检索结果-南通市图书馆

Analysis of the electron transfer pathway in small laccase by EPR and UV-vis spectroscopy coupled with redox titration

在线全文

学校读者我要写书评

暂无评论

Magnetic Resonance Letters 2024年第3期4卷 52-59页

作者： lu Yu Aokun Liu jian Kuang Ruotong wei Zhiwen Wang Changlin Tian High Magnetic Field Laboratory Hefei Institutes of Physical ScienceChinese Academy of SciencesHefei230031China Department of Endocrinology Institute of Endocrine and Metabolic DiseaseThe First Affiliated Hospital of USTCDivision of Life Sciences and MedicineHefei National Research Center for Physical Sciences at the MicroscaleJoint Center for Biological Analytical ChemistryAnhui Engineering Laboratory of Peptide DrugAnhui Laboratory of Advanced Photonic Science and TechnologyUniversity of Science and Technology of ChinaHefei230026China

Bacterial small laccases(SLAC) are promising industrial biocatalysts due to their ability to oxidize a broad range of substrates with exceptional thermostability and tolerance for alkaline p H. Electron transfer between substrate, copper centers, and O2is one of the key steps in the catalytic turnover of SLAC. However, limited research has been conducted on the electron transfer pathway of SLAC and SLAC-catalyzed reactions, hindering further engineering of SLAC to produce tunable biocatalysts for novel applications. Herein, the combinational use of electron paramagnetic resonance(EPR) and ultraviolet-visible(UV-vis) spectroscopic methods coupled with redox titration were employed to monitor the electron transfer processes and obtain further insights into the electron transfer pathway in SLAC. The reduction potentials for type 1 copper(T1Cu), type 2 copper(T2Cu) and type 3copper(T3Cu) were determined to be 367 ± 2 mV, 378 ± 5 m V and 403 ± 2 mV,respectively. Moreover, the reduction potential of a selected substrate of SLAC, hydroquinone(HQ), was determined to be 288 mV using cyclic voltammetry(CV). In this way, an electron transfer pathway was identified based on the reduction potentials. Specifically,electrons are transferred from HQ to T1Cu, then to T2Cu and T3Cu, and finally to ***, superhyperfine splitting observed via EPR during redox titration indicated a modification in the covalency of T2Cu upon electron uptake, suggesting a conformational alteration in the protein environment surrounding the copper sites, which could potentially influence the reduction potential of the copper sites during catalytic processes. The results presented here not only provide a comprehensive method for analyzing the electron transfer pathway in metalloenzymes through reduction potential measurements, but also offer valuable insights for further engineering and directed evolution studies of SLAC in the aim for biotechnological and industrial applications.

关键词： Electron paramagnetic resonance Redox titration Electron transfer Reduction Potential Small laccase

Geometric phase-encoded stimuli-responsive cholesteric liquid crystals for visualizing real-time remote monitoring:humidity sensing as a proof of concept

在线全文

学校读者我要写书评

暂无评论

Light(Science & Applications) 2024年第2期13卷 283-293页

作者： Shi-Long Li Zhao-Yi Chen Peng Chen wei Hu Chaohong Huang Sen-Sen Li Xuejia Hu Yan-Qing lu lu-jian Chen Department of Electronic Engineering School of Electronic Science and EngineeringXiamen University361005 XiamenChina College of Engineering and Applied Sciences Nanjing University210093 NanjingChina Fujian Key Laboratory of Ultrafast Laser Technology and Applications Xiamen University361005 XiamenChina

Liquid crystals are a vital component of modern photonics,and recent studies have demonstrated the exceptional sensing properties of stimuli-responsive cholesteric liquid ***,existing cholesteric liquid crystal-based sensors often rely on the naked eye perceptibility of structural color or the measurement of wavelength changes by spectrometric tools,which limits their practical ***,developing a platform that produces recognizable sensing signals is *** this study,we present a visual sensing platform based on geometric phase encoding of stimuli-responsive cholesteric liquid crystal polymers that generates real-time visual patterns,rather than frequency *** demonstrate this platform’s effectiveness,we used a humidity-responsive cholesteric liquid crystal polymer film encoded with a q-plate pattern,which revealed that humidity causes a shape change in the vortex beam reflected from the encoded cholesteric liquid crystal ***,we developed a prototype platform towards remote humidity monitoring benefiting from the high directionality and long-range transmission properties of laser beams carrying orbital angular *** approach provides a novel sensing platform for cholesteric liquid crystals-based sensors that offers promising practical *** ability to generate recognizable sensing signals through visual patterns offers a new level of practicality in the sensing field with stimuli-responsive cholesteric liquid *** platform might have significant implications for a broad readership and will be of interest to researchers working in the field of photonics and sensing technology.

关键词： humidity visual liquid

Effects of B, Mo, Zn, and Their Interactions on Seed Yield of Rapeseed (Brassica napus L.)

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2009年第1期19卷 53-59页

作者： YANG Mei SHI Lei XU Fang-Sen lu jian-wei WANG Yun-Hua Microelement Research Center Huazhong Agricultural University Wuhan 430070 （China）. National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 （China）

A field experiment was conducted to study the effects of boron (B), molybdenum (Mo), zinc (Zn) and their interactions on seed yield and yield formation of rapeseed (Brassica napus L. var. Huashuang 4). Application of B fertilizer to a sandy soil increased the seed yield by 46.1% compared to the control and also created a considerably higher seed yield than the two treatments solely applying Mo and Zn fertilizers, which suggested that B was a main constraint for the seed yield of Huashuang 4 in this experiment. The effect of B fertilizer on the seed yield was attributed to an increase in the number of seeds per silique and siliques per plant. The combined application of B with Mo or Zn resulted in higher seed yield than the application of B, Mo or Zn alone, and the seed yield of the B+Mo+Zn treatment was the highest in all treatments, 68.1% above the control. Dry matter accumulation of seed followed a typical S-shaped curve and it was higher in plants supplied with B than in plants without B. A small but significant increase in the seed oil content and an improvement in the oil quality were also observed in all treatments compared with the control. These results suggested that optimal micronutrient application could provide both yield and quality advantages for rapeseed in poor soil.

关键词： boron Brassica napus molybdenum seed yield zinc

Progress of vegetation modelling and future research prospects

在线全文

学校读者我要写书评

暂无评论

Science China Earth Sciences 2024年第9期67卷 2718-2738页

作者： Siqi LI Xu ZHANG Zhengyao lu jian NI jianhua lu State Key Laboratory of Tibetan Plateau Earth System Resources and Environment(TPESRE)Institute of Tibetan Plateau ResearchChinese Academy of SciencesBeijing 100101China College of Resources and Environment University of Chinese Academy of SciencesBeijing 100049China British Antarctic Survey Cambridge CB30ETUnited Kingdom Department of Physical Geography and Ecosystem Science Lund UniversityLund 22362Sweden College of Life Sciences Zhejiang Normal UniversityJinhua 321004China School of Atmospheric Sciences Sun Yat-sen University and Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)Zhuhai 519082China

Terrestrial vegetation is a crucial component of the Earth system,and its changes not only represent one of the most distinct aspects of climate change but also exert significant feedback within the climate system by exchanging energy,moisture,and carbon *** quantitatively and mechanistically study climate-vegetation feedback,numerical vegetation models have been developed on the theory of ecophysiological constraints on plant functional *** models eventually can simulate vegetation distribution and succession across different spatial and temporal scales,and associated terrestrial carbon cycle processes by categorizing vegetation into biomes according different plant functional types and their associated environmental *** we review the developing history of vegetation models and provide recent advances and future *** 21st century,static vegetation models,as developed statistical models,can only simulate equilibrated characteristics of vegetation *** last several decades,Dynamic Global Vegetation Models(DGVMs)have been developed to simulate instantaneous responses of vegetation to climate change and associated dynamics,and can be coupled with Earth system models to investigate interactions among atmosphere,ocean,and *** are also widely applied to investigate the dynamics accounting for changes in the geographic distribution patterns of land surface vegetation at different spatial and temporal scales and to assess the impacts of terrestrial carbon and water fluxes and land use *** suggest that future vegetation modeling could integrate with machine learning,and explore vegetation transient response and feedback as well as impacts of process hierarchies and human activities on climate and ecosystem.

关键词： Vegetation models DGVMs Ecosystems Plant functional types Plant functional traits

同方期刊数据库

Study on the Nutrition Characteristics of Different K Use Efficiency Cotton Genotypes to K Deficiency Stress

在线全文

学校读者我要写书评

暂无评论

Agricultural Sciences in China 2008年第6期7卷 740-745页

作者： jianG Cun-cang CHEN Fang GAO Xiang-zhao lu jian-wei WAN Kai-yuan NIAN Fu-zhao WANG Yun-hua Plant Nutrition Lab Huazhong Agricultural University Wuhan 430070 P.R.China Wuhan Botanical Garden Chinese Academy of Sciences Wuhan 430074 P.R.China National Agro-Tech Extension and Service Center Beijing 100026 P.R.China

To study the mechanism of plant K use efficiency, both K high and low use efficiency cotton genotypes, 103 and 122, respectively, were selected from 86 cotton cultivars （Gossypium hirsutum L.）. The research was conducted using pot experiment for planting cotton on without K （0.0 g kg-1 soil） and with K （0.4 g kg-1 soil） treatments in 2005. Experimental result showed that, with K deficiency stress, genotype 103 grew much better than genotype 122 except that its lower leaves showed the symptoms of K deficiency, whereas all the leaves of genotype 122 showed the symptoms of K deficiency. Root dry matter weights of treatments for genotype 103 with and without K application were 1.07 and 1.25 times of genotype 122. It indicated that the root system of genotype 103 was well developed and has better nutrition uptake capability than that of genotype 122. The result also showed that the cotton shoots of genotype 103 were 1.07 and 1.13 times over genotype 122 on treatments of with and without K application. It indicated that genotype 103 has stronger transport organs. In genotype 103, plants, dry matter, and potassium were mainly transported to cotton bolls. The boll dry weight of genotype 103 was 2.58 times in without K treatment and 1.90 times in with K treatment over genotype 122. The potassium accumulation in bolls of genotype 122 was only 49.3% of that in genotype 103. Potassium accumulation in the other organs of genotype 103 was relatively low compared with in bolls. This indicated that the distribution of K and organic matter in genotype 103 was more efficient than genotype 122. The main differences between high K efficiency cotton genotype 103 and low K efficiency genotype 122 lie in their potassium nutrition and organic matter using efficiency on uptake, transportation, accumulation, distribution, and utilization.

关键词： cotton (Gossypium hirsutum L.) potassium use efficiency genotype uptake capability distribution ability

Content of Nutritional Elements in Sudangrass and Ryegrass Determined by ICP-AES

在线全文

学校读者我要写书评

暂无评论

光谱学与光谱分析 2011年第9期31卷 2555-2557页

作者： LI Wen-xi lu jian-wei Saman P Seneweera WU Ji CHEN Fang lu Jun-ming LI Xiao-kun College of Resources and Environment Huazhong Agricultural University Wuhan 430070 China School of Agriculture and Food Systems Faculty of Land and Foodthe University of Melbourne Horsham Victoria 3401 Australia Institution of Soil and Fertilizer Anhui Academy of Agricultural Sciences Hefei 230031 China International Plant Nutrition Institute China Program Wuhan 430074 China Agricultural and Technical Center Datonghu Administration District of Honghu City Honghu 434300 China

The sudangrass(Sorghumsudanense) and ryegrass(Lolium multiflorum L.) rotation is a newtype of cropping system,which has developed rapidly in recent years in the south of *** contents ofnutritional elements for forage grass in the sudangrass and ryegrass rotation system were determined by *** results showed that there were abundant and essential nutritional elements for ani mals in sudangrassand *** contents of P,K,Ca,Mg,S,Fe,B,Cu,Zn and Mn for sudangrass were 0.20 %～0.29 %,1.94 %～2.57 %,0.62 %～0.97 %,0.39 %～0.69 %,0.12 %～0.18 %,108.35 ～180.12,3.04 ～5.96,6.17 ～10.02,20.37 ～31.36 and 46.80 ～101.29 ***-1,*** contents of P,K,Ca,Mg,S,Fe,B,Cu,Zn,Mn for ryegrass were 0.39 %～0.70 %,3.77 %～5.07 %,0.61 %～0.84 %,0.28 %～0.47 %,0.32 %～0.41 %,291.65 ～632.20,2.13 ～3.23,13.29 ～15.19,30.73 ～42.98 and 92.08 ～156.04 ***-1,respectively,and there were differences between various periods in nutritional elements inthe two forage *** application of ICP-AES could reflect fast and efficiently the content of nutritionalelements for forage grass as ani mals feed.

关键词： ICP-AES Sudangrass Ryegrass Nutritional elements

Pathogen-induced methylglyoxal negatively regulates rice bacterial blight resistance by inhibiting OsCDR1 protease activity

在线全文

学校读者我要写书评

暂无评论

Molecular Plant 2024年第2期17卷 325-341页

作者： Zheng-wei Fu jian-Hui Li Xiang Gao Shi-Jia Wang Ting-Ting Yuan Ying-Tang lu State Key Laboratory of Hybrid Rice College of Life SciencesRenmin Hospital of Wuhan UniversityWuhan UniversityWuhan 430072China Key Laboratory of Biology and Genetic Improvement of OilCrops Ministry of Agriculture and Rural AffairsOilCrops Research Institute of the Chinese Academy of Agricultural SciencesWuhan 430062China

Xanthomonas oryzae ***(Xoo)causes bacterial blight(BB),a globally devastating disease of rice(Oryza sativa)that is responsible for significant crop *** and sugar metabolites are important for pathogen infection,providing energy and regulating events associated with defense responses;howev-er,the mechanisms by which they regulate such events in BB are *** an inevitable sugar metabolite,methylglyoxal(MG)is involved in plant growth and responses to various abiotic stresses,but the underlying mechanisms remain *** and how MG functions in plant biotic stress responses is almost completely ***,we report that the Xoo strain PXO99 induces OsWRKY62.1 to repress transcrip-tion of OsGLY Il genes by directly binding to their promoters,resulting in overaccumulation of *** negatively regulates rice resistance against Pxo99:osglyll2 mutants with higher MG levels are more sus-ceptible to the pathogen,whereas OsGLYIl2-overexpressing plants with lower MG content show greater resistance than the wild *** of OsGLYll2 to prevent excessive MG accumulation confers broad-spectrum resistance against the biotrophic bacterial pathogens Xoo and Xanthomonas oryzae *** and the necrotrophic fungal pathogen Rhizoctonia solani,which causes rice sheath *** evidence shows that MG reduces rice resistance against PXO99 through CONSTITUTIVE DISEASE RESISTANCE 1(OsCDR1).MGmodifies the Arg97 residue of OsCDR1to inhibit its aspartic protease activ-ity,which is essential for OsCDR1-enhanced *** together,these findings illustrate how Xoo promotes infection by hijacking a sugar metabolite in the host plant.

关键词： Oryza sativa bacterial blight OsWRKY62.1 methylglyoxal OsCDR1

Characteristics of genetic basis copy number variation in production and adaptation traits of Chinese indigenous sheep

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Genetics and Genomics 2024年第5期51卷 566-569页

作者： Meilin Jin Gang Liu jian lu Zhiqiang Chen Huihua Wang Taotao Li Caihong wei State Key Laboratory of Animal Biotech Breeding Institute of Animal SciencesChinese Academy of Agricultural Sciences(CAAS)Beijing 100193China College of Animal Science and Technology China Agricultural UniversityBeijing100193China National Animal Husbandry Service National Center of Preservation&Utilization of Animal Genetic ResourcesBeijing 100125China Glbizzia Biosciences Beijing 102629China

Copy number variations(CNVs)range in size from 50 base pairs(bp)to several megabases(Mb)and encompass various types of structural variations,such as insertions,deletions,duplications,and complex multiallelic events(MacDonald et al.,2014).These genomic alterations modulate phenotype by altering gene dosage and transcriptional regulation,and are key drivers of biological evolution and determinants of genetic diversity and phenotypic *** recent years,CNVs have been detected in the genomes of various livestock species(Yuan et al.,2021;Chen et al.,2022).Upadhyay et al.(2017)conducted a comprehensive analysis of CNV distribution in the European cattle genome and determined that the Kit gene is associated with color-sidedness in English longhorn *** study presented extensive genome-wide information on CNVs in wild,commercial,and local chicken breeds to gain an integrated understanding of the domestication process and exclusively identified the genes involved(Chen et al.,2022).

关键词： sided number dosage

Optical scattering imaging with sub-nanometer precision based on position-ultra-sensitive giant Lamb shift

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Physics,Mechanics & Astronomy) 2024年第6期67卷 96-105页

作者： Zeyang Liao Yu-wei lu wei Li Xue-Hua Wang State Key Laboratory of Optoelectronic Materials and Technologies School of PhysicsSun Yat-sen UniversityGuangzhou 510275China Quantum Science Center of Guangdong-Hong Kong-Macao Greater Bay Area(Guangdong) Shenzhen 518045China

The Lamb shift of a quantum emitter in close proximity to a plasmonic nanostructure can be three or more orders of magnitude larger than that in the free space and is ultra-sensitive to the emitter position and *** demonstrate that this large Lamb shift can be sensitively observed from the scattering or absorption spectrum dip shift of the coupled system when the plasmonic nanoparticle or tip scans the *** these observations,we propose a scanning optical scattering imaging method based on the plasmonic-enhanced Lamb shift with achieves sub-nanometer *** method is based on the scattering or absorption spectrum of the plasmon-emitter coupling system,which is free of the fluorescence quenching problem and easier to implement in a plasmon-emitter coupling *** addition,our scheme works even if the quantum emitter is slightly below the dielectric surface,which can bring about broader applications,such as detecting atoms and molecules or quantum dots above or under a surface.

关键词： Lamb shift plasmon optical scattering imaging