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MECHANISM OF INTERFERON ACTION——STRUCTURAL REQUIREMENT OF LIGAND AND CELLULAR SPECIFICITY

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Science in China,Ser.B 1985年第8期28卷 844-853页

作者：李伯良刘新垣 Shanghai Institute of Biochemistry Academia Sinica

We had reported the existence of 2’-5’P3A3 receptor on the peritoneal macrophages from Wistar rat. In this paper data are given to demonstrate that the ligand, for binding with 2’-5’P3A3 receptor and enhancing the phagocytosis of macrophages, must have a three-phosphate radical on its 5’-terminus and at least three adenylate residues in 2’-5’oligoadenylate. （3H）-2’-5’P3A3 can also bind on macrophages from other animals; and the binding of （3H）-2’-5’P3A3 on Hepatoma cell line 7402 is lower than that on macrophages; whereas it is very low （binding ratio less than 1%） on erythrocytes （from rabbit, rat and mouse） or human embryo lung fibroblast cells. The binding conditions of the ligand with 2’-5’P3A3 receptor have also been investigated.

关键词： MECHANISM OF INTERFERON ACTION STRUCTURAL REQUIREMENT OF LIGAND AND CELLULAR SPECIFICITY CCM than ADP

MECHANISM OF INTERFERON ACTION Ⅹ——ANTAGONISTIC EFFECT OF ATP ON pppA2’p5’A2’p5’A RECEPTOR

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中国科学B辑(英文版) 1988年第3期31卷 304-312页

作者：李世武刘新垣 Shanghai Institute of Biochemistry Academia Sinica Academia Sinica

pppA2’p5’A2’p5’A （2’-5’P3A3） receptor may be the first one in the oligonucleotide *** ATP can compete with 2’-5’P3A3 for the receptor. This raises a question whether this is anATP receptor. If not, what is the role of ATP for this receptor? Data available in this arti-cle show that ATP can bind to macrophages with saturability and reversibility. So ATPappears to be also a ligand of the receptor for 2’-5’P2A3. But the binding of ATP did notdevelop phagocytic effect as mentioned before[11]. Moreover, ATP inhibited the enhancementof phagocytosis of macrophages by 2’-5’P3A3 when it acted together with 2’-5’P3A3 on mac-rophages at the same time. It is concluded that ATP is an antagonist of 2’-5’P3A3 *** macrophages were treated with ATP prior to 2’-5’P3A3 and the binding sites for 2’-5’P3A3P were first occupied by ATP, both the binding ability and the biological effect of2’-5’P3A3 were all markedly blocked. The above data convincingly show that the action of2’-5’P3A3 is

关键词： macrophages ACTION markedly phagocytic inhibited blocked prior compete mentioned antagonist

一个革命性的抗癌研究策略:癌症的靶向基因-病毒治疗

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中国肿瘤生物治疗杂志 2015年第2期22卷 159-165页

作者：刘新垣中国科学院上海生命科学研究院生物化学与细胞生物学研究所上海200031

2001年,笔者提出了癌症的靶向基因-病毒治疗(cancer targeting gene-viro-therapy,CTGVT)的概念,即将一个抗癌基因插入到溶瘤病毒(oncolytic virus,OV)中,从而将基因治疗与溶瘤病毒各自的优势结合起来,由于溶瘤病毒能大量复制,插入其中... 详细信息

2001年,笔者提出了癌症的靶向基因-病毒治疗(cancer targeting gene-viro-therapy,CTGVT)的概念,即将一个抗癌基因插入到溶瘤病毒(oncolytic virus,OV)中,从而将基因治疗与溶瘤病毒各自的优势结合起来,由于溶瘤病毒能大量复制,插入其中的基因也能大量复制,故其抗肿瘤效果大增,较单用基因治疗或OV治疗强数十至上百倍。随后又引入双基因策略(CTGVT-DG),即向OV载体插入两个抗癌基因,由于两个抗癌基因之间可能存在互补或协同效应,在一些动物癌症模型中几乎能杀灭全部移植性肿瘤。近年来又采用纳米粒子把CTGVT-DG病毒颗粒包被,或者采用溶瘤痘病毒(OncoPox)作为载体与CTGVT-DG策略相结合,后者将可构建一系列抗癌作用极好的双基因OncoPox-gene1-gene2产物。国外多数使用OV-GM-CSF策略,仅局限于GM-CSF的免疫功能,忽视了基因复制的剂量效应及其重要性,这就是中国的CTGVT-DG胜过西方的优势之处。

关键词：癌症溶瘤病毒治疗基因治疗基因-病毒治疗双基因治疗

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资金短缺零售商促销下供应链的协调

管理工程学报 2022年第3期36卷 189-202页

作者：代建生刘新愿昆明理工大学管理与经济学院云南昆明650093

在零售商资金短缺且实施促销努力下,本文探讨了零售商的运营策略及供应链的协调问题。本文构造了一个衡量资金稀缺程度的指标,利用这一指标分析了资金稀缺度对零售商订货及促销策略的影响,结果表明:存在一个临界值,当资金稀缺度大于这... 详细信息

在零售商资金短缺且实施促销努力下,本文探讨了零售商的运营策略及供应链的协调问题。本文构造了一个衡量资金稀缺程度的指标,利用这一指标分析了资金稀缺度对零售商订货及促销策略的影响,结果表明:存在一个临界值,当资金稀缺度大于这个临界值时,订货量(促销强度)关于资金稀缺度递增(递减);当资金稀缺度小于临界值时,零售商的策略不随资金稀缺度的变化而变化。本文在允许和不允许单边转移支付两种情形下,分别设计了五种能协调供应链的收益共享和促销成本分担机制,其中供应商分担的促销成本可及时结算或延后结算。如果延后结算促销成本,仅当初始资金大于集中决策下的最优促销成本时,渠道协调才可实现;如果及时结算,则不管资金有多么稀缺,始终有一种契约机制能协调供应链。

关键词：供应链协调资金约束延迟支付促销

基于位置替代法的无线电引信混响室敏感度测试方法

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高电压技术 2015年第1期41卷 320-326页

作者：熊久良刘心愿军械工程学院静电与电磁防护研究所石家庄050003

针对引信敏感度分步测试方法工作量大、测试时间长等问题,提出了基于位置替代法的无线电引信混响室敏感度测试方法。利用混响室统计均匀的特性,将场强测试设备与受试设备同时置于混响室内进行测试,用测试位置平均场强替代引信受试位置场... 详细信息

针对引信敏感度分步测试方法工作量大、测试时间长等问题,提出了基于位置替代法的无线电引信混响室敏感度测试方法。利用混响室统计均匀的特性,将场强测试设备与受试设备同时置于混响室内进行测试,用测试位置平均场强替代引信受试位置场强,从而得到引信敏感度阈值。试验结果表明:各测试频点在不同采样点处测量值的平均值与均匀场测试结果相关性最好,相关系数可达95%,最小值与均匀场测试结果误差最小,其相对误差在3 d B范围内。与分步测试方法相比,该测试方法避免了试验中对受试设备的移动,减小了工作量,具有更好的实用性。

关键词：敏感度测试分步测试法位置替代法混响室无线电引信归一化场强

Efficient electrocatalytic reduction of nitrate to ammonia at low concentration by copper-cobalt oxide nanowires with shell-core structure

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Nano Research 2024年第6期17卷 5087-5094页

作者： Chenhao Xiao Yaxiao Guo Jiayuan Sun Tao Guo xinyuan Jia Shinuo Guo Guancheng Wu Yue Sun Zhaoyang Yao Yi liu State Key Laboratory of Separation Membranes and Membrane Processes School of ChemistryTiangong UniversityTianjin 300387China Key Laboratory of Functional Polymer Materials College of ChemistryNankai UniversityTianjin 300071China School of Chemical and Environmental Engineering Wuhan Polytechnic UniversityWuhan 430023China

Electrocatalytic nitrate reduction to ammonia(NO3−RR)for removing nitrate from wastewater is a promising but challengeable technology that is increasingly ***,we developed an efficient CuO_(x)and CoCuO_(x)composed hybrid catalyst(CoCuO_(x)@CuO_(x)/copper foam(CF)),characteristic of distinctive shell-core nanowires grown on CF substrate with CuO_(x)core and CoCuO_(x)*** built-in electric field formed at the interface of the CoO/Cu_(2)O heterostructure promotes NO3−adsorption by modulating the charge distribution at the interface,which greatly improves the ammonia yield rate and Faradaic ***−0.2 V *** hydrogen electrode(RHE),CoCuO_(x)@CuO_(x)/CF achieves not only an excellent ammonia yield rate of up to 519.1μg·h^(−1)·cm^(−2)and Faradaic efficiency of 99.83%at 1 mM NO3−concentration,but also excellent mechanical *** study provides a novel pathway to design electrocatalyst for the removal of nitrate from dilute nitric acid solutions(≤2 mM).

关键词： CoCuO_(x)@CuO_(x)/copper foam(CF) shell-core nanowires heterostructure low concentration nitrate electroreduction to ammonia

成纤维细胞生长因子2通过抑制TET2/UQCRH表达拮抗血管平滑肌细胞凋亡和促进其增殖

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中国动脉硬化杂志 2024年第10期32卷 843-849页

作者：徐睿妍李雯柳新嫄姚童屈顺林危当恒王佐姜志胜李国华南华大学心血管疾病研究所动脉硬化学湖南省重点实验室湖南省动脉硬化性疾病国际科技创新合作基地湖南省衡阳市421001 南华大学附属第七医院湖南省荣军优抚医院湖南省长沙市410000

[目的]探讨10-11转位蛋白2(TET2)/泛醌细胞色素C还原酶铰链蛋白(UQCRH)轴在成纤维细胞生长因子2(FGF-2)抑制血管平滑肌细胞(VSMC)凋亡中的作用。[方法]正常培养的VSMC分为对照组、FGF-2组、FGF-2+成纤维细胞生长因子受体(FGFR)泛抑制剂L... 详细信息

[目的]探讨10-11转位蛋白2(TET2)/泛醌细胞色素C还原酶铰链蛋白(UQCRH)轴在成纤维细胞生长因子2(FGF-2)抑制血管平滑肌细胞(VSMC)凋亡中的作用。[方法]正常培养的VSMC分为对照组、FGF-2组、FGF-2+成纤维细胞生长因子受体(FGFR)泛抑制剂LY2874455组。TET2基因过表达(OETET2)或UQCRH基因过表达(OEUQCRH)的VSMC分为对照组、FGF-2组、OETET2+FGF-2组或OEUQCRH+FGF-2组。采用Hoechst33342和PI染色检测细胞凋亡,CCK-8实验检测细胞增殖,Western blot检测凋亡相关蛋白pro-Caspase-3、cleaved Caspase-3、Bax、Bcl-2及TET2、UQCRH表达水平。运用NCBI、methprimer网站预测分析UQCRH基因启动子CpG岛位点。[结果]FGF-2可抑制VSMC凋亡、促进其增殖,下调促凋亡相关蛋白cleaved Caspase-3、Bax和TET2、UQCRH表达,上调抗凋亡蛋白Bcl-2表达(与对照组相比,P0.05),LY2874455可对抗FGF-2的作用(与FGF-2组相比,P<0.05)。TET2或UQCRH过表达可逆转FGF-2对VSMC抗凋亡、促增殖的作用,促进促凋亡相关蛋白表达上调,抗凋亡蛋白表达下调(与FGF-2组相比,P<0.05)。UQCRH基因启动子区域存在3个CpG岛。过表达TET2能上调FGF-2处理的VSMC中UQCRH表达(与FGF-2组相比,P<0.05)。[结论]FGF-2通过抑制TET2和UQCRH表达,减少VSMC凋亡,促进其增殖。

关键词：成纤维细胞生长因子2 10-11转位蛋白2 泛醌细胞色素C还原酶铰链蛋白细胞凋亡细胞增殖

Improving anammox activity and reactor start-up speed by using CO_(2)/NaHCO_(3) buffer

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Journal of Environmental Sciences 2024年第5期139卷 60-71页

作者： Shaoan Cheng Huahua Li xinyuan He Hua Chen Longxin Li State Key Laboratory of Clean Energy Utilization College of Energy EngineeringZhejiang UniversityHangzhou 310027China

Anammox bacteria grow slowly and can be affected by large pH *** suitable buffers could make the start-up of anammox reactors easy and *** this study,the effects of three kinds of buffers on the nitrogen removal and growth characteristics of anammox sludge were *** with CO_(2)/NaHCO_(3)buffer solution(CCBS)performed the best in nitrogen removal,while 4-(2-hydroxyerhyl)piperazine-1-ethanesulfonic acid(HEPES)and phosphate buffer solution(PBS)inhibited the anammox *** with 50 mmol/L CCBS could start up in 20 days,showing the specific anammox activity and anammox activity of 1.01±0.10 gN/(gVSS·day)and 0.83±0.06 kgN/(m^(3)·day),*** Kuenenia was the dominant anammox bacteria,with a relative abundance of 71.8%.Notably,anammox reactors could also start quickly by using 50 mmol/L CCBS under nonstrict anaerobic *** findings are meaningful for the quick start-up of engineered anammox reactors and prompt enrichment of anammox bacteria.

关键词： Anammox bacteria Buffer solution CO_(2)/NaHCO_(3) Physiological characteristics Microbial community shift

MECHANISM OF INTERFERON ACTION——THE DISCOVERY OF pppA2’p5’A2’p5’A RECEPTOR

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Science in China,Ser.B 1985年第7期28卷 697-706页

作者：李伯良刘新垣 Shanghai Institute of Biochemistry Academia Sinica

In this paper it is demonstrated that （3H）2’-5’P3A3 can bind to the peritoneal macrophages from Wistar rat. This binding is strongly inhibited by cold 2’-5’P3A3 while the inhibiting capacity of 2’-5’P3I3 and 2’-5’A3 is very small, showing that this binding is highly specific. And the binding of （3H）2’-5’P3A3 is reversible, saturable and with high affinity. A Scatchard analysis of the binding data gives a linear plot, an apparent dissociation constant （Kd） about 1.3×10-7 M and binding sites per cell about 2.1×107. The above evidence shows the existence of the 2’-5’P3A3 receptor, a fact which, to our knowledge, has not been reported before.

关键词： MECHANISM OF INTERFERON ACTION THE DISCOVERY OF pppA2’p5’A2’p5’A RECEPTOR