessed on six rice chromosomes in two environments(2020 Shanghai and 2021 Hainan).Two of the QTLs,qDR5.1 and qTR5.2,were located on chromosome 5 in a 70-kb interval.They were detected in both environments.qDR5.1 explained 13.35%of the phenotypic variance in 2020 Shanghai and 12.01%of the phenotypic variance in 2021 Hainan.qTR5.2 accounted for 10.88%and 10.93%of the phenotypic variance,respectively.One QTL(qRDR2.2)for the ratio of deep roots was detected on chromosome 2 in a 210-kb interval and accounted for 6.72%of the phenotypic variance in 2020.The positive effects of these three QTLs were all from Dongxiang wild rice.Furthermore,nine and four putative candidate genes were identified in qRDR2.2 and qDR5.1/qTR5.2,respectively.These findings added to our knowledge of the genetic control of root traits in rice.In addition,this study will facilitate the future isolation of candidate genes of the deep-rooting trait and the utilization of Dongxiang wild rice in the improvement of rice drought resistance.

关键词： Dongxiang wild rice backcross introgression line deep rooting genetic analysis QTL

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尊重教师的价值意蕴与历史逻辑

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教师教育研究 2024年第3期36卷 13-19页

作者：罗丽君王皇星西南大学教师教育学院重庆400715

尊重教师是一种主客体之间的对象性关系,涉及注意、遵从、积极评价与适宜的行为举止四个要素,帮助尊重主体克服自爱和自大的主观偏好,客观认识自身片面性与人类局限性;作为来自他人的重要社会支持,满足尊重客体基本心理需要,减轻塑造灵... 详细信息

尊重教师是一种主客体之间的对象性关系,涉及注意、遵从、积极评价与适宜的行为举止四个要素,帮助尊重主体克服自爱和自大的主观偏好,客观认识自身片面性与人类局限性;作为来自他人的重要社会支持,满足尊重客体基本心理需要,减轻塑造灵魂、塑造生命、塑造人的情绪工作可能带来的不良心理反应,具有“尊则谦谦”与“尊则自许”的主客体价值。从知识社会学的视角分析,尊重教师作为文化基因承载着中华民族“尊老”“重道”“循礼”的集体记忆,从一种“互动模式”到“不言自明”的社会规范或秩序,从生成经验性“知识”到传承为一种“客观现实”,逻辑上经历了惯习化、制度化与合法化的过程。

关键词：尊重教师价值意蕴历史逻辑

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青少年感知教师公正性如何影响校园欺凌行为?——基于认知——情感系统理论的结构方程建模

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中国远程教育 2024年第2期44卷 24-35页

作者：罗丽君蒙琪缘赵玉芳西南大学教师教育学院重庆400715 西南大学重庆400715

校园欺凌是青春期和成年早期反社会行为、暴力犯罪的显著预测因素,学校是防治校园欺凌的关键阵地,而教师扮演着预防者与干预者的双重角色。青少年感知教师公正性能否降低欺凌行为,又是如何影响校园欺凌的?研究者尝试基于认知—情感系统... 详细信息

校园欺凌是青春期和成年早期反社会行为、暴力犯罪的显著预测因素,学校是防治校园欺凌的关键阵地,而教师扮演着预防者与干预者的双重角色。青少年感知教师公正性能否降低欺凌行为,又是如何影响校园欺凌的?研究者尝试基于认知—情感系统理论,探究青少年感知教师公正性对校园欺凌的影响以及学校归属感、道德推脱在其中的作用,通过对1,077名中学生的调查构建了结构方程模型。研究发现,感知教师公正性越高,学生实施校园欺凌的可能性越低,感知教师公正性对校园欺凌的影响通过学校归属感(情感)与道德推脱(认知)的链式中介作用得以实现。校园欺凌实际上是一种恃强凌弱的不公正、不道德的群体过程,学校归属感使得学生愿意遵守校规班纪等指令性规范,但是当感知具有群体原型领导者特征的教师不公正时,学生会将其作为描述性规范的标准,而将不公正辩护为群体内的普遍行为,激活将责任归咎于权威或强调群体决策的道德推脱机制进而实施校园欺凌。

关键词：校园欺凌感知教师公正性学校归属感道德推脱

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在线学习环境中青少年感觉寻求对学习倦怠的影响机制

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中国电化教育 2024年第6期 108-116页

作者：罗丽君文燕陈冰赵玉芳西南大学教师教育学院重庆400715 西南大学教育学部重庆400715 西南大学心理学部重庆400715

学习倦怠不仅会影响青少年学业成就,还可能导致学业拖延、情绪障碍、睡眠障碍甚至自残自杀等严重心理或行为问题。在线学习环境中青少年感觉寻求会影响学习倦怠吗?又是如何影响学习倦怠的?该文基于“人-环境”契合理论,探究感觉寻求对... 详细信息

学习倦怠不仅会影响青少年学业成就,还可能导致学业拖延、情绪障碍、睡眠障碍甚至自残自杀等严重心理或行为问题。在线学习环境中青少年感觉寻求会影响学习倦怠吗?又是如何影响学习倦怠的?该文基于“人-环境”契合理论,探究感觉寻求对学习倦怠的影响以及短视频过度使用、焦虑在其中的作用,通过对1563名中学生的调查构建了结构方程模型。研究发现,感觉寻求水平越高,学习倦怠的程度越高;短视频过度使用和焦虑均可在感觉寻求对学习倦怠的影响中起单独中介作用,并通过“短视频过度使用→焦虑”路径发挥链式中介作用。在线学习环境难以达到和维持感觉寻求个体较高的最佳唤醒水平,因而驱使个体在有限的时空内将注意力向外转移到短视频使用中,导致焦虑进而产生学习倦怠。应预防和减少感觉寻求的不利影响,优化在线学习环境以减少学习倦怠。

关键词：学习倦怠感觉寻求短视频过度使用焦虑

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冷冻干燥法制备改性电石渣同时脱硫脱硝研究

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环境科学学报 2024年第5期44卷 423-434页

作者：罗绍君李慧贾丽娟冯嘉予罗利军高冀芸王访云南民族大学化学与环境学院昆明650504 昆明理工大学环境科学与工程学院昆明650500

为了进一步提高对电石渣的资源化利用,本文以电石渣为原料,采用碳酸氢钾(KHCO_(3))浸渍与冷冻干燥法相结合制备改性电石渣用于烟气同时脱硫脱硝.结果表明与普通烘干相比,采用冷冻干燥法制备的改性电石渣具有更高的脱除效果,在反应前90 ... 详细信息

为了进一步提高对电石渣的资源化利用,本文以电石渣为原料,采用碳酸氢钾(KHCO_(3))浸渍与冷冻干燥法相结合制备改性电石渣用于烟气同时脱硫脱硝.结果表明与普通烘干相比,采用冷冻干燥法制备的改性电石渣具有更高的脱除效果,在反应前90 min内二氧化硫(SO_(2))脱除率保持在100%,SO_(2)脱除的有效时间增加15 min;一氧化氮(NO)的脱除效率提高15%,有效时间增加35 min,反应活化能降低5.69 kJ·mol^(-1).材料的SO_(2)和NO吸附容量分别提高到1.18和2.05倍.通过物理化学表征分析发现:采用冷冻干燥法制备的材料其主要的活性物质C=O含量明显上升,孔径得以提高,并且钾元素也分布更加均匀,更有利于改性电石渣脱除SO_(2)/NO.

关键词：冷冻干燥法电石渣 KHCO_(3) 同时脱除SO_(2)/NO

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Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice

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Chinese Science Bulletin 2012年第4期57卷 336-343页

作者： YE ShuiFeng YU ShunWu SHU LieBo WU JinHong WU AiZhong luo lijun Shanghai Agrobiological Gene Center Shanghai 201106China School of Agriculture and Biology Shanghai Jiaotong UniversityShanghai 201101China Shanghai Academy of Agricultural Sciences Shanghai 201106China

Plant heat shock proteins (Hsps) facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of rice cv. IRAT 109 leaf samples at the seedling stage under drought stress. To obtain additional information on the 9 OsHsp genes, this study focused on an expression profile analysis at different development stages throughout the life cycle of rice, and under different abiotic stresses and phyto-hormone treatments. The 9 genes exhibited distinctive expression patterns in different organs or development stages. Five of the genes (OsHsp72.90, OsHsp72.57, OsHsp71.18, OsHsp24.15 and OsHsp18.03) showed high expression in the endosperm, indicating that OsHsp genes may play important roles in rice seed development. All 9 OsHsps were up-regulated under heat, polyethylene glycol, and abscisic acid treatment, whereas salt stress caused up-regulation of 6 genes (OsHsp93.04, OsHsp71.10, OsHsp71.18, OsHsp72.57, OsHsp24.15 and OsHsp18.03) and cold stress resulted in down-regulation of OsHsp93.04 and OsHsp72.57. These diverse expression profiles imply potential functional diversity of the Hsp gene family in rice.

关键词：表达谱分析热休克蛋白蛋白基因水稻品种生命周期逆境蛋白质折叠蛋白质组分

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Integrated phenotypic,phylogenomic,and evolutionary analyses indicate the earlier domestication of Geng upland rice in China

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Molecular Plant 2022年第10期15卷 1506-1509页

作者： Zhi luo Hui Xia Zhigui Bao Lei Wang Yu Feng Ting Zhang Jie Xiong Liang Chen lijun luo College of Plant Sciences&Technology Huazhong Agricultural UniversityWuhanChina Shanghai Agrobiological Gene Center ShanghaiChina Shenzhen Branch Guangdong Laboratory of Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenChina CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengduChina Key Laboratory of Grain Crop Genetic Resources Evaluation and Utlization Ministry of Agriculture and Rural AffairsShanghaiChina Shanghai OE Biotech.Co. Ltd.ShanghaiChina School of Agriculture and Biology Shanghai Jiao Tong UniversityShanghaiChina

Dear Editor,Ensuring food security with finite resources and environmental impacts is a new challenge in sustainable agriculture(Yu et al.,2022).It is therefore essential to develop suitable rice varieties for resource-saving and environmentally friendly cultivation(luo et al.,2019;Yu et al.,2022).Upland rice,which evolves many green characters for rice sustainable cultivation(e.g.,drought resistance,the usage efficiency of nitrate,the good capacity of direct seeding,etc.),is therefore an important germplasm in rice breeding(luo et al.,2019).However,the origination and evolution of upland rice,as well as its green genetic resource,remain mysterious(Xia et al.,2019).

关键词： breeding drought upland

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Experimental validation of inter-subspecific genetic diversity in rice represented by the differences between the DNA sequences of 'Nipponbare' and '93-11'

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Chinese Science Bulletin 2007年第10期52卷 1327-1337页

作者： MEI HanWei FENG FangJun LU BaoRong WEN WeiWei Andrew H. PATERSON CAI XingXing CHEN Liang Frank A. FELTUS XU XiaoYan WU JingHong YU XinQiao CHEN HongWei LI Ying luo lijun Shanghai Agrobiological Gene Center Shanghai 201106 China Institute of Biodiversity Science Fudan University Shanghai 200433 China Huazhong Agricultural University Wuhan 430070 China Plant Genome Mapping Laboratory University of Georgia Athens GA30602 USA

The pedigrees of three sequenced rice cultivars were analyzed to show that a majority of the genetic composition of 'Nipponbare' originates from japonica cultivars while the minority originates from indica cultivars. In contrast, '93-11' is derived mainly from indica cultivars with a smaller contribution from japonica cultivars. All ancestors of 'Guang lu ai 4' appeared to be indica lines. A set of molecular markers (46 InDels and 53 SSRs) polymorphic between 'Nipponbare' and '93-11' were examined in 46 typical indica and 47 typical japonica cultivars selected from 443 accessions according to Cheng's index. All cultivars were divided into indica and japonica groups without overlapping when clustered by Cheng's index, InDels and SSRs. Much higher InDel and SSR diversity between groups than within groups implies that the marker polymorphisms between 'Nipponbare' and '93-11' represent a large proportion of inter-subspecific diversity. About 85% of indica cultivars and more than 90% of japonica cultivars were confirmed to have the same PCR banding patterns as '93-11' and 'Nipponbare', respectively. Some polymorphic loci between 'Nipponbare' and '93-11' cannot be validated in other indica and japonica cultivars, either as subspecies-specific but not predominant alleles, or alleles not specific between the two groups. It was concluded that molecular markers developed from sequence polymorphism between 'Nipponbare' and '93-11' often represent inter-subspecific diversity, although some exceptions were sensitive to either particular marker loci or particular cultivars.

关键词： 93-11水稻日本晴稻亚种间遗传多态性 DNA序列实验验证

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