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Testing conditional independence with data missing at random

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Applied Mathematics(A Journal of Chinese Universities) 2018年第3期33卷 298-312页

作者： liu Yi liu xiao-hui College of Science China University of Petroleum (East China) School of Statistics Jiangxi University of Finance and Economics Research Center of Applied Statistics Jiangxi University of Finance and Economics

It is known that conditional independence is a quite basic assumption in many fields of statistics. How to test its validity is of great importance and has been extensively studied by the literature. Nevertheless, all of the existing methods focus on the case that data are fully observed, but none of them seems having taken into account of the scenario when missing data are present. Motivated by this, this paper develops two testing statistics to handle such a situation relying on the idea of inverse probability weighted and augmented inverse probability weighted techniques. The asymptotic distributions of the proposed statistics are also derived under the null hypothesis. The simulation studies indicate that both testing statistics perform well in terms of size and power.

关键词： conditional independence cumulative sum process of residuals missing at random inverse probability weighting re-sampling

Protein Phosphatase 2C of Toxoplasma Gondii Interacts with Human SSRP1 and Negatively Regulates Cell Apoptosis

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Biomedical and Environmental Sciences 2014年第11期27卷 883-893页

作者： GAO Xue Juan FENG Jun Xia ZHU Sen liu xiao hui TARDIEUX Isabelle liu Lang Xia Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health EngineeringJinan University Institut Cochin Université DescartesTeam FRM ‘Barriers and Pathogens’22 Rue Méchain

Objective The protozoan Toxoplasma gondii expresses large amounts of a 37 kDa Type 2C serine-threonine phosphatase,the so-called TgPP2 C which has been suggested to contribute to parasite growth *** expression in mammalian cells also indicated that the enzyme could regulate growth and *** this study,we aimed to investigate the interaction of TgPP2 C with human SSRP1（structure-specific recognition protein 1） and the effects of TgPP2 C on cell *** The yeast two hybrid system,His-tag pull-down and co-immunoprecipitation assays were used to confirm the interaction of TgPP2 C with SSRP1 and determine the binding domain on *** evaluation of cell apoptosis was performed using cleaved caspase-3 antibody and Annexin-V/PI kit combined with flow *** We identified human SSRP1 as an interacting partner of TgPP2 *** C-terminal region of SSRP1 including the amino acids 471 to 538 was specifically mapped as the region responsible for interaction with TgPP2 *** overexpression of TgPP2 C down-regulated cell apoptosis and negatively regulated apoptosis induced by DRB,casein kinase II（CKII） inhibitor,through enhanced interaction with *** TgPP2 C may be a parasitic factor capable of promoting cell survival through interaction with the host protein SSRP1,thereby creating a favorable environment for parasite growth.

关键词： Apoptosis SSRP1 TgPP2C Toxoplasma gondii

Ideal Cardiovascular Health Metrics and Coronary Artery Calcification in Northern Chinese Population:A Cross-sectional Study

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Biomedical and Environmental Sciences 2016年第7期29卷 475-483页

作者： LUO Tai Yang liu xiao hui DAI Tian Yi liu Xin Min ZHANG Qian DONG Jian Zeng Department of Cardiology Beijing An Zhen HospitalCapital Medical UniversityBeijing Institute of HeartLung and Blood Vascular Diseases

Objective Coronary artery calcification（CAC） is a well-established risk predictor of coronary heart disease events and is recognized as an indicator of subclinical *** A cross-sectional study consisting of 2999 participants aged ≥40 years from the Jidong community of Tangshan City,an industrial and modern city of China,was conducted between 2013 and 2014 to examine the association between the ideal cardiovascular health（CVH） metrics and *** ideal CVH metrics were determined based on the definition of the American Heart Association（AHA）.The participants were then grouped into 4 categories according to the quartiles of their CVH metric scores as follows：first quartile（0-2）,second quartile（3）,third quartile（4）,and fourth quartile（5-7）.CAC was assessed by using high-pitch dual-source CT,and patients were identified based on thresholds of 0,10,100,or 400 Agatston units,as per common *** The prevalence of subclinical atherosclerosis was 15.92%,13.85%,6.76%,and 1.93%,determined by using the CAC scores at thresholds of 0,10,100,and 400 Agatston units,*** with the group in the first quartile,the other three CVH groups had a lower odds ratio of CAC 0 after adjusting for age,sex,income level,education level,and alcohol use in the logistic regression *** odds ratios in these groups were 0.86 [95% confidence interval（CI）,0.63-1.17;P0.05],0.75（95% CI,0.55-1.02;P0.05）,and 0.49（95% CI,0.35-0.69;P0.05）,*** associations of CAC with the CVH metrics were consistent when different CAC cutoff scores were used（0,10,100,or 400）.Conclusion The participants with more-ideal cardiovascular metrics had a lower prevalence of subclinical atherosclerosis determined according to CAC *** an ideal cardiovascular health may be valuable in the prevention of atherosclerosis in the general population.

关键词： Ideal cardiovascular health Coronary artery calcification Atherosclerosis

ERK1/2-mediated Cytoplasmic Accumulation of hnRNPK Antagonizes TRAIL-induced Apoptosis through Upregulation of XIAP in H1299 Cells

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Biomedical and Environmental Sciences 2017年第7期30卷 473-481页

作者： HUANG Wen Si XU Feng Mei ZENG Qing Zhong liu xiao hui GAO Xue Juan liu Lang Xia Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health Engineering Jinan University Guangzhou 510632 Guangdong China

Objective Tumor necrosis factor-related apoptosis-inducing ligand （TRAIL） resistance greatly limits the clinical therapeutic efficacy of TRAIL. Elucidating the molecular mechanism underlying TRAIL resistance will be fundamental to resolving this problem. Methods Nuclear and cytoplasmic protein extraction and immunofluorescence （IF） assay were used to detect changes in heterogeneous nuclear ribonucleoprotein K （hnRNPK） localization in H1299 cells. The evaluation of cell apoptosis in cells transfected with GFP-hnRNPK, GFP-hnRNPK S284/353A, or GFP-hnRNPK S284/353D mutant was performed using cleaved caspase-3 antibody. The gene expression of XIAP was tested by quantitative RT-PCR. Results Previously, we reported that hnRNPK antagonized TRAIL-induced apoptosis through inhibition of PKC-mediated GSK313 phosphorylation. In this study, we further demonstrate that TRAIL treatment induces cytoplasmic accumulation of hnRNPK in H1299 cells. The hnRNPK localized in the cytoplasm has a higher capacity to antagonize TRAIL-induced apoptosis. Both ERK1/2 signaling inhibitor U0126 and ERK-phosphoacceptor-site mutant （GFP-hnRNPK S284/353A） diminish cytoplasmic accumulation of hnRNPK induced by TRAIL. Moreover, we show that XlAP is involved in hnRNPK-mediated TRAIL resistance in H2299 cells. Conclusion Taken together, these results give new insights into the understanding of the molecular mechanism associated with TRAIL resistance in lung adenocarcinoma.

关键词： Apoptosis ERK1/2 hnRNPK TRAIL

YWHAZ Binds to TRIM21 but Is Not Involved in TRIM21-stimulated Osteosarcoma Cell Proliferation

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Biomedical and Environmental Sciences 2018年第3期31卷 186-196页

作者： ZENG Qing Zhong liu Wan Ting LU Jun Lei liu xiao hui ZHANG Yun Fang liu Lang Xia GAO Xue Juan Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes Institute of Life and Health EngineeringJinan University Center of Kidney Disease Huadu District People's HospitalSouthern Medical University

Objective Osteosarcoma is the most common type of malignant bone tumor in children and adolescents. The role of E3 ligases in tumorigenesis is currently a focus in tumor research. In the present study, we investigated the role of the E3 ligase tripartite motif 21（TRIM21） in osteosarcoma cell *** 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenyltetrazolium bromide（MTT） assays were used to assess osteosarcoma cell viability. U2-OS cells stably carrying a recombinant lentivirus expressing tetracycline-regulated TRIM21 were screened. Co-immunoprecipitation was coupled with LCMS/MS analysis to identify novel interacting partners of TRIM21. Co-immunoprecipitation and bimolecular fluorescence complementation（BIFC） were performed to validate the interactions between TRIM21 and its novel partner YWHAZ. A TRIM21-ΔRING construct was generated to test the effects of TRIM21 ligase activity on *** TRIM21 positively regulated osteosarcoma cell proliferation. Overexpression of TRIM21 enhanced osteosarcoma cell tolerance toward various stresses. YWHAZ protein was identified as a novel interacting partner of TRIM21 and its expression levels were negatively regulated by TRIM21. The RING domain of TRIM21 was required for TRIM21 negative regulation of YWHAZ expression. However,overexpression of YWHAZ did not affect positive regulation of osteosarcoma cell proliferation by *** Our results further clarify the molecular mechanisms underlying the pathogenesis of osteosarcoma.

关键词： TRIM21 YWHAZ Osteosarcoma Proliferation

Research Progress of Acupuncture Analgesia for Labor

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Journal of Acupuncture and Tuina Science 2013年第1期11卷 57-60页

作者： liu xiao-hui Wu Ling-ling Yi Wei Huang Guo-qi Guangzhou University of Traditional Chinese Medicine Third Hospital Affiliated to Sun Yat-sen University

Labor pain is a kind of severe pain the lying-in women of natural childbirth must undergo. The labor quality is decided by the tolerance to pain. Acupuncture analgesia for labor pain has been increasingly concerned and applied by more and more clinical workers because of its safety and efficacy. By summarizing and analyzing the choice and applications of various factors of acupuncture analgesia for labor pain such as acupoint selection, instrument analgesic coefficient, and the needling techniques in acupuncture analgesia for labor pain in the recent 12 years, this study aims to provide a theoretical foundation for the clinical application of acupuncture analgesia for labor pain.

关键词： Acupuncture Therapy Electroacupuncture Acupuncture Analgesia Labor Pain Review

脂肪间充质干细胞移植治疗1型糖尿病模型大鼠的作用

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中国组织工程研究 2016年第41期20卷 6171-6176页

作者：刘小会天津市第四中心医院内分泌科天津市300140

背景:干细胞移植是1型糖尿病比较有前景的治疗方法,脂肪间充质干细胞是继骨髓间充质干细胞后的又一研究热点。目的:观察脂肪间充质干细胞移植对1型糖尿病大鼠的治疗作用。方法:将45只SD大鼠随机分为正常组、模型组和细胞移植组,后2组采... 详细信息

背景:干细胞移植是1型糖尿病比较有前景的治疗方法,脂肪间充质干细胞是继骨髓间充质干细胞后的又一研究热点。目的:观察脂肪间充质干细胞移植对1型糖尿病大鼠的治疗作用。方法:将45只SD大鼠随机分为正常组、模型组和细胞移植组,后2组采用链脲菌素腹腔注射建立1型糖尿病模型。造模后7 d,正常组腹腔静脉注射生理盐水,模型组腹腔静脉注射无血清的DMEM培养液,细胞移植组腹腔静脉注射脂肪间充质干细胞悬液。移植后2周,监测各组大鼠体质量、血糖水平,ELISA法检测各组大鼠胰岛素分泌量,RT-PCR检测胰腺组织PDX-1 m RNA的表达。结果与结论:(1)移植前细胞移植组与模型组的体质量均低于正常组,移植后2周细胞移植组大鼠体质量逐渐增加,而模型组的体质量持续下降;(2)与正常组比较,模型组大鼠空腹血糖维持在较高水平(P<0.05),与模型组相比,细胞移植组大鼠空腹血糖水平显著下降(P<0.05);(3)与正常组比较,模型组胰岛素水平明显降低(P<0.05),与模型组比较,细胞移植组胰岛素水平则显著增加(P<0.05);(4)正常组胰腺组织PDX-1 mR NA表达最高,模型组最低,各组间比较差异有显著性意义(P<0.05);(5)结果表明,脂肪间充质干细胞移植促进胰岛组织PDX-1表达,改善了大鼠的高血糖状态。

关键词：干细胞移植脂肪间充质干细胞大鼠 1型糖尿病 PDX-1 血糖胰岛素

Preliminary molecular basis of Danggui-buxue-tang on Qi deficiency and blood stasis syndrome

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沈阳药科大学学报 2008年第S1期25卷 110-111页

作者： liu Ya,LI xiao-hui(Institute of Materia Medica,College of Pharmacy,Third Military Medical University,Chongqing 400038,China)

Objective To study the molecular biology mechanism of Danggui-buxue-tang on tonifying Qi and controlling blood circulation through modulation the immune functional *** Rats were randomly divided into control group,model group,and Danggui-buxue-tang *** of Dang-guibuxue-tang on mRNA expressions of IL-1β,TNF-α,HSP-70,NF-κB,p38MAPK and JNK in blood cells of rats with Qi deficiency and blood stasis were measured with real-time fluorescent quantitative-PCR at 5th,14th and 28th *** that in artery wall were determined at the 28th ***-κB/p65 and p-c-jun protein expressions in rat artery wall were detected by western blotting as *** In model group,TNF-α,IL-1β,NF-κB,HSP-70,p38MAPK and JNK mRNA expression in blood cell increased significantly compared with control *** with model group,mRNA expression of IL-1βand JNK decreased significantly;TNF-α decreased at 5th,14th day;HSP-70,p38MAPK decreased at 14th,28th day;NF-κB decreased at 28th *** model group,TNF-α,IL-1β,NF-κB,p38MAPK and JNK mRNA expression in artery increased compared with control group,excluded HSP-70,and that in Danggui-buxue-tang group decreased *** model group,NF-κB/p65 and p-c-jun protein expression in artery increased compared with control group,and that in Danggui-buxue-tang group decreased *** The effects of Dang-guibuxue-tang on Qi deficiency and blood stasis syndrome was brought from the regulating of cytokine network at multi-link and multi-target,NF-κB,p38MAPK and JNK signal transduction pathways *** which the immune system and whole body reached to a functional balance status.

关键词： qi deficiency and blood stasis dang-guibuxue-tang cytokine network immunity signal transduction pathway

LNG-HP-β-CD包合物制备、表征及其紫外稳定性研究

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光谱学与光谱分析 2011年第3期31卷 808-811页

作者：王大伟刘琪刘明刘晓辉中国农业科学院植物保护研究所杂草鼠害生物学与治理院重点开放实验室北京100193 中国科学院动物研究所农业虫害鼠害综合治理研究国家重点实验室北京100101 中国农业科学院农业环境与可持续发展研究所北京100081 农业部旱作节水农业重点开放实验室北京100081

左炔诺孕酮(LNG)不溶于水和紫外光照易分解的特点,限制了其在鼠类不育剂中的应用。该研究采用溶液法,制备出LNG的羟丙基-β-环糊精(HP-β-CD)包合物,建立了准确简便的紫外光谱定量检测方法;通过相溶解度法确定了HP-β-CD与LNG的包合摩... 详细信息

左炔诺孕酮(LNG)不溶于水和紫外光照易分解的特点,限制了其在鼠类不育剂中的应用。该研究采用溶液法,制备出LNG的羟丙基-β-环糊精(HP-β-CD)包合物,建立了准确简便的紫外光谱定量检测方法;通过相溶解度法确定了HP-β-CD与LNG的包合摩尔比为1:1,25℃时LNG-HP-β-CD包合物的稳定常数为***-1;通过紫外光谱、傅里叶变换红外光谱表征,验证了包合物的形成;对包合物的抗紫外光降解性能进行了测试,表明在50h内包合物的降解率小于5%,远低于药物单体。结果表明,HP-β-CD对LNG具有良好的包合作用,可显著提高该药物的水溶性和紫外稳定性。

关键词：羟丙基-β-环糊精左炔诺孕酮包合物紫外吸收光谱紫外光降解