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Inhibitory effects of miRNA-200c on chemotherapy-resistance and cell proliferation of gastric cancer SGC7901/DDP cells

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Chinese Journal of Cancer 2010年第12期29卷 1006-1011页

作者： Yong Chen Jing Zuo ying liu Hong gao Wei liu Department of Medical Oncology Hebei Medical University Shijiazhuang Hebei 050011 P. R. China

Background and Objective: miRNA-200c can not only inhibit the aggressiveness of cancer cells but also increase the sensitivity of cells to antitumor drugs. However, some mechanisms are still unclear. Recent researches revealed that E-cadherin is more than an inhibitor of metastasis, and it also plays important roles in reversing drug resistance. We had previously found that miRNA-200c could not only induce the expression of E-cadherin but also increase the sensitivity of gastric cancer SGC7901/DDP cells to cisplatin (DDP). This study aimed to explore the effects of miRNA-200c on biological characteristics of SGC7901/DDP cells and the roles of E-cadherin in the regulatory pathway of miRNA-200c. Methods: SGC7901/DDP cells and its parental cell line SGC7901 cells were transfected with miRNA-200c precursor (Pre-200c) and E-cadherin siRNA, respectively. Real-time RT-PCR was used to detect miRNA-200c expression after transfection with Pre-200c in SGC7901/DDP cell line. Drug sensitivities to DDP, 5-fluorouracil (5-FU), paclitaxel, and adriamycin (ADR) after transfection were tested using MTT assay. The proliferation of SGC7901/DDP cells was also detected after transfection. The protein changes of E-cadherin, Bax, and Bcl-2 after transfection were detected by Western blot. Results: The miRNA-200c expression in SGC7901/DDP cells after transfection of Pre-200c was 7.128 ± 0.159 times of that in negative control (P < 0.05). The IC50 of DDP, 5-FU, paclitaxel, and ADR in Pre-200c-transfected group were significantly lower than that in negative control group (P < 0.05). Compared to the control group, cell proliferation was significantly decreased (P < 0.05). The relative protein expressions of E-cadherin and Bax in Pre-200c-transfected group were significantly higher than those in negative control group (P < 0.05), whereas Bcl-2 was significantly lower than that in control (P < 0.05). Additionally, E-cadherin protein expression was significantly inhibited after transfected with E

关键词： miRNA 增殖细胞胃癌细胞 C型 siRNA 细胞转染钙粘蛋白化疗

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The Growth Analysis in the Kernel Filling Process of K-Type Hybrid 901 and Its Parent Wheat

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Agricultural Sciences in China 2005年第12期4卷 903-908页

作者： GONG Yue-hua liu ying-zhou gao Jun-feng College of Life Science Northwest A ＆ F University Yangling 712100 PR.China

Using Matlab software, the grain filling process of hybrid wheat 901 and its parents was fitted by Richards equation W = A/（1 ＋ Be^-kt in order to study the characteristics of grain filling of the hybrid. The active grain growth period of the hybrid was 6 d longer than that of Shaan 229, and its final grain weight （43.7 g/1 000 grains） was higher than that of Shaan229 （36.3 g/1 000 grains）. N values of 901 and R205 were both less than 1, and their grain growth was faster in the early filling stage, while slower in the middle-late stage. N value of Shaan 229 was 〉1~ and its growth was slower in the early stage and faster in the middle stage. The period of early stage of 901 was shorter and of middle-late stage was longer. The situation of Shaan 229 was totally reversed. For parents, the father plant R205 was similar to hybrid wheat 901, whereas its mother plant K3314A similar to Shaan 229. It has been found that Richards equation was more suitable for fitting the grain filling process of wheat than Logistic equation.

关键词： Hybrid wheat 901 Parent plants Grain-filling

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OsBGLU19 and OsBGLU23 regulate disease resistance to bacterial leaf streak in rice

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Journal of Integrative Agriculture 2019年第6期18卷 1199-1210页

作者： LI Bei-bei liu ying-gao WU Tao WANG Ji-peng XIE Gui-rong CHU Zhao-hui DING Xin-hua State Key Laboratory of Crop Biology/Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests Shandong Agricultural University

β-Glucosidase belongs to the glycoside hydrolase I family,which is widely present in multiple species and responds to various biotic and abiotic *** rice,whetherβ-glucosidase is involved in the interaction between plants and microorganisms is not *** this study,we found that the expression of several genes encodingβ-glucosidases,including OsBGLU19 and OsBGLU23,were upregulated after inoculation with Xanthomonas oryzae ***(Xoc)and downregulated after inoculation with *** ***(Xoo).The respective insertion mutants of OsBGLU19 and OsBGLU23,bglu19 and bglu23,were more susceptible to Xoc *** expression of OsAOS2,a key gene in the jasmonic acid signal pathway,was dramatically downregulated after inoculation with Xoc in the bglu19 and bglu23 ***,the expression of downstream disease resistance-related genes,such as OsPR1a,OsPR5 and a key transcription factors OsWRKY72 were obviously *** resistance mediated by OsBGLU19 and OsBGLU23 to bacterial leaf streak is related to disease resistance-related genes above mentioned.

关键词： β-glucosidase OsBGLU19 OsBGLU23 Oryza sativa bacterial leaf streak

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Promotion of Project-Based Learning Aimed at Professional Skills

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Sino-US English Teaching 2016年第1期13卷 59-62页

作者： liu Li gao ying Dalian University of Technology Dalian China

Project-based learning theory is applied in Software English course. The aim is to develop students＇ professional skill. A professional skill scale of software engineer is introduced with Software English communicative skill （reading skill, writing skill, listening skill, and communicative skill）, engineering thinking habit （skill of design the plan and problem-solving skill）, engineering thinking skill, knowledge of software engineering, and Emotion Quotient to deal with problems （Team spirit and communicative skill and self-assessment skill）. For software majors, students learn about five stages and job responsibilities in software making process.

关键词： project-based learning professional skill scale Software English EOP

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The Current Situation of the Reform of College English Teaching In our University——A Survey on Different Teaching Methods and Students' Learning Attitude towards English

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江西理工大学学报 2004年第6期26卷 80-82页

作者： liu Hong-ying gao Fei

The 21st century witnesses the enforcement of the world’s communication and ***,as an essen-tial ability in international exchanges,has become a basic requirement for all the young people at *** ago,the employers,when they wanted to employ someone,required that he or she should gain a certificate ofCollege English Test,Band Four or Band Six or *** now they not only require young people to gain these cer-

关键词：外语教学与研究大学英语教学改革外语教学改革非英语专业大学生教学原则学习者为中心中国高校学习动机外语界大学英语课

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Multi boron-doping effects in hard carbon toward enhanced sodium ion storage

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Journal of Energy Chemistry 2025年第1期100卷 730-738页

作者： Peng Zheng Wang Zhou ying Mo Biao Zheng Miaomiao Han Qin Zhong Wenwen Yang Peng gao Lezhi Yang Jilei liu College of Materials Science and Engineering Hunan Joint International Laboratory of Advanced Materials and Technology for Clean Energy Hunan Province Key Laboratory for Advanced Carbon Materials and Applied Technology Hunan University Greater Bay Area Institute for Innovation Hunan University Changsha Research Institute of Mining & Metallurgy Co. LTD.

Hard carbon （HC） has been considered as promising anode material for sodium-ion batteries （SIBs）.The optimization of hard carbon’s microstructure and solid electrolyte interface （SEI） property are demonstrated effective in enhancing the Na+storage capability,however,a one-step regulation strategy to achieve simultaneous multi-scale structures optimization is highly ***,we have systematically investigated the effects of boron doping on hard carbon’s microstructure and interface chemistry.A variety of structure characterizations show that appropriate amount of boron doping can increase the size of closed pores via rearrangement of carbon layers with improved graphitization degree,which provides more Na+storage ***-situ Fourier transform infrared spectroscopy/electrochemical impedance spectroscopy （FTIR/EIS） and X-ray photoelectron spectroscopy （XPS） analysis demonstrate the presence of more BC3and less B–C–O structures that result in enhanced ion diffusion kinetics and the formation of inorganic rich and robust SEI,which leads to facilitated charge transfer and excellent rate *** a result,the hard carbon anode with optimized boron doping content exhibits enhanced rate and cycling *** general,this work unravels the critical role of boron doping in optimizing the pore structure,interface chemistry and diffusion kinetics of hard carbon,which enables rational design of sodium-ion battery anode with enhanced Na+storage performance.

关键词： X ray photoelectron spectroscopy

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Preliminary Design of a System for Picking up Audio-Signals From Bubble Detectors gao Si-ze1,NI Bang-fa,ZHANG Gui-ying,XIAO Cai-jin,liu Cun-xiong, CHEN Zhe1,NIE Peng,liu Chao1,ZHAO Chang-jun (*** of Physics Science and Engineering Technology,Guang

Preliminary Design of a System for Picking up Audio-Signals ...

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作者： gao Si-ze NI Bang-fa ZHANG Gui-ying XIAO Cai-jin liu Cun-xiong CHEN Zhe NIE Peng liu Chao ZHAO Chang-jun College of Physics Science and Engineering Technology Guangxi University

This system is designed to pick up sound signals from large volume,low-threshold energy, low-background superheated droplets bubble detectors.A sound-sensitive bubble detector is composed of a container filled with a mixture of superheated droplets and gel,a temperature and pressure control system,and an audio acquisition system attached on the outer surface of the *** superheated droplets are in a metastable and superheated state at ambient temperature and *** the outside

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柔性石墨密封材料在铝液质量检测装置中的应用

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特种铸造及有色合金 2003年第2期23卷 59-61页

作者：高洪吾秦颖刘顺华高钦大连理工大学

通过在铝液质量检测系统中使用的真空密封材料的效果表明 ,柔性石墨材料在 80 0℃高温条件工作 ,化学稳定性良好 ,不污染铝液 ,密封性和使用性能完全满足铝液检测的要求。系统处于真空条件下 ,柔性石墨的氧化不明显 ,非密封端面则不影... 详细信息

通过在铝液质量检测系统中使用的真空密封材料的效果表明 ,柔性石墨材料在 80 0℃高温条件工作 ,化学稳定性良好 ,不污染铝液 ,密封性和使用性能完全满足铝液检测的要求。系统处于真空条件下 ,柔性石墨的氧化不明显 ,非密封端面则不影响系统的密封性。

关键词：柔性石墨真空密封检测铝

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Low-cost and fluoride-free synthesis of MFI zeolite nanosheets with enhanced stability for benzene alkylation with ethanol

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Journal of Energy Chemistry 2025年第1期100卷 458-468页

作者： Peng Zhu Cun liu Yiren Yu Guoshu gao Yumeng Zhao Xiongfu Zhang Guodong liu Guohui Yang State Key Laboratory of Fine Chemicals School of Chemical Engineering Dalian University of Technology State Key Laboratory of Catalysis Dalian Institute of Chemical Physics Chinese Academy of Sciences

Zeolite nanosheets with a short b-axis thickness are highly desirable in lots of catalytic reactions due to their reduced diffusion resistance. Nevertheless, conventional synthesis methods usually require expensive structure-directing agents(SDAs), pricey raw materials, and eco-unfriendly fluorine-containing additives. Here, we contributed a cost-effective and fluoride-free synthesis method for synthesizing high-quality MFI zeolite nanosheets through a Silicalite-1(Sil-1) seed suspension and urea cooperative strategy, only with inexpensive colloidal silica as the Si source. Our approach was effective for synthesizing both Sil-1 and aluminum-containing ZSM-5 nanosheets. By optimizing key synthesis parameters,including seed aging time, seed quantity, and urea concentration, we achieved precise control over the crystal face aspect ratio and b-axis thickness. We also revealed a non-classical oriented nanosheet growth mechanism, where Sil-1 seeds induced the formation of quasi-ordered precursor particles, and the(010)crystal planes of these particles facilitated urea adsorption, thereby promoting c-axis-oriented *** obtained ZSM-5 nanosheets exhibited exceptional catalytic performance in the benzene alkylation with ethanol, maintaining stability for over 500 h, which is 5 times longer than traditional ZSM-5 catalysts. Furthermore, large-scale production of ZSM-5 nanosheets was successfully carried out in a 3 L highpressure autoclave, yielding samples consistent with those from laboratory-scale synthesis. This work marks a significant step forward in the sustainable and efficient production of MFI nanosheets using inexpensive and environmentally friendly raw materials, offering the broad applicability in catalysis.

关键词： MFI zeolite ZSM-5 Nanosheets Scale-up synthesis Aromatic alkylation

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Testing of the Splitting rate of PET/PA Composite Microfiber

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科技信息 2007年第27期 177-178页

作者： Yanyan liu Ming gao ying Dong Ping Zhu(State Key Laboratory for New Fiber Material and Modern Textile of Qingdao University,Qingdao266071) State Key Laboratory for New Fiber Material and Modern Textile of Qingdao University Qingdao266071

The PET/PA composite microfiber yarn was cut into slices,which were viewed with an optical microscope and photographed with a digital *** gap-splitting rate and dislocated-splitting rate observed within the pictures were then *** the help of Matlab7.0 program,the results of splitting rate virtual values can be obtained with integral *** creates a new testing method for determining the splitting rate of polyester and nylon composite microfiber fabric.

关键词： composite microfiber dislocate-splitting rate gap-splitting rate

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