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Postulation of seedling leaf rust resistance genes in 84 Chinese winter wheat cultivars

Journal of Integrative Agriculture 2015年第10期14卷 1992-2001页

作者： REN Xiao-li liu tai-guo liu Bo GAO Li CHEN Wan-quan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Wheat leaf rust（caused by Puccinia triticina） is one of the most important fungal diseases in China. There are tens of winter wheat cultivars which are approved to be released by the government at a national level and more than 100 wheat cultivars at the provincial level. But there is no information about leaf rust（Lr） genes in these cultivars, which makes it difficult for farmers and breeders to select which cultivars they should plant in their fields and use in their breeding programs. The objective of this paper was to identify the leaf rust resistant genes at seedling stage present in the 84 commercial wheat cultivars from China that have been released in the past few years. A set of 20 near isogenic lines with Thatcher background and 6 lines with known Lr genes were used to test the virulence of 12 races of P. triticina（Pt）. By comparing the infection types（ITs） produced on the 84 cultivars by the 12 Pt races with the ITs on the differential sets, the Lr genes were postulated. In addition, 8 molecular markers of Lr genes such as Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr26 and Lr29, which are closely linked to or co-segregated with the Lr gene, were used for further validation of the genes in the 84 Chinese winter wheat cultivars. Twelve Lr genes, including Lr1, Lr3,（Lr3bg）,（Lr3ka）, Lr11, Lr13, Lr14 a, Lr16, Lr26, Lr27, Lr30 and Lr31 were postulated to be present either singly or in combinations in these Chinese wheat cultivars. Lr3 and Lr26 were detected most often in the tested cultivars, with frequencies of 51.2 and 38.1%, respectively. No wheat Lr genes were detected in 16 cultivars, and 4 cultivars may carry unknown Lr genes other than those used in this study. Lr9, Lr20, Lr21, Lr24, Lr25 and Lr29 were not present in any of the 84 tested accessions.

关键词： gene postulation molecular marker Puccinia triticina wheat leaf rust

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A FIASCO-Based Approach for Detection and Diagnosis of Puccinia graminis *** in China

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Journal of Integrative Agriculture 2014年第11期13卷 2438-2444页

作者： liu tai-guo WANG Xi GAO Li liu Bo CHEN Wan-quan XIANG Wen-sheng State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences College of Life Science Northeast Agricultural University

Stem or black rust of wheat, caused by the fungus Puccinia graminis Pers. f. sp. tritici Eriks.＆E. Henn. （Pgt）, has historically caused severe losses to wheat （Triticum aestivum L.） production worldwide. In the Fujian and Guangdong provinces of China, six moderate-to-severe epidemics of wheat stem rust have occurred, which caused destructive losses of wheat between 1949 and 1966, although these were brought under control by integrated management. A rapid and reliable detection of the pathogen will contribute to the accurate forecast and seasonal control of this disease. The objective of this study was to develop a diagnostic molecular marker generated from simple sequence repeats （SSR） for the early rapid identiifcation of P. graminis. The genomic DNA of P. graminis, Puccinia striiformis, Puccinia triticina and seven other species was ampliifed by a pair of SSR primers generated by the FIASCO （fast isolation by AFLP sequences containing repeats） enrichment protocol. The primer set Pgtw （f）/Pgtw （r） generated a polymorphic pattern displaying a 330-bp DNA fragment speciifc for P. graminis whereas no DNA fragment was obtained from other non-target wheat fungal pathogens. The detection limit of the primer was 1 ng DNA in a 25-mL PCR reaction. The SSR markers of P. graminis can also be used to detect the presence of latent hyphae in Pgt-infected wheat leaves as early as 30 h post-inoculation. A rapid approach to distinguish P. graminis from similar pathogenic fungi would be anticipated in further study.

关键词： wheat stem rust Puccinia graminis f. sp. tritci microsatellite FIASCO PCR detection

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Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences

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Journal of Integrative Agriculture 2018年第8期17卷 1779-1789页

作者： liu tai-guo GE Run-jing MA Yu-tong liu Bo GAO Li CHEN Wan-quan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Puccinia triticina, the causal agent of wheat leaf rust, is one of the most devastating rust fungi attacking wheat worldwide. Seventy-six isolates of the wheat leaf rust pathogen from Yunnan, Sichuan, Gansu and Henan provinces, China, were tested on wheat leaf rust differentials and the population structure was analyzed using four presumably neutral partial sequence markers such as elongation factor-1α(EF-1α), glyceraldehyde-3-phosphate dehydrogenase(GAPDH), β-tubulin(TUB) and the second largest RNA polymerase subunit(RPB2). The phenotypic diversity of Yunnan and Sichuan populations was higher than that of Gansu and Henan populations. The four populations were separated into two clusters based on the pathogenic data. A total of 12 single nucleotide polymorphisms(SNPs) and 32 haplotypes were identified among the four sequences. The 32 haplotypes were divided into two clusters in a neighbor-joining tree. Bayesian analyses also identified two clusters. Pairwise FST between populations in different regions were significantly different(P<0.05). Analysis of molecular variance(AMOVA) indicated that 68% of the total genetic variation was within populations.

关键词： population structure polymorphism virulence wheat leaf rust Puccinia triticina

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Jellyfish Green Fluorescent Protein(GFP) as a Reporter for Fusarium gramminearum Development on Wheat

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Journal of Integrative Agriculture 2014年第10期13卷 2177-2183页

作者： QI Jun-xian liu tai-guo XU Ying CHEN Huai-gu GAO Li liu Bo CHEN Wan-quan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Institute of Plant Protection Jiangsu Academy of Agricultural Sciences

The plasmid pGPDGFP under the control ofpgpdA promotor was used together with vector pAN7-1 containing the hygromycin resistance cassette to co-transform protoplasts of HG1, Fusarium graminearum from Hubei Province, China. Twelve out of 14 hygromycin-resistant transformants showed green signal under the UV light and contained one or several copies ofgfp, as indicated by Southern analysis of genomic DNA digested with different restriction enzymes and hybridized to the gfp probe. A single gfp copy transformant （HG1C5） was selected for further evaluation of 80 Chinese wheat cultivars or advanced lines. The results showed different resistance type to F. graminearum were observed. GFP signals observed in the rachis and adjacent spikes of 70 Chinese wheat lines such as Chuanchongzu 104 indicated both type I （host resistance to the initial infection by the fungus） and type II （resistance to the spread of FHB symptoms within an infected spike） were not observed. While other 10 lines showed type II resistance to F. graminearum with GFP signals only in inoculated spikelets. Development of the mycelium can be intuitively observed and the resistance of wheat to F. graminearum can be identified at 7 days post inoculation （dpi） in this way. The results showed no differences were evaluated between the transformed HG1C5 and the non-transgene artificial inoculation by SAS paired chi-square test and McNernar＇s test （P=-0.0625）.

关键词： Fusarium head blight (FHB) green fluorescent protein (GFP) resistance evaluation

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DIGNN-A:Real-Time Network Intrusion Detection with Integrated Neural Networks Based on Dynamic Graph

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Computers, Materials & Continua 2025年第1期82卷 817-842页

作者： Jizhao liu Minghao guo College of Computer Zhongyuan University of TechnologyZhengzhou450007China

The increasing popularity of the Internet and the widespread use of information technology have led to a rise in the number and sophistication of network attacks and security *** detection systems are crucial to network security,playing a pivotal role in safeguarding networks from potential ***,in the context of an evolving landscape of sophisticated and elusive attacks,existing intrusion detection methodologies often overlook critical aspects such as changes in network topology over time and interactions between *** address these issues,this paper proposes a real-time network intrusion detection method based on graph neural *** proposedmethod leverages the advantages of graph neural networks and employs a straightforward graph construction method to represent network traffic as dynamic graph-structured ***,a graph convolution operation with a multi-head attention mechanism is utilized to enhance the model’s ability to capture the intricate relationships within the graph structure ***,it uses an integrated graph neural network to address dynamic graphs’structural and topological changes at different time points and the challenges of edge embedding in intrusion detection *** edge classification problem is effectively transformed into node classification by employing a line graph data representation,which facilitates fine-grained intrusion detection tasks on dynamic graph node feature *** efficacy of the proposed method is evaluated using two commonly used intrusion detection datasets,UNSW-NB15 and NF-ToN-IoT-v2,and results are compared with previous studies in this *** experimental results demonstrate that our proposed method achieves 99.3%and 99.96%accuracy on the two datasets,respectively,and outperforms the benchmark model in several evaluation metrics.

关键词： Intrusion detection graph neural networks attention mechanisms line graphs dynamic graph neural networks

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The complex effects of miR-146a in the pathogenesis of Alzheimer's disease

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Neural Regeneration Research 2025年第5期20卷 1309-1323页

作者： Yunfan Long Jiajia liu Yu Wang Haidong guo guohong Cui Department of Neurology Shanghai No.9 People’s HospitalShanghai Jiaotong University School of MedicineShanghaiChina Academy of Integrative Medicine Shanghai University of Traditional Chinese MedicineShanghaiChina School of Integrative Medicine Shanghai University of Traditional Chinese MedicineShanghaiChina Department of Neurology Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese MedicineShanghaiChina

Alzheimer's disease is a neurodegenerative disorder characterized by cognitive dysfunction and behavioral *** plaques formed through the extracellular deposition of amyloid-βproteins,as well as neurofibrillary tangles formed by the intracellular deposition of hyperphosphorylated tau proteins,comprise two typical pathological features of Alzheimer's *** symptomatic treatment,there are no effective therapies for delaying Alzheimer's disease ***(miR)are small,non-coding RNAs that negatively regulate gene expression at the transcriptional and translational levels and play important roles in multiple physiological and pathological ***,miR-146a,a NF-κB-regulated gene,has been extensively implicated in the development of Alzheimer's disease through several *** has demonstrated substantial dysregulation of miR-146a both during the initial phases and throughout the progression of this *** R-146a is believed to reduce amyloid-βdeposition and tau protein hyperphosphorylation through the TLR/IRAK1/TRAF6 pathway;however,there is also evidence supporting that it can promote these processes through many other pathways,thus exacerbating the pathological manifestations of Alzheimer's *** has been widely reported that miR-146a mediates synaptic dysfunction,mitochondrial dysfunction,and neuronal death by targeting m RNAs encoding synapticrelated proteins,mitochondrial-related proteins,and membrane proteins,as well as other *** the impact on glial cells,miR-146a also exhibits differential *** one hand,it causes widespread and sustained inflammation through certain pathways,while on the other hand,it can reverse the polarization of astrocytes and microglia,alleviate neuroinflammation,and promote oligodendrocyte progenitor cell differentiation,thus maintaining the normal function of the myelin sheath and exerting a protective effect on *** this review,we provide a

关键词： Alzheimer's disease amyloid-β glial cells microRNAs miR-146a neuroinflammatory

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An investigation on the wind profiles and gravity wave dynamics in MLT region based on the meteor radars from the Meridian Project

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Earth and Planetary Physics 2025年第1期9卷 29-38页

作者： tai liu Zhe Wang MengXi Shi Willie Soon ShiCan Qiu Department of Geophysics College of the Geology Engineering and GeomaticsChang’an UniversityXi’an 710054China Center for Environmental Research and Earth Sciences(CERES) SalemMA 01970USA Institute of Earth Physics and Space Science(ELKH EPSS) 9400SopronHungary

The meteor radar can detect the zenith angle,azimuth,radial velocity,and altitude of meteor trails so that one can invert the wind profiles in the mesosphere and low thermosphere(MLT)region,based on the Interferometric and Doppler *** this paper,the horizontal wind field,gravity wave(GW)disturbance variance,and GW fluxes are analyzed through the meteor radar observation from 2012−2022,at Mohe(53.5°N,122.4°E)and Zuoling(30.5°N,114.6°E)stations of the(Chinese)Meridian *** Lomb−Scargle periodogram method has been utilized to analyze the periodic variations for time series with observational data *** results show that the zonal winds at both stations are eastward dominated,while the meridional winds are southward *** variance of GW disturbances in the zonal and meridional directions increases gradually with height,and there is a strong pattern of annual *** zonal momentum flux of GW changes little with height,showing weak annual *** meridional GW flux varies gradually from northward to southward with height,and the annual periodicity is *** both stations,the maximum values of zonal and meridional wind occur close to the peak heights of GW flux,with opposite *** observational evidence is consistent with the filtering *** horizontal wind velocity,GW flux,and disturbance variance of the GW at Mohe are overall smaller than those at Zuoling,indicating weaker activities in the MLT at *** power spectral density(PSD)calculated by the Lomb−Scargle periodogram shows that there are 12-month period and 6-month period in horizontal wind field,GW disturbance variance and GW flux at both stations,and especially there is also a 4-month cycle in the disturbance *** PSD of the 12-month and 6-month cycles exhibits maximum values below 88 km and above 94 km.

关键词： meteor radar wind field gravity wave Lomb−Scargle method

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永古高速公路SBS改性沥青试验研究

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硅酸盐通报 2014年第8期33卷 1956-1961页

作者：杨成刘德仁王旭刘国太兰州交通大学土木工程学院兰州730070 西安铁路局汉中工务段汉中723000

本试验主要研究目的是SBS改性剂、相容剂、稳定剂、剪切速率、剪切时间、发育时间对永古高速公路SBS改性沥青性能的影响。查阅该地区沥青路面资料,初步确定在相容剂掺量1.5%,稳定剂掺量1.5‰的前提下,通过对沥青软化点、25℃的针入度、... 详细信息

本试验主要研究目的是SBS改性剂、相容剂、稳定剂、剪切速率、剪切时间、发育时间对永古高速公路SBS改性沥青性能的影响。查阅该地区沥青路面资料,初步确定在相容剂掺量1.5%,稳定剂掺量1.5‰的前提下,通过对沥青软化点、25℃的针入度、5℃的延度、离析软化点差进行试验,对试验结果进行分析,最终确定SBS改性沥青的SBS改性剂掺量3.8%、相容剂掺量1.5%、稳定剂掺量2‰。在此SBS改性沥青的前提下,研究剪切速率、剪切时间、发育时间对SBS改性沥青的影响,在剪切时间30 min,发育时间90 min的前提下,继续对沥青软化点、25℃的针入度、5℃的延度进行试验,整理分析数据,得到最终的制备工艺为:剪切速率5500 r·min-1、剪切时间35 min、发育时间为120 min。得到结论为永古高速SBS改性沥青为:SBS改性剂掺量3.8%、相容剂掺量1.5%、稳定剂掺量2‰、剪切速率5500 r·min-1、剪切时间35 min、发育时间120 min。

关键词：改性沥青 SBS改性剂相容剂稳定剂生产工艺

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Bonifiber纤维对沥青混合料抗疲劳性能的影响

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硅酸盐通报 2014年第4期33卷 811-815页

作者：杨成刘德仁王旭刘国太兰州交通大学土木工程学院兰州730070

本文采用MTS-810材料测试系统,对AC-20及SMA-13两种沥青混合料进行疲劳测试,标本总共96份,分别采用0.3 MPa、0.4 MPa、0.5 MPa、0.6 MPa、0.8 MPa、1.0 MPa、1.2 MPa、1.6 MPa的常应力、三分点加载,对AC-20、AC-20+0.2%Bonifiber与SMA-13、SMA-13+0.1%Bonifiber分别做疲劳试验对比,每组24份样本,每种常应力下做3份样本,由实验结果可知,加入Bonifiber纤维的沥青混合料强度和刚度明显提高,拟合四组疲劳试验结果,得到的线性回归。尤其对SMA-13,抗弯刚度增长了142%,极限荷载提高了38%。在允许应力范围内,掺入Bonifiber纤维的AC-20,随应力的增长疲劳寿命的变化率也在增长。掺入Bonifiber纤维的SMA-13,随应力的增长疲劳寿命变化率反而在减小。

关键词： Bonifiber 沥青混合料疲劳试验 AC-20 SMA-13

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上海市杨浦区街旁绿地景观调查

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安徽农业科学 2016年第22期44卷 156-159页

作者：刘国泰上海交通大学上海200240

城市街旁绿地是城市绿地系统中的一部分,通常沿街而设,面积较小,使用频率高,具有改善生态环境,美化城市景观,为市民提供游憩场地的功能。随着经济发展及生活水平的提高,城市中街旁绿地的规划、设计和建设需进一步改善和提高以满足人们... 详细信息

城市街旁绿地是城市绿地系统中的一部分,通常沿街而设,面积较小,使用频率高,具有改善生态环境,美化城市景观,为市民提供游憩场地的功能。随着经济发展及生活水平的提高,城市中街旁绿地的规划、设计和建设需进一步改善和提高以满足人们的需求。该研究通过对上海市杨浦区5个具有代表性的街旁绿地景观进行调研,从基本情况、空间设计、景观要素等方面来比较分析,欲了解目前上海市街旁绿地的基本现状,发现街旁绿地规划设计方面存在的问题,并提出一些可行性建议,为今后上海市街旁绿地的景观规划设计提供参考。

关键词：街旁绿地空间设计景观要素

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