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Bupleurum polysaccharides ameliorated renal injury in diabetic mice associated with suppression of HMGB1-TLR4 signaling

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Chinese Journal of Natural Medicines 2019年第9期17卷 641-649页

作者： liu zhen-zhen WENG Hong-Bo ZHANG Li-Jie PAN Ling-Yu SUN Wei CHEN Hai-Xia CHEN Mei-Yu ZENG Tao ZHANG Yun-Yi CHEN Dao-Feng LI Hong Department of Pharmacology School of PharmacyFudan UniversityShanghai 201203China Department of Pharmacognosy School of PharmacyFudan UniversityShanghai 201203China Clinical trial institution Obstetrics and Gynecology Hospital of Fudan UniversityShanghai 201203China

Bupleurum polysaccharides(BPs)is isolated from Bupleurum smithii ***,a key traditional Chinese *** study was to investigate the effects of BPs on diabetic kidney *** two intraperitoneal injections of streptozotozin(STZ)100 mg·kg^–1,renal injury in diabetic mice was induced and BPs was orally administrated at dosages of 30 and 60 mg·kg^–1·d^–*** STZ injected mice developed renal function damage,renal inflammation and fibrosis known as diabetic kidney disease(DKD).BPs significantly reduced serum creatinine level and urinary albumin excretion rate,with the attenuated swelling of *** treatment obviously alleviated the pathological damage of renal *** progression of renal injury in BPs treated mice was inhibited with less expression of type IV collagen(Col IV),fibronectin(FN)andα-smooth muscle actin(α-SMA).The inhibition of inflammation in kidney was associated with the reduced level of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6).BPs administration suppressed the over-expression of toll like receptor 4(TLR4)and high-mobility group box 1(HMGB1)with lowered activity of nuclear factor kappa B(NF-κB)in renal tissue of diabetic *** administration of BPs effectively prevented the development of renal injury in diabetic *** study suggested that the protection provided by BPs might affect through the interruption of HMGB1-TLR4 pathway,leading to the inhibition of renal inflammation and fibrotic process.

关键词： Renal injury Bupleurum:High-mobility group box Toll-like receptor 4 Inflammation Renal fibrosis Polysaccharides

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Enrichment of breast cancer stem cells using a keratinocyte serum-free medium

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Chinese Medical Journal 2011年第18期124卷 2934-2936页

作者： liu zhen-zhen CHEN Ping LU zhen-duo CUI Shu-de DONG Zi-ming Department of Pathophysiology Basic Medical CollegeZhengzhou University Zhengzhou Henan 450053 China Breast Cancer Tenter Henan Cancer Hospital and Affiliated Cancer Hospital of Zhengzhou University Zhengzhou Henan 450008 China

Background Keratinoyte serum-free medium （K-SFM） is a defined medium used to support the growth of primary keratinocytes and embryonic stem cell. The aim of this research was to optimize enrichment of breast cancer stem cells （CSCs） using K-SFM. Methods＇ A K-SFM was used to enrich CSCs from two breast cancer cell lines and a primary culture of breast cancer. RPMI-1640 supplemented with 10% fetal calf serum （FCS） was used as a control. CSCs were identified with flow cytometry using CD44＋/CD24-as molecular markers. The expression of a variety of CSC markers （Oct-4, ABCG2, Nanog, N-cadherin, and E-cadherin） was analyzed with real-time PCR. Results Much higher percentage of CSCs was achieved with K-SFM： 17.3% for MCF-7 cells, 17.4% for SKBR-3, and 20.0% for primary breast cancer culture. Less than 1% CSC was achieved using RPMI-1640 supplemented with 10% FCS. In comparison to the CSCs obtained with RPMI-1640, CSCs in the K-SFM expressed higher levels of Oct-4, ABCG2, Nanog and N-cadherin, and lower level of E-cadherin. Conclusion K-SFM is an optimal culture medium to maintain and to enrich breast CSCs.

关键词： breast cancer cancer stem cells keratinocyte serum-free medium defined medium, enrichment

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稀土粗精矿的低温硫酸化分解工艺

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中国有色金属学报 2014年第12期24卷 3147-3154页

作者：刘勇刘珍珍刘牡丹广州有色金属研究院资源综合利用研究所广州510650 广州有色金属研究院稀有金属分离与综合利用国家重点实验室广州510650

对物理选矿和化学选矿得到的两种稀土粗精矿进行低温酸化工艺研究,通过正交和单因素实验研究酸矿比(浓硫酸与稀土精矿的质量比)、酸化时间、酸化温度以及液固比对稀土浸出效果的影响。结果表明,酸矿比、酸化时间和酸化温度对稀土粗精矿... 详细信息

对物理选矿和化学选矿得到的两种稀土粗精矿进行低温酸化工艺研究,通过正交和单因素实验研究酸矿比(浓硫酸与稀土精矿的质量比)、酸化时间、酸化温度以及液固比对稀土浸出效果的影响。结果表明,酸矿比、酸化时间和酸化温度对稀土粗精矿稀土浸出率的影响显著,最佳条件如下:酸矿质量比1.8:1(精矿Ⅰ)和0.9:1(精矿Ⅱ),酸化时间2.0 h(精矿Ⅰ)和3.0 h(精矿Ⅱ),酸化温度150℃。在优化的酸化和浸出条件下,稀土的浸出率分别为90.42%(精矿Ⅰ)和91.35%(精矿Ⅱ)。为研究影响稀土溶出的因素,采用MLA工艺矿物学自动检测系统对优化条件下两种粗精矿酸化浸出后的残渣进行矿物组分的定性分析,证明稀土与硫酸钙形成了复杂难溶硫酸复盐,这是影响稀土浸出率的关键因素。

关键词：稀土粗精矿浸出酸化浸出率

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Human U Three Protein 14a Expression is Increased in Hepatocellular Carcinoma and Associated with Poor Prognosis

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Chinese Medical Journal 2017年第4期130卷 470-476页

作者： Jing-Yi Zhang Da Xu zhen-zhen liu Yuan Li Li-Jun Wang Bao-Cai Xing Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Hepatopancreatobiliary Surgery Department I Peking University Cancer Hospital and Institute Beijing 100142 China

Background： Human U three protein 14a （hUTP 14a） promotes p53 degradation. Moreover, hUTP 14a expression is upregulated in several types of tumors. However, the expression pattern of hUTP 14a in hepatocellular carcinoma （HCC） remains unknown. The aim of this study was to investigate hUTP 14a expression and its prognostic value in HCC. Methods： The hUTP 14a expression was evaluated using immunohistochemistry （IHC） in HCC tissue specimens. The correlations between hUTP 14a expression and clinicopathological variables were analyzed. The Kaplan-Meier method was used to analyze the association between hUTP14a expression and survival. Independent prognostic factors associated with overall survival （OS） and disease-free survival （DFS） were analyzed using the Cox proportional-hazards regression model. Results： The IHC data revealed that the hUTP 14a positivity rate in HCC tissue specimens was significantly higher than that in nontumorous tissue specimens （89.9% vs. 72.7%, P 〈 0,05）. The hUTP14a expression was detected in both the nucleolus and the cytoplasm. The positivity rate of nucleolar hUTP14a expression in HCC tissue specimens was higher than that in the nontumorous tissue specimens （29.3% vs. 10.1%, P 〈 0.05）. No significant difference was found between HCC and nontumorous tissue specimens of cytoplasmic hUTP 14a expression （60.6% vs. 62.6%, P 〉 0.05）. In addition, no significant correlation was found between nucleolar hUTP 14a expression and other clinicopathological variables. The 5-year OS and DFS rates in patients with positive nucleolar hUTP14a expression were significantly lower than those in patients with negative hUTP 14a expression （P = 0.004 for OS, P = 0.003 for DFS）. Multivariate analysis showed that nucleolar hUTP 14a expression was an independent prognostic factor for OS （P = 0.004） and DFS （P 〈 0.001 ）. Conclusions： The positivity rate of hUTP 14a expression was significantly higher in HCC specimens. Positive expression of nucleolar hUTP 14a might act

关键词： Hepatocellular Carcinoma Human U Three Protein 14a Poor Prognosis

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High risk factors of brain metastases in 295 patients with advanced breast cancer

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Chinese Medical Journal 2013年第7期126卷 1269-1275页

作者： YAN Min Lo Hui-min liu zhen-zhen liu Hui ZHANG Meng-wei SUN Xi-bin CUI Shu-de Henan Breast Cancer Center Affiliated Cancer Hospital of Zhengzhou University Zhengzhou Henan 450008 China Henan Cancer Hospital Affiliated Cancer Hospital of Zhengzhou University Zhengzhou Henan 450008 China

Background The incidence of brain metastases in patients with breast cancer is approximately 10%-16%, and survival after diagnosis of brain metastases is usually short. This study was designed to evaluate the risk factors associated with brain metastases in advanced breast cancer patients, with a view to help predict patient groups with high risk of brain metastases. Methods In total, 295 patients with advanced breast cancer were evaluated. All patients were pathologically confirmed and metastatic lesions were confirmed pathologically or by imaging. All patients were examined at least once every 6 months with head CT or MRI. Patients showing symptoms underwent immediate inspection, and brain metastatic lesions were confirmed by head CT and/or MRI. Results At a median follow-up of 12 months from the occurrence of metastases, brain metastases had occurred in 49 patients （16.6%）. In our univariate analysis, variables significantly related to increased risk of brain metastases were hormone receptor-negative tumors, epidermal growth factor receptor 2 （HER2）-positive tumors, and multiple distant metastases. Patients with dominant tumor sites in soft tissue, or defined as Luminal A subtype, tended to have a lower risk of brain metastases than patients with visceral metastases, Luminal B subtype, triple-negative subtype or HER2-enriched subtype tumors. Conclusions Our results strongly suggest that factors such as Luminal B, triple-negative, and HER2-enriched subtypes are high risk factors for brain metastases. These data, therefore, provide pivotal clinical evidence towards a comprehensive understanding of the risk factors of brain metastases in advanced breast cancer patients.

关键词： brain metastases breast cancer Luminal A Luminal B triple-negative HER2 enriched

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全回转拖轮舱室振动噪声预报与控制

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船舶力学 2018年第10期22卷 1292-1299页

作者：温华兵刘甄真陈宁江苏科技大学能源与动力工程学院江苏镇江212003

分析了某全回转拖轮的主要振动噪声源,采用有限元法建立了船体结构的超单元模型,计算了船体结构的振动响应。采用统计能量法建立了舱室噪声预报模型,计算了各舱室的噪声分布,通过相同船型的实验测量验证了仿真模型。舱室振动噪声预报结... 详细信息

分析了某全回转拖轮的主要振动噪声源,采用有限元法建立了船体结构的超单元模型,计算了船体结构的振动响应。采用统计能量法建立了舱室噪声预报模型,计算了各舱室的噪声分布,通过相同船型的实验测量验证了仿真模型。舱室振动噪声预报结果表明,设计的主机隔振方案可达到4～5 dB的减振和4～10 dB的降噪效果,设计的舱室吸声降噪方案可达到5～7 dB的降噪效果,主机隔振和舱室吸声降噪综合方案可达到近10 dB的降噪效果,显著改善了舱室振动噪声水平。

关键词：全回转拖轮有限元法统计能量法振动响应舱室噪声预报隔振

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译者的诗学态度与跨文化翻译中诗学的赋值

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湖南科技大学学报（社会科学版） 2020年第5期23卷 104-110页

作者：刘珍珍南京大学外国语学院江苏南京210023 淮阴师范学院外国语学院江苏淮安223399

译入语诗学规范、原语诗学文化地位以及译者的诗学态度会影响文学译者的翻译实践。余国藩对《西游记》中诗词的翻译,其在韵律、句构以及修辞层面的转换策略极具诗学赋值。对诗学态度与韵文翻译策略的关联进行解读,可以揭示译者在诗学重... 详细信息

译入语诗学规范、原语诗学文化地位以及译者的诗学态度会影响文学译者的翻译实践。余国藩对《西游记》中诗词的翻译,其在韵律、句构以及修辞层面的转换策略极具诗学赋值。对诗学态度与韵文翻译策略的关联进行解读,可以揭示译者在诗学重构过程中主观能动性的存在及重要作用,为中国文学译介中的诗学问题处理提供一定的借鉴和启示。

关键词：《西游记》诗学态度韵文翻译策略

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A Brief Talk about Some of Translation Skills Used in the Two Chinese Versions of Rip Van Winkle

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海外英语 2017年第7期 150-152页

作者：刘贞贞河南大学

Rip Van Winkle is one of the short novels written in the earliest American *** author Washington Irving(1783-1859)is celebrated as the father of American *** novel has strong local flavor and romantic *** paper will analyze thetranslation technique of Linshu and Wanzi,Yuning's *** will take some examples to analyze the translation techniques of twoversions based on the translations of Linshu and Wan Zi,Yu Ning.

关键词： Rip Van Winkle technique of translation version Linshu Wanzi Yuning

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狂犬病疫苗蔗糖密度梯度离心纯化工艺开发

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中国生物工程杂志 2020年第4期40卷 25-33页

作者：刘珍珍田大勇上海青赛生物科技有限公司上海201506

目的:开发冻干人用狂犬病疫苗(鸡胚成纤维细胞)的连续流蔗糖密度梯度离心纯化工艺。方法:分别比较两种不同初始蔗糖浓度和不同上样速度对纯化效果的影响,初步确定纯化工艺;通过多批次实验确定样品收集范围;比较不同浓缩倍数条件下杂质... 详细信息

目的:开发冻干人用狂犬病疫苗(鸡胚成纤维细胞)的连续流蔗糖密度梯度离心纯化工艺。方法:分别比较两种不同初始蔗糖浓度和不同上样速度对纯化效果的影响,初步确定纯化工艺;通过多批次实验确定样品收集范围;比较不同浓缩倍数条件下杂质去除和抗原回收情况,确定合适的收获液浓缩比例;比较不同批次样品纯化后的杂质去除率和重复性,判定本纯化工艺的稳定性。结果:选取60%作为初始蔗糖浓度,在上样速度为150~200ml/min时,可以有效地对10倍浓缩的病毒收获液进行纯化;卵清蛋白、牛血清白蛋白和庆大霉素去除率分别达到99%,95%和95%,且工艺具有极好的稳定性。结论:开发的连续流蔗糖密度梯度离心技术可以作为冻干人用狂犬病疫苗(鸡胚成纤维细胞)的产业化纯化工艺。

关键词：狂犬病病毒疫苗纯化工艺连续流蔗糖密度梯度离心

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Clinical practice guidelines for modified radical mastectomy of breast cancer: Chinese Society of Breast Surgery (CSBrs) practice guidelines 2021

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Chinese Medical Journal 2021年第8期134卷 895-897页

作者： De-Chuang Jiao Jiu-Jun Zhu Li Qin Xu-Hui Guo Ya-Jie Zhao Xiu-Chun Chen Cheng-zheng Wang zhen-Duo Lu Lian-Fang Li Shu-De Cui zhen-zhen liu Department of Breast Disease Henan Breast Cancer CenterAffiliated Cancer Hospital of Zhengzhou University&Henan Cancer HospitalZhengzhouHenan 450003China.

The proportion of patients with early-stage breast cancer undergoing mastectomy exceeds 70%in China/11 among which Auchincloss operation is the major surgical technique for patients with axillary lymph node positive breast *** order to standardize the clinical application of modified radical mastectomy of breast cancer,Chinese Society of Breast Surgery(CSBrS)organized domestic experts to conduct literature retrieval and expert discussion on the theoretical basis and technical details of modified radical mastectomy for breast *** reference to the grading of recommendations assessment,development,and evaluation system。

关键词： Surgery mastectomy breast

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