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Aptamer-Based Antibiotic Electrochemical Detection Platform Using Portable Plastic Gold Electrode and Multichannel Chip

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Chinese Journal of Chemistry 2024年第2期42卷 171-176页

作者： Yin Bao Kaiwei Cai yichen liu Bingling Li Changchun Institute of Applied Chemistry Chinese Academy of SciencesChangchunJilin 130022China School of Applied Chemistry and Engineering University of Science and Technology of ChinaHefeiAnhui 230026China

The development of accurate,rapid and practical electrochemical sensing technology for antibiotic detection has an important application prospect in many fields such as food safety,environmental monitoring,medical and health *** this research,kanamycin specific DNA aptamer and its partially complementary short chain were self-assembled onto the surface of an integrated portable plastic gold electrode(PGE)by Au-s bond,and a simple kanamycin electrochemical biosensor interface was *** the same time,the epoxy resin channel layer was designed,and the auxiliary instrument was set up independently in the laboratory,which could help to measure and analyze eight groups of samples automatically in *** quantitative analysis results showed that in 20 min,the sensor had a good linear relationship between the peak current change of square wave voltammetry and the negative logarithm of kanamycin concentration when kanamycin concentration was in the range of 1-10o0μmol/L,and the detection limit could reach 0.40μmol/*** addition,the discrimination could be achieved within 5 min in the real-time analysis *** sensor had a simple construction method,good selectivity and stability,and could be used for rapid or real-time detection of kanamycin residues in actual water samples,which provided a new direction for the practical detection of kanamycin in environmental watersamples.

关键词： Biosensor Aptamer Antibiotics Electroanalysis DNA

Long-and short-chain AHLs affect AOA and AOB microbial community composition and ammonia oxidation rate in activated sludge

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Journal of Environmental Sciences 2019年第4期31卷 53-62页

作者： Jie Gao Yu Duan Ying liu Xuliang Zhuang yichen liu Zhihui Bai Wenlin Ma Guoqiang Zhuang CAS Key Laboratory of Environmental Biotechnology Research Center for Eco-Environmental SciencesChinese Academy of Sciences College of Resources and Environment University of Chinese Academy of Sciences Beijing Enterprises Water Group Limited School of Life Sciences University of Science and Technology of China Beijing Climate Change Response Research and Education Center Department of Environment and Energy EngineeringBeijing University of Civil Engineering and Architecture

Quorum sensing(QS)regulation of the composition of ammonia-oxidising archaea(AOA)and ammonia-oxidising bacteria(AOB)communities and functions in wastewater treatment was ***,we explored the role of N-acyl-L-homoserine lactones(AHLs)in microbial community dynamics in activated *** average,the specific ammoniaoxidising-rate increased from 1.6 to 2.8 mg NH_4^+-N/g MLSS/hr after treatment with long-chain AHLs for 16 days,and the addition of AHLs to sludge resulted in an increased number of AOA/AOB amoA *** differences were observed in the AOA communities of control and AHL-treated cultures,but not the AOB ***,the dominant functional AOA strains of the Crenarchaeota altered their ecological niche in response to AHL *** results provide evidence that AHLs play an important role in mediating AOA/AOB microbial community parameters and demonstrate the potential for application of QS to the regulation of nitrogen compound metabolism in wastewater treatment.

关键词： Quorum sensing Activated sludge Ammonia-oxidising archaea Ammonia-oxidising bacteria Nitrogen removal Community composition

Molecular cloning and expression analysis of Crustin-like gene from Chinese shrimp Fenneropenaeus chinensis

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Acta Oceanologica Sinica 2008年第2期27卷 81-92页

作者： liu Fengsong LI Fuhua XIANG Jianhai DONG Bo liu yichen ZHANG Xiaojun ZHANG liusuo College of Life Science Hebei University Baoding 071002 China Experimental Marine Biology Laboratory Institute of Oceanology Chinese Academy of Sciences Qingdao 266071 China

A new member of antimicrobial protein genes of the Crustin family was cloned from haemocytes of the Chinese shrimp Fennero- penaeus chinensis by 3 ′and 5′ RACE. The full-length cDNA of Crustin-like gene contains a 390 bp open reading frame, encoding 130 amino acids. The deduced peptide contains a putative signal peptide of 17 amino acids and mature peptide of 113 amino acids. The molecular mass of the deduced mature peptide is 12. 3 ku. It is highly cationic with a theoretical isoelectric point of 8.5. The deduced amino acids sequence of this Crustin showed high homology with those of Penaeus （ Litopenaeus ） setferus. Northern blotting showed that the cloned Crustin gene was mainly expressed in haemocytes, gill, intestine, and RNA in situ hybridization indicated that the Crustin gene was constitutively expressed exclusively in haemocytes of these tissues. Capillary elec- trophoresis RT-PCR analysis showed that Crustin was up-regulated dramatically from 12 to 48 h after a brief decrease of mRNA during first 6 h in response to microbe infection. The level of Crustin mRNA began to restore at 72 h post-challenge. This indicated that Crustin gene might play an important role when shrimps are infected by bacterial pathogen.

关键词： Crustin-like gene Fenneropenaeius chinensis molecular cloning expression analysis microbe challenge

MicroRNA-9 as a paradoxical but critical regulator of cancer metastasis:Implications in personalized medicine

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Genes & Diseases 2021年第6期8卷 759-768页

作者： yichen liu Qiong Zhao Tao Xi Lufeng Zheng Xiaoman Li Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica School of PharmacyNanjing University of Chinese MedicineNanjingJiangsu Province210023PR China School of Life Science and Technology Jiangsu Key Laboratory of Carcinogenesis and InterventionChina Pharmaceutical University639 Longmian RoadNanjingJiangsu Province211198PR China

Metastasis,is a development of secondary tumor growths at a distance from the primary site,and closely related to poor prognosis and ***,there is still no effective treatment for metastatic ***,there is an urgent need to find an effective therapy for cancer *** of evidence indicates that miR-9 can function as a promoter or suppressor in cancer metastasis and coordinate multistep of metastatic *** this review,we summarize the different roles of miR-9 with the corresponding molecular mechanisms in metastasis of twelve common cancers and the multiple mechanisms underlying miR-9-mediated regulation of metastasis,benefiting the further research of miR-9 and metastasis,and hoping to bridge it with clinical applications.

关键词： Cancer Metastasis MiR-9 Oncogene Tumor suppressor

A review on emulsification via microfluidic processes

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Frontiers of Chemical Science and Engineering 2020年第3期14卷 350-364页

作者： yichen liu Yongli Li Andreas Hensel Juergen JBrandner Kai Zhang Xiaoze Du Yongping Yang Key Laboratory of Condition Monitoring and Control for Power Plant Equipment(Ministry of Education) North China Electric Power UniversityBeijing 102206China Institute for Micro Process Engineering Karlsruhe Institute of TechnologyEggenstein-Leopoldshafen 76344Germany Institute of Microstructure Technology Karlsruhe Institute of TechnologyEggenstein-Leopoldshafen 76344Germany

Emulsion is a disperse system with two immiscible liquids,which demonstrates wide applications in diverse *** technology has advanced well with the development of microfluidic *** to conventional methods,the micro-fluidics-based process can produce controllable droplet size and *** droplet formation or breakup is the result of combined effects resulting from interfacial tension,viscous,and inertial forces as well as the forces generated due to hydrodynamic pressure and extermal *** the current study,typical microfluidic systems,including microchannel array,T-shape,flow-focusing,co-flowing,and membrane systems,are reviewed and the corresponding mechanisms,fow regimes,and main parameters are compared and summarized.

关键词： microfluidics emulsification capillary num-ber droplet breakup

Thermoelectric performance enhancement by manipulation of Sr/Ti doping in two sublayers of Ca_(3)Co_(4)O_(9)

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Journal of Advanced Ceramics 2020年第6期9卷 769-781页

作者： Li ZHANG yichen liu Thiam Teck TAN Yi liu Jian ZHENG Yanling YANG Xiaojiang HOU Lei FENG Guoquan SUO Xiaohui YE Sean LI Shaanxi Key Laboratory of Green Preparation and Functionalization for Inorganic Materials School of Materials Science and EngineeringShaanxi University of Science&TechnologyXi’an 710021China UNSW Materials and Manufacturing Futures Institute School of Material Science and EngineeringThe University of New South WalesKensingtonNew South Wales2052Australia

Thermoelectric(TE)performance of Ca_(3)Co_(4)O_(9)(CCO)has been investigated extensively via a doping strategy in the past ***,the doping sites of different sublayers in CCO and their contributions to the TE performance remain unrevealed because of its strong correlated electronic *** this work,Sr and Ti are chosen to realize doping at the[Ca_(2)CoO_(3)]and[CoO_(2)]sublayers in *** was found that figure of merit(ZT)at 957 K of Ti-doped CCO was improved 30% than that of undoped CCO whereas 1 at% Sr doping brought about a 150% increase in ZT as compared to undoped *** significant increase in electronic conductivity and the Seebeck coefficient are attributed to the enhanced carrier concentration and spin-entropy of Co^(4+) originating from the Sr doping effects in[Ca_(2)CoO_(3)]sublayer,which are evidenced by the scanning electron microscope(SEM),Raman,Hall,and X-ray photoelectron spectroscopy(XPS)***,the reduced thermal conductivity is attributed to the improved phonon scattering from heavier Sr doped Ca site in[Ca_(2)CoO_(3)]*** findings demonstrate that doping at Ca sites of[Ca_(2)CoO_(3)]layer is a feasible pathway to boost TE performance of CCO material through promoting the electronic conductivity and the Seebeck coefficient,and reducing the thermal conductivity *** work provides a deep understanding of the current limited ZT enhancement on CCO material and provides an approach to enhance the TE performance of other layered structure materials.

关键词： layered structures manipulation doping sites Ca_(3)Co_(4)O_(9)(CCO) spin-entropy thermoelectric performance

A Study on Yeast Using the Photoreactivation Process to Repair the Pyrimidine Dimer Mutations

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Proceedings of Anticancer Research 2023年第4期7卷 49-56页

作者： yichen liu Shandong University Jinan 250100China

Sunlight has an indispensable importance for living things in nature[1-3].However,the direct absorption of UV will lead to the formation of pyrimidine dimers between adjacent pyrimidines in DNA strands usually in the form of cyclobutene pyrimidine dimers(CPDs)and pyrimidine(6-4)pyrimidone photoproducts(6-4PPs)which causes great damage[4-6].A DNA repair system,known as photoreactivation,can effectively repair the dimers using photolyase[7-9],which has currently been found in plants,prokaryotic and eukaryotic cells[10-12].This study was carried out to determine whether photolyase DNA repair can be observed in *** yeast Petri dishes were treated with ultraviolet radiation,different treatments were then added to them,and the colonies were counted after culturing,hence verifying that yeasts can use the photoreactivation process.

关键词： Photoreactivation DNA repair Photolyase

The Positive Effects of Physical Exercise on Mental Health Among Chinese Students Under Great Competitive Pressure

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Journal of Contemporary Educational Research 2023年第8期7卷 1-5页

作者： yichen liu Shandong University Jinan 250100Shandong ProvinceChina

Under an environment full of competitive stress,students tend to form negative emotions,and the time for them to engage in physical exercise is greatly limited due to overwhelming academic ***,in this paper,we focus on acknowledging the purpose of physical exercise,and how physical exercise affects the mental health of students under stressful circumstances but not in the context of *** was distributed to participants with an average age of 20,to investigate their preference,frequency,and reason for exercising,and their attitude towards *** results showed that participants think that exercise can strengthen the body,relieve stress,improve fitness and interpersonal relationship regardless of their purpose such as interests,emotional necessity and even out of non-subjective *** conclusion,physical exercise helped to improve students’mental health especially under great pressure and ultimately,it will enhance their academics and bring about the unity of mind and body.

关键词： Chinese students Physical exercise Purpose Positive effects Mental health

Maternal genetic history of southern East Asians over the past 12,000 years

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Journal of Genetics and Genomics 2021年第10期48卷 899-907页

作者： Yalin liu Tianyi Wang Xichao Wu Xuechun Fan Wei Wang Guangmao Xie Zhen Li Qingping Yang Peng Cao Ruowei Yang Feng liu Qingyan Dai Xiaotian Feng Wanjing Ping Bo Miao Yun Wu yichen liu Qiaomei Fu Key Laboratory of Vertebrate Evolution and Human Origins Institute of Vertebrate Paleontology and PaleoanthropologyCenter for Excellence in Life and PaleoenvironmentChinese Academy of SciencesBeijing 100044China Sino-Danish Center University of the Chinese Academy of SciencesBeijing 100049China University of the Chinese Academy of Sciences Beijing 100049China Northwest University Xi'an 710069China Fujian Longyan Museum Longyan 364000China International Research Center for Austronesian Archaeology Pingtan 350000China Fujian Museum Fuzhou 350001China Institute of Cultural Heritage Shandong UniversityQingdao 266237China Guangxi Institute of Cultural Relic Protection and Archaeology Nanning 530022China College of History Culture and TourismGuangxi Normal UniversityGuilin 541001China Yunnan Institute of Cultural Relics and Archaeology Kunming 650118China Archaeological Institute for Yangtze Civilization Wuhan UniversityWuhan 430072China

Southern East Asia,including Guangxi and Fujian provinces in China,is home to diverse ethnic groups,languages,and *** studies suggest a high complexity regarding population dynamics and the history of southern East ***,large-scale genetic studies on ancient populations in this region are hindered by limited sample ***,using highly efficient DNA capture techniques,we obtain 48 complete mitochondrial genomes of individuals from Guangxi and Fujian in China and reconstruct their maternal genetic history over the past 12,000 *** find a strong connection between southern East Asians dating to~12,000-6000 years ago and present-day Southeast *** addition,stronger genetic affinities to northern East Asians are observed in historical southern East Asians than Neolithic southern East Asians,suggesting increased interactions between northern and southern East Asians over ***,we reveal dynamic connections between ancient southern East Asians and populations located in surrounding regions,as well as a shift in maternal genetic structure within the populations over time.

关键词： Ancient DNA Southern East Asians Population history Maternal genetic structure