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Study of the pressure transient behavior of directional wells considering the effect of non-uniform flux distribution

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Petroleum Science 2024年第3期21卷 1765-1779页

作者： Yan-zhong liang Bai-lu Teng Wan-Jing luo School of Energy Resources China University of Geosciences(Beijing)Beijing100083China

During the production,the fluid in the vicinity of the directional well enters the wellbore with different rates,leading to non-uniform flux distribution along the directional ***,in all existing studies,it is oversimplified to a uniform flux distribution,which can result in inaccurate results for field ***,this paper proposes a semi-analytical model of a directional well based on the assumption of non-uniform flux ***,the direction well is discretized into a carefully chosen series of linear sources,such that the complex well trajectory can be captured and the nonuniform flux distribution along the wellbore can be considered to model the three-dimensional flow *** using the finite difference method,we can obtain the numerical solutions of the transient flow within the *** the aid of Green's function method,we can obtain the analytical solutions of the transient flow from the matrix to the *** complete flow behavior of a directional well is perfectly represented by coupling the above two types of transient ***,on the basis of the proposed model,we conduct a comprehensive analysis of the pressure transient behavior of a directional *** computation results show that the flux variation along the direction well has a significant effect on pressure *** addition,the directional well in an infinite reservoir may exhibit the following flow regimes:wellbore afterflow,transition flow,inclined radial flow,elliptical flow,horizontal linear flow,and horizontal radial *** horizontal linear flow can be observed only if the formation thickness is much smaller than the well ***,a dip region that appears on the pressure derivative curve indicates the three-dimensional flow behavior near the wellbore.

关键词： Directional well Pressure transient behavior Semi-analytical model Non-uniform flux

Numerical Simulation Study of Goaf Methane Drainage and Spontaneous Combustion Coupling

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Journal of China University of Mining and Technology 2007年第4期17卷 503-507页

作者： LI Zong-xiang lu zhong-liang WU Qiang ZHANG Ai-ran College of Vocational Liaoning Technical University Fuxin Liaoning 123000 China School of Safety Science and Engineering Henan Polytechnic University Jiaozuo Henan 454003 China Heilongjiang Institute of Science and Technology Harbin Heilongjiang 123000 China School of Energy and Safety Engineering China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China

In order to study the coupling problem between methane drainage and spontaneous combustion of residual coal in the collapsed zone after mining ignitable coal seams with high methane,we have analyzed the effects of differ-ent methane drainage modes on spontaneous combustion of residual coal through numerical simulation. The results show that deep and large flux methane drainage modes increases the air leakage from work faces to the goaf and formed new spontaneous combustion zones induced by drainage near vents,which increases the risk of self-ignition of coal—reducing the self-ignition period and enlarging the scale of self-ignition. The spontaneous upstream combustion oxidation of the main fire zone can be suppressed when both drainage and nitrogen injection were adopted. Our research results provide an effective technical measure and theoretical basis to determine the best methane drainage scheme and drainage parameters.

关键词： goal methane drainage spontaneous combustion multiphase gas flow field finite element

Alterations of FHIT Gene and P16 Gene in Nickel Transformed Human Bronchial Epithelial Cells

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Biomedical and Environmental Sciences 2006年第4期19卷 277-284页

作者： WEI-DONG JI JIA-KUN CHEN JIA-CHUN lu zhong-liang WU FEI YI SU-MEI FENG Institute for Chemical Carcinogenesis Guangzhou Medical College Guangzhou 510182 Guangdong China

Objective To study the alterations of FHIT gene and P16 gene in malignant transformed human bronchial epithelial cells induced by crystalline nickel sulfide using an immortal human bronchial epithelial cell line, and to explore the molecular mechanism of nickel carcinogenesis. Methods 16HBE cells were treated 6 times with different concentrations of NiS in vitro, and the degree of malignant transformation was determined by assaying the anchorage-independent growth and tumorigenicity. Malignant transformed cells and tumorigenic cells were examined for alterations of FHIT gene and P16 gene using RT-PCR, DNA sequencing, silver staining PCR-SSCP and Western blotting. Results NiS-treated cells exhibited overlapping growth. Compared wkh that of negative control cells, soft agar colony formation efficiency of NiS-treated cells showed significant increases （P〈0.01） and dose-dependent effects. NiS-treated cells could form tumors in nude mice, and a squamous cell carcinoma was confirmed by histopathological examination. No mutation of exon 2 and exons 2-3, no abnormal expression in pl6 gene and mutation of FHIT exons 5-8 and exons 1-4 or exons 5-9 were observed in transformed cells and tumorigenic cells. However, aberrant transcripts or loss of expression of the FHIT gene and Fhit protein was observed in transformed cells and tumorigenic cells. One of the aberrant transcripts in the FHIT gene was confirmed to have a deletion of exon 6, exon 7, exon 8, and an insertion of a 36 bp sequence replacing exon 6-8. Conclusions The FHIT gene rather than the P16 gene, plays a definite role in nickel carcinogenesis. Alterations of the FHIT gene induced by crystalline NiS may be a molecular event associated with carcinogen, chromosome fragile site instability and cell malignant transformation. FHIT may be an important target gene activated by nickel and other exotic carcinogens.

关键词： Crystalline nickel sulfide Human bronchial epithelial cell line Malignant transformation P16 gene FHIT gene

Progressive meristem and single-cell transcriptomes reveal the regulatory mechanisms underlying maize inflorescence development and sex differentiation

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Molecular Plant 2024年第7期17卷 1019-1037页

作者： Yonghao Sun liang Dong lu Kang Wanshun zhong David Jackson Fang Yang National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070China Hubei Hongshan Laboratory Wuhan 430070China Cold Spring Harbor Laboratory Cold Spring HarborNY 11724USA School of Agriculture Sun Yat-Sen UniversityShenzhen 518107China

Maize develops separate ear and tassel inflorescences with initially similar morphology but ultimately different architecture and *** detailed regulatory mechanisms underlying these changes still remain largely *** this study,through analyzing the time-course meristem transcriptomes and floret single-cell transcriptomes of ear and tassel,we revealed the regulatory dynamics and pathways underlying inflorescence development and sex *** identified 16 diverse gene clusters with differential spatiotemporal expression patterns and revealed biased regulation of redox,programmed cell death,and hormone signals during meristem differentiation between ear and ***,based on their dynamic expression patterns,we revealed the roles of two RNA-binding proteins in regulating inflorescence meristem activity and axillary meristem ***,using the transcriptional profiles of 53910 single cells,we uncovered the cellular heterogeneity between ear and tassel *** found that multiple signals associatedwith either enhancedcell death or reduced growth are responsiblefortassel pistil suppression,while part of the gibberellic acid signal may act non-cell-autonomously to regulate ear stamen arrest during sex *** further showed that the pistil-protection gene SILKLESS 1(SK1)functions antagonistically to the known pistil-suppression genes through regulating common molecular pathways,and constructed a regulatory network for pistil-fate ***,our study provides a deep understanding of the regulatory mechanisms underlying inflorescence development and sex differentiation in maize,laying the foundation for identifying new regulators and pathways for maize hybrid breeding and improvement.

关键词： maize inflorescence floral organ sex differentiation single-cell RNA sequencing RNA-binding protein

Experimental study and numerical simulation of gas/particle flow in tunneling of mine

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Journal of Coal Science & Engineering(China) 2009年第1期15卷 85-87页

作者： lu zhong-liang LI Hai-xia GUO Pei-hong JING Guo-xun School of Safety Science and Engineering Henan Polytechnic University Jiaozuo 454000 China School of Mechanical and Power Engineering Henan Polytechnic University Jiaozuo 454000 China

Respiration particles can be collected into a dust catcher by an inside inhalingand outside pressing particle collector. The work environment of the grab operator in tunneling mining was improved when a dust catcher is placed before the working face of thegrab operator. The particle movement was affected by the gas flow. The flow field insideand outside the dust collector was simulated. The effect of the operating parameter wasanalyzed. The numerical results show a good approach to predict the gas flow and particledistribution in the inside and outside of the particle collector.

关键词： grab working face particle movement inside inhaling and outside pressing particle collector

Xiuhua Mountain Museum

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Women of China 1999年第11期 32-35页

作者： zhong lu WEI liang

XIUHUA Mountain Museum,a building nestled amongthe hills,is the first private museum of the *** name is an amalgamation of the names ofthe couple who run it,Gong Daoxiu and her husband *** to Chen,the reason that he p... 详细信息

关键词： Xiuhua Mountain Museum

Analysis of photocatalytic degradation of polyamide microplastics in metal salt solution by high resolution mass spectrometry

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Journal of Environmental Sciences 2024年第5期139卷 473-482页

作者： Yunjin zhong Lebing Zhuo Wangyang lu National Engineering Lab for Textile Fiber Materials&Processing Technology(Zhejiang) School of Materials Science&EngineeringZhejiang Sci-Tech UniversityHangzhou 310018China

Microplastic pollution has become one of the most concerned focuses in the *** many treatment methods,photocatalysis is considered to be one of the most environmentally friendly *** this work,the photodegradation behavior of polyamide microplastics is studied by using polyamide 6(PA6)as model microplastics and FeCl_(3) as *** is hoped that the PA6 fiber can be effectively degraded by utilizing the strong oxidizing active species that can be produced after FeCl_(3) is irradiated in *** results shows that PA6 fiber can be almost completely degraded after 10 days of irradiation in FeCl_(3) aqueous solution,indicating that it is promising to use this new method to solve the problem of PA6type *** addition,the chain scission mechanism and degradation process of PA6 are analyzed in detail by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS),which provides a new insight for the study of polymer degradation mechanism.

关键词： FeCl_(3) Photocatalysis Polyamide 6 Chain scission

A Dielectric Ink Combining Acrylate and Cyanate Moieties for Inkjet 3D Printing with Good Thermal Stability

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Chinese Journal of Polymer Science 2024年第6期42卷 766-774页

作者： Yi-Xuan Li liang-Ying Wu Yan Yang Yao-zhong lu zhong-Ying Ji Yu-Xiong Guo Xiao-Long Wang Qiao-Sheng Pu State Key Laboratory of Applied Organic Chemistry College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhou730000China State Key Laboratory of Solid lubrication Lanzhou Institute of Chemical PhysicsLanzhou730000China Lanzhou Traditional Chinese Medicine Hospital of Orthopedics and Traumatology Lanzhou730000China Shandong Laboratory of Advanced Materials and Green Manufacturing at Yantai Yantai Zhongke Research Institute of Advanced Materials and Green Chemical EngineeringYantai264006China

Inkjet 3D printing has potential in the additive manufacturing of electronic circuits and ***,inks that can be used for printing layers with T5%>300℃ or hardness>200 MPa have been rarely *** ester(CE)polymers have excellent thermal stability,high strength,and low shrinkage compared to other common dielectric inks for inkjet 3D printing,but cannot be quickly shaped by ultraviolet(UV)irradiation or thermal *** CEs with UV-curable monomers may be a possible way to accelerate crosslinking,but there are challenges from the adverse effects of the dilution of both *** this study,dielectric inks with acrylate and cyanate moieties were *** low viscosity and surface tension of the CE precursor(Bisphenol E cyanate ester)were combined with photocurable acrylate diluent monomers and cross-linker to realize an ink suitable for inkjet 3D *** internal dual three-dimensional cross-linked network structure resin was prepared by a combination of photocuring and thermal curing with T5%up to 326.69℃,hardness up to 431.84 MPa,dielectric constant of 2.70 at 8 GHz,and shrinkage of 1.64%.The developed dielectric inks can be applied to the 3D printing of printed circuit boards and other electronic devices that require dielectric properties.

关键词： Inkjet printing Cyanate ester resin 3D doubly cross-linked network Good thermal stability

Geometrical transition properties of vortex cavitation and associated flow-choking characteristics in poppet valves

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2024年第6期25卷 455-469页

作者： liang lu zhongdong liang Yuming LIU Zhipeng WANG Shohei RYU School of Mechanical Engineering Tongji UniversityShanghai 201804China Frontiers Science Center for Intelligent Autonomous Systems Tongji UniversityShanghai 201210China Technical Research Laboratory Hitachi Construction Machinery Co.Ltd.Tsuchiura 300-0013Japan

Poppet valves have become increasingly significant in ensuring precise digital flow rate and pressure control in hydraulic systems,necessitating a more profound understanding of the geometrical properties of cavitation in them,as well as associated flow-choking *** a comparative analysis with experimentally observed cavity images,we found that large eddy simulation(LES)turbulence modeling effectively replicates the geometrical properties of cavitation in these *** analysis demonstrated that cavitation is generated from vortices that result from the interaction between the notch contracta flow and the surrounding fluid *** in the internal or external vena contracta conditions result in fixed or discrete cavities,and the length-to-diameter ratio serves as a measure of the transition between internal and external vena contracta flow *** study establishes a threshold length-to-diameter ratio of approximately 2 for the tested poppet *** specifically,in notch structures with a smaller valve opening,longer sealing length,and smaller throttling angle(corresponding to a larger length-to-diameter ratio),the liquid-to-vapor transfer process is more evident than that in the reverse direction.A long-standing vapor cavity becomes fixed inside the notch,leading to a more pronounced flow-choking *** contrast,for structures with a smaller length-to-diameter ratio,the cavitation process for discrete vapor cavities is more complete,ensuring fluid flow continuity and significantly reducing the occurrence of the flow-choking phenomenon.

关键词： Poppet valves Vena contracta Vortex flow Vapor cavity Flow-choking