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Highly efficient transformation of intact yeast-like conidium cells of Tremella fuciformis by electroporation

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Science china(Life Sciences) 2008年第10期51卷 932-940页

作者： GUO LiQiong1.2, liu Yong1. {1. ShuXian1. liu ErXian1.& LIN JunFang1.2 1.college of Food Sciences, south china agricultural university, guangzhou 51.640, china 2 Institute of Biomass Research, south china agricultural university, guangzhou 51.640, china 1. college of Food Sciences South China Agricultural University Guangzhou 510640 China 2. Institute of Biomass Research South China Agricultural University Guangzhou 510640 China

Tremella fuciformis is one of higher basidiomycetes. Its basidiospore can reproduce yeast-like conidia, also called the blastospore by budding. The yeast-like conidia of T. fuciformis is monokaryotic and easy to culture by submerged fermentation similar to yeast. So it is a good recipient cell for exogenous gene expression. In this study, two expression vectors pGlg-gfp containing gpd-Gl promoter and gfp gene and pGlg-hph containing gpd-Gl promoter and hph gene were constructed. The lowest sensitive concentration of hygromycin for the blastospore was determined on three types of media. Our ex- periments showed that the lowest sensitive concentration of hygromycin for the blastospore was 5 μg/mL on MA medium. The intact blastospores were transformed with the expression vector pGlg-hph by electroporation. The putative transformants were obtained by the MA selective medium. Experi- mental results showed that the most effective parameters for the electroporation of intact blastospores were obtained by using STM buffer, 1.0×1.8 cells/mL of blastospores, 200 μL in transformation volume, 6 μg plasmid, 2.0 kV/cm of electric pulse voltage, stillness culturing on MB liquid medium for 48 h after electroporation. In these transformation conditions, the efficiency reached 277 colonies/μg DNA. Co-transformation of plasmid pGlg-gfp and pGlg-hph with ratio of 1.1.was performed by electroporation with the optimal parameters. The putative co-transformants were obtained by the MA selective medium. Eight randomly selected colonies from the vast putative co-transformants were analyzed by PCR de- tection and southern blotting. The experiments showed that the gfp was integrated into the genomes of three transformants. The co-transformation efficiency was 37.5%. Green fluorescence was observed under laser scanning confocal microscope in these gfp positive transformants. This indicates that the exogenous gfp can be expressed effectively in the yeast-like conidia of T. fuciformis.

关键词： Tremella fuciformis electroporation yeast-like conidia intact cells gene expression hph gene gfp gene

Molecular characterization and expression analysis of caveolin-1.in pig tissues

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Science china(Life Sciences) 2008年第7期51卷 655-661页

作者： WANG Chong1.MEI YingJie 1.LI Li 1.MO DeLin 2,LI JiaQi 1.ZHANG Hao 1.TIAN XingGuo 1.&CHEN YaoSheng2 1.0} of Animal Science,{1. china agricultural university,guangzhou 510642,china 2School of Life Science,Sun Yat-Sen university,guangzhou 51.006,china 1. college of Animal Science South China Agricultural University Guangzhou 510642 China 2. School of Life Science Sun Yat-Sen University Guangzhou 510006 China

Members of the caveolin family played important roles during fundamental cellular processes,such as regulation of cell morphology,migration,and gene expression in muscle *** this study,caveolin-1.(Cav-1.,one of the caveolins,was identified from longissimus dorsi muscle of Large Yorkshire pig and Chinese indigenous Lantang pig based on the results of mRNA differential display *** deduced amino acids sequence of the porcine Cav-1.contained a caveolin domain,and was very conservative among different *** Cav-1.mRNA was widely expressed in the eight tissues in this study,including heart,liver,kidney,encephalon,spleen,lung,longissimus dorsi muscle,and back fat, and the highest expression quantity was found in back fat of the two pig *** expression quantity of porcine Cav-1.in back fat and longissimus dorsi muscle of Lantang pig was significantly higher than that of Large Yorkshire(P1.and P1.might be a candidate gene for carcass traits,and might provide valuable information for understanding the mechanism of caveolae signaling in fat deposition by using the animal model of pig.

关键词： caveolin family clone expression analysis real-time quantify PCR

Early Cretaceous Metasomatized Lithospheric Mantle beneath the Central Jiangnan Orogen in south{1.:Geochemical and Sr-Nd Isotope Evidence from the Tuanshanbei Dolerite

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Acta Geologica Sinica(English Edition) 2024年第3期98卷 602-616页

作者： WANG Cheng WEI Hantao liu Qingquan YANG {1. Guangdong Provincial Key Laboratory of Geodynamics and Geohazards School of Earth Sciences and EngineeringSun Yat-sen UniversityGuangzhou 510275China Key Laboratory of Metallogenic Prediction of Nonferrous Metals and Geological Environment Monitoring Ministry of EducationSchool of Geosciences and Info-PhysicsCentral South UniversityChangsha 410083China Development and Research Centre China Geological SurveyBeijing 100037China School of Environmental Studies China University of GeosciencesWuhan 430074China

It is well established that Cretaceous magmatism in the south{1. Block(SCB)is related to the Paleo-Pacific ***,the starting time and the associated deep crust-mantle processes are still *** dike swarms carry important information on the deep earth(including mantle)geodynamics and geochemical *** the Jiangnan Orogen(south{1.).there is no information on whether the Mesozoic magmatic activities in this region are also directly related to the Pacific subduction or *** this study,we present detailed zircon U-Pb geochronological,wholerock element and Sr-Nd isotope data for Early Cretaceous Tuanshanbei dolerite dikes,and provide new constraints on the condition of the lithospheric mantle and mantle dynamics of the SCB during that ***-ICP-MS zircon U-Pb dating suggests that this dolerite erupted in the Early Cretaceous(~1.5 Ma).All samples have alkaline geochemical affinities with K_(2)O+Na_(2)O=3.1.-4.04 wt%,K_(2)O/Na_(2)O=0.50-0.72,and Mg^(#)=*** are enriched in LILE but depleted in HFSE with higher initial^(87)Sr/^(86)Sr ratio(0.706896-0.71.743)and lower ε_(Nd)(t)(-2.61.to-1.67).They have high Nb/U,Nb/La,La/Sm and Rb/Sr,and low La/Nb,La/Ta,Ce/Pb,Ba/Rb,Tb/Yb and Gd/Yb *** geochemical signatures suggest that the fractional crystallization is obvious but crustal contamination play a negligible role during magmatic *** dolerite were most likely derived from low-degree(2%-5%)partial melting of a phlogopite-bearing mantle material consisted of~85% spinel peridotite and~1.% garnet peridotite previously metasomatized by asthenospherederived fluids/melts with minor subduction-derived fluids/***-rollback generally lead to the upwelling of the hot *** upwelling of asthenosphere consuming the lithospheric mantle by thermo-mechanical-chemical *** lithospheric mantle may have partially melted due to the heating by the upwelling asthenosphere and lithospheric extension.I

关键词： Early Cretaceous mafic dike metasomatized lithospheric mantle Tuanshanbei dolerite Jiangnan Orogen south{1.

Chemical and physical properties of feather fiber

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Chemical and physical properties of feather fiber

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作者： Yang Chongling1. Guan Litao2, zhao Yaoming1.(1. {1. of Materials Science and Eng., south china university of Technology, guangzhou 51.640, china 2. {1. of Forestry, south china agricultural university, guangzhou 510642, china)

The chemical and physical properties of the feather fiber were evaluated with weight loss, breaking strength and breaking elongation. The results revealed, feather fiber has good resistance to acid. The concentration of 1.0mL/L H2SO4 solution almost had no effect on the physical properties of feat her fiber, while its resistance to alkaline was poor. Feather fiber was sensitive to oxidant. 0.5mL/L NaClO decreased the breaking strength and breaking elongation of feather fiber distinctly. However, 200mL/L NaHSO3 did not induce distinct changes to the physical properties of the feather fiber. This paper could be used as the referenced material and theoretical foundation for the processing of feather fibers, or for the development and standardization of the yarn of the feather fibers and the relative products.

关键词： feather fiber breaking strength breaking elongation

THE EVALUATION OF LEVELING CONTROL OF PADDY FIELD LEVELER BASED ON ULTRASONIC SENSORS

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THE EVALUATION OF LEVELING CONTROL OF PADDY FIELD LEVELER BA...

亚洲精细农业与计算机农业应用联合大会

作者： Hu Lian 1 2 Luo Xiwen 1 2 zhao Zuoxi 1 2 Li Qing 1 2 Chen Weitong 1 2 (1 Key Laboratory of Key Technology on agricultural Machine and Equipment Ministry of Education. south china agricultural university guangzhou 510642 china 2. college of Engineering south china agricultural university guangzhou 510642 china

Rice growing demands quite level fields due to its special growth requirements. When a tractor travels in a paddy field, the unevenness of the ground will cause roll angles. A paddy laser leveler was developed by south china agricultural university and the leveler features a specially leveling harrow which could turn from side to side by a cylinder. In order to estimate the leveling control system of paddy field leveler, the inclination of the harrow was measured using two SensComp 600 ultrasonic sensors. The ultrasonic sensors were fitted on the harrow’s two ends, respectively, each measuring the distance from ground, which acted as reference plane, to calculate the inclination angle. It was found that using two ultrasonic sensors would provide accurate inclination {1. of the harrow even when at non-right angles to the floor, and sensor in movement. The angles measured was compared with data from an attitude-heading reference system AHRS500GA-226 device used as a reference, the results showing that the calculated inclination was quite accurate: the maximum static-error and maximum dynamic-error were 0.08°and 1.respectively. Finally, estimation experiments were done in laboratory. Based on the evaluation results, it was concluded that the leveling control system measured inclination in real-time and precisely, the maximum error less than 1., and the whole leveling system works reliably.

关键词： Paddy Field Leveler Ultrasonic Sensor Distance Measurement Inclination Leveling Control

WOOD BOARD AUTO CLASSING SYSTEM BASED ON VIRTUAL INSTRUMENTATION TECHNOLOGY

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WOOD BOARD AUTO CLASSING SYSTEM BASED ON VIRTUAL INSTRUMENTA...

亚洲精细农业与计算机农业应用联合大会

作者： Weibin Wu 1.2 Tiansheng Hong 1.Shunzhi Guan 1.1.Key Laboratory of Key Technology on agricultural Machine and Equipment ({1. china agricultural university) Ministry of Education guangzhou 510642 china college of Engineering {1. china agricultural university guangzhou 510642 china 2 college of Mechanical and Automotive Engineering {1. china university of Technology 51.641.china

A wood board auto-classifying system basing on virtual instrumentation technology has been established. The system hardware is composed of optical probe, computer terminal, capture card, signal/adjustment models and instructions-executing unit. The software adopts the notion of modularization, and is altogether divided into four modules including master operational controls, automatic detections, information processing and miscellaneous functions, displaying page by page. Each is further subdivided into sub-modules, such as data storage, statement display and printing, history review, important parameter amendment （code needed for parameter value）, statistical analysis （maximum, minimum and average, etc.）, help system, user administration and so on. Its operating interface is a design by LabVIEW, the virtual instrumentation software with direct test results displayed on screen. The system applies a sensor with three receivers to detect color of object （wood board）, then output voltage. After conditioning, data acquisition card signal is collected to the PC. By the voltage and the calibration of the relationship between voltage and color stimulus value, computer terminal will calculate the stimulus value, and compared with the set value, then get the chromatic aberration. Finally, according to the size and scope of chromatic aberration divide it into 8 categories, output the corresponding switch control signal to the executing agency. This system can automatically measure wood board chromatism and output order to drive the enforcing unit accordingly, avoiding disadvantage as low accuracy and uneven color because of fatigue, dizziness or subjectiveness, in order to save cost and increase more sorting levels. Applications feature humanization, convenience and high-speed response to the system. The system and high-precision millivoltmeter （0.01.V） measure weak voltage at the same time, choosing forty-five points within 0 to 500mV for comparison, the absolute value of sy

关键词： Virtual Instrumentation Technology Data Acquisition Card Signal Conditioning Chromatism Wood Board Auto Classing

Soil Quality Assessment Using Weighted Fuzzy Association Rules

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Pedosphere 2010年第3期20卷 334-341页

作者： XUE Yue-Ju liu Shu-Guang HU Yue-Ming YANG Jing-Feng south{1. agricultural university Guangzhou 510642 (China) U.S. Geological Survey Center for Earth Resources Observation and Science Sioux Falls SD 57198 (USA) Geographic information Science Center of Excellence South Dakota State University Brookings SD 57007 (USA)

Fuzzy association rules (FARs) can be powerful in assessing regional soil quality, a critical step prior to land planning and utilization; however, traditional FARs mined from soil quality database, ignoring the importance variability of the rules, can be redundant and far from optimal. In this study, we developed a method applying different weights to traditional FARs to improve accuracy of soil quality assessment. After the FARs for soil quality assessment were mined, redundant rules were eliminated according to whether the rules were significant or not in reducing the complexity of the soil quality assessment models and in improving the comprehensibility of FARs. The global weights, each representing the importance of a FAR in soil quality assessment, were then introduced and refined using a gradient descent optimization method. This method was applied to the assessment of soil resources conditions in Guangdong Province, china. The new approach had an accuracy of 87%, when 1. rules were mined, as compared with 76% from the traditional approach. The accuracy increased to 96% when 32 rules were mined, in contrast to 88% from the traditional approach. These results demonstrated an improved comprehensibility of FARs and a high accuracy of the proposed method.

关键词： accuracy comprehensibility global weights

Transmit Power Minimization for IRS-Assisted NOMA-UAV Networks

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china Communications 2024年第5期21卷 137-152页

作者： zhao Chen Pang Xiaowei Tang Jie {1. Mingqian zhao Nan Zhang Xiuyin Wang Xianbin School of information and Communication Engineering Dalian University of TechnologyDalian 116024China School of Electronic and information Engineering South China University of TechnologyGuangzhou 510641China State Key Laboratory of Integrated Services Networks Xidian UniversityXi’an 710071China Department of Electrical and Computer Engineering Western UniversityLondonON N6A 5B9Canada

The flexibility of unmanned aerial vehicles(UAVs)allows them to be quickly deployed to support ground *** reflecting surface(IRS)can reflect the incident signal and form passive beamforming to enhance the signal in the specific *** by the promising benefits of both technologies,we consider a new scenario in this paper where a UAV uses non-orthogonal multiple access to serve multiple users with *** to their distance to the UAV,the users are divided into the close users and remote *** UAV hovers above the close users due to their higher rate requirement,while the IRS is deployed near the remote users to enhance their received *** aim at minimizing the transmit power of UAV by jointly optimizing the beamforming of UAV and the phase shift of IRS while ensuring the decoding ***,the problem is ***,we decompose it into two sub-problems,including the transmit beamforming optimization and phase shift optimization,which are transformed into second-order cone programming and semidefinite programming,*** propose an iterative algorithm to solve the two sub-problems *** results prove the effectiveness of the proposed scheme in minimizing the transmit power of UAV.

关键词： intelligent reflecting surface nonorthogonal multiple access phase shift optimization transmit beamforming optimization unmanned aerial vehicle

EXPERIMENTAL SYSTEM OF VEHICLE CAN BUS BASED ON J1.39 PROTOCOL

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EXPERIMENTAL SYSTEM OF VEHICLE CAN BUS BASED ON J1939 PROTOC...

亚洲精细农业与计算机农业应用联合大会

作者： Weibin Wu 1.2 Tiansheng Hong 1.Zili Zhang 1.Li Meng 1.1.Key Laboratory of Key Technology on agricultural Machine and Equipment ({1. china agricultural university) Ministry of Education guangzhou 510642 china college of Engineering {1. china agricultural university guangzhou 510642 china 2 college of Mechanical and Automotive Engineering {1. china university of Technology 51.641.china

Based on the training articles required by the J1.39 protocol, the training experimental system based on J1.39 vehicle bus protocol has been established. The part of hardware of the system is consist of the computer terminal, Kvaser CAN bus analyzer and a vehicle CAN bus module powered by J1.39 protocol, including the dashboard modules, vehicle-body electrical modules and the engine ECU modules. The Interface of the system is designed by X-Analyser powered by Warwick, and the results of the test can be displayed on the monitor directly. In allusion to the requirement of the training of J1.39, the system, especially during actual operation process, can do the real-time supervision on the vehicle J1.39 data and keep the entire record, display the waveforms and print them out. As to the change of display on the dashboard, the result is as followed: （1. the measurement and display of moving velocity and distance: system makes a real-time measurement of velocity and display the current velocity on the Velocity Dashboard, with unit of Km per hour. The impulse input of the moving- velocity sensor will reach to 200Hz while the moving-velocity elevates to the maximum value of 1.0Km per hour. When the velocity is not 0, system will make a real-time measurement of the number of distance of vehicles, and data will be displayed on the multi-functional LCD zone on the dashboard, with the unit of Km and accuracy of 0.1.m. Simultaneously, system keeps data recording for the total mileage the vehicle had run with interval of 1.m and the data will be displayed on the multi-functional LCD zone on the dashboard. （2） The measurement and display of the quantity of fuel: displaying data in the dimensionless way, the quantity of fuel is scaled by oil-mass sensor, marked by F which stands for Full and E which stands for Empty. When the resistance varies from 0 to 200Ω, the corresponding scale varies from F to E. （3） The measurement and display of the front and rear axle air pressure: the sy

关键词： J1.39 Protocol Vehicle CAN Bus Experimental System