检索结果-南通市图书馆

Biomedical and Environmental Sciences 2024年第4期37卷 436-439页

作者： luO Xi LI Xue ZHANG Miao Miao HUANG Qing Lian ZHANG Yi Quan JI Shen Jie lu ren fei Department of Clinical Laboratory Affiliated Nantong Hospital 3 of Nantong UniversityNantong Third People’s HospitalNantong 226006JiangsuChina Department of Clinical Laboratory Qidong People’s HospitalQidong 226200JiangsuChina

Vibrio parahaemolyticus,a gram-negative halophilic bacterium that naturally inhabits coastal waters,causes gastroenteritis,skin infections,and septicemia in human beings[1].This bacterium produces multiple virulence factors,including thermostable direct hemolysin(TDH),TDH-related hemolysin(TRH),type Ⅲ secretion system 1(T3SS1),T3SS2,type Ⅵ secretion system 1(T6SS1),and T6SS2[1].Furthermore,Ⅴ.parahaemolyticus forms biofilms on the surface,which help it in adapting to unfavorable conditions[2].Mature biofilm formation requires special structures,including lateral and polar flagella,exopolysaccharide(EPS),and type Ⅳ pili[2].

关键词： coastal system T3SS

H-NS Represses Biofilm Formation and c-di-GMP Synthesis in Vibrio parahaemolyticus

在线全文

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2022年第9期35卷 821-829页

作者： XUE Xing Fan ZHNAG Miao Miao SUN Jun Fang LI Xue WU Qi Min YIN Zhe YANG Wen Hui NI Bin HU Ling fei ZHOU Dong Sheng lu ren fei ZHANG Yi Quan School of Medicine Jiangsu UniversityZhenjiang 212013JiangsuChina Department of Clinical Laboratory Affiliated Nantong Hospital 3 of Nantong UniversityNantong 226006JiangsuChina State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China

Objective This study aimed to investigate the regulation of histone-like nucleoid structuring protein(H-NS)on biofilm formation and cyclic diguanylate(c-di-GMP)synthesis in Vibrio parahaemolyticus *** Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining,quantification of c-di-GMP,quantitative real-time polymerase chain reaction,LacZ fusion,and electrophoretic-mobility shift *** The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in *** RIMD2210633.H-NS can bind the upstream promoter-proximal DNA regions of scrA,scrG,VP0117,VPA0198,VPA1176,VP0699,and VP2979 to repress their *** genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP *** One of the mechanisms by which H-NS represses the biofilm formation by *** RIMD2210633 may be via repression of the production of intracellular c-di-GMP.

关键词： Vibrio parahaemolyticus Biofilm H-NS C-di-GMP

Multidimensional Analgesia of Acupuncture by Increasing Expression of MD2 in Central Nervous System

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2024年第11期30卷 1035-1044页

作者： LI Wan-rong ren lu-lu ZHAO Tian-tian DAI Dan-qing GAO Xiao-fei LIANG Hua-zheng XIONG Li-ze Shanghai Key Laboratory of Anesthesiology and Brain Functional Modulation Shanghai Fourth People's Hospital School of Medicine Tongji University Clinical Research Center for Anesthesiology and Perioperative Medicine Shanghai Fourth People's Hospital School of Medicine Tongji University Translational Research Institute of Brain and Brain-Like Intelligence Shanghai Fourth People's Hospital School of Medicine Tongji University Department of Anesthesiology and Perioperative Medicine Shanghai Fourth People's Hospital School of Medicine Tongji University School of Rehabilitation Southwest Jiaotong University Monash Suzhou Research Institute

Objective:To investigate changes of myeloid differentiation factor 2（MD2） in inflammationinduced pain and acupuncture-mediated ***:Mice were randomly divided into three groups by a random number table method:saline group（n=16）,complete Freund's adjuvant（CFA） group（n=24） and CFA+electroacupuncture（EA） group（n=26）.Inflammation-induced pain was modelled by injecting CFA to the plantar surface of the hind paw of mice and EA was applied to bilateral Zusanli（ST 36） to alleviate *** mice in the CFA+EA group received EA treatment（30 min/d for 2 weeks） 24 h after *** in the saline and CFA groups received sham ***-Frey test and Hargreaves test were used to assess the pain *** and spinal tissues were collected for immunofluorescence staining or Western blotting to quantify changes of MD2 ***:CFA successfully induced plantar pain and EA significantly alleviated pain 3 days after modelling（Pluorescence intensity of MD2 neurons were also significantly increased in laminae Ⅰ-Ⅱ_o（Plum,or the brainstem（P>0.05）.Fluorescence staining showed significant decrease in the level of MD2 in periagueductal gray（PAG） and locus coeruleus（LC） after CFA injection on day 7（Plusion:The unique changes of MD2 suggest that EA may exert the analgesic effect through modulating neuronal activities of the superficial laminae of the spinal cord and certai

关键词： dorsal horn electroacupuncture inflammation-induced pain locus coeruleus myeloid differentiation factor 2 periaqueductal gray

Lewis Base Catalyzed Selenofunctionalization of Alkynes with Acid-Controlled Divergent Chemoselectivity

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2024年第14期42卷 1623-1629页

作者： Ling-Ling Chen ren-fei Cao Hua Ke Zhi-Min Chen School of Chemistry and Chemical Engineering Shanghai Key Laboratory for Molecular Engineering of Chiral DrugsShanghai Jiao Tong UniversityShanghai200240 China Engineering Technology Research Center for Environmental Protection Materials Pingxiang UniversityPingxiangJiangxi337055 China

Lewis base catalyzed and Brønsted acid controlled chemodivergent electrophilic selenofunctionalizations of alkynes were developed for the first *** selenium-containing tetrasubstituted alkenes were readily obtained in moderate to excellent yields with complete E/Z *** the substrates were 1-ethynyl naphthol derivatives,linear selenium-containing tetrasubstituted alkenes were produced via intermolecular oxygen nucleophilic attack in the absence of acid additive;in contrast,cyclic selenium-containing tetrasubstituted alkenes were generated through intramolecular carbon nucleophilic capture with the addition of Brønsted acid.

关键词： Alkynes Electrophilic addition Chemoselectivity Lewis base catalysis Selenofunctionalization

Defects in phospholipase C zeta cause polyspermy and low fertilization after conventional IVF:not just ICSI failure

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2024年第2期26卷 175-182页

作者： Jian-Fang Che Hui-Xia Wu Si-Cong Zeng Yue-ren Wu Jing Dai De-Hua Cheng fei Gong Guang-Xiu lu Ge Lin Can Dai School of Medicine Hunan Normal UniversityChangsha 410013China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410008China Clinical Research Center for Reproduction and Genetics in Hunan Province Changsha 410078China National Engineering and Research Center of Human Stem Cell Changsha 410205China

Phospholipase C zeta(PLC)is a key sperm-borne oocyte-activating factor that triggers Ca^(2+)oscillations and the subsequent block to polyspermy following gamete *** in PLCZ1,the gene encoding PLCζ,cause male infertility and intracytoplasmic sperm injection(ICSI)fertilization failure;and PLCζ expression and localization patterns are significantly correlated with ICSI fertilization rate(FR).However,in conventional in vitro fertilization(cIVF),whether and how sperm PLCζ affects fertilization remain ***,we identified one previously reported and two novel PLCZ1 mutations associated with polyspermy in vitro that are characterized by excessive sperm-zona binding and a delay in pronuclei(PN)*** staining and oocyte activation testing revealed that virtually all spermatozoa from patients lacked functional PLCζ and were thus unable to evoke Ca^(2+) *** with an artificial oocyte activation treatment successfully rescued the polyspermic phenotype and resulted in a live ***,we analyzed PLCζ in an additional 58 males after cIVF treatment in the Reproductive and Genetic Hospital of CiTiC-Xiangya(Changsha,China)between February 2019 and January *** found that the proportion of spermatozoa that expressed PLCζ was positively correlated with both 2PN rate and total *** optimal cutoff value below which males were likely to experience low FR(total FR≤30%)after clVF was 56.7%for the proportion of spermatozoa expressing *** study expands the mutation and the phenotypic spectrum of PLCZ1 and further suggests that PLCζ constitutes a promising biomarker for identifying low FRs cases in cIVF due to sperm-related oocyte activation deficiency and that sperm PLCζ analysis may benefit the widermale population and not onlymen with IcsI failure.

关键词： conventional IVF gene variants PLCζ polyspermy

Effective Nucleic Acid Contamination Disinfection in Laboratory Settings using Ozone Gas

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Biomedical and Environmental Sciences 2024年第9期37卷 1095-1100页

作者： Yingyi Long Linlin Liang Xingyan Zhou fei ren lu Wang Peng Zhang Jing Wang Department of Laboratory The Second People’s Hospital of Jiulongpo DistrictChongqing 400030China Jiulongpo District Center for Disease Control and Prevention Chongqing 400030China

The Nucleic Acid Laboratory,a division of the Clinical Laboratory Department,is responsible for performing real-time PCR(qPCR)assays to rapidly detect infectious diseases,genetic disorders,and more[1].As the gold standard,qPCR has played an indispensable role in diagnosing pandemic infections,such as the coronavirus disease 2019(COVID-19)[2].

关键词： qPCR indispensable performing

Calcium-based polymers for suppression of soil acidification by improving acid-buffering capacity and inhibiting nitrification

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2024年第5期139卷 138-149页

作者： fei Kang Yunshan Meng Yanning Ge Yun Zhang Haixiang Gao Xueqin ren Jie Wang Shuwen Hu Beijing Key Laboratory of Farmland Soil Pollution Prevention-control and Remediation College of Resources and Environmental SciencesChina Agricultural UniversityBeijing 100193China Department of Applied Chemistry China Agricultural UniversityBeijing 100193China

Soil acidification is a major threat to agricultural sustainability in tropical and subtropical *** and environmentally friendly materials,such as calcium lignosulfonate(CaLS),calcium poly(aspartic acid)(PASP-Ca),and calcium polyγ-glutamic acid(γ-PGA-Ca),are known to effectively ameliorate soil ***,their effectiveness in inhibiting soil acidification has not been *** study aimed to evaluate the effect of CaLS,PASP-Ca,andγ-PGA-Ca on the resistance of soil toward acidification as directly and indirectly(i.e.,via nitrification)caused by the application of HNO_(3)and urea,*** comparison,Ca(OH)_(2)and lignin were used as the inorganic and organic controls,*** the materials,γ-PGA-Ca drove the substantial improvements in the pH buffering capacity(pHBC)of the soil and exhibited the greatest potential in inhibiting HNO_(3)-induced soil acidification via protonation of carboxyl,complexing with Al~(3+),and cation exchange *** acidification induced by urea,CaLS was the optimal one in inhibiting acidification and increasing exchangeable acidity during ***,the sharp reduction in the population sizes of ammonia-oxidizing bacteria(AOB)and ammonia-oxidizing archaea(AOA)confirmed the inhibition of nitrification via CaLS ***,compared to improving soil pHBC,CaLS may play a more important role in suppressing indirect ***,γ-PGA-Ca was superior to PASP-Ca and CaLS in enhancing the soil pHBC and the its resistance to acidification induced by HNO_(3) addition,whereas CaLS was the best at suppressing urea-driven soil acidification by inhibiting *** conclusion,these results provide a reference for inhibiting soil re-acidification in intensive agricultural systems.

关键词： Calcium-based polymer Soil acidification pH buffering capacity Nitrification inhibition

Selective CO_(2)Electroreduction to Multi-Carbon Products on Organic-Functionalized CuO Nanoparticles by Local Micro-Environment Modulation

在线全文

学校读者我要写书评

暂无评论

Nano-Micro Letters 2024年第12期16卷 120-132页

作者： Shan ren Xi Cao Qikui Fan Zhimao Yang fei Wang Xin Wang Licheng Bai Jian Yang Materials Interfaces Center Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhen 518055GuangdongPeople’s Republic of China Key Laboratory of Functional Molecular Solids Ministry of EducationCollege of Chemistry and Materials ScienceAnhui Normal UniversityWuhu 241002AnhuiPeople’s Republic of China School of Physics Xi’an Jiaotong UniversityXi’an 710049ShaanxiPeople’s Republic of China College of Materials Science and Engineering Shenzhen UniversityShenzhen 518071GuangdongPeople’s Republic of China

Surface functionalization of Cu-based catalysts has demonstrated promising potential for enhancing the electrochemical CO_(2)reduction reaction(CO_(2)RR)toward multi-carbon(C2+)products,primarily by suppressing the parasitic hydrogen evolution reaction and facilitating a localized CO_(2)/CO concentration at the *** upon this approach,we developed surface-functionalized catalysts with exceptional activity and selectivity for electrocatalytic CO_(2)RR to C_(2+)in a neutral *** CuO nanoparticles coated with hexaethynylbenzene organic molecules(HEB-CuO NPs),a remarkable C_(2+)Faradaic efficiency of nearly 90%was achieved at an unprecedented current density of 300 mA cm^(-2),and a high FE(>80%)was maintained at a wide range of current densities(100-600 mA cm^(-2))in neutral environments using a flow ***,in a membrane electrode assembly(MEA)electrolyzer,86.14%FEC2+was achieved at a partial current density of 387.6 mA cm^(-2)while maintaining continuous operation for over 50 h at a current density of 200 mA cm^(-2).In-situ spectroscopy studies and molecular dynamics simulations reveal that reducing the coverage of coordinated K⋅H2O water increased the probability of intermediate reactants(CO)interacting with the surface,thereby promoting efficient C-C coupling and enhancing the yield of C_(2+)*** advancement offers significant potential for optimizing local micro-environments for sustainable and highly efficient C_(2+)production.

关键词： CO_(2)electroreduction to C_(2+) Neutral electrolyte Organic-functionalized CuO nanoparticles Local micro-environment

Mechanical responses of underground carriageway structures due to construction of metro tunnels beneath the existing structure:A case study

在线全文

学校读者我要写书评

暂无评论

Deep Underground Science and Engineering 2024年第2期3卷 231-246页

作者： Xin Han fei Ye Xingbo Han Chao ren Jing Song Ruliang Zhao School of Highway Chang'an UniversityXi'anChina Shandong Province Transportation Planning and Design Institute JinanChina China Water Resources Beifang Investigation Design and Research Co.Ltd.TianjinChina

To understand the mechanical response pattern of the existing structure and ground due to the construction of metro tunnels underneath,the finite difference method is adopted to study the torsional deformation and stress variation of the existing structure and the effect of underground carriageway structures on the surface *** curves of the maximum differential subsidence,torsion angle,and distortion of the cross-section of the existing structure show two peaks in succession during traversing of two metro tunnels beneath *** torsion angle of the existing structure changes when the two tunnels traverse beneath it in opposite *** first traversing of the shield tunnel mainly induces the magnitude variation in torsional deformation of the existing structure,but the second traversing of the subsurface tunnel may cause a dynamic change in the magnitude and form of torsional deformation in the existing *** shielding effect can reduce the surface subsidence caused by metro tunnel excavation to a certain extent,and the development trend of subsidence becomes slower as the excavation continues.

关键词： metro tunnel numerical simulation structural deformation surface subsidence underground carriageway structure

Multi-stable straw-like carbon nanotubes for mechanical programmability at microscale

在线全文

学校读者我要写书评

暂无评论

International Journal of Smart and Nano Materials 2024年第1期15卷 95-109页

作者： Jia Liu Yong Ma Wanjie ren fei Pan Shu Guo Yuli Chen Bin Ding National Key Laboratory of Strength and Structural Integrity Institute of Solid MechanicsBeihang University(BUAA)BejingPR China Institute of Extreme Mechanics Northwestern Polytechnical UniversityXi'anShaanxiPR China Fuselage Department Shanghai Aircraft Design and Research InstituteShanghaiPR China School of Vehicle and Energy Yanshan UniversityQinhuangdaoPR China

Inspired by macroscale 3D pixel mechanical metamaterials and microscale straw-like carbon nanotube,we propose a design of multi-stable straw-like carbon nanotubes(MSCNT)via optimizing the structure of a unit to obtain multiple stable states under dis-placement loading by molecular *** unit of MSCNT is mirror-symmetrically connected two truncated graphene cones with specific apex *** switching the LJ term in AIREBO potential,we verify that the bistability of unit is co-determined by snap-through instability and microscale ***,we examine the validity of the multi-stability of the unit cells arranged in series and in *** results indicate that the MSCNT can achieve mechanical programmability in microscale,which triggers many potential applications in need of customizing nanos-cale mechanical behaviors.

关键词： Multi-stable state straw-shaped carbon nanotube microscale adhesions mechanical programmability