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Experimental study on mechanical properties of basalt fiber-reinforced silty clay

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Journal of Central South university 2022年第6期29卷 1945-1956页

作者： JIA Yu ZHANG Jia-sheng {1. Xuan DING Yu CHEN xiao-bin {3. Tao Geotechnical engineering School of Civil EngineeringCentral South UniversityChangsha 410083China National engineering Research Center of High-Speed Railway Construction {1. Central South UniversityChangsha 410083China china Railway Siyuan {1. and Design Group Co. Ltd.Wuhan 430060China

Fiber reinforcement technology can significantly improve the mechanical properties of soil and has been increasingly app{1.ed in geotechnical *** fiber is a new kind of environment-friendly and highperformance soil reinforcement material,and the mechanical properties of basalt fiber-reinforced soil have become a hot research *** this paper,we conducted monotonic triaxial and cyc{1.c triaxial tests,and analyzed the influence of the fiber content,moisture content,and confining pressure on the shear characteristics,dynamic modulus,and damping ratio of basalt fiber-reinforced silty *** results illustrate that basalt fiber can enhance the shear strength of silty clay by increasing its *** find that the shear strength of reinforced silty clay reaches its maximum when the fiber content is approximately 0.2%and the moisture content is 1..5%(optimum moisture content).Similarly,we also find that the dynamic modulus that corresponds to the same strain first increases then decreases with increasing fiber content and moisture content and reaches its maximum when the fiber content is approximately 0.2%and the moisture content is 1..5%.The dynamic modulus is positively correlated with the confining ***,the change in the damping ratio with fiber content,moisture content,and confining pressure is opposite to that of the dynamic *** can be concluded that the optimum content of basalt fiber for use in silty clay is 0.2%.After our experiments,we used scanning electron microscope(SEM)to observe the microstructure of specimens with different fiber contents,and our results show that the gripping effect and binding effect are the main mechanisms of fiber reinforcement.

关键词： basalt fiber-reinforced silty clay shear behavior dynamic modulus damping ratio optimum fiber content

Cloning and characterization of an oxiranedicarboxylate hydrolase from Labrys sp. WH-1

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Journal of zhejiang {1.-Science B(Biomedicine & Biotechnology) 2019年第12期20卷 995-1002页

作者： Wen-na BAO Zi-sheng LUO Shi-{1. liu Yuan-feng WU Pei-{3.an WEI Gong-nian xiao Yong liu College of Biosystems engineering and Food Science Zhejiang UniversityHangzhou 310058China school of Biological and Chemical {1. Zhejiang University of Science and TechnologyHangzhou 310023China zhejiang Provincial Key Laboratory for Chemical and Biological Processing {1. of Farm Products Hangzhou 310023China

Objective:This study aimed to clone and characterize the oxiranedicarboxylate hydrolase(ORCH) from Labrys ***:Purification by column chromatography,characterization of enzymatic properties,gene cloning by protein terminal sequencing and polymerase chain reaction(PCR),and sequence analysis by secondary structure prediction and multiple sequence alignment were ***:The ORCH from Labrys ***-1.was purified 26-fold with a yield of 1..7%.It is a monomer with an isoelectric point(pl) of 8.57 and molecular mass of 3..2 *** was stable up to 55℃with temperature at which the activity of the enzyme decreased by 50% in 1. min(T501.) of 61.and the half-life at 50℃(t1.2,50℃) of 51.min and was also stable from pH 4 to 1.,with maximum activity at 55℃and pH *** is a metal-independent enzyme and strongly inhibited by Cu2+,Ag+,and anionic *** kinetic parameters(Km,kcat,and kcat/Km) were 1..7 mmol/L,222.3.s-1.and 1..9 mmol/(L s),*** ORCH gene,which contained an open reading frame(ORF) of 825 bp encoding 274 amino acid residues,was overexpressed in Escherichia coli and the enzyme activity was 3. times higher than that of the wild *** The catalytic efficiency and thermal stability of the ORCH from Labrys ***-1.were the best among the reported ORCHs,and it provides an alternative catalyst for preparation of L(+)-2,3.dihydrobutanedioic acid.

关键词： Oxiranedicarboxylate hydrolase(ORCH) L(+)-2,3.Dihydrobutanedioic acid Characterization Cloning

The phenylalanine ammonia-lyase gene family in Isatis indigotica Fort.:molecular cloning,characterization,and expression analysis

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Chinese Journal of Natural Medicines 2016年第11期14卷 801-812页

作者： MA Rui-Fang liu Qian-Zi {1. Ying ZHANG Lei li Qing YIN Jun CHEN Wan-{3. Development and Utilization Key Laboratory of Northeast Plant Materials School of Traditional Chinese Materia MedicaShenyang Pharmaceutical University College of Pharmacy Jiamusi University Department of Pharmacy Changzheng Hospital Second Military Medical University Department of Pharmaceutical Botany School of Pharmacy Second Military Medical University

Phenolic compounds, metabolites of the phenylpropanoid pathway, play an important role in the growth and environmental adaptation of many plants. Phenylalanine ammonia-lyase(PAL) is the first key enzyme of the phenylpropanoid pathway. The present study was designed to investigate whether there is a multi-gene family in I. Indigotic and, if so, to characterize their properties. We conducted a comprehensive survey on the transcription profiling database by using tBLASTn analysis. Several bioinformatics methods were employed to perform the prediction of composition and physicochemical characters. The expression levels of IiPAL genes in various tissues of I. indigotica with stress treatment were examined by quantitative real-time PCR. Protoplast transient transformation was used to observe the locations of IiPALs. IiPALs were functionally characterized by expression with pET-3.a vector in Escherichia colis strain BL21(DE3.. Integration of transcripts and metabolite accumulations was used to reveal the relation between IiPALs and target compounds. An new gene(IiPAL2) was identified and both IiPALs had the conserved enzymatic active site Ala-Ser-Gly and were classified as members of dicotyledon. IiPAL1 and IiPAL2 were expressed in roots, stems, leaves, and flowers, with the highest expression levels of IiPAL1 and IiPAL2 being observed in stems and roots, respectively. The two genes responded to the exogenous elicitor in different manners. Subcellular localization experiment showed that both IiPALs were localized in the cytosol. The recombinant proteins were shown to catalyze the conversion of L-Phe to trans-cinnamic acid. Correlation analysis indicated that Ii PAL1 was more close to the biosynthesis of secondary metabolites than IiPAL2. In conclusion, the present study provides a basis for the elucidation of the role of Ii PALs genes in the biosynthesis of phenolic compounds, which will help further metabolic engineering to improve the accumulation of bioactive components

关键词： Phenylalanine ammonia-lyase Isatis indigotica Fort Phenolic compounds lignan Correlation analysis

Transcriptional activation and phosphorylation of OsCNGC9 confer enhanced chilling tolerance in rice

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Molecular Plant 2021年第2期14卷 315-329页

作者： Jiachang wang Yulong Ren Xi {1. sheng Luo {3. Zhang Xin {1. Qibing lin Shanshan Zhu Hua Wan Yang Yang Yu Zhang Bin Lei Chunlei Zhou Tian Pan Yongfei wang Mingming Wu Ruonan jing Yang Xu Meng Han Fuqing Wu Cailin Lei Xiuping Guo Zhijun Cheng {3.ming zheng Yihua wang Zhigang Zhao ling Jiang Xin Zhang Yong-Fei wang Haiyang wang Jianmin Wan National Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China National Key Laboratory of Plant Molecular Genetics Institute of Plant Physiology and EcologyCAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China MOE Key Laboratory of Bioinformatics School of Life SciencesTsinghua UniversityBeijing100084China

Low temperature is a major environmental factor that limits plant growth and *** transient elevation of cytoplasmic calcium has long been recognized as a critical signal for plant cold tolerance,the calcium channels responsible for this process have remained largely *** we report that OsCNGC9,a cyclic nucleotide-gated channel,positively regulates chilling tolerance by media{3. cytoplasmic calcium elevation in rice(Oryza sativa).We showed that the loss-of-function mutant of OsCNGC9 is defective in cold-induced calcium influx and more sensitive to prolonged cold treatment,whereas OsCNGC9 overexpression confers enhanced cold ***,we demonstrated that in response to chilling stress,OsSAPK8,a homolog of Arabidopsis thaliana OST1.phosphorylates and activates OsCNGC9 to trigger Ca2+***,we found that the transcription of OsCNGC9 is activated by a rice dehydration-responsive element-binding transcription factor,*** together,our results suggest that OsCNGC9 enhances chilling tolerance in rice through regula{3. cold-induced calcium influx and cytoplasmic calcium elevation.

关键词： OsCNGC9 OsSAPK8 OsDREB1. cold signaling transduction chilling tolerance

NiO-SiO_2,NiO-Al_2O_3.NiO-Al_2O_3.SiO_2催化剂上顺酐选择加氢性能的比较

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燃料化学学报 2003年第3期31卷 263-266页

作者：赵永祥秦晓琴武志刚许临萍刘滇生山西大学化学化工学院山西太原030006

以正硅酸乙酯和硝酸铝分别为硅源和铝源,硝酸镍为活性组分前驱物,采用溶胶凝胶法制备了NiO SiO2,NiO Al2O3.NiO Al2O3.SiO2催化剂。顺酐液相选择加氢活性和选择性评价结果表明:顺酐在三种催化剂上转化率都在99%以上,而产物的选择性有... 详细信息

以正硅酸乙酯和硝酸铝分别为硅源和铝源,硝酸镍为活性组分前驱物,采用溶胶凝胶法制备了NiO SiO2,NiO Al2O3.NiO Al2O3.SiO2催化剂。顺酐液相选择加氢活性和选择性评价结果表明:顺酐在三种催化剂上转化率都在99%以上,而产物的选择性有较大的差别,其中NiO SiO2催化剂上γ 丁内酯的选择性达80 1.,NiO Al2O3.化剂上丁二酸酐选择性达99%以上,NiO Al2O3.SiO2催化剂上两种产物都有。XRD,TPR等体相结构和表面结构的表征说明,三种催化剂的相组成、NiO的分散性及与载体的相互作用存在明显差别,这些差别可能是造成不同选择加氢性能的原因。

关键词：顺酐选择加氢气凝胶催化剂

Uncovering the synergistic photocatalytic behavior of bimetallic molecular catalysts

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Chinese Chemical Letters 2023年第1期34卷 487-492页

作者： lin Yuan Lei Zhang {1.-Xin li Jiang {3. Jing-Jing {3. Long-Zhang Dong Dong-sheng li Shun-li li Ya-Qian Lan Jiangsu Collaborative Innovation Centre of Biomedical Functional Materials Jiangsu Key Laboratory of New Power BatteriesCollege of Chemistry and Materials ScienceNanjing Normal UniversityNanjing 210023China school of Chemistry South China Normal UniversityGuangzhou 510006China school of Chemistry and Chemical {1. Southeast UniversityNanjing 211189China Key Laboratory of Inorganic Nonmetallic Crystalline and Energy Conversion Materials China Three Gorges UniversityYichang 443002China

Bimetallic catalysts usually exhibit better performance than monometallic catalysts due to synergistic ***,there is a lack of exploring the synergistic effect on catalytic performance caused by the introduction of inactive metal *** this work,we design a molecular model system that can precisely regulate the metal site number and catalytic *** these molecular metal compounds are used as homogeneous catalysts for photocatalytic CO_(2)reduction,the dinuclear heterometallic CuNi-L2shows the highest CO_(2)-to-CO conversion,which is 2.{3.and 3.0 times higher than that of dinuclear homometallic Ni2-L2and mononuclear *** functional theory calculations demonstrate that,in CuNi-L2,the introduction of inactive CuⅡis easier to promote the photo-generated electrons transferring to the coupled active NiⅡsite to achieve the highest *** addition,this work also provides insights to design and construct more efficient bimetallic catalysts in future.

关键词： Dinuclear metal compounds Crystalline photocatalyst Synergistic effect Photocatalytic CO_(2)RR Artificial photosynthesis

偏高岭土基地聚合物透水混凝土配合比优化设计

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宁德师范学院学报（自然科学版） 2024年第3期36卷 308-317页

作者：张森龙刘杰胜张一迪段诗雪谭晓明张曼武汉轻工大学土木工程与建筑学院湖北武汉430023 武汉市海洋工程结构防护材料中试平台湖北武汉430023

通过正交试验探究水胶比、目标孔隙率、偏高岭土占比和碱当量4个因素对地聚合物透水混凝土的强度指标和透水性能指标的影响.结果表明:4个因素对地聚合物透水混凝土抗压强度和透水系数的影响趋势完全相反,目标孔隙率对抗压强度和透水系... 详细信息

通过正交试验探究水胶比、目标孔隙率、偏高岭土占比和碱当量4个因素对地聚合物透水混凝土的强度指标和透水性能指标的影响.结果表明:4个因素对地聚合物透水混凝土抗压强度和透水系数的影响趋势完全相反,目标孔隙率对抗压强度和透水系数的影响最为显著,水胶比次之;通过对抗压强度与透水系数进行拟合,发现二者呈负相关,且随着龄期增长相关性有所增大;第1.组和第1.组试验配比下的地聚合物透水混凝土28 d抗压强度分别为20.7、22.0 MPa,透水系数分别为1.1.、0.52 mm·s^(-1.,满足《透水混凝土路面技术规程》(CJJ/T1.5—2009)要求,其中,第1.组实验配比下的透水系数远超规范的最低要求0.5 mm·s^(-1.,透水性能优异.

关键词：地聚合物透水混凝土配合比偏高岭土正交试验抗压强度透水系数

Utilization of Specific Attenuation for Rainfall Estimation in Southern{1.

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Journal of Tropical Meteorology 2021年第1期27卷 48-61页

作者： ZHANG A-si HUANG Chao-ying CHEN sheng ZHANG Peng-fei HU Dong-ming {1. liu-si school of Atmospheric Sciences/Key Laboratory of Tropical Atmosphere-Ocean System Sun Yat-sen UniversityZhuhaiGuangdong 519000 China Southern Laboratory of Ocean Science and engineering ZhuhaiGuangdong 519000 China Cooperative Institute for Mesoscale Meteorological Studies University of Oklahomaand NOAA/OAR/National Severe Storms LaboratoryOKC 73072 USA Guangzhou Meteorological Observatory Guangzhou 511430 China

This study uses rain gauge observations to assess the performance of different radar estimators R(ZH),R(KDP)and R(A)in estimating precipitation based on the observations of an S-band polarimetric radar over southern{1. during a typical convective storm and an extremely severe typhoon,i.e.,Typhoon *** radar estimators were derived from observations of a local autonomous particle size and velocity(Parsivel)unit(APU)disdrometer.A key parameter,alpha(α),which is the ratio of specific attenuation A to specific differential phase KDP with three fixed values(α=0.01. dB deg^(-1.,α=0.01.5 dB deg^(-1.andα=0.03.dB deg^(-1.)was examined to test the sensitivity of the R(A)rain *** results show that:(1.All radar estimators can capture the spatio-temporal patterns of two precipitation events,R(A)withα=0.01.5 dB deg^(-1.is well correlated with gauge measurement via higher Pearson’s correlation coefficient(CC)of 0.87,lower relative bias(RB)of 1.%,and lower root mean square error(RMSE)of 1..09 mm in the convective storm while it underestimates the typhoon event with RB of 3.%;(2)R(A)withα=0.03.dB deg^(-1.shows the best statistical scores with the highest CC(0.92),lowest RB(7%)and RMSE(25.74 mm)corresponding to Typhoon Manghkut;(3.R(A)estimates are more efficient in mitigating the impact of partial beam *** results indicate thatαis remarkably influenced by the variation of drop size ***,more work is needed to establish an automated and optimizedαfor the R(A)relation during different rainfall events over different regions.

关键词： QPE S-band dual polarization weather radar southern china

大棚秋冬茬紫芽青萝卜农艺性状、光合参数及可溶性糖比较分析

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江西农业学报 2024年第6期36卷 21-26页

作者：孙悦华陈月珍陈晨危咏菊孟歌孙旭李亚鹏刘佩卓张旭苏小俊王广龙熊爱生南京农业大学园艺学院作物遗传与种质创新利用全国重点实验室/农业农村部华东地区园艺作物生物学与种质创制重点实验室江苏南京210095 淮安市蔬菜科学研究所江苏淮安223021 江苏省农业科学院蔬菜研究所/江苏省高效园艺作物遗传改良重点实验室江苏南京210014 淮阴工学院生命科学与食品工程学院江苏淮安223003

为评估大棚栽培条件下8个淮科青萝卜品系(编号1.8号)的生长状况,测定了各品系紫芽青萝卜的主要农艺性状、光合参数及其可溶性糖含量,并对各项指标进行差异显著性及相关性分析,以期筛选出综合性状较为优良的紫芽青萝卜品种。结果表明:参... 详细信息

为评估大棚栽培条件下8个淮科青萝卜品系(编号1.8号)的生长状况,测定了各品系紫芽青萝卜的主要农艺性状、光合参数及其可溶性糖含量,并对各项指标进行差异显著性及相关性分析,以期筛选出综合性状较为优良的紫芽青萝卜品种。结果表明:参试的紫芽青萝卜肉质根重范围为1.7.63.286.65 g,其中3.最重,同时3.根重/叶重为5.01.显著高于其他品系;在净光合速率、蒸腾速率和气孔导度3.光合参数中,1.显著高于其他品系,其次为3.和8号;3.6号的可溶性糖含量较高,分别为225.44 mg/g和227.1. mg/g;相关性分析表明净光合速率、蒸腾速率、气孔导度以及可溶性糖含量与肉质根重指标密切相关。综上,筛选出1.3.8号作为高产型紫芽青萝卜种质资源,3.6号作为高品质型紫芽青萝卜种质资源。

关键词：紫芽青萝卜光合参数淮安地区肉质根可溶性糖种质资源