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Overexpression of a Novel Gene r18(t) Reduces the Expression Level of IAA Related Genes and Induces Abaxial Curling of Leaf Blades in Rice

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Overexpression of a Novel Gene r18(t) Reduces the Expression...

第十二届全国植物基因组学大会

作者： Ju Gao,Xuejian Shi,Wenyi Wang,Haibing zhao,Zemin Zhang~* college of Agriculture,south china agricultural university,guangzhou 510642.china

Leaf is one of the most important organ of a plant,which is in charge of accomplishing photosynthesis, respiration and *** we report a novel gene r18(t),overexpression of which make the leaf rolled abxial in *** homozygous mutant r18(t),isolated from T-DNA insertion line(Constructed by SIPPE), displays a phenotype with serious curling and narrow leaf compared to wild type *** of T-DNA border sequence shows that T-NDA didn’t direct insert in a known gene,and affymetrix genechip as-

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OsRAP,a Novel Nuclear-encoded Protein,Is Essential For Chloroplast Development in Rice

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OsRAP,a Novel Nuclear-encoded Protein,Is Essential For Chlor...

第十二届全国植物基因组学大会

作者： Jianjie Tan,Yi Xing,Haibing zhao,Shengze Wu,Zemin Zhang~* college of Agriculture,south china agricultural university,guangzhou 510642.china

Chloroplast contains more than 3000 proteins,most of them are encoded in cytoplasm as precursors and transported into ***,chloroplast development requires lots of nuclear-encoded proteins. Here,we report a novel RAP domain(RNA-binding domain in Apicomplexans) protein in rice,which is encoded by a nuclear gene *** of T-DNA in the second exon of OsRAP leads to silencing of its expression and results in albino phenotype and seedling *** analysis demonstrate OsRAP is a single recessive mutant *** of Chlorophyll content and Transmission electron microscopy show

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Optimal combination of the correction model and parameters for the precision geometric correction of UAV hyperspectral images

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International Journal of agricultural and Biological Engineering 2024年第3期17卷 173-184页

作者： Wenzhong Tian Za Kan Qingzhan zhao Ping Jiang Xuewen Wang Hanqing liu college of Mechanical and Electrical Engineering Shihezi UniversityShihezi 832000XinjiangChina Research Center for Space information Engineering Technology Shihezi 832000XinjiangChina XPCC Division of National Remote Sensing Center of china Shihezi 832000XinjiangChina college of information Science and Technology Shihezi UniversityShihezi 832000XinjiangChina

Nowadays,with the rapid development of quantitative remote sensing represented by high-resolution UAV hyperspectral remote sensing observation technology,people have put forward higher requirements for the rapid preprocessing and geometric correction accuracy of hyperspectral *** optimal geometric correction model and parameter combination of UAV hyperspectral images need to be determined to reduce unnecessary waste of time in the preprocessing and provide high-precision data support for the application of UAV hyperspectral *** this study,the geometric correction accuracy under various geometric correction models(including affine transformation model,local triangulation model,polynomial model,direct linear transformation model,and rational function model)and resampling methods(including nearest neighbor resampling method,bilinear interpolation resampling method,and cubic convolution resampling method)were ***,the distribution,number,and accuracy of control points were analyzed based on the control variable method,and precise ground control points(GCPs)were *** results showed that the average geometric positioning error of UAV hyperspectral images(at 80 m altitude AGL)without geometric correction was as high as 3.4041 m(about 65 pixels).The optimal geometric correction model and parameter combination of the UAV hyperspectral image(at 80 m altitude AGL)used a local triangulation model,adopted a bilinear interpolation resampling method,and selected 12 edgemiddle distributed *** correction accuracy could reach 0.0493 m(less than one pixel).This study provides a reference for the geometric correction of UAV hyperspectral images.

关键词： geometric correction hyperspectral images unmanned aerial vehicle(UAV) ground control point(GCP)

The appearance of cytoplasmic cytochrome C precedes apoptosis during Drosophila salivary gland degradation

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Insect Science 2024年第1期31卷 157-172页

作者： Shihui Long Wenxin Cao Yongyu Qiu Ruohan Deng Jiali liu Lidan Zhang Renke Dong Fengxin liu Sheng Li Haigang zhao Na Li Kang Li Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhouChina Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhouGuangdong ProvinceChina School of Food Science and Engineering South China University of TechnologyGuangzhouChina ChemPartner PharmaTech Co. LtdJiangmenGuangdong ProvinceChina

Apoptosis is an important process for organism development that functions to eliminate cell damage,maintain homeostasis,and remove obsolete tissues during *** mammals,apoptosis is accompanied by the release of cytochrome C(Cyt-c)from mitochondria to the ***,whether this process is conserved in the fruit fly,Drosophila melanogaster,remains *** this study,we discovered that during the degradation of Drosophila salivary gland,the transcription of mitochondria apoptosis factors(MAPFs),Cyt-c,and death-associated APAF1-related killer(Dark)encoding genes are all upregulated antecedent to initiator and effector caspases encoding *** proteins Cyt-c and the active caspase 3 appear gradually in the cytoplasm during salivary gland ***,the Cyt-c protein colocates with mito-GFP,the marker indicating cytoplasmic mitochondria,and the change in mitochondrial membrane potential coincides with the appearance of Cyt-c in the ***,impeding or promoting 20E-induced transcription factor E93 suppresses or enhances the staining of Cyt-c and the active caspase 3 in the cytoplasm of salivary gland,and accordingly decreases or increases the mitochondrial membrane potential,*** research provides evidence that cytoplasmic Cyt-c appears before apoptosis during Drosophila salivary gland degradation,shedding light on partial conserved mechanism in apoptosis between insects and mammals.

关键词： active caspase 3 apoptosis Cyt-c Drosophila salivary gland

Highly efficient transformation of intact yeast-like conidium cells of Tremella fuciformis by electroporation

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Science china(Life Sciences) 2008年第10期51卷 932-940页

作者： GUO LiQiong1,2, liu Yong1, zhao ShuXian1, liu ErXian1 & LIN JunFang1,2.1 college of Food Sciences, south china agricultural university, guangzhou 510640, china 2 Institute of Biomass Research, south china agricultural university, guangzhou 510640, china college of Food Sciences South China Agricultural University Guangzhou 510640 China

Tremella fuciformis is one of higher basidiomycetes. Its basidiospore can reproduce yeast-like conidia, also called the blastospore by budding. The yeast-like conidia of T. fuciformis is monokaryotic and easy to culture by submerged fermentation similar to yeast. So it is a good recipient cell for exogenous gene expression. In this study, two expression vectors pGlg-gfp containing gpd-Gl promoter and gfp gene and pGlg-hph containing gpd-Gl promoter and hph gene were constructed. The lowest sensitive concentration of hygromycin for the blastospore was determined on three types of media. Our ex- periments showed that the lowest sensitive concentration of hygromycin for the blastospore was 5 μg/mL on MA medium. The intact blastospores were transformed with the expression vector pGlg-hph by electroporation. The putative transformants were obtained by the MA selective medium. Experi- mental results showed that the most effective parameters for the electroporation of intact blastospores were obtained by using STM buffer, 1.0×108 cells/mL of blastospores, 200 μL in transformation volume, 6 μg plasmid, 2.0 kV/cm of electric pulse voltage, stillness culturing on MB liquid medium for 48 h after electroporation. In these transformation conditions, the efficiency reached 277 colonies/μg DNA. Co-transformation of plasmid pGlg-gfp and pGlg-hph with ratio of 1:1 was performed by electroporation with the optimal parameters. The putative co-transformants were obtained by the MA selective medium. Eight randomly selected colonies from the vast putative co-transformants were analyzed by PCR de- tection and southern blotting. The experiments showed that the gfp was integrated into the genomes of three transformants. The co-transformation efficiency was 37.5%. Green fluorescence was observed under laser scanning confocal microscope in these gfp positive transformants. This indicates that the exogenous gfp can be expressed effectively in the yeast-like conidia of T. fuciformis.

关键词： Tremella fuciformis electroporation yeast-like conidia intact cells gene expression hph gene gfp gene

Rural Finance, Farmland Transfer and agricultural Production Technical Efficiency: Evidence from china

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Rural Finance, Farmland Transfer and Agricultural Production...

2.10 2nd IEEE International Conference on information and Financial Engineering(ICIFE2.10)

作者： Zongzhang Li college of Economics and Management south china agricultural university guangzhou china

This paper studies the roles played by rural finance and farmland transfer on agricultural production technical efficiency, using data from the second agricultural census of china. It first estimates agricultural production technical efficiency of each administrative area of china by employing Data Envelopment Analysis. A Tobit model is then applied to investigate the effects of formal credit, informal credit and farmland transfer on technical efficiency. The empirical results suggest that rural formal credit and farmland transfer both play positive role on technical efficiency, while informal credit constrains the improvement of technical efficiency.

关键词： rural finance farmland transfer technical efficiency Agricluture

Root-derived long-distance signals trigger ABA synthesis and enhance drought resistance in Arabidopsis

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Journal of Genetics and Genomics 2024年第7期51卷 749-761页

作者： Qian-Qian liu Jin-Qiu Xia Jie Wu Yi Han Gui-Quan Zhang Ping-Xia zhao Cheng-Bin Xiang Division of Life Sciences and Medicine Division of Molecular&Cell BiophysicsHefei National Science Center for Interdisciplinary Sciences at the MicroscaleUniversity of Science and Technology of ChinaThe Innovation Academy of Seed DesignChinese Academy of SciencesHefeiAnhui 230027China college of Life Sciences Anhui Agricultural UniversityHefeiAnhui 230036China college of Agronomy South China Agricultural UniversityGuangzhouGuangdong 510642China School of Biology and Basic Medical Sciences Soochow UniversitySuzhouJiangsu 215123China

Vascular plants have evolved intricate long-distance signaling mechanisms to cope with environmental stress,with reactive oxygen species(ROS)emerging as pivotal systemic signals in plant stress ***,the exact role of ROS as root-to-shoot signals in the drought response has not been *** this study,we reveal that compared with wild-type plants,ferric reductase defective 3(frd3)mutants exhibit enhanced drought resistance concomitant with elevated NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3(NCED3)transcript levels and abscisic acid(ABA)contents in leaves as well as increased hydrogen peroxide(H_(2)O_(2))levels in roots and *** experiments distinctly illustrate that drought resistance can be conferred by the frd3 rootstock regardless of the scion genotype,indicating that long-distance signals originating from frd3 roots promote an increase in ABA levels in ***,the drought resistance conferred by the frd3 mutant rootstock is weakened by the CAT2-overexpressing scion,suggesting that H_(2)O_(2)may be involved in long-distance ***,the results of comparative transcriptome and proteome analyses support the drought resistance phenotype of the frd3 *** together,our findings substantiate the notion that frd3 root-derived long-distance signals trigger ABA synthesis in leaves and enhance drought resistance,providing new evidence for root-to-shoot long-distance signaling in the drought response of plants.

关键词： frd3 Drought resistance Root-to-shoot long-distance signals Reactive oxygen species(ROS) H_(2)O_(2) Iron ABA

Identification of a Resistance Gene to Rice Blast in a Space-induced Rice Mutant

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Identification of a Resistance Gene to Rice Blast in a Space...

中国植物保护学会2009年学术年会

作者： Wuming Xiao~(1,2) Qiyun Yang~(1*) Zhiqiang Chen~(2*) Wang Hui~2 Tao Guo~2 Yongzhu liu~2 Xiaoyuan Zhu~1 (1.Plant Protection Research Institute,Guangdong Academy of agricultural Sciences, guangzhou 510640,china 2.college of Agriculture,south china agricultural university, guangzhou 510642.china)

Utilization of resistance(R) genes has been proven the most effective and economical approach to control rice *** space-induced rice mutant H4 expresses broad-spectrum resistance to most of isolates collected from Guangdong province,*** understand its resistance to rice blast, genetic and mapping analysis of the blast resistance(R) gene in H4 were *** analysis indicated that blast resistance to a representative isolate was controlled by a single dominant locus designated as Pih4(t).Highly susceptible F individuals were performed with linkage analysis,the Pih4 (t) locus was preliminarily linked to markers RM224 and RM27360 with≈1.04 cM and 1.2 cM in the long arm of chromosome 11,*** current study provides essential information for the cloning of the Pih4(t) gene and for marker-assisted selection in rice breeding for blast resistance.

关键词： Rice blast Resistance gene Gene mapping

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高校图书馆与创新教育

图书馆论坛 2004年第4期24卷 189-190,48页

作者：黄雪雄华南农业大学图书馆广东广州510642

论述了高校开展创新教育的重要性和高校图书馆完全具备创新教育的功能。简述了在创新教育中高校图书馆应采取的措施 :改革管理体制 ,促进管理创新 ;做好读者咨询和阅读指导工作 ;创造浓厚的文化气息和文明氛围 ;开展图书信息学教育 ,提... 详细信息

论述了高校开展创新教育的重要性和高校图书馆完全具备创新教育的功能。简述了在创新教育中高校图书馆应采取的措施 :改革管理体制 ,促进管理创新 ;做好读者咨询和阅读指导工作 ;创造浓厚的文化气息和文明氛围 ;开展图书信息学教育 ,提高大学生的信息素质 ;

关键词：高校图书馆创新教育管理体制阅读指导信息学教育