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The aquaporin MePIP2;7 improves MeMGT9-mediated Mg2+ acquisition in cassava

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Journal of Integrative Plant Biology 2023年第10期65卷 2349-2367页

作者： Qiuxiang Ma Yancai Feng Shu luo lu Cheng Weijing Tong xinlu lu Youzhi Li Peng Zhang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/College of Life Science and Technology Guangxi UniversityNanning 530004China

Aquaporins are important transmembrane water transport proteins which transport water and several neutral molecules. However, how aquaporins are involved in the synergistic transport of Mg2+and water remains poorly understood. Here, we found that the cassava aquaporin Me PIP2;7 was involved in Mg2+transport through interaction with Me MGT9, a lower affinity magnesium transporter protein. Knockdown of Me PIP2;7 in cassava led to magnesium deficiency in basal mature leaves with chlorosis and necrotic spots on their edges and starch over-accumulation. Mg2+content was significantly decreased in leaves and roots of Me PIP2;7-RNA interference(PIP-Ri) plants grown in both field and Mg2+-free hydroponic solution. Xenopus oocyte injection analysis verified that Me PIP2;7 possessed the ability to transport water only and Me MGT9 was responsible for Mg^(2+)*** importantly, Me PIP2;7 improved the transportability of Mg^(2+)via Me MGT9 as verified using the CM66 mutant complementation assay and Xenopus oocytes expressing system. Yeast twohybrid, bimolecular fuorescence complementation,co-localization, and co-immunoprecipitation assays demonstrated the direct protein–protein interaction between Me PIP2;7 and Me MGT9 in vivo. Mg2+fux was significantly elevated in Me PIP2;7-overexpressing lines in hydroponic solution through non-invasive micro-test technique analysis. Under Mg^(2+)-free condition, the retarded growth of PIP-Ri transgenic plants could be recovered with Mg^(2+)supplementation. Taken together, our results demonstrated the synergistic effect of the Me PIP2;7 and Me MGT9 interaction in regulating water and Mg2+absorption and transport in cassava.

关键词： aquaporin cassava magnesium transporter nutrient utilization protein interaction

Lysozyme inhibits postharvest physiological deterioration of cassava

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Journal of Integrative Plant Biology 2022年第3期64卷 621-624页

作者： Xiaoyun Wu Jia Xu Qiuxiang Ma Sulaiman Ahmed xinlu lu Erjun Ling Peng Zhang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesThe Chinese Academyof ScienceShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Insect Developmental and Evolutionary Biology CAS Center for Excellence in Molecular Plant SciencesThe Chinese Academy of ScienceShanghai 200032Chin

After harvest,cassava(Manihot esculenta Crantz)storage roots undergo rapid postharvest physiological deterioration,producing blue-brown discoloration in the vasculature due to the production of polyphenolics(mainly quinonesand coumarins)by enzymes such as polyphenol oxidase(PPO).Here,we report the application of hen egg-white lysozyme(HEWL),a natural PPO inhibitor,in transgenic cassava to repress the symptoms of postharvest physiological *** HEWL-expressing transgenic plants had lower levels of the two main cassava coumarins tested,scopoletin and scopolin,compared with wild ***-expressing cassava also showed increased tolerance of oxidative ***,the lysozyme-PPO system proved to be functional in plants for repressing PPO-mediated commercial product browning.

关键词： cassava inhibitor lysozyme polyphenol oxidase post-harvest physiological deterioration

Production of very-high-amylose cassava by post-transcriptional silencing of branching enzyme genes

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Journal of Integrative Plant Biology 2020年第6期62卷 832-846页

作者： Wenzhi Zhou Shanshan Zhao Shutao He Qiuxiang Ma xinlu lu Xiaomeng Hao Hongxia Wang Jun Yang Peng Zhang National Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologyShanghai Institutes for Biological Sciencesthe Chinese Academy of SciencesShanghai 200032China Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Plant Science Research CenterShanghai Chenshan Botanical Gardenthe Chinese Academy of SciencesShanghai 201602China University of Chinese Academy of Sciences Beijing 100049China

High amylose starch can be produced by plants deficient in the function of branching enzymes(BEs).Here we report the production of transgenic cassava(Manihot esculenta Crantz)with starches containing up to 50%amylose due to the constitutive expression of hair-pin ds RNAs targeting the BE1 or BE2 *** BE1-RNAi plant lines(BE1i)and BE2-RNAi plant lines(BE2i)were grown up in the field,but with reduced total biomass *** high amylose content in the storage roots of BE2i plant lines was *** starch granules of BE1i and BE2i plants had similar morphology as wild type(WT),however,the size of BE1i starch granules were bigger than that of *** of amylograms and thermograms of all three sources of storage starches revealed dramatic changes to the pasting properties and a higher melting temperature for BE2i *** chain length distribution analysis showed a slight increase in chains of DP>36 in BE1i lines and a dramatic increase in glucan chains between DP 10-20 and DP>40 in BE2i ***,BE2i starches displayed a B-type X-ray diffraction pattern instead of the A-type pattern found in BE1i and WT ***,cassava BE1 and BE2 function differently in storage root starch biosynthesis.

关键词： chains branching pattern

Quasi-cyclic Random Projection Code and Hardware Implementation

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Communications and Network 2013年第3期5卷 86-92页

作者： Saifeng Shi Min Wang xinlu lu Jun Wu College of Electronics and Information Engineering Tongji University Shanghai China College of Electronics and Information Engineering Tongji University Shanghai China School of Mathematics and Computer Science Gannan Normal University Ganzhou China

Random Projection Code (RPC) is a mechanism that combines channel coding and modulation together and realizes rate adaptation in the receiving end. Random projection code’s mapping matrix has significant influences on decoding performance as well as hardware implementation complexity. To reduce hardware implementation complexity, we design a quasi-cyclic mapping matrix for RPC codes. Compared with other construction approaches, our design gets rid of data filter component, thus reducing chip area 7284.95 um2, and power consumption 331.46 uW in 0.13 um fabrication. Our simulation results show that our method does not cause any performance loss and even gets 0.2 dB to 0.5 dB gain at BER 10-4.

关键词： Quasi-cyclic Mapping Matrix Random Projection Code Hardware Implementation

外源褪黑素对木薯脆性胚性愈伤组织增殖的影响

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植物生理学报 2023年第10期59卷 1881-1889页

作者：程露郑偲葶陆新露张鹏马秋香中国科学院分子植物科学卓越创新中心植物分子遗传国家重点实验室上海200032 中国科学院大学北京100049

以木薯(Manihot esculenta)‘TMS60444’脆性胚性愈伤组织(FEC)为材料,研究了在GD基础培养基中添加不同浓度外源褪黑素(melatonin)对FEC增殖的影响。结果表明:在不含氨氯吡啶酸(4-氨基-3,5,6-三氯吡啶-2-羧酸)的GD培养基(GD0)中加入不... 详细信息

以木薯(Manihot esculenta)‘TMS60444’脆性胚性愈伤组织(FEC)为材料,研究了在GD基础培养基中添加不同浓度外源褪黑素(melatonin)对FEC增殖的影响。结果表明:在不含氨氯吡啶酸(4-氨基-3,5,6-三氯吡啶-2-羧酸)的GD培养基(GD0)中加入不同浓度褪黑素,其中低浓度(<100μmol·L^(-1))褪黑素可以促进FEC增殖,但高浓度褪黑素使FEC生长缓慢并褐化。在含有12 mg·L^(-1)氨氯吡啶酸的GD培养基(GD1)中添加不同浓度褪黑素(0、0.1、1、10、100μmol·L^(-1))进行培养,FEC鲜重增长率明显不同;以添加10μmol·L^(-1)褪黑素效果最佳,可使FEC保持较好的增殖状态,并显著提高木薯FEC鲜重的增长率,比不加褪黑素的GD1-1培养基中增加了2.57倍。用10μmol·L^(-1)褪黑素处理木薯FEC,其内源褪黑素代谢通路相关基因表达发生改变,抗氧化相关基因明显上调表达;并且抗氧化酶活性显著增强,抗氧化能力明显提高。本研究为木薯遗传转化中延长木薯FEC使用周期和提高增殖效率提供思路和技术。

关键词：木薯褪黑素脆性胚性愈伤增殖抗氧化

A novel Sn-based coordination polymer with high-efficiency and ultrafast lithium storage

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Journal of Materials Science & Technology 2022年第2期97卷 156-164页

作者： xinlu Zhang lu Han Junfeng Li Ting lu Jinliang Li Guang Zhu Likun Pan Shanghai Key Laboratory of Magnetic Resonance School of Physics and Electronic ScienceEast China Normal UniversityShanghai 200241China Siyuan Laboratory Guangzhou Key Laboratory of Vacuum Coating Technologies and New Energy MaterialsGuangdong Provincial Engineering Technology Research Center of Vacuum Coating Technologies and New Energy MaterialsDepartment of PhysicsJinan UniversityGuangzhouGuangdong 510632China Key Laboratory of SpinElectron and Nanomaterials of Anhui Higher Education Institutes Suzhou UniversitySuzhou 234000China

Recently,Coordination Polymers(CPs)have been widely utilized as energy storage materials for reversible Lithium-Ion Batteries(LIBs)benefiting from their tunable building blocks and adjusted electrochemical ***,the unsatisfied electrochemical behavior of CPs with poor conductivity and sluggish ion transport kinetics is still a bottle-neck for their large-scale energy storage applications in ***,we display the rational fabrication of a conductive Sn-based coordination polymer(Sn-DHTPA)via judiciously choosing suitable building *** Sn-DHTPA is employed as anode for LIBs,exhibiting superior reversible storage capacity of 1142.6 m A h g^(-1) at 0.1 A g^(-1) after 100 cycles and impressive rate storage capability of 287.7 m A h g^(-1)at 20 A g^(-1).More importantly,a robust cycling performance of 205.5 m A h g^(-1) at an extra-high current density of 20 A g^(-1) are observed without remarkable capacity-fading up to1000 *** behavior superiority of Sn-DHTPA is related to its advanced architecture with abundant lithium storage sites,high electrical conductivity and rapid lithium transport.A series of ex-situ characterizations reveal that the impressive lithium storage capacity is contributed by the redox active sites of both the aromatic linker and metal center related to in-situ generated metallic nanoparticles dispersed in the skeleton.

关键词： Sn-based coordination polymer High electrical conductivity Li-ion batteries Anode Lithium storage mechanism

Increasing Anthropogenic Mercury Pollution over the Last 200 Years Revealed by Lagoonal Sediments from Hainan Island,South China

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Chinese Geographical Science 2023年第6期33卷 1127-1140页

作者： LI Yanting XUE Jibin CHEN Jingqiang lu Yi MA xinlu School of Geographical Sciences South China Normal UniversityGuangzhou510631China

The toxic heavy metal mercury(Hg)has significantly enhanced the global Hg cycle influenced by human activities over the last *** this study,we presented a high-resolution Hg deposition history between∼1780 and 2015 AD in a sediment core from Xincun Lagoon,located in the southeastern Hainan Island,South China,and analyzed it in conjunction with geochemical elements,grain-size distribution,organic matter,and HYSPLIT backward trajectory *** objective was to investigate the influencing factors affecting historical Hg deposition in relatively remote regions and assess the extent of the effects of natural background and human *** results showed that the Hg in the sediment was deposited primarily through atmospheric deposition,which was closely related to regional and even global human *** Hg contamination increased gradually from the 1830s to 1850s,possibly due to Hg emissions from Opium Wars I and II occurring in southeastern *** broad peaks of anthropogenic Hg were observed during the 1910s to 1950s and in the 1980s,likely associated with the two world wars and modern Chinese *** addition,a further sharp increase in anthropogenic Hg from the mid-1970s to the present occurred,likely originating from the intense industrial activities in China triggered by the reform and opening-up policy of China in 1978 and some countries in Southeast Asia.

关键词： Mercury deposition pollution history anthropogenic impact Xincun Lagoon Hainan,China

1992年与2002年综合医院门诊抑郁症患者治疗情况与5年复诊率比较

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中国临床医学 2010年第3期17卷 439-441页

作者：张晶璟季建林陈志青许国勤陆新茹复旦大学附属中山医院医学心理科上海200032 上海市第一人民医院上海市第一人民医院医学心理科上海200080

目的:比较1992年与2002年全年某综合性医院医学心理专科门诊抑郁症患者的药物治疗及随访率变化。方法:分别选取1992年1月1日—12月31日在上海市第一人民医院医学心理门诊就诊的抑郁症初诊病例以及2002年1月1日—2002年12月31日就诊的抑... 详细信息

目的:比较1992年与2002年全年某综合性医院医学心理专科门诊抑郁症患者的药物治疗及随访率变化。方法:分别选取1992年1月1日—12月31日在上海市第一人民医院医学心理门诊就诊的抑郁症初诊病例以及2002年1月1日—2002年12月31日就诊的抑郁症初诊病例,记录其药物应用情况及初诊后1个月、6个月、1年、3年和5年的复诊和失随访情况。结果:初诊抑郁症患者1992年35例,2002年305例,10年间增长了约9倍。常用抗抑郁药物1992年为三环类(71.34%),2002年则为选择性5-羟色胺再摄取抑制剂(95.41%)(P<0.05)。门诊抑郁症患者坚持门诊复诊、随时间推移下降,1992年与2002年的1个月、6个月、1年、3年、5年门诊复诊率分别为42.86%和53.77%、22.86%和34.43%、22.86%和26.89%、11.43%和12.79%、5.71%和10.49%。结论:10年间门诊抑郁症患者的就诊例数明显增加,抗抑郁药物的应用种类发生了显著变化,但维持治疗、坚持门诊随访者的比例仍较低。

关键词：抑郁症门诊患者随访率

The carbon border adjustment mechanism is inefficient in addressing carbon leakage and results in unfair welfare losses

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Fundamental Research 2024年第3期4卷 660-670页

作者： xinlu Sun Zhifu Mi lu Cheng D'Maris Coffman Yu Liu The Bartlett School of Sustainable Construction University College LondonLondon WC1E 7HBUnited Kingdom College of Urban and Environmental Sciences Peking UniversityBeijing 100871China

The European Commission has proposed a Carbon Border Adjustment Mechanism(CBAM)to reduce carbon leakage and create a level playing field for its domestic products and imported ***,the effectiveness of the proposal remains unclear,especially when it triggers threats of retaliation from trading partners of the European *** apply a Computable General Equilibrium model-Global Trade Analysis Project-to assess the economic and environmental impacts of different CBAM *** we show that the effectiveness of the CBAM to address carbon leakage risks is rather limited,and the CBAM raises concerns over global welfare costs,Correct to Gross Domestic Product(GDP)losses,and violation of equality *** retaliation leads to multiplied welfare losses,which would mostly be borne by poor *** results question the carbon leakage reduction effect of a unilateral trade policy and suggest that climate change mitigation still needs to be performed within the framework of international cooperation.

关键词： Carbon border adjustment mechanism Carbon leakage Climate change mitigation Floorcarbon price Trade retaliation