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Journal of Integrative Agriculture 2014年第6期13卷 1250-1257页

作者： ZHANG Shuai LI Jing Lü Li-min WANG Chun-yi luo jun-yu CUI Jin-jie State Key Laboratory of Cotton Biology/Institute of Cotton Research Chinese Academy of Agricultural Sciences

Plants reshape their transcriptomes, proteomes and metabolomes in response to insect damage. In this study, we used suppression subtractive hybridization to investigate the transcriptomes of two cotton varieties （CCRI41 and CCRI23） under Apolygus lucorum damage. From the CCRI23 libraries we obtained 92 transcripts and from the CCRI41 libraries we obtained 96 transcripts. 26 and 63 of the transcripts from CCRI23 and CCRI41, respectively, had known functions. Using reverse transcription PCR, we detected expression proifle of genes with known functions. Ultimately, we identiifed eight signiifcantly regulated genes, including one downregulated and four upregulated genes from the CCRI41 libraries, and one downregulated and two upregulated genes from the CCRI23 libraries. Only the gene encoding the polyphenol oxidase （PPO） is involved in plant defense against insect herbivores, and the others are related to improving tolerance to insect damage. Quantitative real-time PCR was used to study changes in expression levels during A. lucorum damage in CCRI23 and CCRI41. Signiifcantly regulated genes from CCRI23 showed a response in CCRI23 but not response in CCRI41. Similarly, signiifcantly regulated genes from CCRI41 showed a response in CCRI41 but not response in CCRI23. The results showed that, among transcriptomes of cotton varieties, there are different responses to A. lucorum damage.

关键词： suppression subtractive hybridization Apolygus lucorum defense against insect herbivores

Molecular Characterization and SYBR Green I-Based Quantitative PCR for Duck Hepatitis Virus Type 1

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Agricultural Sciences in China 2008年第9期7卷 1140-1146页

作者： luo yu-jun ZHANG Gui-hong XU Xiao-qin CHEN Jian-hong LIAO Ming College of Veterinary Medicine South China Agricultural University Guangzhou 510642 P.R.China College of Life and Science Foshan University Guangzhou 528231 P.R.China

To determine the genomic sequence of a duck hepatitis virus type 1 （DHV-1） strain, real-time quantitative polymerase chain reaction （RTQ-PCR） assay based on SYBR Green I technology was developed to target 3D gene of DHV-1, Comparative sequence analysis showed that the genome has a typical picornarivus genetic organization, and strain DHV-1 R genetic organaiztion is 5＇ untranslated region （UTR）-VP0-VP3-VPI-2A1-2A2-2B-2C-3A-3B-3C-3D-3＇ UTR, DHV-1 R has close relationship with Parechovirus, and has 95.1-99.1% nucleotide sequence identity with other DHV-1 strains. Based on the DHV-1 sequences in GenBank, three pairs of specific primers were designed to amplify DHV-1 using real-time PCR. The results showed that real-time PCR Tm value is 85.6℃ and the real-time PCR provides a broad dynamic range, detecting from 102 to 109 copies of DHV-1 cDNA per reaction. No cross-reactions were found in specimens containing DPV, AIV and NDV. It is concluded that DHV-1 belongs to a new group of the family Picornaviridae that may form a separate genus most closely related to the genus Parechovirus. All results showed that the real-time PCR has high sensitivity and specificity to detect DHV-1 using SYBR Green I dissociation curve analysis, isolates can be distinguished by their melting temperature. These methods are rapid, sensitive, and reliable, and can be readily adapted for detection of DHV-1 from other clinical samples.

关键词： duck hepatitis virus type 1 complete genome real-time RT-PCR

The biotypes and host shifts of cotton-melon aphids Aphis gossypii in northern China

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Journal of Integrative Agriculture 2018年第9期17卷 2066-2073页

作者： ZHANG Shuai luo jun-yu WANG Li WANG Chun-yi LU Li-min ZHANG Li-juan ZHU Xiang-zhen CUI Jin-jie State Key Laboratory of Cotton Biology Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyang 455000P.R.China

Aphis gossypfi is a globally distributed species and therefore has a highly variable life cycle. Populations of A. gossypii in northern China exhibit greater genotypic diversity and a broader host range, yet the details of life cycles of different biotypes is still unclear. In this study, the Cytb and 16S gene regions of A. gossypfi collected from 5 common summer hosts and 4 primary hosts were analyzed. A total of 57 haplotypes were obtained from 1 046 individual A. gossyp# sequences. The sequence included 44 variable sites, 27 of which were parsimony informative sites and 17 of which were singleton variable sites. The most frequent 3 haplotypes were found in 896 individuals, representing a total of 85.7% of all individuals and 36 haplotypes were found in 1 individual. A neighbor-joining tree was constructed using 21 haplotypes that were found in more than 2 individuals. Considering the individual host plant, 5 biotypes were identified. Type 1 corresponded exactly to the cucurbit host-race and the other 4 biotypes were found as cotton host-races. Type 3 was the most abundant biotype in cotton fields in northern China.

关键词： cotton-melon aphid Cytb and 16S genes haplotype biotype cotton field

Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fields

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Journal of Integrative Agriculture 2017年第7期16卷 1624-1633页

作者： luo jun-yu ZHANG Shuai ZHU Xiang-zhen LU Li-min WANG Chun-yi LI Chun-hua CUI Jin-jie ZHOU Zhi-guo State Key Laboratory of Cotton Biology Institute of Cotton Research Chinese Academy of Agricultural Sciences College of Agriculture Nanjing Agricultural University/Key Laboratory of Crop Physiology & Ecology in Southern China Ministry of Agriculture

With increased cultivation of transgenic Bacillus thuringiensis （Bt） cotton in the saline alkaline soil of China, assessments of transgenic crop biosafety have focused on the effects of soil salinity on rhizosphere microbes and Bt protein residues. In 2013 and 2014, investigations were conducted on the rhizosphere microbial biomass, soil enzyme activities and Bt protein contents of the soil under transgenic Bt cotton （variety GK19） and its parental non-transgenic cotton （Simian 3） cultivated at various salinity levels （1.15, 6.00 and 11.46 dS m-1）. Under soil salinity stress, trace amounts of Bt proteins were ob- served in the Bt cotton GK19 rhizosphere soil, although the protein content increased with cotton growth and increased soil salinity levels. The populations of slight halophilic bacteria, phosphate solubilizing bacteria, ammonifying bacteria, nitrifying bacteria and denitrifying bacteria decreased with increased soil salinity in the Bt and non-Bt cotton rhizosphere soil, and the microbial biomass carbon, microbial respiration and soil catalase, urease and alkaline phosphatase activity also decreased. Correlation analyses showed that the increased Bt protein content in the Bt cotton rhizosphere soil may have been caused by the slower decomposition of soil microorganisms, which suggests that salinity was the main factor influencing the relevant activities of the soil microorganisms and indicates that Bt proteins had no clear adverse effects on the soil microorganisms. The results of this study may provide a theoretical basis for risk assessments of genetically modified cotton in saline alkaline soil.

关键词： soil salinity Bt cotton soil microorganisms microbial biomass carbon microbial respiration soil enzyme activityBt protein

Effects of HBV X gene and arsenic trioxide on the expression of p53 in cultured HepG2 cells

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Chinese Medical Journal 2007年第24期120卷 2181-2184页

作者： LEI Jian-hua HE Xing-e YANG Xu ZHANG Min LIAN jun luo Hong-yu WANG Wen-long Liver Disease Research Center Second Xiangya Hospital CentralSouth University Changsha 410011 China

Background Hepatitis B virus （HBV） X protein （HBx） and p53 could mutually down-regulate at transcriptional level and HBx could bind with p53 protein within its transactivation domain and inhibit the function of p53 protein. In recent years, effects of arsenic trioxide （As2O3） on the expression of p53 protein have been widely studied, while little is known about the activity of p53 protein. This study was undertaken to delineate the effect of HBV X gene and AS203 on p53 protein expression （level and activity） in HepG2 cells by small hairpin RNA （shRNA）-mediated RNA interference （RNAi） technique. Methods Cell line HepG2 and cells with stable expression of HBV X gene （HepG2-X） were treated with 2 pmol/L AS203, with corresponding untreated cells serving as controls. Cell lysates and nuclear extracts were extracted. Total level and the relative activity of p53 protein were detected by modified enzyme-linked immunosorbent assay （ELISA）. HBV X gene sequence-specific shRNA expression vector （pXi-1 and pXi-2） and sequence-unrelated control （pXi-3） were transfected into HepG2-X. Single cell clone with stable expression of shRNA was selected and exposed to propagating culture. The effect of As2O3 on p53 protein expression and activity was re-observed. Results Total p53 protein level was up-regulated and its relative activity ratio was enhanced by As2O3 in HepG2 and HepG2-X cells. The total p53 protein level induced by As2O3 was up-regulated by HBV X gene expression, while its relative activity was significantly suppressed. The suppression was removed after HBV X gene expression was repressed by shRNA. Conclusions As2O3 up-regulates p53 protein expression and enhance its activity. HBV X up-regulates As2O3 induced-p53 protein expression while suppresses its activity.

关键词： hepatitis B virus gene X arsenic trioxide RNA interference tumor suppressor protein p53

Tilting homeostatic and dyshomeostatic microglial balance in health and disease:transforming growth factor-beta1 as a critical protagonist

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Neural Regeneration Research 2025年第10期20卷 2895-2897页

作者： Nicolas Hugues yu luo Department of Molecular and Cellular Biosciences University of CincinnatiCincinnatiOHUSA Neuroscience Graduate Program University of CincinnatiCincinnatiOHUSA Division of Immunobiology Cincinnati Children’s Hospital Medical CenterUniversity of Cincinnati College of MedicineCincinnatiOHUSA

Adult microglia,by continuously sensing changes in their environment and communicating with nearly all brain cell types,are considered to be the immune sentinels of the *** the healthy central nervous system(CNS),microglia display a unique molecular homeostatic signature(i.e.,Tmem119,P2ry12,Sall1,Siglech,Gpr34,and Hexb)(Figure 1A).

关键词： Figure signature critical

基于逆向光线跟踪与图像绘制的运动水面反射场景绘制

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计算机科学 2014年第S1期41卷 181-183页

作者：李纲罗玉军川北医学院基础医学院计算机教研室南充637007

提出了一种结合图像绘制和逆向光线跟踪的绘制算法来生成水面反射场景。首先,采用逆向光线跟踪方法获得视点所见水面反射场景点;然后在反射光线与场景相交点求解时,采用场景图像平面搜索,并将搜索到的图像平面象素点反向投影到观察坐标... 详细信息

提出了一种结合图像绘制和逆向光线跟踪的绘制算法来生成水面反射场景。首先,采用逆向光线跟踪方法获得视点所见水面反射场景点;然后在反射光线与场景相交点求解时,采用场景图像平面搜索,并将搜索到的图像平面象素点反向投影到观察坐标系求取光线与场景物体相交点以获取反射物颜色;最后按照Fresnel公式计算获得视点所见的水面上某点的颜色,通过对整个图像平面进行遍历,获得水面反射场景。使用该方法绘制的水面反射场景能够物理真实地模拟水面反射效果。对于波动水面附近或漂浮于水面上物体的反射场景,该方法较其它方法能够更好地获得物理真实的绘制结果。

关键词：水面反射图像绘制逆向光线跟踪

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柴油机工作过程模拟计算

船海工程 2006年第1期35卷 15-18页

作者：罗玉君陈辉孙俊武汉理工大学能动学院武汉430063

对大型船用柴油机缸内工作过程进行了数学建模与仿真,并运用Visual C++编制了柴油机工作过程模拟程序,对MAN B&W 6L70MCE柴油机工作过程进行了模拟计算,获得了缸内示功图,模拟计算与试验结果对比说明该模拟计算程序的正确性和实用性。

关键词：柴油机工作过程计算与仿真

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网上参考咨询服务的基本方法与技巧

上海交通大学学报 2003年第S1期37卷 67-69,73页

作者：罗宇君广东省立中山图书馆广州市510110

从社会需要和图书馆自身变化两方面考虑 ,数字图书馆需要加强参考咨询服务 .针对数字图书馆的特征 ,结合作者在网上咨询工作中的实践经验。

关键词：数字图书馆参考咨询服务方法

Effect of cholesterol metabolism on hepatolithiasis

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World Journal of Gastroenterology 2025年第1期31卷 157-162页

作者： Lin Zheng Zi-yu Ye jun-Ji Ma Department of Gastroenterology The Second Hospital of Hebei Medical UniversityHebei Key Laboratory of GastroenterologyHebei Institute of GastroenterologyHebei Clinical Research Center for Digestive DiseasesNo.215 Heping West RoadShijiazhuang 050000Hebei ProvinceChina

Surgical intervention is currently the primary treatment for hepatolithiasis;how-ever,some patients still experience residual stones and high recurrence rates after *** metabolism seems to play an important role in hepatoli-thiasis pathogenesis.A high cholesterol diet is one of the significant reasons for the increasing incidence of ***,regular diet and appropriate medical intervention are crucial measures to prevent hepatolithiasis and reduce recurrence rate after *** dietary cholesterol and drugs that increase cholesterol stone solubility are key therapeutic approaches in treating *** article discusses the cholesterol metabolic pathways related to the pathogenesis of hepatolithiasis,as well as food intake and targeted therapeutic drugs.

关键词： Hepatolithiasis Cholesterol metabolism High-fat diet 3-hydroxy-3-methylglutaryl-coenzyme A reductase Interlobular bile duct