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Functional characterization of pheromone receptor candidates in codling moth Cydia pomonella(Lepidoptera:Tortricidae)

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Insect Science 2021年第2期28卷 445-456页

作者： Ke Tian Wei liu Li-Kai Feng Tian-yu Huang {2.-Rong Wang Ke-Jian Lin State key laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijingChina institute of Plant Protection Xinjiang Academy of Agricultural and Reclamation ScienceShiheziXinjiang Uygur Autonomous RegionChina Lingnan Guangdong laboratory of Modern Agriculture Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong ProvinceChina

Sex pheromones serve a critical role in Lepidopterans finding *** moths perceive and react to sex pheromones emitted by conspecific females through a delicate pheromone communication *** receptors(PRs)are the key sensory elements at the beginning of that *** codling moth(Cydia ponwonella)is an important pome fruit pest globally and a serious invasive species in *** techniques have been used successfully in monitoring and controlling this *** conducted ribonucleic acid sequencing analysis of the codling moth antennal transcriptome and identified 66 odorant receptors(ORs)in a population from Xinjiang province,china,of which 14 were PRs,including two novel PRs(CpomOR2. and CpomOR73).Four PRs that contain full-length open reading frames(CpomORl,OR2.,OR5,OR7)and four PRs with ligands that have not been reported previously(CpomORl,OR2.,OR5,OR7)were selected to deorphanize in the heterologous Xenopus oocyte expression ***,we found that CpomOR2. and CpomOR5 responded to(£,£)-8,10-dodecadien-l-yl acetate(codlemone acetate).Furthermore,CpomOR5(EC50=1.379 x 10-8 mol/L)was much more sensitive to codlemone acetate than CpomOR2.(EC50=1.663×10^(-6) mol/L).Since codlemone acetate is an important component of *** sex pheromone,our results improve the current understanding of pheromone communication in codling moths and will be helpful for the development of pest management strategies.

关键词： antennal transcriptome codlemone acetate Cydia pomonella',pheromone receptor

Activation of Carbon Dioxide by Gas-phase Metal Species

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chinese Journal of Structural chemistry 2021年第10期40卷 1385-1403页

作者： liu yun-Zhu LI Xiao-Na HE Sheng-{2. State key laboratory for Structural Chemistty of Unstable and Stable Species Institute of ChemistryChinese Academy of SciencesBeijing 100190China University of chinese academy of sciences Beijing 100049China beijing national {2. for molecular sciences and CAS Research/Educatiou Center of Excellence in molecular sciences Beijing 100190China

Catalytic conversion of carbon dioxide(CO_(2.) into value-added chemicals is an important and active field in both of the condensed-phase and gas-phase studies. This mini-review summarizes a variety of experimentally identified reactions in the activation and transformation of CO_(2. by metal species in the gas phase. The use of advanced mass spectrometric instrumentation in conjunction with quantum chemistry calculations can uncover the mechanistic details and determine the vital factors that control the activation of CO_(2.. This review focuses mainly on three topics: the activation of CO_(2. by(1) bare metal ions and metal oxide species,(2. metal hydrides, and(3) other gas-phase metal species. Emphasis is placed on the latest advances in the hydrogenation of CO_(2. mediated with metal hydrides. A potential prospect toward the future effort in the activation and transformation of CO_(2. in gas phase has also been discussed.

关键词： carbon dioxide activation gas-phase metal species mass spectrometry quantum chemistry calculations

lncRNA-PACER upregulates COX-2.and PGE2.through the NF-κB pathway to promote the proliferation and invasion of colorectal-cancer cells

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Gastroenterology Report 2021年第3期9卷 257-268,I0003页

作者： Peng Sun Ji-Chuan Quan Song Wang Meng Zhuang Zheng liu Xu Guan gui-{2. Wang Hong-ying Wang Xi-Shan Wang Department of Colorectal Surgery The Second Affiliated Hospital of Harbin Medical UniversityHarbinHeilongjiangP.R.China Department of Gastrointestinal Surgery Shenzhen HospitalNational Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeShenzhenGuangdongP.R.China Department of Colorectal Surgery National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Department of State key laboratory of {2. Oncology National Cancer Center/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China

Background p50-associated cyclooxygenase-2.extragenic RNA(PACER)is a recently identified antisense long non-coding RNA(lncRNA)located on the upstream of the promoter region of cyclooxygenase-2.COX-2..Preliminary studies have suggested that PACER is involved in the regulation of COX-2.expression in macrophagocyte and osteosarcoma ***,the role of this lncRNA in colorectal cancer(CRC)remains ***,we investigated the expression of PACER and its effect on cell proliferation and invasion to explore the role of PACER in *** Real-time quantitative PCR(RT-qPCR)analysis was used to evaluate the expression of PACER in CRC tissues and *** thiazolyl tetrazolium(MTT)analysis was then used to investigate the inhibition effect of PACER knock-down in cell *** promoting role of this lncRNA on invasion by CRC cells was analysed by wound-healing assays,colony-formation assay,and transwell *** then used fluorescence in situ hybridization(FISH)to establish the subcellular localization of ***-2.protein levels were quantified by Western blot analysis and grayscale scanning analysis following the knock-down of *** assay was carried out to monitor the modulation of the COX-2.promoter region by *** xenografts models were used to investigate the impact of PACER on the tumorigenesis of CRC cells in ***-linked immunosorbent assay(ELISA)was then used to quantify prostaglandin E2.PGE2.production upon knock-down of *** RT-qPCR analysis revealed that PACER was highly expressed in CRC tissues and cells,and a high PACER-expression level was associated with poor *** assay,wound-healing assay,colony-formation assay,and transwell assay revealed that PACER enhanced CRC-cell proliferation,invasion,and metastasis in *** of lncRNA localization by FISH showed that it mainly resided in the ***-qPCR showed that PACER increased mRNA levels of *** blot analysis demonstrated,und

关键词： p50-associated cyclooxygenase-2.extragenic RNA(PACER) colorectal cancer(CRC) lncRNA cyclooxygenase-2.COX-2.

Shortened Basal Internodes Encodes a Gibberellin 2.Oxidase and Contributes to Lodging Resistance in Rice

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molecular Plant 2018年第2期11卷 288-299页

作者： Chang liu Jinshan gui Chenjian Fu Hasi {2. Dongliang Song Junhui Shen Peng Qin Xuanming liu Bin Han {2.anzhu Yang Laigeng Li national key {2. of Plant molecular Genetics and CAS Center for Excellence in molecular Plant sciences Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai 200032 China yuan Longping Agriculture High-Tech Co. Ltd. Hunan 410001 China Hunan University Hunan 410082 China University of the chinese academy of sciences Beijing 100049 China national Center of Plant Gene Research and CAS Center for Excellence in molecular Plant {2. Institute of Plant Physiology and EcologyChinese Academy of Sciences Shanghai 200233 China Hunan Ava Seed Research institute Hunan 410119 China Hunan Engineering laboratory for Disease and Insect-Resistant Rice Breeding Hunan 410119 China

Breeding semi-dwarf varieties to improve lodging resistance has been proven to be enormously successful in increasing grain yield since the advent of the ＂green revolution.＂ However, the breeding of the majority of semi-dwarf rice varieties in Asia has been dependent mainly on genetic introduction of the mutant alleles of SD1, which encodes a gibberellin （GA） 2.-oxidase, OsGA2.ox2. for catalyzing GA biosynthesis. Here, we report a new rice lodging-resistance gene, Shortened Basal lnternodes （SBI）, which encodes a gibberellin 2. oxidase and specifically controls the elongation of culm basal internodes through deactivating GA activity. SBI is predominantly expressed in culm basal internodes. Genetic analyses indicate that SBI is a semidominant gene affecting rice height and lodging resistance. SBI allelic variants display different activities and are associated with the height of rice varieties. Breeding with higher activity of the SBI allele generates new rice varieties with improved lodging resistance and increased yield. The discovery of the SB! provides a desirable gene resource for producing semi-dwarf rice phenotypes and offers an effective strategy for breeding rice varieties with enhanced lodging resistance and high yield.

关键词： Shortened Basal Internodes gibberellin activity lodging resistance yield rice

Unraveling the impacts of intermolecular interaction on morphology evolution for highly efficient organic solar cells and modules

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Science china chemistry 2024年

作者： Xueqing Ma yingying Cheng {2.qiang liu Xinming Zheng Guangliu Ran Hongxiang Li Xin{2.e Cui Andong Zhang Wenkai Zhang Pei Cheng Wenchao Huang Zhishan Bo beijing key {2. of Energy Conversion and Storage Materials College of Chemistry Beijing Normal University College of Textiles and Clothing State Key Laboratory of Bio-fibers and Eco-textiles Qingdao University key State laboratory of Advanced Technology for Materials Synthesis and Processing School of Materials Science and EngineeringWuhan University of Technology Department of Physics and Applied Optics beijing Area Major laboratory Center for Advanced Quantum Studies Beijing Normal University State key laboratory of Polymer Materials Engineering College of Polymer Science and Engineering Sichuan University

Understanding the effect of intermolecular interaction on the growth dynamic of active layers is critical for advancing organic solar cells(OSCs). However, the diverse structure of donors and acceptors makes the research challenging. Additives with customizable structures and properties could simplify this complexity. Herein, we meticulously tailor two additives of 3,4-ethylenedioxythiophene(EDOT) and 2.5-dibromo-3,4-ethylenedioxythiophene(DBEDOT), possessing distinct intermolecular interaction features to elaborate the inherent relationship. It is found that varied interaction strengths can alter film formation processes. The enhanced intermolecular interaction between the DBEDOT and non-fullerene acceptor BTP-eC9-4F results in pre-aggregation and longer crystallization duration of BTP-eC9-4F, facilitating the formation of films with compact molecular packing and decent phase separation. Thus, exciton dissociation and charge transport become more efficient. Finally, devices processed with DBEDOT exhibit a remarkable power conversion efficiency of 19.35% in small-area OSCs and 14.11% in bladecoated 5 cm × 5 cm organic solar mini-modules. Especially, OSCs can maintain 80% of their initial efficiency after continuous annealing at 85 °C for over 2.100 h.

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An isoindigo-bithiazole-based acceptor-acceptor copolymer for balanced ambipolar organic thin-film transistors

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Science china chemistry 2016年第6期59卷 679-683页

作者： Ping Li Hanlin Wang Lanchao Ma Long Xu Fei Xiao Zhengran Yi yunqi liu Shuai Wang key laboratory for Large-Format Battery Materials and System Ministry of Education School of Chemistry and Chemical Engineering Huazhong University of Science and Technology Wuhan 430074 China Flexible Electronics Research Center State Key Laboratory of Digital Manufacturing Equipment and Technology School of Mechanical Science and Engineering Huazhong University of Science and Technology Wuhan 430074 China State key laboratory of organic solids Beijing National Laboratory for Molecular Sciences Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China

Balanced carrier transport is observed in acceptor-acceptor （A-A＇） type polymer for ambipolar organic thin-film transistors （OTFTs）. It is found that the incorporation of two electron-accepting moieties （BTz and IIG） into a polymer main chain to form A-A＇ polymer PIIG-BTz could lower highest occupied molecular orbital （HOMO） and lowest unoccupied molecular orbital （LUMO） levels and facilitate good molecular stacking of the polymer. Ambipolar transistor behaviour for PIIG-BTz, with the balanced hole and electron mobilities of 0.030 and 0.02. cm2.V 1 s-i was observed in OTFT devices, respectively. The study in this work reveals that the utilization of acceptor-acceptor （A-A＇） structure in polymer main chain can be a feasible strategy to develop ambipolar polymer semiconductors.

关键词： organic thin-film transistors acceptor-acceptor structure balanced ambipolar transport conjugated copolymer

The impact of reabsorption effect on composition analysis of organic semiconductors

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Science china Materials 2024年第10期67卷 3124-3131页

作者： Bei Yang Xiaoke liu Li Wan Weixin Ni Ni Yang Jianhui Hou Feng Gao Electronic and Photonic Materials(EFM) Department of PhysicsChemistry and Biology(IFM)Linkoping UniversityLinkoping SE-58183Sweden beijing national {2. for molecular sciences State Key Laboratory of Polymer and ChemistryCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China Max Planck institute of Microstructure Physic Halle 06120Germany

Reabsorption is one of the most fundamental optical phenomena,but it has rarely been considered in spectroscopy-based composition analysis for organic ***,we take four state-of-the-art organic solar cell(OSC)materials as examples,and systematically investigate the influence of reabsorption on photoluminescence emission and excitation spectra by both experimental studies and optical *** find that the overlap between absorption and emission spectra of these OSC materials is strong enough for them to be affected by the reabsorption effect,and the effect becomes more obvious between different species in the multi-components ***,three features of the reabsorption effect and the reabsorption strength are identified,with which we have successfully analyzed the composition in a range of OSC materials in both solution and solid-state *** work not only provides an important understanding of the largely overlooked feature of reabsorption in the widely used spectroscopic techniques but also offers an effective toolbox for the composition analysis of organic semiconductors.

关键词： reabsorption effect composition analysis organic solar cells non-fullerene acceptors organic semiconductors

Insights into the nitride-regulated processes at the electrolyte/electrode interface in quasi-solid-state lithium metal batteries

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Journal of Energy chemistry 2022年第4期31卷 780-786页

作者： Jing Wan Wan-Ping Chen gui-Xian liu Yang Shi Sen Xin {2.-Guo Guo Rui Wen Li-Jun Wan key laboratory of {2. Nanostructure and Nanotechnology Beijing National Laboratory for Molecular SciencesCAS Research/Education Center for Excellence in Molecular SciencesInstitute of ChemistryChinese Academy of SciencesBeijing 100190China University of chinese academy of sciences Beijing 100049China

Gel polymer electrolytes(GPEs)are one of the promising candidates for high-energy-density quasi-solid-state lithium metal batteries(QSSLMBs),for their high ionic conductivity and excellent interfacial *** comprehension of dynamic evolution and structure-reactivity correlation at the GPE/Li interface becomes ***,in situ electrochemical atomic force microscopy(EC-AFM)provides insights into the LiNO_(3)-regulated micromechanism of the Li plating/stripping processes upon cycles in GPE-based LMBs at *** additive LiNO_(3)induces the formation of amorphous nitride SEI film and facilitates Li^(+) ion *** stabilizes a compatible interface and regulates the Li nucleation/growth at steady *** deposited Li is in the shape of chunks and tightly *** Li dissolution shows favorable reversibility,which guarantees the cycling performance of *** situ AFM monitoring provides a deep understanding into the dynamic evolution of Li deposition/dissolution and the interphasial properties of tunable SEI film,regulating the rational design of electrolyte and optimizing interfacial establishment for GPE-based QSSLMBs.

关键词： In-situ electrochemical atomic force microscopy Gel polymer electrolyte Solid electrolyte interphase Lithium deposition/dissolution Quasi-solid-state lithium metal batteries

Potential regulation of linoleic acid and volatile organic compound contents in meat of chickens by PLCD1

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Journal of Integrative Agriculture 2023年第1期22卷 222-234页

作者： liu Xiao-jing WANG Yong-li liu Li liu Lu ZHAO gui-ping WEN Jie JIA Ya-xiong CUI Huan-xian State key laboratory of Animal Nutrition Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China

Omega-3(linolenic acid(ALA),docosapentaenoic acid,eicosapentaenoic acid)and omega-6(linoleic acid(LA),arachidonic acid)polyunsaturated fatty acids are essential for health and normal physiological functioning in *** we report a genome-wide association study(GWAS)on LA content in chicken *** 19 significant single nucleotide polymorphisms(SNPs)identified by the GWAS approach were annotated in VILL,PLCD1 and OXSR1 genes with highly polymorphic linkage blocks,and explained 4.5%of the phenotypic variation in the LA ***,the PLCD1 mRNA expression level was negatively correlated with the LA content,and significantly higher in chickens with low LA content than in those with high LA *** addition,PLCD1 was found to be involved in metabolic pathways,***,the LA content was correlated with volatile organic compounds(e.g.,octanal,etc.),but no relationship was found with intramuscular fat and triglycerides in chicken *** results indicated that there are {2. SNPs in PLCD1 that regulate the content of LA,and it has no significant effect on fat deposition,but may affect the content of volatile organic compounds(VOCs).

关键词： linoleic acid flavor quality intramuscular fat PLCD1 chicken meat