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Profiling and identification of aqueous extract of Cordyceps sinensis by ultra-high performance liquid chromatography tandem quadrupole-orbitrap mass spectrometry

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Chinese Journal of Natural Medicines 2019年第8期17卷 631-640页

作者： YAO Chang-Liang QIAN Zheng-Ming TIAN Wen-Shuai XU Xiao-Qian YAN Yu SHEN Yao lu song-mao LI Wen-Jia GUO De-An R&D Department GenChim Testing (Shanghai) Co. Ltd. Shanghai 200131 China Key Laboratory of State Administration of Traditional Chinese Medicine Sunshine Lake Pharma Co. Ltd. Guangdong 523850 China Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Laboratory for TCM Standardization Technology Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 China

Characterization of aqueous extract in traditional Chinese medicine(TCM) is challenging due to the poor retention of the analytes on conventional C18 columns. This study presents a systematic characterization method based on a rapid chromatographic separation(8 min) on a polar-modified C18(Waters Cortecs T3) column of aqueous extract of Cordyceps sinensis. UHPLC-HRMS method was used to profile components in both untargeted and targeted manners by full MS/PIL/dd-MS2 acquisition approach. The components were identified or tentatively identified by reference standards comparison, fragmentation rules elucidation and available databases search. A total of 91 components, including 10 nucleobases, 20 nucleosides, 39 dipeptides, 18 amino acids and derivatives and 4 other components, were characterized from the aqueous extract of C. sinensis. And this was the first time to systematically report the presence of nucleosides and dipeptides in C. sinensis, especially for modified nucleosides. The chemical basis inquiry of this work would be beneficial to mechanism exploration and quality control of C. sinensis and related products. Meanwhile, this work also provided an effective solution for characterization of aqueous extract in TCM.

关键词： Aqueous extract Cordyceps sinensis Dipeptides Nucleosides

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蔬菜中氟吡甲禾灵残留物的测试方法

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农药 2014年第10期53卷 751-752,758页

作者：陆松茂上海市农药研究所分析中心上海200032

[目的]氟吡甲禾灵作为高毒高残农药的取代品种而在蔬菜等作物上大量使用,会加大残留量超标的风险,建立氟吡甲禾灵的残留测试方法显得尤为重要。[方法]氟吡甲禾灵经乙腈提取,固相萃取柱净化,通过配置电子捕获检测器的气相色谱仪进行检测... 详细信息

[目的]氟吡甲禾灵作为高毒高残农药的取代品种而在蔬菜等作物上大量使用,会加大残留量超标的风险,建立氟吡甲禾灵的残留测试方法显得尤为重要。[方法]氟吡甲禾灵经乙腈提取,固相萃取柱净化,通过配置电子捕获检测器的气相色谱仪进行检测。[结果]方法的最低检出质量分数为5×10-3mg/kg,回收率为70%～120%。[结论]方法的回收率、精密度等符合我国农产品安全检测标准的要求,同时其操作简便,易于推广。

关键词：氟吡甲禾灵蔬菜残留测定

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“美色”探秘的旅程

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合肥学院学报（社会科学版） 2004年第3期21卷 36-39页

作者：鲁茂松安徽省新闻出版局安徽合肥230061

20多年前 ,鲁晨光先生对语言称呼和颜色视觉的一致性提出质疑 ,指出色觉差异或颠倒的可能性 ,且并不影响称呼和交流。这有悖于人们的惯常思维引起了论争 ,由此开始了作者与鲁先生在空间时分时合 ,跨国越洋 ;在时间上断续的、跨世纪的探... 详细信息

20多年前 ,鲁晨光先生对语言称呼和颜色视觉的一致性提出质疑 ,指出色觉差异或颠倒的可能性 ,且并不影响称呼和交流。这有悖于人们的惯常思维引起了论争 ,由此开始了作者与鲁先生在空间时分时合 ,跨国越洋 ;在时间上断续的、跨世纪的探索。从《潜科学》“是否存在标准色觉” ;《自然信息》“试析达尔文留下的香甜美难题” ;《学术界》“论第二物性感知差异”等 ,直到今天 ,终于形成了哲学的两亩地———新著《色觉奥妙和哲学基本问题》和《美感奥妙和需求进化》。“美色”探秘的旅程 ,正是这哲学两亩地栽培浇灌。

关键词：色觉差异审美哲学美感进化

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电视腹腔镜胆囊切除术及小切口开腹胆囊切除术的临床比较

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现代预防医学 2011年第2期38卷 380-381页

作者：卢茂松刘强黄征焘贵州遵义医学院第三附属医院

[目的]比较分析电视腹腔镜胆囊切除术及小切口开腹胆囊切除术的临床效果。[方法]将我院2008年7月～2010年5月进行电视腹腔镜胆囊切除术的患者45例作为观察组,同期进行小切口开腹胆囊切除术的患者40例作为对照组,比较两组患者的术中、术... 详细信息

[目的]比较分析电视腹腔镜胆囊切除术及小切口开腹胆囊切除术的临床效果。[方法]将我院2008年7月～2010年5月进行电视腹腔镜胆囊切除术的患者45例作为观察组,同期进行小切口开腹胆囊切除术的患者40例作为对照组,比较两组患者的术中、术后恢复以及并发症发生情况。[结果]观察组的术中出血量为(60.5±5.0)ml,均明显少于对照组的(75.0±6.5)ml,组间差异有统计学意义(P﹤0.05);观察组的术后肠功能恢复时间、下床活动时间及住院时间分别为(15.5±3.0)min、(17.5±8.2)ml、(5.0±1.5)min,均明显短于对照组的(34.2±7.5)min、(38.0±4.7)ml、(8.5±2.0)min,组间差异均有统计学意义(P﹤0.05);两组患者并发症发生率比较,差异无统计学意义(P﹥0.05)。[结论]电视腹腔镜胆囊切除术临床效果更佳,患者术后恢复快,值得临床关注。

关键词：腹胆囊切除术电视腹腔镜小切口

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High Fe‑Loading Single‑Atom Catalyst Boosts ROS Production by Density Effect for Efficient Antibacterial Therapy

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Nano-Micro Letters 2025年第2期17卷 187-203页

作者： Si Chen Fang Huang Lijie mao Zhimin Zhang Han Lin Qixin Yan Xiangyu lu Jianlin Shi Department of Cardiology Shanghai Tenth People’s HospitalSchool of MedicineTongji UniversityShanghai 200092People’s Republic of China Shanghai Institute of Ceramics Chinese Academy of SciencesShanghai 200050People’s Republic of China Shanghai Frontiers Science Center of Nanocatalytic Medicine School of MedicineTongji UniversityShanghai 200092People’s Republic of China

The current single-atom catalysts(SACs)for medicine still suffer from the limited active site ***,we develop a synthetic method capable of increasing both the metal loading and mass-specific activity of SACs by exchanging zinc with *** constructed iron SACs(h^(3)-FNC)with a high metal loading of 6.27 wt%and an optimized adjacent Fe distance of~4 A exhibit excellent oxidase-like catalytic performance without significant activity decay after being stored for six months and promising antibacterial ***,a“density effect”has been found at a high-enough metal doping amount,at which individual active sites become close enough to interact with each other and alter the electronic structure,resulting in significantly boosted intrinsic activity of single-atomic iron sites in h^(3)-FNCs by 2.3 times compared to low-and medium-loading ***,the overall catalytic activity of h^(3)-FNC is highly improved,with mass activity and metal mass-specific activity that are,respectively,66 and 315 times higher than those of commercial Pt/*** addition,h^(3)-FNCs demonstrate efficiently enhanced capability in catalyzing oxygen reduction into superoxide anion(O_(2)·^(−))and glutathione(GSH)*** in vitro and in vivo assays demonstrate the superior antibacterial efficacy of h^(3)-FNCs in promoting wound *** work presents an intriguing activity-enhancement effect in catalysts and exhibits impressive therapeutic efficacy in combating bacterial infections.

关键词： Nanocatalytic medicine Single-atom catalysts Reactive oxygen species(ROS) High metal loading Oxidase catalysis

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Repetitive traumatic brain injury–induced complement C1–related inflammation impairs long-term hippocampal neurogenesis

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Neural Regeneration Research 2025年第3期20卷 821-835页

作者： Jing Wang Bing Zhang Lanfang Li Xiaomei Tang Jinyu Zeng Yige song Chao Xu Kai Zhao Guoqiang Liu Youming lu Xinyan Li Kai Shu Department of Neurosurgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Neurosurgery Shanxi Bethune HospitalShanxi Academy of Medical SciencesTongji Shanxi HospitalThird Hospital of Shanxi Medical UniversityTaiyuanShanxi ProvinceChina Department of Pathophysiology School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Institute for Brain Research Wuhan Center of Brain ScienceHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Graduate Student Chongqing Medical UniversityChongqingChina Department of Basic Medicine School of Medical ScienceHubei University for NationalitiesEnshiHubei ProvinceChina Department of Anatomy School of Basic Medicine and Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

Repetitive traumatic brain injury impacts adult neurogenesis in the hippocampal dentate gyrus,leading to long-term cognitive ***,the mechanism underlying this neurogenesis impairment remains *** this study,we established a male mouse model of repetitive traumatic brain injury and performed long-term evaluation of neurogenesis of the hippocampal dentate gyrus after repetitive traumatic brain *** results showed that repetitive traumatic brain injury inhibited neural stem cell proliferation and development,delayed neuronal maturation,and reduced the complexity of neuronal dendrites and *** with repetitive traumatic brain injuryalso showed deficits in spatial memory ***,following repetitive traumatic brain injury,neuroinflammation was enhanced in the neurogenesis microenvironment where C1q levels were increased,C1q binding protein levels were decreased,and canonical Wnt/β-catenin signaling was *** inhibitor of C1 reversed the long-term impairment of neurogenesis induced by repetitive traumatic brain injury and improved neurological *** findings suggest that repetitive traumatic brain injury–induced C1-related inflammation impairs long-term neurogenesis in the dentate gyrus and contributes to spatial memory retrieval dysfunction.

关键词： complement C1 dendrite dentate gyrus hippocampus neural stem cell neurogenesis neuroinflammation neurological function neuron traumatic brain injury

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经脐单孔腹腔镜与传统腹腔镜胆囊切除术的疗效对比观察

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中华医院感染学杂志 2011年第18期21卷 3851-3853页

作者：卢茂松黄钲焘曾鹏飞遵义医学院第三附属医院肝胆外科贵州遵义563002

目的对照研究经脐单孔腹腔镜与传统腹腔镜胆囊切除术的疗效。方法选择具有择期胆囊切除术手术指征者139例随机分治疗组82例、对照组57例,分别进行经脐单孔腹腔镜与传统腹腔镜胆囊切除术;分析其手术时间、术中出血量、下床活动时间、术... 详细信息

目的对照研究经脐单孔腹腔镜与传统腹腔镜胆囊切除术的疗效。方法选择具有择期胆囊切除术手术指征者139例随机分治疗组82例、对照组57例,分别进行经脐单孔腹腔镜与传统腹腔镜胆囊切除术;分析其手术时间、术中出血量、下床活动时间、术后住院天数及并发症发生率;1个月后对患者进行腹壁创口满意度调查;并进行统计分析。结果治疗者与对照组手术时间分别为(52.1±6.7)min、(33.8±7.2)min;术中出血量(39.2±12.5)ml、(42.7±11.9)ml;下床活动时间(14.3±6.5)h、(12.6±6.7)h;术后住院天数(5.7±1.3)d、(5.2±1.1)d;并发症发生率2.44%、1.75%,2组手术时间增加比较,差异有统计学意义(P<0.05),其他差异均无统计学意义;1个月后随访2组患者对腹壁创口满意率分别为96.34%和8.77%,差异有统计学意义(P<0.05)。结论经脐单孔腹腔镜与传统腹腔镜胆囊切除术,除手术时间增加外其疗效相仿,切口愈合良好,疤痕隐蔽,使腹壁创口能真正达到美的效果。

关键词：经脐单孔腹腔镜传统腹腔镜胆囊切除术

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Inhibition of the NLRP3 inflammasome attenuates spiral ganglion neuron degeneration in aminoglycoside-induced hearing loss

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Neural Regeneration Research 2025年第10期20卷 3025-3039页

作者： Jia Fang Zhuangzhuang Li Pengjun Wang Xiaoxu Zhang song mao Yini Li Dongzhen Yu Xiaoyan Li Yazhi Xing Haibo Shi Shankai Yin Department of Otolaryngology Head and Neck Surgery Shanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of MedicineOtolaryngology Institute of Shanghai Jiao Tong UniversityShanghai Key Laboratory of Sleep Disordered BreathingShanghaiChina Department of Otorhinolaryngology-Head and Neck Surgery Shanghai Children’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina

Aminoglycosides are a widely used class of antibacterials renowned for their effectiveness and broad antimicrobial ***,their use leads to irreversible hearing damage by causing apoptosis of hair cells as their direct *** addition,the hearing damage caused by aminoglycosides involves damage of spiral ganglion neurons upon *** investigate the mechanisms underlying spiral ganglion neuron degeneration induced by aminoglycosides,we used a C57BL/6J mouse model treated with *** found that the mice exhibited auditory deficits following the acute loss of outer hair *** ganglion neurons displayed hallmarks of pyroptosis and exhibited progressive degeneration over *** profiling of these neurons showed significant upregulation of genes associated with inflammation and immune response,particularly those related to the NLRP3 *** of the canonical pyroptotic pathway in spiral ganglion neurons was observed,accompanied by infiltration of macrophages and the release of proinflammatory *** intervention targeting NLRP3 using Mcc950 and genetic intervention using NLRP3 knockout ameliorated spiral ganglion neuron degeneration in the injury *** findings suggest that NLRP3 inflammasome-mediated pyroptosis plays a role in aminoglycoside-induced spiral ganglion neuron *** of this pathway may offer a potential therapeutic strategy for treating sensorineural hearing loss by reducing spiral ganglion neuron degeneration.

关键词： degeneration hearing loss macrophages Mcc950 neuroinflammation NLRP3 inflammasome ototoxicity pyroptosis sensorineural hearing loss spiral ganglion neuron

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Universal design of three-dimensional porous graphene-iron based promotors for kinetically rationalized lithium-sulfur chemistry

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Journal of Energy Chemistry 2025年第1期100卷 192-200页

作者： Hua Gao Yunfeng Zhang Menglei Wang Ruoxuan Yang Shuai Feng Xuan Cao Yaping Zhang Zhongyuan lu Yingze song State Key Laboratory of Environment-Friendly Energy Materials School of Materials and Chemistry Southwest University of Science and Technology School of Material Science and Engineering Henan University of Technology College of Chemistry and Chemical Engineering Taishan University

Lithium-sulfur （Li-S） batteries are widely deemed to be one of the most potential candidates for future secondary batteries because of their remarkable energy ***,notorious polysulfide shuttling and retarded sulfur reaction kinetics pose significant obstacles to the further application of Li-S *** rationally designed highly active electrocatalysts can facilitate polysulfide conversion,the universal and scalable synthesis strategies need to be ***,a universal synthetic strategy to construct a series of three-dimensional （3D） porous graphene-iron （3DGr-Fe） based electrocatalysts involving 3DGr-FeP,3DGr-Fe3C,and 3DGr-Fe3Se4is exploited for manipulating the Li-S redox *** has been observed that the implementation of a 3D porous Gr architecture leads to the well-designed conductive networks,while the uniformly dispersed iron nanoparticles introduce an abundance of active sites,fostering the lithium polysulfide conversion,thereby bolstering the overall electrochemical *** Li-S battery with the 3DGr-Fe based electrocatalyst exhibits remarkable capacity retention of 94.8%upon 100 times at 0.2 ***,the soft-packaged Li-S pouch cell based on such a 3DGr-Fe electrocatalyst delivers superior capacity of 1060.71 mA h g-1and guarantees for the continuous 30 min work of fan *** investigation gives comprehensive insights into the design,synthesis,and mechanism of 3DGr-Fe based electrocatalysts with high activity toward efficient and durable Li-S batteries.

关键词： Lithium-sulfur battery Universal synthesis 3D graphene-iron based promotor Electrocatalysis

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琯溪蜜柚亚洲柑橘叶点霉与首都叶点霉ITS-RFLP快速鉴定

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福建农业学报 2018年第2期33卷 195-198页

作者：罗金水郑域茹卢松茂林智明林艺华福建省热带作物科学研究所福建漳州363001

亚洲柑橘叶点霉Phyllosticta citriasiana是引起琯溪蜜柚黑斑病的病原菌。首都叶点霉Phyllosticta capitalensis是分离自琯溪蜜柚的一种腐生真菌。在本研究建立2种叶点霉属真菌的ITS-RFLP快速鉴定方法。首先采集平和县琯溪蜜柚样品,分... 详细信息

亚洲柑橘叶点霉Phyllosticta citriasiana是引起琯溪蜜柚黑斑病的病原菌。首都叶点霉Phyllosticta capitalensis是分离自琯溪蜜柚的一种腐生真菌。在本研究建立2种叶点霉属真菌的ITS-RFLP快速鉴定方法。首先采集平和县琯溪蜜柚样品,分离纯化获得目的菌***和***,提取这2种病菌的菌丝体总DNA,使用ITS1和ITS4引物对扩增rDNA-ITS基因片段,再用限制性内切酶Acc I酶切扩增产物。结果表明,***和***这2种菌的rDNA-ITS基因酶切分别产生了2个和3个特异条带。通过条带的数量和大小使这两种真菌得以区分,该方法快速、简便。

关键词：琯溪蜜柚黑斑病限制性内切酶片段长度多态性分析技术

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