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Erratum to:DNA barcoding of the fungal genus Neonectria and the discovery of two new species

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Science china Life sciences 2011年第9期54卷 888-921页

作者： ZHAO Peng 1,2 , luo jing 1 , zhuang wenying 1* , LIU XingZhong 1 & WU Bing 1,2 1 State key laboratory of mycology, institute of microbiology, chinese academy of sciences, beijing 100101, china 2 Graduate University of chinese academy of sciences, beijing 100049, china 1. State key laboratory of mycology Institute of Microbiology Chinese Academy of Sciences Beijing 100101 China2. Graduate University of Chinese Academy of Sciences Beijing 100049 China

关键词： GO Erratum to RNA cell DNA

Selection of a DNA barcode for Nectriaceae from fungal whole-genomes

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Science china(Life sciences) 2012年第1期55卷 80-88页

作者： ZENG ZhaoQing ZHAO Peng luo jing zhuang wenying YU ZhiHe State key laboratory of mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Graduate University of chinese academy of sciences Beijing 100049China College of Life sciences Yangtze UniversityJingzhou 434025China

A DNA barcode is a short segment of sequence that is able to distinguish species. A barcode must ideally contain enough variation to distinguish every individual species and be easily obtained. Fungi of Nectriaceae are economically important and show high species diversity. To establish a standard DNA barcode for this group of fungi, the genomes of Neurospora crassa and 30 other filamentous fungi were compared. The expect value was treated as a criterion to recognize homologous sequences. Four candidate markers, Hsp90, AAC, CDC48, and EF3, were tested for their feasibility as barcodes in the identification of 34 well-established species belonging to 13 genera of Nectriaceae. Two hundred and fifteen sequences were analyzed. Intraand inter-specific variations and the success rate of PCR amplification and sequencing were considered as important criteria for estimation of the candidate markers. Ultimately, the partial EF3 gene met the requirements for a good DNA barcode: No overlap was found between the intra-and inter-specific pairwise distances. The smallest inter-specific distance of EF3 gene was 3.19%, while the largest intra-specific distance was 1.79%. In addition, there was a high success rate in PCR and sequencing for this gene (96.3%). CDC48 showed sufficiently high sequence variation among species, but the PCR and sequencing success rate was 84% using a single pair of primers. Although the Hsp90 and AAC genes had higher PCR and sequencing success rates (96.3% and 97.5%, respectively), overlapping occurred between the intraand inter-specific variations, which could lead to misidentification. Therefore, we propose the EF3 gene as a possible DNA barcode for the nectriaceous fungi.

关键词： barcoding gap expect value fungal genomes homologous sequence PCR and sequencing success rate sequence variation

DNA barcoding of the fungal genus Neonectria and the discovery of two new species

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Science china(Life sciences) 2011年第7期54卷 664-674页

作者： ZHAO Peng luo jing zhuang wenying LIU XingZhong WU Bing State key laboratory of mycology Institute of Microbiology Chinese Academy of Sciences Beifing 100101 China Graduate University of chinese academy of sciences Beijing 100049 China

To determine a suitable DNA barcode for the genus Neonectria,the internal transcribed spacer rDNA,β-tubulin,EF-1α,and RPB2 genes were selected as candidate markers.A total of 205 sequences from 19 species of the genus were ***-and inter-specific divergences and the ease of nucleotide sequence acquisition were treated as criteria to evaluate the feasibility of a DNA *** results indicated that any single gene among the candidate markers failed to serve as a successful barcode,while the combination of the partial EF-1α and RPB2 genes recognized all species *** tentatively propose the combined partial EF-1α and RPB2 genes as a DNA barcode for the *** this study,two cryptic species were discovered,based on the combined data of morphology and DNA barcode *** described and named these two new species *** and ***.

关键词： intra-specific variation inter-specific variation morphology DNA barcode sequence analysis

Polarization and fundamental sensitivity of 39K(133Cs)-85Rb-21Ne co-magnetometers

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chinese Physics B 2020年第4期29卷 272-283页

作者： Jian-Hua Liu Dong-Yang jing Lin zhuang Wei Quan Jiancheng Fang Wu-Ming Liu beijing National laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China School of Physical sciences University of Chinese Academy of SciencesBeijing 100190China School of Science Beijing Technology and Business UniversityBeijing 100048China School of Physics Sun Yat-Sen UniversityGuangzhou 510275China School of Instrument Science and Opto-Electronics Engineering and Science and Technology on Inertial LaboratoryBeihang UniversityBeijing 100191China

The hybrid optical pumping spin exchange relaxation free(HOPSERF)atomic co-magnetometers make ultrahigh sensitivity measurement of inertia *** wall relaxation rate has a big effect on the polarization and fundamental sensitivity for the co-magnetometer,but it is often neglected in the ***,there is almost no work about the systematic analysis of the influence factors on the polarization and the fundamental sensitivity of the HOPSERF *** we systematically study the polarization and the fundamental sensitivity of 39K-85Rb-21Ne and 133Cs-85Rb-21Ne HOPSERF co-magnetometers with low polarization limit and the wall relaxation *** 21Ne number density,the power density and wavelength of pump beam will affect the polarization greatly by affecting the pumping rate of the pump *** obtain a general formula on the fundamental sensitivity of the HOPSERF co-magnetometers due to shot-noise and the fundamental sensitivity changes with multiple systemic parameters,where the suitable number density of buffer gas and quench gas make the fundamental sensitivity *** fundamental sensitivity 7.5355×10^-11 rad·s^-1·Hz^-1/2 of 133Cs-85Rb-21Ne co-magnetometer is higher than the ultimate theoretical sensitivity 2×10^-10 rad·s^-1·Hz^-1/2 of K-21Ne co-magnetometer.

关键词： hybrid optical pumping spin exchange relaxation free co-magnetometer wall relaxation rate

Positioning taxonomic research for the future

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Zoological systematics 2022年第3期47卷 185-187页

作者： Deyuan Hong wenying zhuang Min Zhu Keping Ma Xiaoquan Wang Dawei Huang Yalin Zhang Guodong Ren Wenjun Bu Wanzhi Cai Dong Ren Ding Yang Aiping Liang Fengyan Bai Runzhi Zhang Fumin Lei Shuqiang Li Hongzhi Kong Lei Cai Yucheng Dai Chaodong Zhu Qisen Yang Jun Chen Zhongli Sha Jianping Jiang jing Che Donghui Wu Jiatang Li Qiang Wang Xinli Wei Ming Bai Xingyue Liu Xuexin Chen Gexia Qiao State key laboratory of systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China State key laboratory of mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China key laboratory of Vertebrate Evolution and Human Origins of chinese academy of sciences Institute of Vertebrate Paleontology and PaleoanthropologyChinese Academy of SciencesBeijing 100044China State key laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China College of Life sciences Nankai UniversityTianjin 300071China College of Plant Protection Northwest A&F UniversityYanglin 712100China School of Life sciences Hebei UniversityBaoding 071002China College of Plant Protection China Agricultural UniversityBeijing 10084China key Lab of Insect Evolution and Environmental Change College of Life SciencesCapital Normal UniversityBeijing 100048China School of Life sciences Tianjin Normal UniversityTianjin 300387China key laboratory of Zoological systematics and Evolution Institute of ZoologyChinese Academy of SciencesBeijing 100101China School of Ecology and Nature Conservation Beijing Forestry UniversityBeijing 10083China institute of Oceanology Chinese Academy of SciencesQingdao 266071China key laboratory of Mountain Ecological Restoration and Bioresource Utilization&Ecological Restoration and Biodiversity Conservation key laboratory of Sichuan Province Chengdu Institute of BiologyChinese Academy of SciencesChengdu 610041China State key laboratory of Genetic Resources and Evolution and Yunnan Laboratory of Molecular Biology of Domestic AnimalsKunming Institute of ZoologyChinese Academy of SciencesKunming 650223China key Lab of Wetland Ecology and Environment Northeast Institute of Geography and AgroecologyChinese Academy of SciencesChangchun 130102China key laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province Institute of Insect SciencesZhejiang UniversityHangzhou 310058

Why do taxonomists matter? The work of taxonomists is often understated if not completely misunderstood. Without taxonomists, organisms cannot be accurately identified, neither can these organisms be given universally accepted names, and reliably positioned in the phylogenetic tree of life. Thanks to the work of taxonomists over the last 269 years since Carl Linnaeus established the binomial system, we can now measure the health and wealth of our biodiversity in a refined, science-based inventory prescribed by stringent nomenclatural rules.

关键词： stated prescribed refined

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基于系统文献检索的副溶血性弧菌危害评估

毒理学杂志 2021年第6期35卷 451-454页

作者：杨庆谭壮生敬海明仝国辉北京市疾病预防控制中心/北京市预防医学研究中心食物中毒诊断溯源技术北京市重点实验室北京100013

目的对副溶血性弧菌的健康危害开展危害评估。方法本研究基于系统文献检索方法(systematic Literature Searches, SLS),对副溶血性弧菌的毒理学数据进行系统的文献检索,并根据搜集到的毒理学数据开展健康危害评估。结果致病性副溶血性... 详细信息

目的对副溶血性弧菌的健康危害开展危害评估。方法本研究基于系统文献检索方法(systematic Literature Searches, SLS),对副溶血性弧菌的毒理学数据进行系统的文献检索,并根据搜集到的毒理学数据开展健康危害评估。结果致病性副溶血性弧菌对人体有致病作用。我国现阶段采用食品安全国家标准GB 4789.7-2013>和国家标准GB 29921-2013>方法进行检测,判断水产制品副溶血性弧菌标准为:定性实验中为无检出副溶血性弧菌;在定量检测中致病菌可接受水平的限量值为100 MPN/g,最高安全限量值为1000 MPN/g(MPN/g表示每g检样中副溶血性弧菌最可能数)。结论所确定的副溶血性弧菌定性和定量检测标准值,可用于进一步食品安全风险评估。

关键词：副溶血性弧菌危害评估系统文献检索