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An association study of HFEgene mutation with idiopathic male infertility in the Chinese Han population

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Asian Journal of Andrology 2012年第4期14卷 599-603页

作者： Xiao-ying yu Bin-Bin Wang Zhong-cheng Xin Tao liu Ke Ma Jian Jiang xiang Fang Li-hua yu Yi-Feng Peng Xu Ma Institute of Reproductive Medicine Yijishan HospitalWannan Medical CollegeWuhu 241001China National Research Institute for Family Planning Beijing 100081China peking Union Medical College Beijing 100081China Andrology Center Peking University First HospitalBeijing 100034China World Health Organization Collaborating Centre for Research in Human Reproduction Beijing 100081China

Mutations in the haemochromatosis gene （HFE） influence iron status in the general population of Northern Europe, and excess iron is associated with the impairment of spermatogenesis. The aim of this study is to investigate the association between three mutations （C282Y, H63D and S65C） in the HFEgene with idiopathic male infertility in the Chinese Han population. Two groups of Chinese men were recruited： 444 infertile men （including 169 with idiopathic azoospermia） and 423 controls with proven fertility. The HFEgene was detected using polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） technique. The experimental results demonstrated that no C282Y or $65C mutations were detected. Idiopathic male infertility was not significantly associated with heterozygous H63D mutation （odds ratio=O.801, 95% confidence interval=0.452-1.421, X2=0.577, P=0.448）. The H63D mutation frequency did not correlate significantly with the serum luteinizing hormone （LH）, follicle-stimulating hormone （FSH） and testosterone （T） levels in infertile men （P=0.896, P=0.404 and P=O.05, respectively）. Our data suggest that the HFEH63D mutation is not associated with idiopathic male reproductive dysfunction.

关键词： gene mutation gonadotropic hormone HFE male infertility

The Cloning and Fluorescence In situ HybridizationAnalysis of Cotton Telomere Sequence

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Journal of Integrative Agriculture 2012年第9期11卷 1417-1423页

作者： LING Jian cheng hua liu Fang SONG Guo-li WANG Chun-ying LI Shao-hui ZHANG xiang-di WANG yu-hong WANG Kun-bo State Key Laboratory of Cotton Biology~Cotton Research Institute Chinese Academy of Agricultural Sciences Anyang 455000 P.R.China Anyang Institute of Technology Anyang 455000 P.R.China

Telomeres form the ends of eukaryotic chromosomes and serve as protective caps that keep chromosomes structure independency and completeness. The first plant telomere DNA was isolated from Arabidopsis thaliana and was shown to have tandemly repeated sequence 5-TTTAGGG-3： The Arabidopsis-type telomere has been found in many plants, but several reports indicate that this sequence is absent in some plants. Up to now, no research has been conducted on the telomere of cotton. In this paper, the Arabidopsis-type telomere sequence was amplified and cloned using the primers designed based on the fragment containing telomere sequence in an Arabidopsis bacterial artificial chromosome （BAC）. Fluorescence in situ hybridization （FISH） with cotton metaphase chromosomes using the Arabidopsis-type telomere sequence as probes indicated that the signals were located at all chromosome ends of seven diploid and two tetraploid cotton species with different signal intensities among chromosome complements of different cotton species, even between long and short arms of the same chromosome. To identify the signals of FISH, the genome DNA of Xinhai 7, a cultivar of Gossypium barbadense, digested by BAL-31 nuclease was introduced in this study. The result of BAL-31 digestion indicated that the hybridization signals of FISH represent the outermost DNA sequence of each cotton chromosomes. So we first proved that the telomeric repeats of cotton cross-hybridize with that of Arabidopsis. The results of terminal restriction fragment （TRF） showed significant variation in telomere length among cotton species. The telomere length of cultivated cotton was close to 20 kb and was larger than those of wild cotton species whose telomere length rahged from 6 to 20 kb.

关键词： cotton fluorescent in situ hybridization (FISH) telomere terminal restriction fragment (TRF)

Immunogenicity of Lyophilized MVA Vaccine for HIV-1 in Mice Model

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Chemical Research in Chinese Universities 2007年第3期23卷 329-332页

作者： ZHANG Yi-zhe JIANG Chun-lai yu xiang-hui LOU Chao-ping ZHAO Dong-hai WU Yong-ge JIN ying-hua liu cheng-shan KONG Wei Vaccine Research Center College of Life Science Jilin University Changchun 130021 P. R. China

Highly attenuated modified vaccinia Ankara（MVA） is sensitive to repeat freeze-thaw cycle and easy to lose activity. In order to make the activity of MVA vaccine remain stable during its manufacturing, storage, and administration, the lyophilization as a good option could be resorted to; through screening, the right stabilizer composition and its production procedure were obtained. The final moisture content of freezing-dried recombinant MVA-HIV vaccine was lower than 3%. It can be reconstituted quickly and shows regular physical appearance and stable potency. In vivo functional experiment, mice were divided randomly into the liquid vaccination group, the lyophilized vaccination group, and the control group. Having been DNA vaccine priming, the mice were boosted with a dose of 10^7 pfu MVA- HIV vaccine, which produced indistinguishable antibody titer and cytotoxic T-lymphocyte（CTL） level compared with those of liquid vaccination group （ P 〉 0.05 ）. These results demonstrate that lyophilized MVA vaccine can induce high immunogenicity in mice.

关键词： Recombinant MVA Vaccine Lyophilize Immunogenicity Stability

地尔硫卓对心脏缺血/再灌注后心肌炎症的影响

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中国药理学通报 2010年第1期26卷 56-59页

作者：刘英程翔廖玉华华中科技大学协和医院心内科湖北武汉430022

目的探讨地尔硫卓对心脏缺血/再灌注后炎症细胞和细胞因子表达的影响。方法建立大鼠缺血/再灌注模型,存活鼠随机分为地尔硫卓组(D组)和缺血/再灌注组(I/R组),另设假手术组(S组)。分别在术后1、2和4wk检测各组大鼠超声心动图、心肌炎症... 详细信息

目的探讨地尔硫卓对心脏缺血/再灌注后炎症细胞和细胞因子表达的影响。方法建立大鼠缺血/再灌注模型,存活鼠随机分为地尔硫卓组(D组)和缺血/再灌注组(I/R组),另设假手术组(S组)。分别在术后1、2和4wk检测各组大鼠超声心动图、心肌炎症细胞浸润及致炎因子IL-1β、TNF-α、IL-6及抗炎因子IL-10、TGF-β的表达。结果与IR组比较,超声心动图显示D组射血分数和左室重量明显改善,心肌组织炎症细胞浸润减少,致炎因子TNF-α、IL-1β、IL-6表达减少,抗炎因子IL-10、TGF-β呈现低水平表达。结论地尔硫卓减少心肌缺血;再灌注损伤后炎症细胞浸润和致炎因子表达,保护心脏功能。

关键词：地尔硫卓缺血/再灌注细胞因子炎症细胞大鼠

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钙通道阻滞剂与心血管免疫炎症的关系

中国药理学通报 2007年第2期23卷 141-143页

作者：刘英程翔廖玉华华中科技大学同济医学院心血管病研究所协和医院心内科湖北武汉430022

近年来发现钙离子活动与心血管免疫和炎症有密切关系,钙通道阻滞剂对心血管免疫炎症的影响正逐渐受到研究者的关注。该文分别阐述了心血管免疫炎症机制、钙离子在免疫炎症中的作用、钙通道阻滞剂对心血管免疫炎症的影响及其未来在心血... 详细信息

近年来发现钙离子活动与心血管免疫和炎症有密切关系,钙通道阻滞剂对心血管免疫炎症的影响正逐渐受到研究者的关注。该文分别阐述了心血管免疫炎症机制、钙离子在免疫炎症中的作用、钙通道阻滞剂对心血管免疫炎症的影响及其未来在心血管病应用中新的意义。

关键词：钙通道阻滞剂心血管病免疫炎症

地尔硫卓对刀豆凝集素诱导单个核细胞细胞因子表达的影响

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中国药理学通报 2010年第3期26卷 376-378页

作者：刘英程翔廖玉华华中科技大学协和医院心内科湖北武汉430022

目的探讨地尔硫卓对刀豆凝集素(ConA)诱导单个核细胞细胞因子表达的影响。方法ficoll密度梯度离心法分离大鼠脾脏单个核细胞,分对照组、ConA组和地尔硫卓-ConA处理组。ELISA测定上清中细胞因子浓度。结果单个核细胞在ConA的刺激下,其上... 详细信息

目的探讨地尔硫卓对刀豆凝集素(ConA)诱导单个核细胞细胞因子表达的影响。方法ficoll密度梯度离心法分离大鼠脾脏单个核细胞,分对照组、ConA组和地尔硫卓-ConA处理组。ELISA测定上清中细胞因子浓度。结果单个核细胞在ConA的刺激下,其上清液中IL-10、TNF-α、IL-6急剧增高,IL-1β低水平表达,TGF-β1未见明显表达。地尔硫卓干预后,IL-10、TNF-α、IL-6的浓度明显减低。结论地尔硫卓能抑制ConA所诱导的单个核细胞IL-10、TNF-α、IL-6的表达。

关键词：地尔硫卓细胞因子单个核细胞刀豆凝集素 T淋巴细胞钙

心肌钙反常模型细胞因子的表达及地尔硫卓的干预作用

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中国药理学通报 2010年第3期26卷 309-311页

作者：刘英程翔廖玉华华中科技大学协和医院心内科湖北武汉430022

目的探讨心肌钙反常模型诱导心肌细胞因子的表达及地尔硫卓的干预作用。方法采用Langendorff装置对离体大鼠心脏进行钙反常灌流(无钙灌流液5min,立即换正常钙灌流液灌流30min),建立心肌细胞钙超载模型。设立钙超载组、正常对照组、无钙... 详细信息

目的探讨心肌钙反常模型诱导心肌细胞因子的表达及地尔硫卓的干预作用。方法采用Langendorff装置对离体大鼠心脏进行钙反常灌流(无钙灌流液5min,立即换正常钙灌流液灌流30min),建立心肌细胞钙超载模型。设立钙超载组、正常对照组、无钙对照组、钙超载给药组(地尔硫卓)和无钙给药组。电镜和光镜观察心肌病理和超微结构的变化。原子分光光度计检测心肌钙离子浓度,RT-PCR法测心肌细胞因子TNF-α、IL-1β、IL-6、TGF-β1和IL-10的表达。结果钙超载组光镜下未见明显炎症细胞浸润,电镜结果显示心肌细胞膜、细胞核和线粒体明显破坏。与正常对照组比较,TNF-α、IL-1β和IL-6明显增高,而TGF-β1和IL-10无明显变化。钙超载给药组(地尔硫卓)显示心肌损伤明显改善,TNF-α、IL-1β和IL-6明显下降。正常对照组、无钙对照组和无钙给药组心肌光镜和电镜下无明显差异,心肌Ca2+浓度差异无统计学意义,细胞因子的表达差异也无统计学意义。结论心肌钙反常模型诱导心肌表达TNF-α、IL-1β和IL-6,对TGF-β1和IL-10无明显影响,地尔硫卓能抑制心肌细胞钙超载诱导的TNF-α、IL-1β和IL-6表达。

关键词：地尔硫卓钙超载炎症细胞肿瘤坏死因子-α 白细胞介素-1β 白细胞介素-6

Influence of Air Pollution on Hospital Admissions in Adult Asthma in Northeast China

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Chinese Medical Journal 2018年第9期131卷 1030-1033页

作者： ying liu Hao-Dong Wang Zhen-xiang yu Shu-cheng hua Li-Ting Zhou Li-Ping Peng Department of Respiratory Medicine The First Hospital of Jilin University Jilin University Changchun Jilin 130021 China Department of Vascular Surgery The First Hospital of Jilin University Jilin University Changchun Jilin 130021 China Department of Occupational and Environmental Health School of Public Health Jilin University Changchun Jilin 130021 China

Background：Asthma is a common chronic respiratory disease and is related to air pollution ***,only a few studies have concentrated on the association between air pollution and adult ***,the results of these studies are ***,the present study aimed to analyze the influence of various pollutants on hospitalization due to asthma in ***：A total of 1019 unrelated hospitalized adult asthma patients from Northeast China were recruited from 2014 to *** average concentrations of air pollutants （particulate matter ＜2.5 μm [PM2.5],particulate matter ＜ 10 μm [PM 10],sulfur dioxide [SO2],nitrogen dioxide [NO2],and carbon monoxide [CO]） were obtained from the China National Environmental Monitoring Centre website from 2014 to *** logistic regression analysis was used to analyze the relationship between air pollutants and hospital admissions in adult ***：The maximum odds ratio （OR） value for most air pollutants occurred on lag day *** day 1 was chosen as the exposure period,and 8 days before onset was chosen as the control *** pollutants （PM2.5,CO,and SO2） were entered into the regression equation,and the corresponding OR （95％ confidence interval） was 0.995 （0.991-0.999）,3.107 （1.607-6.010）,and 0.979 （0.968-0.990）,***：A positive association between hospital admissions and the daily average concentration of CO was *** is likely to be a risk factor for hospital admissions in adults with asthma.

关键词： Air Pollution Asthma Carbon Monoxide Particulate Matter Sulfur Dioxide

Application of deep learning methods combined with physical background in wide field of view imaging atmospheric Cherenkov telescopes

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Nuclear Science and Techniques 2024年第4期35卷 208-220页

作者： Ao-Yan cheng Hao Cai Shi Chen Tian-Lu Chen xiang Dong You-Liang Feng Qi Gao Quan-Bu Gou Yi-Qing Guo Hong-Bo Hu Ming-Ming Kang Hai-Jin Li Chen liu Mao-yuan liu Wei liu Fang-Sheng Min Chu-cheng Pan Bing-Qiang Qiao xiang-Li Qian Hui-ying Sun yu-Chang Sun Ao-Bo Wang Xu Wang Zhen Wang Guang-Guang Xin yu-hua Yao Qiang yuan Yi Zhang School of Physics and Technology Wuhan UniversityWuhan 430072China School of Physics and Astronomy Yunnan UniversityKunming 650091China Ministry of Education The Key Laboratory of Cosmic Rays(Tibet University)Lhasa 850000China Key Laboratory of Particle Astrophysics Institute of High Energy PhysicsChinese Academy of SciencesBeijing 100049China University of Chinese Academy of Sciences 19 A Yuquan RoadShijingshan DistrictBeijing 100049China College of Physics Sichuan UniversityChengdu 610064China School of Intelligent Engineering Shandong Management UniversityJinan 250357China Tsung‑Dao Lee Institute Shanghai Jiao Tong UniversityShanghai 200240China Suzhou Aerospace Information Research Institute Suzhou 215123China College of Physics Chongqing UniversityNo.55 Daxuecheng South RoadHigh‑tech DistrictChongqing 401331China Key Laboratory of Dark Matter and Space Astronomy Purple Mountain ObservatoryChinese Academy of SciencesNanjing 210008China

The High Altitude Detection of Astronomical Radiation(HADAR)experiment,which was constructed in Tibet,China,combines the wide-angle advantages of traditional EAS array detectors with the high-sensitivity advantages of focused Cherenkov *** objective is to observe transient sources such as gamma-ray bursts and the counterparts of gravitational *** study aims to utilize the latest AI technology to enhance the sensitivity of HADAR *** datasets and models with distinctive creativity were constructed by incorporating the relevant physical theories for various *** models can determine the type,energy,and direction of the incident particles after careful *** obtained a background identification accuracy of 98.6%,a relative energy reconstruction error of 10.0%,and an angular resolution of 0.22°in a test dataset at 10 *** findings demonstrate the significant potential for enhancing the precision and dependability of detector data analysis in astrophysical *** using deep learning techniques,the HADAR experiment’s observational sensitivity to the Crab Nebula has surpassed that of MAGIC and *** energies below 0.5 TeV and remains competitive with conventional narrow-field Cherenkov telescopes at higher *** addition,our experiment offers a new approach for dealing with strongly connected,scattered data.

关键词： VHE gamma-ray astronomy HADAR Deep learning Convolutional neural networks