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GTF2H4 regulates partial EndMT via NF-κB activation through NCOA3 phosphorylation in ischemic diseases

The Innovation 2024年第2期5卷 27-41页

作者： Zheyan Fang Gang Zhao Shuang Zhao Xueting Yu Runyang Feng You-en Zhang Haomin Li Lei Huang Zhenyang Guo Zhentao Zhang Mukaddas Abdurahman Hangnan Hong Peng Li Bing Wu Jinhang Zhu Xin Zhong Dong Huang Hao lu Xin Zhao Zhaoyang Chen Wenbin Zhang Junjie Guo Hongchao Zheng Yue He Shengying Qin haojie lu Yun Zhao Xiangdong Wang Junbo Ge Hua Li Department of Cardiology Zhongshan HospitalFudan UniversityShanghai Institute of Cardiovascular DiseasesShanghai 200032China Department of Cardiology Zhongshan HospitalFudan UniversityShanghai 200032China Department of Medical Examination Shanghai Xuhui District Central HospitalShanghai 200031China Department of Cardiology and Institute of Clinical Medicine Renmin HospitalHubei University of MedicineShiyan 442000China Clinical Data Center Children’s HospitalZhejiang University School of MedicineNational Clinical Research Center for Child HealthHangzhou 310052China Department of Molecular Celland Cancer BiologyUniversity of Massachusetts Chan Medical SchoolWorcesterMA 01605USA Bio-X Institute Key Laboratory for The Genetics of Developmental and Neuropsychiatric DisordersShanghai Jiao Tong UniversityShanghai 200030China Department of Cardiology Heart Center of Fujian ProvinceFujian Medical University Union HospitalFuzhou 350001China Department of Cardiology Sir Run Run Shaw Hospitalaffiliated with Zhejiang University School of MedicineHangzhou 310020China Department of Cardiology The Affiliated Hospital of Qingdao UniversityQingdao 266003China Department of Cardiology Shanghai Xuhui District Central HospitalShanghai 200031China Department of Cardiology Shanghai Eighth People’s HospitalShanghai 200235China Department of Chemistry and Institutes of Biomedical Sciences Fudan UniversityShanghai 200032China State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China School of Life Science and Technology Shanghai Tech University100 Haike RoadShanghai 201210China Key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China Department of Pulmonary and Critical Care Medicine Zhongshan Hospital

Partial endothelial-to-mesenchymal transition(EndMT)is an intermediate phenotype observed in endothelial cells(ECs)undergoing a transition toward a mesenchymal state to support neovascularization during(patho)physiological ***,we investigated the occurrence of partial EndMT in ECs under hypoxic/ischemic conditions and identified general transcription factor IIH subunit 4(GTF2H4)as a positive regulator of this *** addition,we discovered that GTF2H4 collaborates with its target protein excision repair cross-complementation group 3(ERCC3)to co-regulate partial ***,by using phosphorylation proteomics and site-directed mutagenesis,we demonstrated that GTF2H4 was involved in the phosphorylation of receptor coactivator 3(NCOA3)at serine 1330,which promoted the interaction between NCOA3 and p65,resulting in the transcriptional activation of NF-κB and the NF-kB/Snail signaling axis during partial *** vivo experiments confirmed that GTF2H4 significantly promoted partial EndMT and angiogenesis after ischemic ***,our findings reveal that targeting GTF2H4 is promising for tissue repair and offers potential opportunities for treating hypoxic/ischemic diseases.

关键词： ischemic activation GTF

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超大直径盾构刀盘驱动密封系统全寿命周期管理探究

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建筑机械化 2024年第1期45卷 58-62页

作者：陆豪杰中铁隧道股份有限公司河南郑州1450000

结合诸多超大直径盾构刀盘驱动密封失效案例,总结了驱动密封系统全寿命周期管理技术措施,为刀盘驱动密封系统理论研究、设计制造、应用与维护等方面积累了经验,供大家参考。

关键词：盾构刀盘超大直径驱动密封系统全寿命周期管理

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Advancements in mass spectrometry-based glycoproteomics and glycomics

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National Science Review 2016年第3期3卷 345-364页

作者： haojie lu Ying Zhang Pengyuan Yang Department of Systems Biology for Medicine School of Basic Medicine and Institutes of Biomedical SciencesFudan University Key Lab of Glycoconjugate of Ministry of Health and Birth Control Fudan University Department of Chemistry Fudan University

Protein N-glycosylation plays a crucial role in a considerable number of important biological *** studies on glycoproteomes and glycomes have already characterized many glycoproteins and glycans associated with cell development, life cycle, and disease progression. Mass spectrometry(MS) is the most powerful tool for identifying biomolecules including glycoproteins and glycans, however, utilizing MS-based approaches to identify glycoproteomes and glycomes is challenging due to the technical difficulties associated with glycosylation analysis. In this review, we summarize the most recent developments in MS-based glycoproteomics and glycomics, including a discussion on the development of analytical methodologies and strategies used to explore the glycoproteome and glycome, as well as noteworthy biological discoveries made in glycoproteome and glycome research. This review places special emphasis on China, where scientists have made sizeable contributions to the literature, as advancements in glycoproteomics and glycomincs are occurring quite rapidly.

关键词： mass spectrometry glycoproteomics glycomics

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Fractionation-free negative enriching for in-depth C-terminome analysis

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Chinese Chemical Letters 2022年第3期33卷 1343-1345页

作者： Jingtian lu Ting Wang Huimin Bao haojie lu Department of Chemistry and Shanghai Cancer Center Fudan UniversityShanghai 200438China Institutes of Biomedical Sciences and NHC Key Laboratory of Glycoconjugates Research Fudan UniversityShanghai 200032China

Herein, we developed a fractionation-free negative enriching method incorporating methylamidation, siteselective dimethylation and aldehyde resin coupling(MADMAR) for in-depth C-terminome analysis. The methylamidation blocked the free carboxyl group on proteins first, followed by Lys C digestion of methylamidated proteins. Then, the site-selective dimethylation blocked the N-terminal amino group of the digested peptides without affecting the amino groups of lysine. Finally, the aldehyde resin was used to capture non-C-terminal peptides containing amino groups from lysine, while leaving the C-terminal peptides without free amino group in the supernatant for its analysis. We identified 1359 database-annotated protein C-termini from 50 μg He La proteins, which was 74% more than our previous method based on aldehyde resin. Moreover, 279 protein neo-C-termini were identified.

关键词： C-terminome In-depth analysis Negative enrichment Neo-C-termini Methylamidation HeLa

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Comprehensive Profiling for Histone H4 of Human Liver Cells Using High Resolution LTQ-Orbitrap Mass Spectrometry

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Chinese Journal of Chemistry 2011年第1期29卷 171-177页

作者： Nie, Aiying lu, haojie Yang, Pengyuan He, Fuchu Department of Chemistry and Institute of Biomedical Sciences Fudan University Shanghai 200433 China State Key Laboratory ofProteomics Beijing Proteome Research Center Beijing Institute of Radiation Medicine Beijing 102206 China

The post translational modifications of histone variants are playing an important role in the structure of chromatin, the regulation of gene activities and the diagnosis of diseases, and conducting in-depth researches and discovering new sites depend on new and rational analytical methods to some extent. In this work, the combinatorial method of high resolution LTQ-Orbitrap mass spectrometry and multiple enzymes was employed to identify the post translational modifications （PTMs） of histone H4 of human liver cells. The novel methylation site, argnine 67 （R 67）, was observed besides some sites reported previously such as lysine 31 （K 31）, lysine 44 （K 44）, argnine 55 （R 55） and lysine 59 （K 59） in the global domain. Meanwhile, various combinations of acetylation of lysine 5 （K 5）, lysine 8 （K 8）, lysine 12 （K 12）, lysine 16 （K 16） and methylation of lysine 20 （K 20） in the NH2-terminal tails were also identified after the LC-MS/MS analysis of trypsin, Arg-C, Glu-C and chymotrypsin digests.

关键词： histone post translational modification mass spectrometry enzymes

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Discover the Post-Translational Modification Proteome Using Mass Spectrometry

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Chinese Journal of Chemistry 2021年第3期39卷 550-558页

作者： Ying Zhang Caiyun Fang Huimin Bao Wenjuan Yuan haojie lu Shanghai Cancer Center and Institutes of Biomedical Sciences Fudon UniversityShanghai 200032China Department of Chemistry and NHC Key Laboratory of Glycoconjugates Research Fudan UniversityShanghai 200032China

Protein post-translational modifications(PTMs)are chemical modifications on *** play a key role in many cellular processes by influencing the structure of proteins and dynamically regulating their ***,characterizing PTMs at proteome level is critical to provide invaluable insight into the functions of proteins underlying different biological *** in modern proteomics technologies including sample preparation,chromatography separation as well as mass spectrometry have propelled the PTMs proteome to further *** the past decade,to better examine the PTMs with high sensitivity and selectivity,our group have developed a series of MS-based novel analytical approaches to study the protein ***,we mainly introduce these approaches developed by our group and discuss how to overcome the technical obstacles of studying various protein PTMs with mass spectrometry.

关键词： Proteome Mass spectrometry Post translational modification Qualitative analysis Quantitative analysis

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High-throughput site-specific N-glycoproteomics reveals glyco-signatures for liver disease diagnosis

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National Science Review 2023年第1期10卷 214-225页

作者： Zhenyu Sun Bin Fu Guoli Wang Lei Zhang Ruofan Xu Ying Zhang haojie lu Shanghai Cancer Center and Institutes of Biomedical Sciences Fudan University Department of Chemistry and NHC Key Laboratory of Glycoconjugates Research Fudan University Eleanor Roosevelt College University of California San Diego

The glycoproteome has emerged as a prominent target for screening biomarkers,as altered glycosylation is a hallmark of cancer *** this work,we incorporated tandem mass tag labeling into quantitative glycoproteomics by developing a chemical labeling-assisted complementary dissociation method for the multiplexed analysis of intact *** from the complementary nature of two different mass spectrometry dissociation methods for identification and multiplex labeling for quantification of intact N-glycopeptides,we conducted the most comprehensive site-specific and subclass-specific N-glycosylation profiling of human serum immunoglobulin G(IgG) to *** analysing the serum of 90 human patients with varying severities of liver diseases,as well as healthy controls,we identified that the combination of IgG1-H3N5F1 and IgG4-H4N3 can be used for distinguishing between different stages of liver ***,we used targeted parallel reaction monitoring to successfully validate the expression changes of glycosylation in liver diseases in a different sample cohort that included 45 serum samples.

关键词： glycoproteomics high-throughput quantification N-glycopeptides liver disease

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Ultra-sensitive and wide applicable strain sensor enabled by carbon nanofibers with dual alignment for human machine interfaces

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Nano Research 2023年第3期16卷 4093-4099页

作者： Peng Bi Mingchao Zhang Shuo Li haojie lu Haomin Wang Xiaoping Liang Huarun Liang Yingying Zhang Key Laboratory of Organic Optoelectronics and Molecular Engineering of the Ministry of Education Department of ChemistryTsinghua UniversityBeijing 100084China Laboratory of Flexible Electronics Technology Tsinghua UniversityBeijing 100084China

Flexible strain sensors with high sensitivity,wide detection range,and low detection limit have continuously attracted great interest due to their tremendous application potential in areas such as health/medical-care,human-machine interface,as well as safety and *** both of a high sensitivity and a wide working range are desired key parameters for a strain sensor,they are usually contrary to each other to be achieved on the same sensor due to the tightly structure dependence of both of ***,a flexible strain sensor with both high sensitivity and wide strain detection range is prepared based on the design of an integrated membrane containing both of parallel aligned and randomly aligned carbon nanofibers(CNFs).The parallel aligned CNF membrane(p-CNF)exhibits a low strain detection limit and high sensitivity,while the random aligned CNF membrane(r-CNF)exhibits a large strain detection *** the advantages of both p-CNF and r-CNF,the strain sensor with stacked p-CNF and r-CNF(p/r-CNF)exhibits both high sensitivity and wide working *** gauge factor(GF)is 1,272 for strains under 0.5%and 2,266 for strain from 70%to 100%.At the same time,it can work in a wide strain range of 0.005%to 100%,fulfilling the requirements for accurately detecting full-range human *** demonstrated its applications in the recognition of facial expressions and joint ***,we constructed an intelligent lip-language recognition system,which can accurately track phonetic symbols and may help people with language disabilities,proving the potential of this strain sensor in health management and medical ***,we foresee that the dual-alignment structure design of the p/r-CNF strain sensor may also be applied in the design of other high performance sensors.

关键词： nanofiber membrane carbon nanofibers flexible sensor dual-alignment high sensitivity

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一株克氏原螯虾病原——霍乱弧菌的分离鉴定

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安徽农业大学学报 2024年第3期51卷 441-449页

作者：鹿豪洁李凯吕锦贤王永杰杨明树上海海洋大学食品学院上海201306 农业农村部水产品质量安全贮藏保鲜风险评估实验室(上海) 上海201306 海南热带海洋学院食品科学与工程学院三亚572022

从尾扇边缘溃烂的克氏原螯虾中分离到一株优势菌LK-18,并对该菌株进行种属鉴定和致病性分析。通过菌落形态观察、16S rRNA测序、血清型分析及基因特性鉴定对细菌的类别进行初步判断,并采用浸泡感染、肌肉注射和腹腔注射等方式进行人工... 详细信息

从尾扇边缘溃烂的克氏原螯虾中分离到一株优势菌LK-18,并对该菌株进行种属鉴定和致病性分析。通过菌落形态观察、16S rRNA测序、血清型分析及基因特性鉴定对细菌的类别进行初步判断,并采用浸泡感染、肌肉注射和腹腔注射等方式进行人工感染实验对细菌的致病性进行评估。经16S rRNA测序分析和4个管家基因(atp A、pyr H、rec A、gyr B)串联分析,该菌株最终被鉴定并命名为VibriocholeraeLK-18。V. choleraeLK-18经PCR鉴定不属于O1和O139血清型,但含hly毒力基因和几丁质分解相关的几丁质酶(chitinase,Chi)基因。人工感染实验虽未能重现烂尾症状,但从克氏原螯虾烂尾组织中分离的V. choleraeLK-18具有致病性,能够引起克氏原螯虾的死亡。研究结果表明霍乱弧菌LK-18是一种致病菌,不仅扩大了非O1/O139群霍乱弧菌的感染宿主范围,同时预警霍乱弧菌有导致克氏原螯虾养殖业暴发疾病的可能,因此要提前做好该疾病的预防工作,从而避免霍乱弧菌感染带来的损失。

关键词：克氏原螯虾致病菌霍乱弧菌(Vibrio cholerae) 分离鉴定

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Recent developments of nanoparticle-based enrichment methods for mass spectrometric analysis in proteomics

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Science China Chemistry 2010年第4期53卷 695-703页

作者： ZHANG LiJuan, lu haojie & YANG PengYuan Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China Department of Chemistry and Institutes of Biomedical Sciences Fudan University Shanghai China

In proteome research, rapid and effective separation strategies are essential for successful protein identification due to the broad dynamic range of proteins in biological samples. Some important proteins are often expressed in ultra low abundance, thus making the pre-concentration procedure before mass spectrometric analysis prerequisite. The main purpose of enrichment is to isolate target molecules from complex mixtures to reduce sample complexity and facilitate the subsequent analyzing steps. The introduction of nanoparticles into this field has accelerated the development of enrichment methods. In this review, we mainly focus on recent developments of using different nanomaterials for pre-concentration of low-abundance peptides/ proteins, including those containing post-translational modifications, such as phosphorylation and glycosylation, prior to mass spectrometric analysis.

关键词： enrichment nanoparticles mass spectrometry proteomics phosphorylation glycosylation

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