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Highly efficient transformation of intact yeast-like conidium cells of Tremella fuciformis by electroporation

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Science china(Life Sciences) 2008年第10期51卷 932-940页

作者： GUO LiQiong1,2, liu Yong1, zhao ShuXian1, liu ErXian1 & LIN JunFang1,2.1 college of Food Sciences, south china agricultural university, guangzhou 510640, china 2 Institute of Biomass Research, south china agricultural university, guangzhou 510640, china 1. college of Food Sciences South China Agricultural University Guangzhou 510640 China 2. Institute of Biomass Research South China Agricultural University Guangzhou 510640 China

Tremella fuciformis is one of higher basidiomycetes. Its basidiospore can reproduce yeast-like conidia, also called the blastospore by budding. The yeast-like conidia of T. fuciformis is monokaryotic and easy to culture by submerged fermentation similar to yeast. So it is a good recipient cell for exogenous gene expression. In this study, two expression vectors pGlg-gfp containing gpd-Gl promoter and gfp gene and pGlg-hph containing gpd-Gl promoter and hph gene were constructed. The lowest sensitive concentration of hygromycin for the blastospore was determined on three types of media. Our ex- periments showed that the lowest sensitive concentration of hygromycin for the blastospore was 5 μg/mL on MA medium. The intact blastospores were transformed with the expression vector pGlg-hph by electroporation. The putative transformants were obtained by the MA selective medium. Experi- mental results showed that the most effective parameters for the electroporation of intact blastospores were obtained by using STM buffer, 1.0×108 cells/mL of blastospores, 200 μL in transformation volume, 6 μg plasmid, 2.0 kV/cm of electric pulse voltage, stillness culturing on MB liquid medium for 48 h after electroporation. In these transformation conditions, the efficiency reached 277 colonies/μg DNA. Co-transformation of plasmid pGlg-gfp and pGlg-hph with ratio of 1:1 was performed by electroporation with the optimal parameters. The putative co-transformants were obtained by the MA selective medium. Eight randomly selected colonies from the vast putative co-transformants were analyzed by PCR de- tection and southern blotting. The experiments showed that the gfp was integrated into the genomes of three transformants. The co-transformation efficiency was 37.5%. Green fluorescence was observed under laser scanning confocal microscope in these gfp positive transformants. This indicates that the exogenous gfp can be expressed effectively in the yeast-like conidia of T. fuciformis.

关键词： Tremella fuciformis electroporation yeast-like conidia intact cells gene expression hph gene gfp gene

Early Cretaceous Metasomatized Lithospheric Mantle beneath the Central Jiangnan Orogen in south china:Geochemical and Sr-Nd Isotope Evidence from the Tuanshanbei Dolerite

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Acta Geologica Sinica(English Edition) 2024年第3期98卷 602-616页

作者： WANG Cheng WEI Hantao liu Qingquan YANG zhao Guangdong Provincial Key Laboratory of Geodynamics and Geohazards School of Earth Sciences and EngineeringSun Yat-sen UniversityGuangzhou 510275China Key Laboratory of Metallogenic Prediction of Nonferrous Metals and Geological Environment Monitoring Ministry of EducationSchool of Geosciences and Info-PhysicsCentral South UniversityChangsha 410083China Development and Research Centre China Geological SurveyBeijing 100037China School of Environmental Studies China University of GeosciencesWuhan 430074China

It is well established that Cretaceous magmatism in the south china Block(SCB)is related to the Paleo-Pacific ***,the starting time and the associated deep crust-mantle processes are still *** dike swarms carry important information on the deep earth(including mantle)geodynamics and geochemical *** the Jiangnan Orogen(south china).there is no information on whether the Mesozoic magmatic activities in this region are also directly related to the Pacific subduction or *** this study,we present detailed zircon U-Pb geochronological,wholerock element and Sr-Nd isotope data for Early Cretaceous Tuanshanbei dolerite dikes,and provide new constraints on the condition of the lithospheric mantle and mantle dynamics of the SCB during that ***-ICP-MS zircon U-Pb dating suggests that this dolerite erupted in the Early Cretaceous(~145 Ma).All samples have alkaline geochemical affinities with K_(2)O+Na_(2)O=3.11-4.04 wt%,K_(2)O/Na_(2)O=0.50-0.72,and Mg^(#)=*** are enriched in LILE but depleted in HFSE with higher initial^(87)Sr/^(86)Sr ratio(0.706896-0.714743)and lower ε_(Nd)(t)(-2.61 to-1.67).They have high Nb/U,Nb/La,La/Sm and Rb/Sr,and low La/Nb,La/Ta,Ce/Pb,Ba/Rb,Tb/Yb and Gd/Yb *** geochemical signatures suggest that the fractional crystallization is obvious but crustal contamination play a negligible role during magmatic *** dolerite were most likely derived from low-degree(2%-5%)partial melting of a phlogopite-bearing mantle material consisted of~85% spinel peridotite and~15% garnet peridotite previously metasomatized by asthenospherederived fluids/melts with minor subduction-derived fluids/***-rollback generally lead to the upwelling of the hot *** upwelling of asthenosphere consuming the lithospheric mantle by thermo-mechanical-chemical *** lithospheric mantle may have partially melted due to the heating by the upwelling asthenosphere and lithospheric extension.I

关键词： Early Cretaceous mafic dike metasomatized lithospheric mantle Tuanshanbei dolerite Jiangnan Orogen south china

Transmit Power Minimization for IRS-Assisted NOMA-UAV Networks

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china Communications 2024年第5期21卷 137-152页

作者： zhao Chen Pang Xiaowei Tang Jie liu Mingqian zhao Nan Zhang Xiuyin Wang Xianbin School of information and Communication Engineering Dalian University of TechnologyDalian 116024China School of Electronic and information Engineering South China University of TechnologyGuangzhou 510641China State Key Laboratory of Integrated Services Networks Xidian UniversityXi’an 710071China Department of Electrical and Computer Engineering Western UniversityLondonON N6A 5B9Canada

The flexibility of unmanned aerial vehicles(UAVs)allows them to be quickly deployed to support ground *** reflecting surface(IRS)can reflect the incident signal and form passive beamforming to enhance the signal in the specific *** by the promising benefits of both technologies,we consider a new scenario in this paper where a UAV uses non-orthogonal multiple access to serve multiple users with *** to their distance to the UAV,the users are divided into the close users and remote *** UAV hovers above the close users due to their higher rate requirement,while the IRS is deployed near the remote users to enhance their received *** aim at minimizing the transmit power of UAV by jointly optimizing the beamforming of UAV and the phase shift of IRS while ensuring the decoding ***,the problem is ***,we decompose it into two sub-problems,including the transmit beamforming optimization and phase shift optimization,which are transformed into second-order cone programming and semidefinite programming,*** propose an iterative algorithm to solve the two sub-problems *** results prove the effectiveness of the proposed scheme in minimizing the transmit power of UAV.

关键词： intelligent reflecting surface nonorthogonal multiple access phase shift optimization transmit beamforming optimization unmanned aerial vehicle

OsDA1 positively regulates grain width in rice

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The Crop Journal 2024年第1期12卷 92-101页

作者： Cong Li Jun liu Liya Zhang Tao Li Hongyu Li Bin liu Tao zhao Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Institute of Nanfan&Seed Industry Guangdong Academy of SciencesGuangzhou 510316GuangdongChina

The size and shape of rice grains influence their yield and commercial *** investigated the role of OsDA1,a rice homolog of the Arabidopsis DA1 gene,in regulating grain size and ***1 was highly expressed in young spikelets and *** overexpression led to enlarged seeds with increased width and decreased length/width ratio(LWR)and knocking out OsDA1 reduced grain width and increased grain length and LWR.A R310K point mutation in the DA1-like domain is a potential target for breeding for increased grain width and ***1 interacted with TCP gene-family proteins to regulate grain size and *** findings deepen our understanding of the molecular mechanisms underlying grain size regulation and provide useful information for improving grain yield.

关键词： Rice Osda1 Grain size Grain shape Grain length/width ratio TCP

Host-Induced Gene Silencing of Effector AGLIP1 Enhanced Resistance of Rice to Rhizoctonia solani AG1-IA

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Rice science 2024年第4期31卷 463-474,I0031-I0034页

作者： zhao Mei liu Xiaoxue WAN Jun ZHOU Erxun SHU Canwei Pesticide State Key Laboratory of Green Pesticide Guangdong Province Key Laboratory of Microbial Signals and Disease ControlCollege of Plant ProtectionSouth China Agricultural UniversityGuangzhou 510642China college of Life Science and Engineering Henan University of Urban ConstructionPingdingshan 467036China

Rice sheath blight, caused by Rhizoctonia solani AG1-IA, is a major disease in rice-growing areas worldwide. Effectors of phytopathogenic fungi play important roles during the infection process of fungal pathogens onto their host plants. However, the molecular mechanisms by which R. solani effectors regulate rice immunity are not well understood. Through prediction, 78 candidate effector molecules were identified. Using the tobacco rattle virus-host induced gene silencing(TRV-HIGS) system, 45 RNAi constructs of effector genes were infiltrated into Nicotiana benthamiana leaves. The results revealed that eight of these constructs resulted in a significant reduction in necrosis caused by infection with the AG1-IA strain GD-118. Additionally, stable rice transformants carrying the double-stranded RNA construct for one of the effector genes, AGLIP1, were generated to further verify the function of this gene. The suppression of the AGLIP1 gene increased the resistance of both N. benthamiana and rice against GD-118, and also affected the growth rate of GD-118, indicating that AGLIP1 is a key pathogenic factor. Small RNA sequencing showed that the HIGS vectors were processed into si RNAs within the plants and then translocated to the fungi, leading to the silencing of the target genes. As a result, AGLIP1 might be an excellent candidate for HIGS, thereby enhancing crop resistance against the pathogen and contributing to the control of R. solani infection.

关键词： Rhizoctonia solani host-induced gene silencing lipase tobacco rattle virus

RETINOBLASTOMA RELATED 1 switches mitosis to meiosis in rice

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Plant Communications 2024年第6期5卷 193-212页

作者： Yongjie Miao Hanli You Huixin liu Yangzi zhao Jiangzhe zhao Yafei Li Yi Shen Ding Tang Baohui liu Kewei Zhang Zhukuan Cheng Guangdong Provincial Key Laboratory of Plant Adaptation and Molecular Design Innovative Center of Molecular Genetics and EvolutionSchool of Life SciencesGuangzhou UniversityGuangzhou 510006China Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education Agricultural College of Yangzhou UniversityYangzhou 225009China State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants Department of BiologyCollege of Life SciencesZhejiang Normal UniversityJinhua 321004China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou UniversityYangzhou 225009China

The transition from mitosis to meiosis is a critical event in the reproductive development of all sexually reproducing ***,the mechanisms that regulate this process in plants remain largely ***,we find that the rice(Oryza sativa L.)protein RETINOBLASTOMA RELATED 1(RBR1)is essential to the transition from mitosis to *** of RBR1 function results in hyper-proliferative sporogenous-celllike cells(SCLs)in the anther locules during early stages of reproductive *** hyperproliferative SCLs are unable to initiate meiosis,eventually stagnating and degrading at late developmental stages to form pollen-free *** results suggest that RBR1 acts as a gatekeeper of entry into ***,cytokinin content is significantly increased in rbr1 mutants,whereas the expression of type-B response factors,particularly LEPTO1,is significantly *** the known close association of cytokinins with cell proliferation,these findings imply that hyper-proliferative germ cells in the anther locules may be attributed to elevated cytokinin concentrations and disruptions in the cytokinin *** a genetic strategy,the association between germ cell hyper-proliferation and disturbed cytokinin signaling in rbr1 has been *** summary,we reveal a unique role of RBR1 in the initiation of meiosis;our results clearly demonstrate that the RBR1 regulatory module is connected to the cytokinin signaling pathway and switches mitosis to meiosis in rice.

关键词： RETINOBLASTOMA RELATED1 meiosis initiation cytokinin rice

Rice gene OsUGT75A regulates seedling emergence under deep-sowing conditions

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The Crop Journal 2024年第1期12卷 133-141页

作者： Jia zhao Siyu liu Xiaoqian zhao Zhibo Huang Shan Sun Zixuan Zeng Yongqi He Zhoufei Wang The Laboratory of Seed Science and Technology Guangdong Key Laboratory of Plant Molecular BreedingNational Engineering Research Center of Plant Space BreedingSouth China Agricultural UniversityGuangzhou 510642GuangdongChina

Poor seedling emergence is a challenge for direct seeding of rice under deep-sowing field *** we reveal that UDP-glucosyltransferase OsUGT75A promotes rice seedling emergence under deepsowing conditions by increasing shoot *** of OsUGT75A was higher in the middle regions of the shoot and in shoots under deep-sowing *** of free abscisic acid(ABA)and jasmonates(JA)were higher in shoots of OsUGT75A mutants than in those of wild-type plants,and OsUGT75A mutants were more sensitive to ABA and JA *** shoot length was attributed to higher ABA INSENSITIVE 3(OsABI3)expression and lower JASMONATE-ZIM domain protein(OsJAZ)expression in *** extension by OsUGT75A is achieved mainly by promotion of cell *** elite haplotype of OsUGT75A associated with increased shoot length was identified among indica rice ***75A acts to increase seedling emergence under deep-sowing conditions.

关键词： Abscisic acid Deep-sowing Jasmonic acid Rice Shoot length

Adaptive neural network control of a 2-DOF helicopter system considering input constraints and global prescribed performance

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Science china(information Sciences) 2024年第7期67卷 224-239页

作者： Zhijia zhao Jiale WU Zhijie liu We HE C.L.Philip CHEN School of Mechanical and Electrical Engineering Guangzhou University School of Intelligence Science and Technology and Key Laboratory of Intelligent Bionic Unmanned Systems of Ministry of Education University of Science and Technology Beijing School of Computer Science and Engineering South China University of Technology Pazhou Lab

In this study, an adaptive neural network(NN) control is proposed for nonlinear two-degree-offreedom(2-DOF) helicopter systems considering the input constraints and global prescribed ***, radial basis function NN(RBFNN) is employed to estimate the unknown dynamics of the helicopter system. Second, a smooth nonaffine function is exploited to approximate and address nonlinear constraint functions. Subsequently, a new prescribed function is proposed, and an original constrained error is transformed into an equivalent unconstrained error using the error transformation and barrier function transformation methods. The analysis of the established Lyapunov function proves that the controlled system is globally uniformly bounded. Finally, the simulation and experimental results on a constructed Quanser's test platform verify the rationality and feasibility of the proposed control.

关键词： adaptive NN control 2-DOF helicopter global prescribed performance input constraints

Hexamerin and allergen are required for female reproduction in the American cockroach,Periplaneta americana

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Insect Science 2024年第1期31卷 186-200页

作者： Shiming Zhu Xiaoyi Chen Sishi Xia Qin Li Ziqi Ye Shaoting zhao Kexin liu Fangfang liu Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologyInstitute of Insect Science and Technology&School of Life SciencesSouth China Normal UniversityGuangzhouChina Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhouGuangdong ProvinceChina Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhouChina

Reproduction is of great importance for the continuation of the *** insects,the fat body is the major tissue for nutrient storage and involved in vitellogenesis,which is essential for female ***,2 proteins,hexamerin and allergen,were separated from the fat bodies of adult female American cockroaches(Periplaneta americana)and identified as storage proteins,encoding for 733 amino acids with molecular weight of 87.88 kDa and 686 amino acids with molecular weight of 82.18 kDa,*** encoding genes of these 2 storage proteins are mainly expressed in the fat *** interference-mediated knockdown of Hexamerin and Allergen in the early stage of the first reproductive cycle in females suppressed vitellogenesis and ovarian maturation,indicating that these storage proteins are involved in controlling ***,the expression of Hexamerin and Allergen was repressed by knockdown of the juvenile hormone(JH)receptor gene Met and the primary response gene Kr-h1,and was induced by methoprene,a JH analog,in both in vivo and in vitro ***,we have determined that hexamerin and allergen are identified as storage proteins and play an important role in promoting female reproduction in the American *** expression of their encoding genes is induced by JH *** data reveal a novel mechanism by which hexamerin and allergen are necessary for JH-stimulated female reproduction.

关键词： fat body juvenile hormone ovary reproduction storage protein vitellogenesis